pycom03g03120

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002116
Physical Location & Seq
Forward (+)
2462678 .. 2465155
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1350 bp
ATGGCAGTCCAACACCCTCTTGAGTTAGCTAATACTGGTTCCTGTGATGATGATGGCCATCCACTACGAACTGGAACTCTATGGAGTTGTGTGGCTCATATCATCACTGCTGTGATTGGATCTGGAGTTCTGTCTTTGGCATGGAGTACTGCACAGCTAGGCTGGATTGGAGGGCCAGTTTGTTTGCTTAGTTTTGCGATTGTCACCTACATTTCTTCCTTCCTCCTTTCGGATTGTTACCGGTCTCCTGATCCTATCACCGGAACTCGAAACAAATCTTACATGAATGCTGTGAAAGTGAATCTCAATAGTATAAAAAAGACCTGGTTTTGTGGTTTCCTTCTATATTTCAGCATGTTTGGAACTGGTATTGCTTATGTGATTACCACTGCAACCAGTATGAGAGCCATTCAGAGATCAAACTGCTATCACAAAGAAGGGCATGAAGCTTCCTGTCAATATGGGACTGGTTTGTACATGATGCTATTTGGACTTGTCCAGATTGTAGTGTCACAAATACCAGATTTCCACAACATGAAATGGCTATCAGTTGTTGCAGCAATCATGTCGTTTGCCTACTCTTTCATTGGACTTGGCCTCGGCATTGCGAAATCAATAGAAAATGGGAGGATTCAAGGAAGCCTGGCAGGAGTGCCAACCTCTAATGTTGCTGATAAATTATGGTTAGTCTTCCAAGCACTTGGAGACATCGCTTTTGCCTATCCGTACTCTATTATTCTTCTTGAAATTCAGACAATGAAGAAGGCCTCAATGATTGCCATATTTGTCACAACCTTCTTCTACCTCTGCTGTGGATGCTTTGGATACGCCGCCTTTGGGAATGACACGCCCGGGAACCTCTTGACAGGATTCGGATTCTACGAACCCTACTGGCTCATTGATTTTGCTAATGCTTGCATTGTTCTTCATTTGGTGGGAGGATATCAGGTCTACAGTCAACCAGTATTTGCAGTTGCTGAGAGATGGTCTAGGAAGAAATACCCAAACAGCGGATTTGTGAACAACTTTTACTCCATCAAAATCCCATTGTTGCCAAGATTTCAAGTGAATCCCTTCAGGCTGTGTTTCCGAACTGTTTACGTTGTGTCAACTACCGCAATTGCAATGATATTTCCTTACTTCAACCAGGTTTTGGGAGTTTTAGGGGCCTTGAACTTTTGGCCTTTGGCTATATATTTCCCTGTTGAAATGTACTTTGTACAAAAGAAAATCGGGGCTTGGACAAAAAAATGGATTGTTCTAAGGACATTTAGTTTAGTTTGCTTTCTTGTGACGGTTGTGGGTGTGATTGGATCAGTTGAAGGACTTATTAGTGCCAAACTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

49.69

Weight (kDa)

8.99

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 24 - 444 1.8e-93 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1153
AccI GTMKAC 1 cut(s) 951
AciI CCGC 3 cut(s) 831, 1011, 1116
AclWI GGATC 3 cut(s) 127, 245, 1321
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 1060
AfaI GTAC 5 cut(s) 148, 476, 728, 1214, 1221
AfiI CCNNNNNNNGG 5 cut(s) 229, 260, 837, 1010, 1153
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 7 cut(s) 635, 746, 1064, 1144, 1174, 1208, 1322
AjnI CCWGG 3 cut(s) 323, 642, 1146
AleI CACNNNNGTG 1 cut(s) 110
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 4 cut(s) 29, 157, 449, 1347
AluI AGCT 4 cut(s) 29, 157, 449, 1347
Alw26I GTCTC 2 cut(s) 249, 699
AlwI GGATC 3 cut(s) 127, 245, 1321
AlwNI CAGNNNCTG 1 cut(s) 977
Ama87I CYCGRG 1 cut(s) 851
AoxI GGCC 6 cut(s) 55, 173, 595, 765, 1167, 1181
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 1 cut(s) 747
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AsiGI ACCGGT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 1073
AspS9I GGNCC 2 cut(s) 173, 1167
AsuC2I CCSGG 2 cut(s) 852, 853
AsuHPI GGTGA 2 cut(s) 196, 250
AvaI CYCGRG 1 cut(s) 851
BalI TGGCCA 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 682
BbvI GCAGC 1 cut(s) 569
BccI CCATC 4 cut(s) 47, 66, 978, 1043
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 3 cut(s) 325, 644, 1148
BciVI GTATCC 1 cut(s) 818
BcnI CCSGG 2 cut(s) 852, 853
BcoDI GTCTC 2 cut(s) 249, 699
BfaI CTAG 3 cut(s) 158, 990, 1348
BfmI CTRYAG 1 cut(s) 952
BfuI GTATCC 1 cut(s) 818
BisI GCNGC 2 cut(s) 558, 831
BlsI GCNGC 2 cut(s) 559, 832
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 5 cut(s) 325, 644, 852, 853, 1148
BmeT110I CYCGRG 1 cut(s) 851
BmgT120I GGNCC 2 cut(s) 173, 1167
BmiI GGNNCC 3 cut(s) 40, 857, 1168
BmrFI CCNGG 5 cut(s) 325, 644, 852, 853, 1148
BmsI GCATC 2 cut(s) 471, 806
BpiI GAAGAC 1 cut(s) 682
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 41
BpuMI CCSGG 2 cut(s) 852, 853
BsaBI GATNNNNATC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 2 cut(s) 598, 851
BsaWI WCCGGW 2 cut(s) 240, 260
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 1016, 1046
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 260, 837, 1010, 1153
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 8 cut(s) 40, 76, 176, 370, 396, 472, 896, 962
Bse3DI GCAATG 2 cut(s) 603, 1131
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 3 cut(s) 325, 644, 1148
BseDI CCNNGG 2 cut(s) 598, 851
BseGI GGATG 2 cut(s) 58, 821
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 5 cut(s) 229, 260, 837, 1010, 1153
BseMI GCAATG 2 cut(s) 603, 1131
BseMII CTCAG 1 cut(s) 969
BseNI ACTGG 8 cut(s) 40, 76, 176, 370, 396, 472, 896, 962
BseXI GCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 6 cut(s) 57, 175, 597, 767, 1169, 1183
BshTI ACCGGT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 851
BsiSI CCGG 3 cut(s) 241, 261, 852
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 5 cut(s) 229, 260, 837, 1010, 1153
BsmAI GTCTC 2 cut(s) 249, 699
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 6 cut(s) 57, 175, 597, 767, 1169, 1183
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 851
Bsp1407I TGTACA 2 cut(s) 474, 1219
Bsp143I GATC 4 cut(s) 119, 250, 416, 1313
BspACI CCGC 3 cut(s) 831, 1011, 1116
BspANI GGCC 6 cut(s) 57, 175, 597, 767, 1169, 1183
BspCNI CTCAG 1 cut(s) 970
BspLI GGNNCC 3 cut(s) 40, 857, 1168
BspPI GGATC 3 cut(s) 127, 245, 1321
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 2 cut(s) 603, 1131
BsrFI RCCGGY 1 cut(s) 240
BsrGI TGTACA 2 cut(s) 474, 1219
BsrI ACTGG 8 cut(s) 40, 76, 176, 370, 396, 472, 896, 962
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 598, 851
BssMI GATC 4 cut(s) 119, 250, 416, 1313
Bst2UI CCWGG 3 cut(s) 325, 644, 1148
Bst4CI ACNGT 3 cut(s) 956, 1096, 1297
BstAUI TGTACA 2 cut(s) 474, 1219
BstC8I GCNNGC 1 cut(s) 916
BstDEI CTNAG 4 cut(s) 188, 978, 1262, 1343
BstF5I GGATG 2 cut(s) 58, 821
BstKTI GATC 4 cut(s) 122, 253, 419, 1316
BstMAI GTCTC 2 cut(s) 249, 699
BstMBI GATC 4 cut(s) 119, 250, 416, 1313
BstMWI GCNNNNNNNGC 1 cut(s) 816
BstNI CCWGG 3 cut(s) 325, 644, 1148
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 5 cut(s) 323, 642, 850, 851, 1146
BstSFI CTRYAG 1 cut(s) 952
BstV1I GCAGC 1 cut(s) 569
BstV2I GAAGAC 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 701
BstYI RGATCY 1 cut(s) 119
BsuI GTATCC 1 cut(s) 818
BsuRI GGCC 6 cut(s) 57, 175, 597, 767, 1169, 1183
BtgZI GCGATG 1 cut(s) 694
BtsCI GGATG 2 cut(s) 58, 821
BtsI GCAGTG 2 cut(s) 105, 387
BtsIMutI CAGTG 2 cut(s) 105, 387
Cac8I GCNNGC 1 cut(s) 916
CaiI CAGNNNCTG 1 cut(s) 977
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 173, 1167
Cfr9I CCCGGG 1 cut(s) 851
CsiI ACCWGGT 2 cut(s) 323, 1146
Csp6I GTAC 5 cut(s) 147, 475, 727, 1213, 1220
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 7 cut(s) 141, 283, 355, 443, 478, 535, 565
CviQI GTAC 5 cut(s) 147, 475, 727, 1213, 1220
DdeI CTNAG 4 cut(s) 188, 978, 1262, 1343
DpnI GATC 4 cut(s) 121, 252, 418, 1315
DpnII GATC 4 cut(s) 119, 250, 416, 1313
EaeI YGGCCR 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 767
Eco31I GGTCTC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 944
Eco57I CTGAAG 1 cut(s) 1060
Eco88I CYCGRG 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 1167
EcoRII CCWGG 3 cut(s) 323, 642, 1146
EcoRV GATATC 1 cut(s) 944
FaeI CATG 7 cut(s) 144, 286, 358, 446, 481, 538, 568
FaqI GGGAC 1 cut(s) 478
FatI CATG 7 cut(s) 140, 282, 354, 442, 477, 534, 564
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 2 cut(s) 558, 831
FokI GGATG 2 cut(s) 45, 828
Fsp4HI GCNGC 2 cut(s) 558, 831
FspBI CTAG 3 cut(s) 158, 990, 1348
GluI GCNGC 2 cut(s) 558, 831
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 6 cut(s) 57, 175, 597, 767, 1169, 1183
HapII CCGG 3 cut(s) 241, 261, 852
Hin1II CATG 7 cut(s) 144, 286, 358, 446, 481, 538, 568
HincII GTYRAC 2 cut(s) 959, 1110
HindII GTYRAC 2 cut(s) 959, 1110
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 5 cut(s) 301, 631, 870, 876, 1069
HpaII CCGG 3 cut(s) 241, 261, 852
HphI GGTGA 2 cut(s) 196, 250
Hpy166II GTNNAC 5 cut(s) 952, 959, 1021, 1099, 1110
Hpy188I TCNGA 5 cut(s) 232, 414, 753, 875, 1091
Hpy188III TCNNGA 6 cut(s) 20, 123, 248, 499, 743, 862
Hpy8I GTNNAC 5 cut(s) 952, 959, 1021, 1099, 1110
HpyAV CCTTC 7 cut(s) 229, 350, 431, 757, 805, 1084, 1316
HpyCH4III ACNGT 3 cut(s) 956, 1096, 1297
HpyCH4IV ACGT 1 cut(s) 1101
HpyCH4V TGCA 6 cut(s) 152, 392, 557, 918, 971, 1124
HpyF10VI GCNNNNNNNGC 1 cut(s) 816
HpyF3I CTNAG 4 cut(s) 188, 978, 1262, 1343
HpySE526I ACGT 1 cut(s) 1101
Hsp92II CATG 7 cut(s) 144, 286, 358, 446, 481, 538, 568
Kzo9I GATC 4 cut(s) 119, 250, 416, 1313
Lsp1109I GCAGC 1 cut(s) 569
LweI GCATC 2 cut(s) 471, 806
MabI ACCWGGT 2 cut(s) 323, 1146
MaeI CTAG 3 cut(s) 158, 990, 1348
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 5 cut(s) 202, 236, 510, 787, 1291
MalI GATC 4 cut(s) 121, 252, 418, 1315
MboI GATC 4 cut(s) 119, 250, 416, 1313
MboII GAAGA 7 cut(s) 207, 682, 731, 772, 790, 917, 1006
MfeI CAATTG 1 cut(s) 1119
MflI RGATCY 1 cut(s) 119
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 3 cut(s) 677, 747, 1119
MluNI TGGCCA 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 34
Mox20I TGGCCA 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 1073
MscI TGGCCA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 57
MspA1I CMGCKG 1 cut(s) 1011
MspI CCGG 3 cut(s) 241, 261, 852
MspR9I CCNGG 5 cut(s) 325, 644, 852, 853, 1148
MunI CAATTG 1 cut(s) 1119
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 3 cut(s) 325, 644, 1148
MwoI GCNNNNNNNGC 1 cut(s) 816
NciI CCSGG 2 cut(s) 852, 853
NdeII GATC 4 cut(s) 119, 250, 416, 1313
NlaIII CATG 7 cut(s) 144, 286, 358, 446, 481, 538, 568
NlaIV GGNNCC 3 cut(s) 40, 857, 1168
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 4 cut(s) 202, 510, 787, 1291
NspI RCATGY 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 110
PceI AGGCCT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PctI GAATGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 5 cut(s) 301, 631, 870, 876, 1069
PflMI CCANNNNNTGG 1 cut(s) 1153
PinAI ACCGGT 1 cut(s) 240
PkrI GCNGC 2 cut(s) 559, 832
Psp6I CCWGG 3 cut(s) 323, 642, 1146
PspGI CCWGG 3 cut(s) 323, 642, 1146
PspN4I GGNNCC 3 cut(s) 40, 857, 1168
PspPI GGNCC 2 cut(s) 173, 1167
PsrI GAACNNNNNNTAC 2 cut(s) 1013, 1045
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 5 cut(s) 148, 476, 728, 1214, 1221
RsaNI GTAC 5 cut(s) 147, 475, 727, 1213, 1220
RseI CAYNNNNRTG 1 cut(s) 110
SatI GCNGC 2 cut(s) 558, 831
Sau3AI GATC 4 cut(s) 119, 250, 416, 1313
Sau96I GGNCC 2 cut(s) 173, 1167
ScaI AGTACT 1 cut(s) 148
ScrFI CCNGG 5 cut(s) 325, 644, 852, 853, 1148
SexAI ACCWGGT 2 cut(s) 323, 1146
SfaNI GCATC 2 cut(s) 471, 806
SfcI CTRYAG 1 cut(s) 952
SmaI CCCGGG 1 cut(s) 853
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 3 cut(s) 677, 747, 1119
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 3 cut(s) 831, 1011, 1116
SspMI CTAG 3 cut(s) 158, 990, 1348
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 5 cut(s) 323, 642, 850, 851, 1146
TaaI ACNGT 3 cut(s) 956, 1096, 1297
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 1 cut(s) 268
TasI AATT 3 cut(s) 677, 747, 1119
TatI WGTACW 4 cut(s) 146, 474, 1212, 1219
TauI GCSGC 1 cut(s) 833
TfiI GAWTC 5 cut(s) 301, 631, 870, 876, 1069
TscAI CASTG 2 cut(s) 112, 394
TseFI GTSAC 4 cut(s) 202, 510, 787, 1291
TseI GCWGC 1 cut(s) 557
Tsp45I GTSAC 4 cut(s) 202, 510, 787, 1291
TspDTI ATGAA 6 cut(s) 299, 459, 551, 574, 773, 917
TspGWI ACGGA 1 cut(s) 714
TspMI CCCGGG 1 cut(s) 851
TspRI CASTG 2 cut(s) 112, 394
Van91I CCANNNNNTGG 1 cut(s) 1153
XapI RAATTY 1 cut(s) 747
XceI RCATGY 1 cut(s) 358
XmaI CCCGGG 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 3 cut(s) 158, 990, 1348
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.