pycom03g13690

cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006731
Physical Location & Seq
Forward (+)
14797960 .. 14800107
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13690.1

Sequence Viewer

Length: 2148 bp
ATGGATGAAGGCGACGGGGCTGATCAAATGGATCAGTTCCACCGCAACGAGGCGATCTCGGCGGTGGCCGACGATGGTTTTTTGGCCGAGGAGGACGACGATTATGAGGACCTTTACAACGACGTCAATGTCGGCGAGGGGTTTTATCAGTCTATGAGAAAGAACGAGGATGTAGGGTTTAGGAATGAGGTGGTGGAGGAGAAGAAAGTTGAGGTTGAGTCACCAGATGTTGCTCCGTCGGAATCTCAAGGTGGTCTGATACCCGGTAGTGGTGAGGAGACCGGTGGGGGTGGGGGTGGGGGTGGGGGTGATGGAGGGGTTAGAGGTTCTGGGTATAATAATCAGAATCTAGAGTTTAGAGGGAACGATATGGGTATGAAAGGGAGTGGTGGGTTGGGGCCCGTTGGGGGCGGGGGAGGAGGTGTGATTAGGGTTGAATTAGGGCAGGCATCGAGTAAGAAGAATGTGTTTGAGCAACAAAGTGGGAATAATAGTAGTGTTGGGGTGCAAGGGATTGTTGCTCAGCAGCCGCCACAACAATTGCAACAACAACATCAACCACATTCTCATCCGCAACAGCAACAGTTACATGGTCCTGCTGCTGGTGGGAATGTGGGGAATGAGGGCATGTTGAGGCAAGCTGGTAGTGTTGGAGGTGGTAACATGAATGGGATTGGTGGGAATAGTGGTCCGGGAGGAGGATTTGGTGGTGCCGCTGGTGGTGGAGCGGGGACAATATTGTTTGTGGGTGATTTGCATTGGTGGACTACTGATGCAGAGCTGGAGGCAGAGTTATGTAAGTATGGACCTGTTAAGGAAGTGAAGTTTTTCGATGAGAAAGCAAGTGGAAAGTCAAAAGGGTACTGTCAGGTTGAGTTTTATGATCCTAATTCTGCCACGGCCTGCAAGGAAGGGATGCATGGTCATGTGTTTAATGGGCGCCCATGCGTTGTTGCATTTGCATCACCATTTAGTGTTAAGAGAATGGGGGAGGCTCAAGTAAATAGGAACCAGCAGGTAACCCAAACTGCAGTGCCTCAAATTGGTGGGAGGAGGGGATCCAATGAAGCCGGGAATAAAACTGGTGGGAGTAACATTTCAACCGGCGGGAATTATCAAGGTGGGGATAACAATAGAGGTTATGGGAGAGGTAACTGGGGGAGAGGTAATACTCAGGGTATGGGAAATAGAGGACCAGTTGGACCAAGGAATAGGGGTGCGATGAATGGAAGGGGTATAATGGGTAATGGTGGAAATGGGTTTGGGCAAGGTATTGGTGCAACCCCTCCTATGATGCACCCTCAGTCAATGATGGGTCAGGGTTTTGATCCAGCTTTTGGTGGACCAATGGGGAGAATGGGAAGTTATGGAGGCTTTCCTGGTGCTCCTACACCTCCTTTCTCAGGAATCTTGTCTTCATTTCCACCTGTGGGGAATGTTGGGCTACCTGGAGTGGCCCCTCATGTGAATCCTGCATTCTTTGGAAGAGGAATGCCCATGAATGGAATGGGAATGATGCCTACATCAGGTGTTGATGGGCCTAATATGGGTATGTGGTCCGACCCTAGTATGGGTGGATGGGCTGGTGAAGAGCATGGTGGTGGCAGAGCTGGGGAGTCTAGTTATGGCGAGGAAGCTGCTTCTGACCATCAATATGGTGAGGTTAGTCATGATAGAGGAGGTGCATGGCCAAATGCCATGAAGGAGAAAGATAGAGGTTCAGAGAGGGACTGGTCTGGATCTTCTGATAGAAGATATCGGGATGATAGGGATCAGGGATATGAGAGAGACGCGCCTAGAGAGAAAGAGGTGGCTCATGACCATGACTTCTCTGAAAGAAGGTATCGTGATGATGTTGATGTTAGTCGAGAACGGGACAGAGAGCGTGATAGGGGTCGAGAACGTTCTCGAGATCATGATCGTGAACGTGATAAAAATCGTGAACGAGACCGTGACAGACACAGGGAAGACAGAGACAGATATGCTGATCATCATAAGTACAGAGACCGTGAGCAGGAACATGATGATGAATGGGAACGGGGACGGTCATCCAGGCATAGTAAGGCACGTGTAACTCAAGAGGATGACCGTCGGTCAAGATCAAGGGATGCTGATTATGGGAAGAGAAGGCGGCTTACTTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000381 GO:0000785 GO:0000790 GO:0000791 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005694 GO:0005719 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006508 GO:0006509 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022607 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0031982 GO:0032774 GO:0032991 GO:0033119 GO:0033120 GO:0033619 GO:0034097 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043230 GO:0043231 GO:0043232 GO:0043233 GO:0043484 GO:0043565 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044530 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048024 GO:0048025 GO:0048026 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050685 GO:0050686 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051259 GO:0051260 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070062 GO:0070555 GO:0070887 GO:0071013 GO:0071310 GO:0071345 GO:0071347 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902680 GO:1903311 GO:1903312 GO:1903313 GO:1903506 GO:1903508 GO:1903561 GO:1990837 GO:1990904 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
RRM_1

Protein Analysis

716

Amino Acids

76.83

Weight (kDa)

5.59

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 247 - 316 5.8e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 1006
AccB1I GGYRCC 2 cut(s) 710, 939
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccBSI CCGCTC 1 cut(s) 728
AccII CGCG 1 cut(s) 1793
AciI CCGC 9 cut(s) 43, 62, 411, 530, 572, 714, 728, 1107, 2131
AclI AACGTT 1 cut(s) 1903
AclWI GGATC 7 cut(s) 39, 878, 1053, 1066, 1322, 1747, 1779
AcoI YGGCCR 3 cut(s) 66, 84, 1688
AcvI CACGTG 1 cut(s) 2069
AcyI GRCGYC 2 cut(s) 123, 940
AfaI GTAC 2 cut(s) 863, 2000
AflIII ACRYGT 1 cut(s) 2068
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 2 cut(s) 437, 1101
AhdI GACNNNNNGTC 1 cut(s) 2092
AjnI CCWGG 3 cut(s) 1378, 1447, 2051
AluBI AGCT 5 cut(s) 641, 781, 1334, 1610, 1637
AluI AGCT 5 cut(s) 641, 781, 1334, 1610, 1637
Alw21I GWGCWC 1 cut(s) 1387
Alw26I GTCTC 5 cut(s) 272, 1782, 1941, 1968, 1998
AlwI GGATC 7 cut(s) 39, 878, 1053, 1066, 1322, 1747, 1779
Ama87I CYCGRG 1 cut(s) 1908
AoxI GGCC 7 cut(s) 66, 84, 398, 900, 1455, 1538, 1688
ApaI GGGCCC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 526, 599, 1637
AsiGI ACCGGT 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 827
AspLEI GCGC 2 cut(s) 942, 1795
AsuC2I CCSGG 3 cut(s) 264, 693, 1072
AsuHPI GGTGA 7 cut(s) 213, 284, 320, 761, 957, 1598, 1670
AvaI CYCGRG 1 cut(s) 1908
AvaII GGWCC 8 cut(s) 109, 593, 689, 806, 1193, 1202, 1343, 1557
BaeGI GKGCMC 1 cut(s) 402
BalI TGGCCA 1 cut(s) 1690
BamHI GGATCC 1 cut(s) 1058
BanI GGYRCC 2 cut(s) 710, 939
BanII GRGCYC 1 cut(s) 402
BarI GAAGNNNNNNTAC 1 cut(s) 2119
BbrPI CACGTG 1 cut(s) 2069
BbsI GAAGAC 2 cut(s) 1407, 1974
Bbv12I GWGCWC 1 cut(s) 1387
BbvI GCAGC 3 cut(s) 538, 586, 1624
BccI CCATC 6 cut(s) 68, 305, 1306, 1529, 1572, 1656
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 1619, 1653
BciT130I CCWGG 3 cut(s) 1380, 1449, 2053
BclI TGATCA 2 cut(s) 22, 1987
BcnI CCSGG 3 cut(s) 264, 693, 1072
BcoDI GTCTC 5 cut(s) 272, 1782, 1941, 1968, 1998
BfaI CTAG 4 cut(s) 350, 1566, 1620, 1797
BfmI CTRYAG 1 cut(s) 1029
BfoI RGCGCY 1 cut(s) 943
BfuAI ACCTGC 1 cut(s) 1006
BisI GCNGC 6 cut(s) 527, 530, 600, 714, 1638, 2132
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 6 cut(s) 528, 531, 601, 715, 1639, 2133
Bme1390I CCNGG 6 cut(s) 264, 693, 1072, 1380, 1449, 2053
Bme18I GGWCC 8 cut(s) 109, 593, 689, 806, 1193, 1202, 1343, 1557
BmeRI GACNNNNNGTC 1 cut(s) 2092
BmeT110I CYCGRG 1 cut(s) 1908
BmiI GGNNCC 7 cut(s) 399, 400, 712, 941, 1010, 1060, 1458
BmrFI CCNGG 6 cut(s) 264, 693, 1072, 1380, 1449, 2053
BmrI ACTGGG 1 cut(s) 1165
BmsI GCATC 7 cut(s) 458, 763, 906, 971, 1284, 1506, 2098
BmuI ACTGGG 1 cut(s) 1165
BpiI GAAGAC 2 cut(s) 1407, 1974
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BpmI CTGGAG 2 cut(s) 803, 1470
Bpu1102I GCTNAGC 1 cut(s) 522
BpuEI CTTGAG 3 cut(s) 231, 981, 2061
BpuMI CCSGG 3 cut(s) 264, 693, 1072
BsaAI YACGTR 1 cut(s) 2069
BsaBI GATNNNNATC 3 cut(s) 1770, 1917, 1935
BsaHI GRCGYC 2 cut(s) 123, 940
BsaI GGTCTC 3 cut(s) 272, 1941, 1998
BsaJI CCNNGG 3 cut(s) 87, 897, 1205
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 776, 806
Bse118I RCCGGY 2 cut(s) 281, 1103
Bse1I ACTGG 4 cut(s) 1087, 1160, 1196, 1736
Bse8I GATNNNNATC 3 cut(s) 1770, 1917, 1935
BseBI CCWGG 3 cut(s) 1380, 1449, 2053
BseDI CCNNGG 3 cut(s) 87, 897, 1205
BseGI GGATG 9 cut(s) 10, 175, 568, 921, 1583, 1768, 2048, 2089, 2113
BseJI GATNNNNATC 3 cut(s) 1770, 1917, 1935
BseMII CTCAG 5 cut(s) 536, 1187, 1316, 1416, 2133
BseNI ACTGG 4 cut(s) 1087, 1160, 1196, 1736
BseRI GAGGAG 7 cut(s) 104, 212, 290, 432, 711, 1066, 1692
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 3 cut(s) 538, 586, 1624
BseYI CCCAGC 1 cut(s) 1610
Bsh1236I CGCG 1 cut(s) 1793
BshFI GGCC 7 cut(s) 68, 86, 400, 902, 1457, 1540, 1690
BshNI GGYRCC 2 cut(s) 710, 939
BshTI ACCGGT 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 1387
BsiHKCI CYCGRG 1 cut(s) 1908
BsiSI CCGG 5 cut(s) 264, 282, 692, 1071, 1104
BslFI GGGAC 4 cut(s) 745, 1742, 1889, 2055
BsmAI GTCTC 5 cut(s) 272, 1782, 1941, 1968, 1998
BsmBI CGTCTC 1 cut(s) 1782
BsmFI GGGAC 4 cut(s) 745, 1742, 1889, 2055
BsmI GAATGC 2 cut(s) 1475, 1497
BsnI GGCC 7 cut(s) 68, 86, 400, 902, 1457, 1540, 1690
Bso31I GGTCTC 3 cut(s) 272, 1941, 1998
BsoBI CYCGRG 1 cut(s) 1908
Bsp120I GGGCCC 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 402, 1387
Bsp1720I GCTNAGC 1 cut(s) 522
BspACI CCGC 9 cut(s) 43, 62, 411, 530, 572, 714, 728, 1107, 2131
BspANI GGCC 7 cut(s) 68, 86, 400, 902, 1457, 1540, 1690
BspCNI CTCAG 5 cut(s) 535, 1186, 1315, 1415, 2134
BspFNI CGCG 1 cut(s) 1793
BspHI TCATGA 3 cut(s) 1669, 1816, 1915
BspLI GGNNCC 7 cut(s) 399, 400, 712, 941, 1010, 1060, 1458
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 7 cut(s) 39, 878, 1053, 1066, 1322, 1747, 1779
BspQI GCTCTTC 1 cut(s) 1584
BspT107I GGYRCC 2 cut(s) 710, 939
BspTNI GGTCTC 3 cut(s) 272, 1941, 1998
BsrBI CCGCTC 1 cut(s) 728
BsrFI RCCGGY 2 cut(s) 281, 1103
BsrI ACTGG 4 cut(s) 1087, 1160, 1196, 1736
BssAI RCCGGY 2 cut(s) 281, 1103
BssECI CCNNGG 3 cut(s) 87, 897, 1205
BssNI GRCGYC 2 cut(s) 123, 940
BssT1I CCWWGG 1 cut(s) 1205
Bst2UI CCWGG 3 cut(s) 1380, 1449, 2053
Bst4CI ACNGT 6 cut(s) 585, 866, 1952, 2009, 2046, 2090
Bst6I CTCTTC 3 cut(s) 1480, 1584, 2117
BstACI GRCGYC 2 cut(s) 123, 940
BstBAI YACGTR 1 cut(s) 2069
BstC8I GCNNGC 3 cut(s) 447, 639, 904
BstDEI CTNAG 5 cut(s) 522, 1173, 1302, 1402, 2142
BstDSI CCRYGG 1 cut(s) 897
BstEII GGTNACC 1 cut(s) 1018
BstENI CCTNNNNNAGG 2 cut(s) 1401, 1524
BstF5I GGATG 9 cut(s) 10, 175, 568, 921, 1583, 1768, 2048, 2089, 2113
BstFNI CGCG 1 cut(s) 1793
BstH2I RGCGCY 1 cut(s) 943
BstHHI GCGC 2 cut(s) 942, 1795
BstMAI GTCTC 5 cut(s) 272, 1782, 1941, 1968, 1998
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 3 cut(s) 1380, 1449, 2053
BstNSI RCATGY 1 cut(s) 631
BstPI GGTNACC 1 cut(s) 1018
BstSCI CCNGG 6 cut(s) 262, 691, 1070, 1378, 1447, 2051
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 1793
BstV1I GCAGC 3 cut(s) 538, 586, 1624
BstV2I GAAGAC 2 cut(s) 1407, 1974
BstX2I RGATCY 2 cut(s) 1058, 1739
BstXI CCANNNNNNTGG 1 cut(s) 1655
BstYI RGATCY 2 cut(s) 1058, 1739
BsuRI GGCC 7 cut(s) 68, 86, 400, 902, 1457, 1540, 1690
BtgI CCRYGG 1 cut(s) 897
BtgZI GCGATG 1 cut(s) 1235
BtsCI GGATG 9 cut(s) 10, 175, 568, 921, 1583, 1768, 2048, 2089, 2113
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 1 cut(s) 1038
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 3 cut(s) 447, 639, 904
CciI TCATGA 3 cut(s) 1669, 1816, 1915
CfoI GCGC 2 cut(s) 942, 1795
Cfr10I RCCGGY 2 cut(s) 281, 1103
CseI GACGC 1 cut(s) 1799
Csp6I GTAC 2 cut(s) 862, 1999
CspAI ACCGGT 1 cut(s) 281
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviQI GTAC 2 cut(s) 862, 1999
DdeI CTNAG 5 cut(s) 522, 1173, 1302, 1402, 2142
DinI GGCGCC 1 cut(s) 941
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 2092
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 3 cut(s) 66, 84, 1688
Eam1104I CTCTTC 3 cut(s) 1480, 1584, 2117
Eam1105I GACNNNNNGTC 1 cut(s) 2092
EarI CTCTTC 3 cut(s) 1480, 1584, 2117
Eco130I CCWWGG 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 3 cut(s) 272, 1941, 1998
Eco32I GATATC 1 cut(s) 1757
Eco47I GGWCC 8 cut(s) 109, 593, 689, 806, 1193, 1202, 1343, 1557
Eco72I CACGTG 1 cut(s) 2069
Eco88I CYCGRG 1 cut(s) 1908
Eco91I GGTNACC 1 cut(s) 1018
EcoNI CCTNNNNNAGG 2 cut(s) 1401, 1524
EcoO109I RGGNCCY 2 cut(s) 109, 398
EcoO65I GGTNACC 1 cut(s) 1018
EcoRII CCWGG 3 cut(s) 1378, 1447, 2051
EcoRV GATATC 1 cut(s) 1757
EcoT14I CCWWGG 1 cut(s) 1205
EcoT22I ATGCAT 1 cut(s) 921
EcoT38I GRGCYC 1 cut(s) 402
EgeI GGCGCC 1 cut(s) 941
EheI GGCGCC 1 cut(s) 941
ErhI CCWWGG 1 cut(s) 1205
Esp3I CGTCTC 1 cut(s) 1782
FaqI GGGAC 4 cut(s) 745, 1742, 1889, 2055
FauI CCCGC 3 cut(s) 404, 721, 1100
FbaI TGATCA 2 cut(s) 22, 1987
Fnu4HI GCNGC 6 cut(s) 527, 530, 600, 714, 1638, 2132
FokI GGATG 9 cut(s) 17, 182, 555, 928, 1590, 1775, 2035, 2096, 2120
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 6 cut(s) 527, 530, 600, 714, 1638, 2132
FspBI CTAG 4 cut(s) 350, 1566, 1620, 1797
GlaI GCGC 2 cut(s) 941, 1794
GluI GCNGC 6 cut(s) 527, 530, 600, 714, 1638, 2132
GsaI CCCAGC 1 cut(s) 1614
GsuI CTGGAG 2 cut(s) 803, 1470
HaeII RGCGCY 1 cut(s) 943
HaeIII GGCC 7 cut(s) 68, 86, 400, 902, 1457, 1540, 1690
HapII CCGG 5 cut(s) 264, 282, 692, 1071, 1104
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 2 cut(s) 942, 1795
Hin1I GRCGYC 2 cut(s) 123, 940
Hin6I GCGC 2 cut(s) 940, 1793
HinP1I GCGC 2 cut(s) 940, 1793
HinfI GANTC 6 cut(s) 218, 242, 346, 1407, 1468, 1616
HpaII CCGG 5 cut(s) 264, 282, 692, 1071, 1104
HphI GGTGA 7 cut(s) 213, 284, 320, 761, 957, 1598, 1670
Hpy166II GTNNAC 4 cut(s) 765, 1343, 1925, 1943
Hpy188I TCNGA 9 cut(s) 241, 258, 345, 1561, 1645, 1723, 1747, 1834, 2143
Hpy8I GTNNAC 4 cut(s) 765, 1343, 1925, 1943
Hpy99I CGWCG 6 cut(s) 17, 74, 101, 125, 241, 2094
HpyAV CCTTC 5 cut(s) 905, 1224, 1696, 1833, 2121
HpyCH4III ACNGT 6 cut(s) 585, 866, 1952, 2009, 2046, 2090
HpyCH4IV ACGT 4 cut(s) 123, 1903, 1927, 2068
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 5 cut(s) 522, 1173, 1302, 1402, 2142
HpySE526I ACGT 4 cut(s) 123, 1903, 1927, 2068
Hsp92I GRCGYC 2 cut(s) 123, 940
HspAI GCGC 2 cut(s) 940, 1793
KasI GGCGCC 1 cut(s) 939
Ksp22I TGATCA 2 cut(s) 22, 1987
LguI GCTCTTC 1 cut(s) 1584
LmnI GCTCC 3 cut(s) 238, 725, 1390
Lsp1109I GCAGC 3 cut(s) 538, 586, 1624
LweI GCATC 7 cut(s) 458, 763, 906, 971, 1284, 1506, 2098
MaeI CTAG 4 cut(s) 350, 1566, 1620, 1797
MaeII ACGT 4 cut(s) 123, 1903, 1927, 2068
MaeIII GTNAC 8 cut(s) 219, 585, 659, 1018, 1091, 1151, 1952, 2071
MbiI CCGCTC 1 cut(s) 728
MboII GAAGA 9 cut(s) 214, 472, 1407, 1497, 1601, 1734, 1764, 1979, 2134
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 2 cut(s) 1058, 1739
MhlI GDGCHC 2 cut(s) 402, 1387
MlsI TGGCCA 1 cut(s) 1690
MluCI AATT 5 cut(s) 437, 539, 889, 1041, 1111
MluNI TGGCCA 1 cut(s) 1690
Mly113I GGCGCC 1 cut(s) 940
MlyI GAGTC 2 cut(s) 227, 1625
MmeI TCCRAC 4 cut(s) 219, 631, 1180, 1584
Mox20I TGGCCA 1 cut(s) 1690
Mph1103I ATGCAT 1 cut(s) 921
MroXI GAANNNNTTC 1 cut(s) 827
MscI TGGCCA 1 cut(s) 1690
MseI TTAA 3 cut(s) 813, 933, 978
MslI CAYNNNNRTG 6 cut(s) 924, 1599, 1653, 1821, 1920, 2025
Msp20I TGGCCA 1 cut(s) 1690
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 5 cut(s) 264, 282, 692, 1071, 1104
MspR9I CCNGG 6 cut(s) 264, 693, 1072, 1380, 1449, 2053
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 2 cut(s) 1475, 1497
MvaI CCWGG 3 cut(s) 1380, 1449, 2053
MvnI CGCG 1 cut(s) 1793
MwoI GCNNNNNNNGC 1 cut(s) 59
NarI GGCGCC 1 cut(s) 940
NciI CCSGG 3 cut(s) 264, 693, 1072
NlaIV GGNNCC 7 cut(s) 399, 400, 712, 941, 1010, 1060, 1458
NmeAIII GCCGAG 2 cut(s) 38, 112
NmuCI GTSAC 2 cut(s) 219, 1952
NsiI ATGCAT 1 cut(s) 921
NspI RCATGY 1 cut(s) 631
PaeR7I CTCGAG 1 cut(s) 1908
PagI TCATGA 3 cut(s) 1669, 1816, 1915
PciSI GCTCTTC 1 cut(s) 1584
PctI GAATGC 2 cut(s) 1475, 1497
PdmI GAANNNNTTC 1 cut(s) 827
PfeI GAWTC 4 cut(s) 242, 346, 1407, 1468
PflMI CCANNNNNTGG 1 cut(s) 1337
PfoI TCCNGGA 1 cut(s) 691
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 6 cut(s) 528, 531, 601, 715, 1639, 2133
PleI GAGTC 2 cut(s) 226, 1624
PluTI GGCGCC 1 cut(s) 943
PmaCI CACGTG 1 cut(s) 2069
PmlI CACGTG 1 cut(s) 2069
PpsI GAGTC 2 cut(s) 226, 1624
Ppu21I YACGTR 1 cut(s) 2069
PpuMI RGGWCCY 1 cut(s) 109
Psp1406I AACGTT 1 cut(s) 1903
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 3 cut(s) 1378, 1447, 2051
PspCI CACGTG 1 cut(s) 2069
PspEI GGTNACC 1 cut(s) 1018
PspFI CCCAGC 1 cut(s) 1610
PspGI CCWGG 3 cut(s) 1378, 1447, 2051
PspN4I GGNNCC 7 cut(s) 399, 400, 712, 941, 1010, 1060, 1458
PspOMI GGGCCC 1 cut(s) 398
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 1033
PsuI RGATCY 2 cut(s) 1058, 1739
RsaI GTAC 2 cut(s) 863, 2000
RsaNI GTAC 2 cut(s) 862, 1999
RseI CAYNNNNRTG 6 cut(s) 924, 1599, 1653, 1821, 1920, 2025
SapI GCTCTTC 1 cut(s) 1584
SaqAI TTAA 3 cut(s) 813, 933, 978
SatI GCNGC 6 cut(s) 527, 530, 600, 714, 1638, 2132
SchI GAGTC 2 cut(s) 227, 1625
ScrFI CCNGG 6 cut(s) 264, 693, 1072, 1380, 1449, 2053
SduI GDGCHC 2 cut(s) 402, 1387
SfaNI GCATC 7 cut(s) 458, 763, 906, 971, 1284, 1506, 2098
SfcI CTRYAG 1 cut(s) 1029
SfoI GGCGCC 1 cut(s) 941
Sfr274I CTCGAG 1 cut(s) 1908
SinI GGWCC 8 cut(s) 109, 593, 689, 806, 1193, 1202, 1343, 1557
SlaI CTCGAG 1 cut(s) 1908
SmiMI CAYNNNNRTG 6 cut(s) 924, 1599, 1653, 1821, 1920, 2025
SmlI CTYRAG 4 cut(s) 246, 996, 1908, 2076
SmoI CTYRAG 4 cut(s) 246, 996, 1908, 2076
Sse9I AATT 5 cut(s) 437, 539, 889, 1041, 1111
SsiI CCGC 9 cut(s) 43, 62, 411, 530, 572, 714, 728, 1107, 2131
SspDI GGCGCC 1 cut(s) 939
SspI AATATT 1 cut(s) 738
SspMI CTAG 4 cut(s) 350, 1566, 1620, 1797
StyD4I CCNGG 6 cut(s) 262, 691, 1070, 1378, 1447, 2051
StyI CCWWGG 1 cut(s) 1205
TaaI ACNGT 6 cut(s) 585, 866, 1952, 2009, 2046, 2090
TaiI ACGT 4 cut(s) 126, 1906, 1930, 2071
TaqI TCGA 5 cut(s) 452, 831, 1867, 1897, 1909
TaqII GACCGA 1 cut(s) 2082
TasI AATT 5 cut(s) 437, 539, 889, 1041, 1111
TatI WGTACW 1 cut(s) 1998
TauI GCSGC 3 cut(s) 532, 716, 2134
TfiI GAWTC 4 cut(s) 242, 346, 1407, 1468
Tru1I TTAA 3 cut(s) 813, 933, 978
Tru9I TTAA 3 cut(s) 813, 933, 978
TscAI CASTG 1 cut(s) 1038
TseFI GTSAC 2 cut(s) 219, 1952
TseI GCWGC 3 cut(s) 526, 599, 1637
Tsp45I GTSAC 2 cut(s) 219, 1952
TspDTI ATGAA 9 cut(s) 21, 392, 680, 1080, 1238, 1407, 1514, 1715, 2043
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 1337
VpaK11BI GGWCC 8 cut(s) 109, 593, 689, 806, 1193, 1202, 1343, 1557
XagI CCTNNNNNAGG 2 cut(s) 1401, 1524
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 1504
XhoI CTCGAG 1 cut(s) 1908
XmnI GAANNNNTTC 1 cut(s) 827
XspI CTAG 4 cut(s) 350, 1566, 1620, 1797
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.