Rorug05G0281700

cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006731
Physical Location & Seq
Reverse (-)
32176787 .. 32185444
8658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0281700.1

Sequence Viewer

Length: 2418 bp
ATGGCCGCCACTGCTTCTCAACATGCTTTTGAGGGAATATTAAACAGTCTCCCAAAGCCTGGAGGTGGCGAATTCGGAAAATTTTACAGTCTACCTGCTCTAAATGATCCAAGAATTGACAGGCTTCCATATTCAATAAAGATACTTTTGGAATCTGCAATCCGTAACTGTGACAACTTCCAAATTACCAAGGAAGATGTTGAAAAGATAGCTGATTGGGAGAATACATCACCAAAGCAAGTTGAAATACCCTTTAAGCCTGCTCTTGTTCTCCTCCAGGACTTCACTGGAGTACCAGCTGTGATTGACCTTGCACGTATCCGGGATGCCATGGAGGAGCTTGGAAATGATCCCAACAAGATAAATCCATTGGTTCCTGTAGACTTTGTTGTTGATCATTCGGTTACAGTTGATGTAGCAAGAACTGAAGATGCACTACAGGAAAATATGGAACGAGAGTTTCAGAGAAATAAAGAGAGATTTGCTTTCCTTAAATGGGGATCTTTTGCATTCCATAACATGCTTGTAGTACCTCCTGGTTCTGGAATTGTCCATCAGGTTAATCTGGAATACCTTGCCAGGGTTGTTTTCGAAACTGATGGAATTCTATACCCTGATAGCGTGGTTGGTACCGATTCCCACACAACCATGATTGATGGGAAAGGAGTTGCTGGCTGGGGAGTGGGTGGAATTGAAGCTGAGGCAACAATGCTTGGTCAGCTTACTGGGCAACTGCGTGAAGGTGTCACTGCTACCGACTTAGTTTTAACTGTGACACAAATGTTGAAGAAACATGGTGTTGTTGGCAAGTTTGTCGAGTTCTATGGTGAGGGCATGGGTGAAATATCACTCGCTGACAGGGCCACAATTGCAAACATGTCTCCTGAATATGGAGCAACCATGGGCTTCTTTCCTGTAGATCATGTTACATTGCAGTATCTTAAACTGACTGGAAGAAGTGATGAGAAGGTTGCAATGATAGAATCATACTTGAGGGCAAACAAAATGTTCATCGACTACAATGAGGGTTTTGCTGTGCCAAAAGAGAAACAAGGTGAAGTTGCAAAGTTTTTGTTTCATGGACAACCTGCTGAACTTACGCATGGCAGCATTGTCATTGCAGCAGTTACAAGTTGTACAAACACATCTAATCCCACTGTAATGCTTGGGGCTGGACTAGTTGCGAAGAAAGCTTGTGAACTAGGCTTGGAGGTTAAGCCATGGATAAAAACAAGTCTTGCTCCTGGTTCTGGCGTTGTAACCAAATATCTAATTCAGAGTGGCCTGCAGAAGTACTTAAACCAGCAGGGTTTTAATGTTGTTGGCTTCGGCTGCACTACATGTATTGGGAACTCTGGTGAACTTGATGAATCAGTTGCTTCTGCAATCTCAGATAATGACATCATTGCTGCAGCTGTTCTTTCTGGAAACCGGAACTTTGAAGGTCATATTCACCCGTTAACAAGAGCTAACTATCTTGCTTCGCCTCCATTGGTTGACATTAATTTCGATCAAGAGCCAATTGGAAAAGGGAAGGATGGGAAGAGTGTGTCCTTGAAGGATATCTGGCCTAGTAATGATGAAATTGCACAGGTATTTCAATCTCTTGTCTTACCTGATATGTTTAAGAGCACATATGAGGCCATTACAAAGGGTAACCATTTGTGGAATGAGCTCTCTGTCCCTTATTCGACTCTTTATTCTTGGGATGCAAACTCGACCTACATTCACAAGCCCCCATTTTTAAAGAACATGAGCATGGAACTACCAGGTCCTCGTGGCGTAAAGGATGCATATTGTTTGCTGTATTTAGGCGACAGTATAACCACAGATCATATTTCTCCTGGAGGTACCATCCACAAAGACAGCCTTGCTGCACAGTATCTCCGTGAGCTTGGGGTGGATCGCAAAGATTTCAATTCTTATGGTTGTCGACGGGGAAATGATGAGGTAATGGTGCGGGGTACCTTTGCCAATATCCGCATTCTGAACAAACTATTGAATGGGGAAGTCGGTCCAAAAACAATTCACATTCCAACCGGGGAGAAACTTCATGTGTATGAAGCAGCAATGAGGTACAAGGCTGCTGATCAGGAAACTGTTGTCTTAGCTGGAGCAGAGTATGGATGTGGCAGCGCTCGAGATTGGGCTGCCAAGGGTCCAATGCTACTAGGAGTGAGAGCAGTTATAACCAAAAGCTTTGAAAGCATTCATCGTAGCAATCTGGTAGGGATGGGAATCATTCCCTTATGTTTCAAGCCAGGTGAGGATGCAGAAACCCTCGGATTGACTGGTCATGAGCGCTACACGATAGACCTTCCTAGTAAAGTTAGTGAGATAAGGCCTGGCCAGGATGTCAAGGTCACAACTGATACAGGCAAATCCTTCACATGCACACTCCGTTTTGACACAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000381 GO:0000785 GO:0000790 GO:0000791 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005694 GO:0005719 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006508 GO:0006509 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022607 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0031982 GO:0032774 GO:0032991 GO:0033119 GO:0033120 GO:0033619 GO:0034097 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043230 GO:0043231 GO:0043232 GO:0043233 GO:0043484 GO:0043565 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044421 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044530 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048024 GO:0048025 GO:0048026 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050685 GO:0050686 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051259 GO:0051260 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070062 GO:0070555 GO:0070887 GO:0071013 GO:0071310 GO:0071345 GO:0071347 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902680 GO:1903311 GO:1903312 GO:1903313 GO:1903506 GO:1903508 GO:1903561 GO:1990837 GO:1990904 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
RRM_1

Protein Analysis

805

Amino Acids

87.9

Weight (kDa)

5.5

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 68 - 337 7.6e-86 Aconitase family (aconitate hydratase)
Aconitase PF00330 360 - 500 1.5e-43 Aconitase family (aconitate hydratase)
Aconitase_C PF00694 626 - 753 2.7e-39 Aconitase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2189
Acc36I ACCTGC 2 cut(s) 103, 1096
Acc65I GGTACC 3 cut(s) 629, 1850, 1964
AccB1I GGYRCC 3 cut(s) 629, 1850, 1964
AccB7I CCANNNNNTGG 1 cut(s) 59
AccI GTMKAC 3 cut(s) 91, 381, 1933
AciI CCGC 3 cut(s) 6, 1960, 1981
AclWI GGATC 4 cut(s) 101, 344, 508, 1911
AcoI YGGCCR 2 cut(s) 3, 2347
AcsI RAATTY 3 cut(s) 71, 80, 603
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 8 cut(s) 294, 531, 631, 1138, 1295, 1852, 1966, 2078
AfeI AGCGCT 2 cut(s) 2137, 2303
AfiI CCNNNNNNNGG 7 cut(s) 59, 65, 496, 542, 580, 890, 1250
AflIII ACRYGT 2 cut(s) 876, 1340
AhlI ACTAGT 1 cut(s) 1177
Alw21I GWGCWC 2 cut(s) 1634, 1677
Alw26I GTCTC 2 cut(s) 53, 885
AlwI GGATC 4 cut(s) 101, 344, 508, 1911
Ama87I CYCGRG 1 cut(s) 2139
Aor51HI AGCGCT 2 cut(s) 2137, 2303
AoxI GGCC 7 cut(s) 3, 861, 1282, 1568, 1641, 2342, 2347
ApoI RAATTY 3 cut(s) 71, 80, 603
ArsI GACNNNNNNTTYG 2 cut(s) 1490, 1522
AseI ATTAAT 1 cut(s) 1503
Asp700I GAANNNNTTC 1 cut(s) 2208
Asp718I GGTACC 3 cut(s) 629, 1850, 1964
AspLEI GCGC 2 cut(s) 2138, 2304
AspS9I GGNCC 4 cut(s) 861, 1772, 2015, 2159
AsuC2I CCSGG 2 cut(s) 323, 2041
AsuHPI GGTGA 7 cut(s) 222, 839, 851, 1067, 1370, 1445, 2276
AsuII TTCGAA 1 cut(s) 591
AvaI CYCGRG 1 cut(s) 2139
AvaII GGWCC 3 cut(s) 1772, 2015, 2159
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BalI TGGCCA 1 cut(s) 2349
BanI GGYRCC 3 cut(s) 629, 1850, 1964
BanII GRGCYC 1 cut(s) 1677
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 1776
Bbv12I GWGCWC 2 cut(s) 1634, 1677
BbvCI CCTCAGC 1 cut(s) 699
BccI CCATC 6 cut(s) 561, 593, 650, 1532, 1862, 2227
BcgI CGANNNNNNTGC 2 cut(s) 796, 830
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 2 cut(s) 394, 2089
BcnI CCSGG 2 cut(s) 323, 2041
BcoDI GTCTC 2 cut(s) 53, 885
BcuI ACTAGT 1 cut(s) 1177
BfaI CTAG 5 cut(s) 1178, 1202, 1572, 2171, 2322
BfmI CTRYAG 5 cut(s) 378, 437, 915, 1286, 1410
BfoI RGCGCY 2 cut(s) 2139, 2305
BfuAI ACCTGC 2 cut(s) 103, 1096
BfuI GTATCC 1 cut(s) 329
BmcAI AGTACT 1 cut(s) 1295
Bme18I GGWCC 3 cut(s) 1772, 2015, 2159
BmeT110I CYCGRG 1 cut(s) 2139
BmgT120I GGNCC 4 cut(s) 861, 1772, 2015, 2159
BmiI GGNNCC 5 cut(s) 375, 631, 1852, 1966, 2160
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 5 cut(s) 316, 421, 1699, 1780, 2260
BmuI ACTGGG 1 cut(s) 735
BpmI CTGGAG 5 cut(s) 81, 260, 309, 1866, 2133
Bpu10I CCTNAGC 1 cut(s) 699
Bpu14I TTCGAA 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 1012
BpuMI CCSGG 2 cut(s) 323, 2041
BsaAI YACGTR 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 2237
BsaJI CCNNGG 8 cut(s) 189, 330, 579, 900, 1220, 2040, 2154, 2281
BsaWI WCCGGW 1 cut(s) 1431
BsaXI ACNNNNNCTCC 2 cut(s) 1869, 1899
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 65, 496, 542, 580, 890, 1250
Bse1I ACTGG 4 cut(s) 292, 730, 955, 2296
Bse3DI GCAATG 5 cut(s) 929, 981, 1116, 1404, 2076
Bse8I GATNNNNATC 1 cut(s) 2237
BseDI CCNNGG 8 cut(s) 189, 330, 579, 900, 1220, 2040, 2154, 2281
BseGI GGATG 9 cut(s) 331, 1543, 1714, 1795, 1854, 2132, 2238, 2275, 2359
BseJI GATNNNNATC 1 cut(s) 2237
BseLI CCNNNNNNNGG 7 cut(s) 59, 65, 496, 542, 580, 890, 1250
BseMI GCAATG 5 cut(s) 929, 981, 1116, 1404, 2076
BseMII CTCAG 2 cut(s) 690, 1404
BseNI ACTGG 4 cut(s) 292, 730, 955, 2296
BseRI GAGGAG 2 cut(s) 263, 350
BseYI CCCAGC 1 cut(s) 675
BsgI GTGCAG 2 cut(s) 1318, 1860
BshFI GGCC 7 cut(s) 5, 863, 1284, 1570, 1643, 2344, 2349
BshNI GGYRCC 3 cut(s) 629, 1850, 1964
BsiHKAI GWGCWC 2 cut(s) 1634, 1677
BsiHKCI CYCGRG 1 cut(s) 2139
BsiSI CCGG 3 cut(s) 322, 1432, 2040
BslFI GGGAC 1 cut(s) 1667
BslI CCNNNNNNNGG 7 cut(s) 59, 65, 496, 542, 580, 890, 1250
BsmAI GTCTC 2 cut(s) 53, 885
BsmFI GGGAC 1 cut(s) 1667
BsmI GAATGC 3 cut(s) 509, 1983, 2208
BsnI GGCC 7 cut(s) 5, 863, 1284, 1570, 1643, 2344, 2349
BsoBI CYCGRG 1 cut(s) 2139
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 1634, 1677
Bsp1407I TGTACA 1 cut(s) 1136
Bsp143I GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
Bsp19I CCATGG 3 cut(s) 330, 900, 1220
BspACI CCGC 3 cut(s) 6, 1960, 1981
BspANI GGCC 7 cut(s) 5, 863, 1284, 1570, 1643, 2344, 2349
BspCNI CTCAG 2 cut(s) 691, 1403
BspHI TCATGA 1 cut(s) 2296
BspLI GGNNCC 5 cut(s) 375, 631, 1852, 1966, 2160
BspMAI CTGCAG 2 cut(s) 1290, 1414
BspMI ACCTGC 2 cut(s) 103, 1096
BspPI GGATC 4 cut(s) 101, 344, 508, 1911
BspT104I TTCGAA 1 cut(s) 591
BspT107I GGYRCC 3 cut(s) 629, 1850, 1964
BsrDI GCAATG 5 cut(s) 929, 981, 1116, 1404, 2076
BsrGI TGTACA 1 cut(s) 1136
BsrI ACTGG 4 cut(s) 292, 730, 955, 2296
BssECI CCNNGG 8 cut(s) 189, 330, 579, 900, 1220, 2040, 2154, 2281
BssMI GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
BssSI CACGAG 1 cut(s) 1776
BssT1I CCWWGG 5 cut(s) 189, 330, 900, 1220, 2154
Bst2BI CACGAG 1 cut(s) 1776
Bst4CI ACNGT 9 cut(s) 47, 89, 170, 409, 772, 1159, 1820, 1881, 2101
Bst6I CTCTTC 1 cut(s) 1538
BstAUI TGTACA 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 317
BstBI TTCGAA 1 cut(s) 591
BstC8I GCNNGC 3 cut(s) 261, 673, 1286
BstDEI CTNAG 4 cut(s) 699, 760, 1390, 2107
BstDSI CCRYGG 3 cut(s) 330, 900, 1220
BstEII GGTNACC 1 cut(s) 1655
BstF5I GGATG 9 cut(s) 331, 1543, 1714, 1795, 1854, 2132, 2238, 2275, 2359
BstH2I RGCGCY 2 cut(s) 2139, 2305
BstHHI GCGC 2 cut(s) 2138, 2304
BstKTI GATC 9 cut(s) 109, 352, 397, 503, 922, 1513, 1834, 1906, 2092
BstMAI GTCTC 2 cut(s) 53, 885
BstMBI GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
BstMWI GCNNNNNNNGC 8 cut(s) 11, 718, 727, 860, 869, 1190, 1332, 2205
BstNSI RCATGY 5 cut(s) 26, 523, 880, 1344, 2394
BstPI GGTNACC 1 cut(s) 1655
BstSFI CTRYAG 5 cut(s) 378, 437, 915, 1286, 1410
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 7 cut(s) 5, 863, 1284, 1570, 1643, 2344, 2349
BtgI CCRYGG 3 cut(s) 330, 900, 1220
BtsCI GGATG 9 cut(s) 331, 1543, 1714, 1795, 1854, 2132, 2238, 2275, 2359
BtsI GCAGTG 2 cut(s) 9, 747
BtsIMutI CAGTG 4 cut(s) 9, 285, 747, 1155
BveI ACCTGC 2 cut(s) 103, 1096
Cac8I GCNNGC 3 cut(s) 261, 673, 1286
CciI TCATGA 1 cut(s) 2296
CfoI GCGC 2 cut(s) 2138, 2304
Cfr13I GGNCC 4 cut(s) 861, 1772, 2015, 2159
CsiI ACCWGGT 1 cut(s) 1768
Csp6I GTAC 8 cut(s) 293, 530, 630, 1137, 1294, 1851, 1965, 2077
CviQI GTAC 8 cut(s) 293, 530, 630, 1137, 1294, 1851, 1965, 2077
DdeI CTNAG 4 cut(s) 699, 760, 1390, 2107
DpnI GATC 9 cut(s) 108, 351, 396, 502, 921, 1512, 1833, 1905, 2091
DpnII GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
DraI TTTAAA 1 cut(s) 1746
EaeI YGGCCR 2 cut(s) 3, 2347
Eam1104I CTCTTC 1 cut(s) 1538
EarI CTCTTC 1 cut(s) 1538
Ecl136II GAGCTC 1 cut(s) 1675
Eco130I CCWWGG 5 cut(s) 189, 330, 900, 1220, 2154
Eco147I AGGCCT 1 cut(s) 2344
Eco24I GRGCYC 1 cut(s) 1677
Eco32I GATATC 1 cut(s) 1564
Eco47I GGWCC 3 cut(s) 1772, 2015, 2159
Eco47III AGCGCT 2 cut(s) 2137, 2303
Eco53kI GAGCTC 1 cut(s) 1675
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 2139
Eco91I GGTNACC 1 cut(s) 1655
EcoICRI GAGCTC 1 cut(s) 1675
EcoO109I RGGNCCY 1 cut(s) 1772
EcoO65I GGTNACC 1 cut(s) 1655
EcoRI GAATTC 2 cut(s) 71, 603
EcoRV GATATC 1 cut(s) 1564
EcoT14I CCWWGG 5 cut(s) 189, 330, 900, 1220, 2154
EcoT22I ATGCAT 1 cut(s) 1795
EcoT38I GRGCYC 1 cut(s) 1677
ErhI CCWWGG 5 cut(s) 189, 330, 900, 1220, 2154
FalI AAGNNNNNCTT 2 cut(s) 1854, 1886
FaqI GGGAC 1 cut(s) 1667
FauI CCCGC 1 cut(s) 1953
FauNDI CATATG 1 cut(s) 1636
FbaI TGATCA 2 cut(s) 394, 2089
FblI GTMKAC 3 cut(s) 91, 381, 1933
FokI GGATG 9 cut(s) 338, 1550, 1721, 1802, 1841, 2139, 2245, 2282, 2366
FriOI GRGCYC 1 cut(s) 1677
FspBI CTAG 5 cut(s) 1178, 1202, 1572, 2171, 2322
GlaI GCGC 2 cut(s) 2137, 2303
GsaI CCCAGC 1 cut(s) 679
GsuI CTGGAG 5 cut(s) 81, 260, 309, 1866, 2133
HaeII RGCGCY 2 cut(s) 2139, 2305
HaeIII GGCC 7 cut(s) 5, 863, 1284, 1570, 1643, 2344, 2349
HapII CCGG 3 cut(s) 322, 1432, 2040
HhaI GCGC 2 cut(s) 2138, 2304
Hin6I GCGC 2 cut(s) 2136, 2302
HinP1I GCGC 2 cut(s) 2136, 2302
HincII GTYRAC 3 cut(s) 1461, 1498, 1934
HindII GTYRAC 3 cut(s) 1461, 1498, 1934
HindIII AAGCTT 2 cut(s) 1191, 2197
HinfI GANTC 6 cut(s) 152, 635, 983, 1370, 1693, 2238
HpaI GTTAAC 1 cut(s) 1461
HpaII CCGG 3 cut(s) 322, 1432, 2040
HphI GGTGA 7 cut(s) 222, 839, 851, 1067, 1370, 1445, 2276
Hpy166II GTNNAC 7 cut(s) 92, 382, 1199, 1361, 1461, 1498, 1934
Hpy188I TCNGA 6 cut(s) 77, 465, 1278, 1393, 1989, 2285
Hpy188III TCNNGA 8 cut(s) 543, 566, 884, 1425, 1514, 2093, 2141, 2297
Hpy8I GTNNAC 7 cut(s) 92, 382, 1199, 1361, 1461, 1498, 1934
Hpy99I CGWCG 1 cut(s) 1938
HpyAV CCTTC 7 cut(s) 734, 961, 1436, 1528, 1552, 2327, 2395
HpyCH4III ACNGT 9 cut(s) 47, 89, 170, 409, 772, 1159, 1820, 1881, 2101
HpyCH4IV ACGT 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 718, 727, 860, 869, 1190, 1332, 2205
HpyF3I CTNAG 4 cut(s) 699, 760, 1390, 2107
HpySE526I ACGT 1 cut(s) 316
HspAI GCGC 2 cut(s) 2136, 2302
KpnI GGTACC 3 cut(s) 633, 1854, 1968
Ksp22I TGATCA 2 cut(s) 394, 2089
KspAI GTTAAC 1 cut(s) 1461
Kzo9I GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
LmnI GCTCC 4 cut(s) 337, 893, 1246, 2114
LweI GCATC 5 cut(s) 316, 421, 1699, 1780, 2260
MabI ACCWGGT 1 cut(s) 1768
MaeI CTAG 5 cut(s) 1178, 1202, 1572, 2171, 2322
MaeII ACGT 1 cut(s) 316
MalI GATC 9 cut(s) 108, 351, 396, 502, 921, 1512, 1833, 1905, 2091
MboI GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
MboII GAAGA 6 cut(s) 206, 440, 799, 966, 1198, 1555
MfeI CAATTG 2 cut(s) 867, 1521
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 2 cut(s) 1634, 1677
MlsI TGGCCA 1 cut(s) 2349
MluNI TGGCCA 1 cut(s) 2349
MlyI GAGTC 1 cut(s) 1687
MmeI TCCRAC 1 cut(s) 2060
Mox20I TGGCCA 1 cut(s) 2349
Mph1103I ATGCAT 1 cut(s) 1795
MroXI GAANNNNTTC 1 cut(s) 2208
MscI TGGCCA 1 cut(s) 2349
MslI CAYNNNNRTG 4 cut(s) 647, 1160, 1757, 2058
Msp20I TGGCCA 1 cut(s) 2349
MspA1I CMGCKG 2 cut(s) 299, 1415
MspI CCGG 3 cut(s) 322, 1432, 2040
MunI CAATTG 2 cut(s) 867, 1521
Mva1269I GAATGC 3 cut(s) 509, 1983, 2208
MwoI GCNNNNNNNGC 8 cut(s) 11, 718, 727, 860, 869, 1190, 1332, 2205
NciI CCSGG 2 cut(s) 323, 2041
NcoI CCATGG 3 cut(s) 330, 900, 1220
NdeI CATATG 1 cut(s) 1636
NdeII GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
NlaIV GGNNCC 5 cut(s) 375, 631, 1852, 1966, 2160
NmuCI GTSAC 4 cut(s) 170, 745, 772, 2362
NsiI ATGCAT 1 cut(s) 1795
NspI RCATGY 5 cut(s) 26, 523, 880, 1344, 2394
NspV TTCGAA 1 cut(s) 591
PaeR7I CTCGAG 1 cut(s) 2139
PagI TCATGA 1 cut(s) 2296
PceI AGGCCT 1 cut(s) 2344
PciI ACATGT 2 cut(s) 876, 1340
PctI GAATGC 3 cut(s) 509, 1983, 2208
PdmI GAANNNNTTC 1 cut(s) 2208
PfeI GAWTC 5 cut(s) 152, 635, 983, 1370, 2238
PflMI CCANNNNNTGG 1 cut(s) 59
PfoI TCCNGGA 3 cut(s) 276, 321, 1843
PleI GAGTC 1 cut(s) 1687
PpsI GAGTC 1 cut(s) 1687
Ppu21I YACGTR 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 1772
PscI ACATGT 2 cut(s) 876, 1340
PshBI ATTAAT 1 cut(s) 1503
PsiI TTATAA 1 cut(s) 2189
Psp124BI GAGCTC 1 cut(s) 1677
Psp5II RGGWCCY 1 cut(s) 1772
PspEI GGTNACC 1 cut(s) 1655
PspFI CCCAGC 1 cut(s) 675
PspN4I GGNNCC 5 cut(s) 375, 631, 1852, 1966, 2160
PspPI GGNCC 4 cut(s) 861, 1772, 2015, 2159
PspPPI RGGWCCY 1 cut(s) 1772
PstI CTGCAG 2 cut(s) 1290, 1414
PsuI RGATCY 1 cut(s) 500
PvuII CAGCTG 2 cut(s) 299, 1415
RsaI GTAC 8 cut(s) 294, 531, 631, 1138, 1295, 1852, 1966, 2078
RsaNI GTAC 8 cut(s) 293, 530, 630, 1137, 1294, 1851, 1965, 2077
RseI CAYNNNNRTG 4 cut(s) 647, 1160, 1757, 2058
SacI GAGCTC 1 cut(s) 1677
SalI GTCGAC 1 cut(s) 1932
Sau3AI GATC 9 cut(s) 106, 349, 394, 500, 919, 1510, 1831, 1903, 2089
Sau96I GGNCC 4 cut(s) 861, 1772, 2015, 2159
ScaI AGTACT 1 cut(s) 1295
SchI GAGTC 1 cut(s) 1687
SduI GDGCHC 2 cut(s) 1634, 1677
SexAI ACCWGGT 1 cut(s) 1768
SfaNI GCATC 5 cut(s) 316, 421, 1699, 1780, 2260
SfcI CTRYAG 5 cut(s) 378, 437, 915, 1286, 1410
Sfr274I CTCGAG 1 cut(s) 2139
SfuI TTCGAA 1 cut(s) 591
SinI GGWCC 3 cut(s) 1772, 2015, 2159
SlaI CTCGAG 1 cut(s) 2139
SmiMI CAYNNNNRTG 4 cut(s) 647, 1160, 1757, 2058
SmlI CTYRAG 2 cut(s) 991, 2139
SmoI CTYRAG 2 cut(s) 991, 2139
SpeI ACTAGT 1 cut(s) 1177
SseBI AGGCCT 1 cut(s) 2344
SsiI CCGC 3 cut(s) 6, 1960, 1981
SspI AATATT 1 cut(s) 39
SspMI CTAG 5 cut(s) 1178, 1202, 1572, 2171, 2322
SstI GAGCTC 1 cut(s) 1677
StuI AGGCCT 1 cut(s) 2344
StyI CCWWGG 5 cut(s) 189, 330, 900, 1220, 2154
TaaI ACNGT 9 cut(s) 47, 89, 170, 409, 772, 1159, 1820, 1881, 2101
TaiI ACGT 1 cut(s) 319
TaqI TCGA 8 cut(s) 591, 816, 1014, 1509, 1691, 1718, 1933, 2140
TaqII GACCGA 1 cut(s) 2003
TatI WGTACW 2 cut(s) 1136, 1293
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 5 cut(s) 152, 635, 983, 1370, 2238
TscAI CASTG 4 cut(s) 16, 292, 754, 1162
TseFI GTSAC 4 cut(s) 170, 745, 772, 2362
Tsp45I GTSAC 4 cut(s) 170, 745, 772, 2362
TspDTI ATGAA 7 cut(s) 1000, 1067, 1383, 1596, 2042, 2076, 2201
TspGWI ACGGA 3 cut(s) 152, 1877, 2390
TspRI CASTG 4 cut(s) 16, 292, 754, 1162
Van91I CCANNNNNTGG 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 1772, 2015, 2159
VspI ATTAAT 1 cut(s) 1503
XapI RAATTY 3 cut(s) 71, 80, 603
XceI RCATGY 5 cut(s) 26, 523, 880, 1344, 2394
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 2139
XmiI GTMKAC 3 cut(s) 91, 381, 1933
XmnI GAANNNNTTC 1 cut(s) 2208
XspI CTAG 5 cut(s) 1178, 1202, 1572, 2171, 2322
ZrmI AGTACT 1 cut(s) 1295
Zsp2I ATGCAT 1 cut(s) 1795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.