pycom04g05470

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001740
Physical Location & Seq
Reverse (-)
5260597 .. 5262097
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGACGACGGAGTCGGTGAACCTGCCGATCATAGACTTGTCCTCATCGGATTCGATCTCCTCCGCCAATTCAATCCGTCAGGCATGCACGGAGTGTGGTTTCTTCTACCTGGTGAATCATGGCGTCGAGGAAGAGCTGTTTGCAGAGGTGATCGATCAGAGCAGCAACTTCTTTTCGCTTCCTTTGGAAGATAAGTTGAAATTGACTCCCAAGCACCAGAGAGGAGGCTACAGGCCACTATTTTCAGAGAATCTCGACTTCGCTTCCGGCTCCAAAGGTGACTCGAAAGAGAGTTTTAATGTCGGCCCATTAGATGACCCAACTCTGAGTAATTTGAACCAATGGCCTTCAGAAGACATCCTTCCATCTTGGAGGCCTGCAGTGGAATCGTACTTTAGAAAAGTAGTGTCTGCTGGAAAAAGATTAATCTCTCTGATTGCCCTGGCTTTAAACCTGGATGAGGACTTTTTCGACGCGGAGGGGGGGGGGGGGGGGGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

168

Amino Acids

18.26

Weight (kDa)

4.45

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 8 - 118 7.7e-29 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 30
AccII CGCG 1 cut(s) 476
AciI CCGC 2 cut(s) 63, 476
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 93
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 460
AgsI TTSAA 3 cut(s) 72, 199, 337
AjnI CCWGG 3 cut(s) 108, 441, 453
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
AoxI GGCC 4 cut(s) 233, 304, 344, 374
ApeKI GCWGC 1 cut(s) 162
AseI ATTAAT 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 4 cut(s) 28, 124, 160, 290
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 174
BccI CCATC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciT130I CCWGG 3 cut(s) 110, 443, 455
BfmI CTRYAG 2 cut(s) 229, 378
BfuAI ACCTGC 1 cut(s) 30
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
Bme1390I CCNGG 3 cut(s) 110, 443, 455
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 3 cut(s) 110, 443, 455
BpiI GAAGAC 1 cut(s) 360
Bsa29I ATCGAT 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 441
Bsc4I CCNNNNNNNGG 1 cut(s) 460
BseBI CCWGG 3 cut(s) 110, 443, 455
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 2 cut(s) 357, 463
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMII CTCAG 1 cut(s) 317
BseRI GAGGAG 2 cut(s) 49, 237
BseXI GCAGC 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 4 cut(s) 235, 306, 346, 376
BshVI ATCGAT 1 cut(s) 153
BsiSI CCGG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 460
BsnI GGCC 4 cut(s) 235, 306, 346, 376
Bsp143I GATC 4 cut(s) 27, 54, 150, 154
BspACI CCGC 2 cut(s) 63, 476
BspANI GGCC 4 cut(s) 235, 306, 346, 376
BspCNI CTCAG 1 cut(s) 318
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 4 cut(s) 27, 54, 150, 154
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 3 cut(s) 110, 443, 455
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 85, 378
BstDEI CTNAG 1 cut(s) 326
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 2 cut(s) 357, 463
BstFNI CGCG 1 cut(s) 476
BstKTI GATC 4 cut(s) 30, 57, 153, 157
BstMBI GATC 4 cut(s) 27, 54, 150, 154
BstNI CCWGG 3 cut(s) 110, 443, 455
BstNSI RCATGY 1 cut(s) 87
BstSCI CCNGG 3 cut(s) 108, 441, 453
BstSFI CTRYAG 2 cut(s) 229, 378
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 360
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 4 cut(s) 235, 306, 346, 376
BsuTUI ATCGAT 1 cut(s) 153
BtsCI GGATG 2 cut(s) 357, 463
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 85, 378
Cfr13I GGNCC 1 cut(s) 305
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 2 cut(s) 112, 482
CsiI ACCWGGT 1 cut(s) 108
Csp6I GTAC 1 cut(s) 391
CviAII CATG 2 cut(s) 84, 119
CviJI RGCY 8 cut(s) 136, 228, 235, 270, 306, 346, 376, 446
CviKI_1 RGCY 8 cut(s) 136, 228, 235, 270, 306, 346, 376, 446
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 326
DpnI GATC 4 cut(s) 29, 56, 152, 156
DpnII GATC 4 cut(s) 27, 54, 150, 154
DraI TTTAAA 1 cut(s) 450
DraIII CACNNNGTG 1 cut(s) 93
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
EciI GGCGGA 1 cut(s) 52
Eco147I AGGCCT 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoRII CCWGG 3 cut(s) 108, 441, 453
FaeI CATG 2 cut(s) 87, 122
FaiI YATR 3 cut(s) 32, 85, 120
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FatI CATG 2 cut(s) 83, 118
Fnu4HI GCNGC 1 cut(s) 163
FokI GGATG 2 cut(s) 344, 470
Fsp4HI GCNGC 1 cut(s) 163
GluI GCNGC 1 cut(s) 163
HaeIII GGCC 4 cut(s) 235, 306, 346, 376
HapII CCGG 1 cut(s) 267
HgaI GACGC 2 cut(s) 112, 482
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 2 cut(s) 87, 122
HinfI GANTC 7 cut(s) 11, 50, 115, 205, 250, 281, 386
HpaII CCGG 1 cut(s) 267
HphI GGTGA 4 cut(s) 28, 124, 160, 290
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 6 cut(s) 49, 159, 247, 327, 352, 435
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 3 cut(s) 10, 128, 476
HpyAV CCTTC 2 cut(s) 357, 371
HpyCH4V TGCA 3 cut(s) 87, 143, 380
HpyF3I CTNAG 1 cut(s) 326
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 2 cut(s) 87, 122
Kzo9I GATC 4 cut(s) 27, 54, 150, 154
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 1 cut(s) 174
MabI ACCWGGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 29, 56, 152, 156
MboI GATC 4 cut(s) 27, 54, 150, 154
MboII GAAGA 4 cut(s) 94, 143, 200, 365
MluCI AATT 3 cut(s) 67, 200, 331
MlyI GAGTC 3 cut(s) 20, 199, 275
MnlI CCTC 9 cut(s) 52, 70, 121, 139, 215, 218, 366, 454, 472
MseI TTAA 3 cut(s) 297, 425, 449
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 3 cut(s) 110, 443, 455
MvaI CCWGG 3 cut(s) 110, 443, 455
MvnI CGCG 1 cut(s) 476
NdeII GATC 4 cut(s) 27, 54, 150, 154
NlaIII CATG 2 cut(s) 87, 122
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 87
PaeI GCATGC 1 cut(s) 87
PceI AGGCCT 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 126
PfeI GAWTC 4 cut(s) 50, 115, 250, 386
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 164
PleI GAGTC 3 cut(s) 19, 199, 275
PpsI GAGTC 3 cut(s) 19, 199, 275
PshBI ATTAAT 1 cut(s) 425
Psp6I CCWGG 3 cut(s) 108, 441, 453
PspGI CCWGG 3 cut(s) 108, 441, 453
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 305
PstI CTGCAG 1 cut(s) 382
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 297, 425, 449
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 4 cut(s) 27, 54, 150, 154
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 3 cut(s) 20, 199, 275
ScrFI CCNGG 3 cut(s) 110, 443, 455
SetI ASST 6 cut(s) 24, 111, 138, 150, 280, 456
SexAI ACCWGGT 1 cut(s) 108
SfcI CTRYAG 2 cut(s) 229, 378
SphI GCATGC 1 cut(s) 87
Sse9I AATT 3 cut(s) 67, 200, 331
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 2 cut(s) 63, 476
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 3 cut(s) 108, 441, 453
TaqI TCGA 6 cut(s) 53, 126, 153, 255, 284, 471
TasI AATT 3 cut(s) 67, 200, 331
TfiI GAWTC 4 cut(s) 50, 115, 250, 386
Tru1I TTAA 3 cut(s) 297, 425, 449
Tru9I TTAA 3 cut(s) 297, 425, 449
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 1 cut(s) 162
Tsp45I GTSAC 1 cut(s) 278
TspGWI ACGGA 3 cut(s) 23, 65, 104
TspRI CASTG 1 cut(s) 387
Tth111I GACNNNGTC 1 cut(s) 10
VspI ATTAAT 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 458
XceI RCATGY 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.