pycom04g18550

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002437
Physical Location & Seq
Forward (+)
20554702 .. 20556877
2176 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1704 bp
ATGACTCATGATGCATCACTGTGTTTTGGTGGTTGGTACTTAATTAACACATCCGTGAACATCAAGTCTCTTCATCCATGGCTACCAAAACTTCGACCCTCCAAATTTTCACCTAATTTTCCATTTTTCTACCTTCTTCAAACTCAACCAAGTTCTACTACCCCACCGCTTTCAACTTCGGCGATTCAAACTCCGACACAGGCGTGCTTGCTGCCGGCTGATTTCCAGCTTCCACCACCTTATGGACAGTCTTTCTTCAAAACTCCATCTGGGAGGTTTCATGATGCCCGTCTCCTCATAGATTTCTTCTGTAAATTTCTCACAATGCACTTTTGCAATGTTTTTACTTTACCCCACAATTTTACGGTTTATACCACTTTCACATTTGTATGCATTACACATGTAAACGTTTATGGATATTGTTCCAAACATGAGTTACGTCAGTTGGTTAAGGCAGTCACCCACCCCTTACATGTCAATTTGAATCCTCATTCTCATAAATATAATATCACTTTTGTCTTGCCCCTTTGGCTTTCCACATGTGAAAAAGTAAGAGAACTTATGGACACCAAAACCTGCGTCATCCAAATTCTCACATTGATTTCCATCTCCTTACCTCTTTCCAAATCCATCCACTTCAACCACCCTGCCGTTTTCAACTTTGGCGATTCGAATTCCGACACTGGTAATCTTGTTTCCGCCGGCATTGAGAGCATTCTTCCTCCTTATGGACAATCTTATTTCAAAACCCCATCTGGGAGATACTCCGATGGCCGCCTCATCGTTGATTTTCTTTTGGATGCAATGCAGCTTCCATTTCTCAATGCCTATCTGGATTCCGTTGGCCTGCCAATATTCAAAAAAGGTTGCAACTTTGCCGCGGCAGGGTCAACGATACTTCCAGCAACTGCAACATCCATCAGCCCGTTTTCTTTCGGGGTCCAGGTCTCCCAGTTTTTTAGATTCAAAGCTAAGGTTCTTGAATTGCTAGCTAGAGGTAGGAGCTTTCCCAAGTATCTGCCCGCGGAAGTTTTTTTCGCGAAGGGGCTCTACATGTTCGATATAGGCCAGAATGATCTCGCTGGTGCATTTTATTCGAAATCATTGGATCAGATTCTCGCTTCCATTCCAATGATTTTGACAGAATTCGAAACAGGAATAAATAGATTATATGACCAAGGAGGAAGGAACTTTTGGATACACAACACGGGTCCTCTCGGGTGCTTGACGCAGAATGTTGCCAAGTTTGGAACTGATCCTTCGAAGCTTGACGAACAAGGATGCGTCAGCGACCATAACCAAGCTGCTAAACTCTTTAACCTGCAGCTTCATGCCCTCACCAAAAAGTTGCAAGGCCAGTACTCGGATGCAAGTGTCATTTATGTTGATATCTTTTCGATCAAATCTGAGCTCATGTCAAATTATTCGAAAAATGGATTTGAACAGCCTATTATGGCTTGCTGCGGGTATGGAGGTCCACCGTTGAACTATGACAGTCAGATTACCTGCGGACAAACGAAGACCTTGAACGGAACAACTGTCACAGCAAAAGGATGCACTGATAGTTCTGATTACATTAGTTGGGATGGAATTCATTACACCGAAGCTGCGAACCAGTTTGTTTCGTCAAAGATACTCACCGGAAAGTATTCTGATCCGCCCTTCTCGGACAAAATGCCTTTCCTTTTGAAGCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

568

Amino Acids

63.2

Weight (kDa)

8.57

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 218 - 544 7.7e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 584, 1329, 1514
AccB7I CCANNNNNTGG 1 cut(s) 242
AccII CGCG 3 cut(s) 881, 1025, 1040
AclI AACGTT 1 cut(s) 408
AclWI GGATC 3 cut(s) 1116, 1250, 1649
AcoI YGGCCR 1 cut(s) 772
AcsI RAATTY 6 cut(s) 104, 314, 588, 673, 1145, 1590
AfaI GTAC 2 cut(s) 38, 1361
AfiI CCNNNNNNNGG 5 cut(s) 242, 728, 756, 885, 1363
AflIII ACRYGT 4 cut(s) 400, 472, 539, 1053
AjnI CCWGG 1 cut(s) 942
AjuI GAANNNNNNNTTGG 2 cut(s) 1177, 1209
AleI CACNNNNGTG 2 cut(s) 53, 202
Alw21I GWGCWC 1 cut(s) 1413
Alw26I GTCTC 3 cut(s) 72, 296, 952
AlwI GGATC 3 cut(s) 1116, 1250, 1649
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 1217
AoxI GGCC 4 cut(s) 772, 844, 1066, 1354
ApeKI GCWGC 6 cut(s) 211, 808, 1304, 1324, 1461, 1607
ApoI RAATTY 6 cut(s) 104, 314, 588, 673, 1145, 1590
ArsI GACNNNNNNTTYG 2 cut(s) 564, 596
Asp700I GAANNNNTTC 1 cut(s) 1648
AspS9I GGNCC 3 cut(s) 940, 1211, 1475
AsuHPI GGTGA 4 cut(s) 102, 451, 1330, 1630
AsuII TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
AsuNHI GCTAGC 1 cut(s) 988
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 3 cut(s) 940, 1211, 1475
BanII GRGCYC 2 cut(s) 1050, 1413
BarI GAAGNNNNNNTAC 2 cut(s) 1034, 1066
BbsI GAAGAC 1 cut(s) 1526
Bbv12I GWGCWC 1 cut(s) 1413
BbvI GCAGC 6 cut(s) 198, 820, 1291, 1336, 1448, 1594
BccI CCATC 7 cut(s) 274, 614, 638, 760, 764, 926, 1580
BceAI ACGGC 1 cut(s) 635
BcgI CGANNNNNNTGC 2 cut(s) 1077, 1111
BciT130I CCWGG 1 cut(s) 944
BciVI GTATCC 1 cut(s) 1191
BcoDI GTCTC 3 cut(s) 72, 296, 952
BfaI CTAG 2 cut(s) 989, 993
BfmI CTRYAG 1 cut(s) 1322
BfuAI ACCTGC 3 cut(s) 584, 1329, 1514
BfuI GTATCC 1 cut(s) 1191
BglI GCCNNNNNGGC 1 cut(s) 529
BisI GCNGC 9 cut(s) 212, 775, 809, 879, 882, 1305, 1325, 1462, 1608
BlsI GCNGC 9 cut(s) 213, 776, 810, 880, 883, 1306, 1326, 1463, 1609
BmcAI AGTACT 1 cut(s) 1361
Bme1390I CCNGG 1 cut(s) 944
Bme18I GGWCC 3 cut(s) 940, 1211, 1475
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 3 cut(s) 940, 1211, 1475
BmiI GGNNCC 2 cut(s) 941, 1212
BmrFI CCNGG 1 cut(s) 944
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 6 cut(s) 23, 274, 790, 1271, 1357, 1544
BmtI GCTAGC 1 cut(s) 992
BmuI ACTGGG 1 cut(s) 946
BpiI GAAGAC 1 cut(s) 1526
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
BpuEI CTTGAG 1 cut(s) 1679
BsaBI GATNNNNATC 1 cut(s) 605
BsaI GGTCTC 1 cut(s) 952
BsaJI CCNNGG 4 cut(s) 77, 879, 1023, 1177
BsaWI WCCGGW 1 cut(s) 1640
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 728, 756, 885, 1363
Bse118I RCCGGY 2 cut(s) 214, 701
Bse1I ACTGG 4 cut(s) 688, 952, 1357, 1615
Bse3DI GCAATG 2 cut(s) 343, 810
Bse8I GATNNNNATC 1 cut(s) 605
BseBI CCWGG 1 cut(s) 944
BseDI CCNNGG 4 cut(s) 77, 879, 1023, 1177
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 5 cut(s) 242, 728, 756, 885, 1363
BseMI GCAATG 2 cut(s) 343, 810
BseMII CTCAG 1 cut(s) 1398
BseNI ACTGG 4 cut(s) 688, 952, 1357, 1615
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 6 cut(s) 198, 820, 1291, 1336, 1448, 1594
Bsh1236I CGCG 3 cut(s) 881, 1025, 1040
BshFI GGCC 4 cut(s) 774, 846, 1068, 1356
BsiHKAI GWGCWC 1 cut(s) 1413
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 3 cut(s) 215, 702, 1641
BslI CCNNNNNNNGG 5 cut(s) 242, 728, 756, 885, 1363
BsmAI GTCTC 3 cut(s) 72, 296, 952
BsmBI CGTCTC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 4 cut(s) 774, 846, 1068, 1356
Bso31I GGTCTC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 1217
Bsp119I TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
Bsp1286I GDGCHC 2 cut(s) 1050, 1413
Bsp143I GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
Bsp19I CCATGG 1 cut(s) 77
Bsp68I TCGCGA 1 cut(s) 1040
BspANI GGCC 4 cut(s) 774, 846, 1068, 1356
BspCNI CTCAG 1 cut(s) 1399
BspFNI CGCG 3 cut(s) 881, 1025, 1040
BspHI TCATGA 2 cut(s) 7, 280
BspLI GGNNCC 2 cut(s) 941, 1212
BspMAI CTGCAG 1 cut(s) 1326
BspMI ACCTGC 3 cut(s) 584, 1329, 1514
BspOI GCTAGC 1 cut(s) 992
BspPI GGATC 3 cut(s) 1116, 1250, 1649
BspT104I TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
BspTNI GGTCTC 1 cut(s) 952
BsrDI GCAATG 2 cut(s) 343, 810
BsrFI RCCGGY 2 cut(s) 214, 701
BsrI ACTGG 4 cut(s) 688, 952, 1357, 1615
BssAI RCCGGY 2 cut(s) 214, 701
BssECI CCNNGG 4 cut(s) 77, 879, 1023, 1177
BssMI GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
BssT1I CCWWGG 2 cut(s) 77, 1177
Bst2UI CCWGG 1 cut(s) 944
Bst4CI ACNGT 6 cut(s) 21, 249, 367, 1482, 1496, 1540
Bst6I CTCTTC 1 cut(s) 75
BstBI TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
BstC8I GCNNGC 8 cut(s) 205, 209, 216, 703, 848, 990, 1023, 1459
BstDEI CTNAG 2 cut(s) 972, 1407
BstDSI CCRYGG 3 cut(s) 77, 879, 1023
BstFNI CGCG 3 cut(s) 881, 1025, 1040
BstKTI GATC 5 cut(s) 1078, 1111, 1258, 1401, 1657
BstMAI GTCTC 3 cut(s) 72, 296, 952
BstMBI GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
BstMWI GCNNNNNNNGC 2 cut(s) 529, 711
BstNI CCWGG 1 cut(s) 944
BstNSI RCATGY 4 cut(s) 404, 476, 543, 1057
BstSCI CCNGG 1 cut(s) 942
BstSFI CTRYAG 1 cut(s) 1322
BstUI CGCG 3 cut(s) 881, 1025, 1040
BstV1I GCAGC 6 cut(s) 198, 820, 1291, 1336, 1448, 1594
BstV2I GAAGAC 1 cut(s) 1526
BsuI GTATCC 1 cut(s) 1191
BsuRI GGCC 4 cut(s) 774, 846, 1068, 1356
BtgI CCRYGG 3 cut(s) 77, 879, 1023
BtsIMutI CAGTG 3 cut(s) 17, 681, 1557
BtuMI TCGCGA 1 cut(s) 1040
BveI ACCTGC 3 cut(s) 584, 1329, 1514
Cac8I GCNNGC 8 cut(s) 205, 209, 216, 703, 848, 990, 1023, 1459
CaiI CAGNNNCTG 1 cut(s) 908
CciI TCATGA 2 cut(s) 7, 280
Cfr10I RCCGGY 2 cut(s) 214, 701
Cfr13I GGNCC 3 cut(s) 940, 1211, 1475
Cfr42I CCGCGG 2 cut(s) 882, 1026
CseI GACGC 3 cut(s) 568, 1237, 1273
Csp6I GTAC 2 cut(s) 37, 1360
CviQI GTAC 2 cut(s) 37, 1360
DdeI CTNAG 2 cut(s) 972, 1407
DpnI GATC 5 cut(s) 1077, 1110, 1257, 1400, 1656
DpnII GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
EaeI YGGCCR 1 cut(s) 772
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
EciI GGCGGA 2 cut(s) 688, 1647
Ecl136II GAGCTC 1 cut(s) 1411
Eco130I CCWWGG 2 cut(s) 77, 1177
Eco24I GRGCYC 2 cut(s) 1050, 1413
Eco31I GGTCTC 1 cut(s) 952
Eco32I GATATC 1 cut(s) 1390
Eco47I GGWCC 3 cut(s) 940, 1211, 1475
Eco53kI GAGCTC 1 cut(s) 1411
Eco88I CYCGRG 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 1411
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRI GAATTC 3 cut(s) 673, 1145, 1590
EcoRII CCWGG 1 cut(s) 942
EcoRV GATATC 1 cut(s) 1390
EcoT14I CCWWGG 2 cut(s) 77, 1177
EcoT22I ATGCAT 2 cut(s) 16, 395
EcoT38I GRGCYC 2 cut(s) 1050, 1413
ErhI CCWWGG 2 cut(s) 77, 1177
Esp3I CGTCTC 1 cut(s) 296
FauI CCCGC 2 cut(s) 1030, 1457
Fnu4HI GCNGC 9 cut(s) 212, 775, 809, 879, 882, 1305, 1325, 1462, 1608
FriOI GRGCYC 2 cut(s) 1050, 1413
Fsp4HI GCNGC 9 cut(s) 212, 775, 809, 879, 882, 1305, 1325, 1462, 1608
FspBI CTAG 2 cut(s) 989, 993
GluI GCNGC 9 cut(s) 212, 775, 809, 879, 882, 1305, 1325, 1462, 1608
HaeIII GGCC 4 cut(s) 774, 846, 1068, 1356
HapII CCGG 3 cut(s) 215, 702, 1641
HgaI GACGC 3 cut(s) 568, 1237, 1273
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 7 cut(s) 4, 184, 484, 668, 836, 963, 1114
HpaII CCGG 3 cut(s) 215, 702, 1641
HphI GGTGA 4 cut(s) 102, 451, 1330, 1630
Hpy166II GTNNAC 4 cut(s) 58, 406, 891, 1478
Hpy188III TCNNGA 5 cut(s) 8, 281, 833, 980, 1039
Hpy8I GTNNAC 4 cut(s) 58, 406, 891, 1478
HpyAV CCTTC 5 cut(s) 143, 1036, 1179, 1269, 1672
HpyCH4III ACNGT 6 cut(s) 21, 249, 367, 1482, 1496, 1540
HpyCH4IV ACGT 2 cut(s) 408, 439
HpyF10VI GCNNNNNNNGC 2 cut(s) 529, 711
HpyF3I CTNAG 2 cut(s) 972, 1407
HpySE526I ACGT 2 cut(s) 408, 439
KroI GCCGGC 2 cut(s) 214, 701
KroNI GCCGGC 2 cut(s) 216, 703
KspI CCGCGG 2 cut(s) 882, 1026
Kzo9I GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
LmnI GCTCC 1 cut(s) 1002
Lsp1109I GCAGC 6 cut(s) 198, 820, 1291, 1336, 1448, 1594
LweI GCATC 6 cut(s) 23, 274, 790, 1271, 1357, 1544
MaeI CTAG 2 cut(s) 989, 993
MaeII ACGT 2 cut(s) 408, 439
MaeIII GTNAC 3 cut(s) 435, 457, 1540
MalI GATC 5 cut(s) 1077, 1110, 1257, 1400, 1656
MboI GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
MboII GAAGA 6 cut(s) 62, 128, 247, 298, 710, 1531
MhlI GDGCHC 2 cut(s) 1050, 1413
MmeI TCCRAC 2 cut(s) 218, 702
Mph1103I ATGCAT 2 cut(s) 16, 395
MroNI GCCGGC 2 cut(s) 214, 701
MroXI GAANNNNTTC 1 cut(s) 1648
MseI TTAA 4 cut(s) 41, 45, 450, 1317
MslI CAYNNNNRTG 5 cut(s) 19, 53, 202, 388, 1130
MspA1I CMGCKG 2 cut(s) 881, 1025
MspI CCGG 3 cut(s) 215, 702, 1641
MspR9I CCNGG 1 cut(s) 944
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 1 cut(s) 944
MvnI CGCG 3 cut(s) 881, 1025, 1040
MwoI GCNNNNNNNGC 2 cut(s) 529, 711
NaeI GCCGGC 2 cut(s) 216, 703
NcoI CCATGG 1 cut(s) 77
NdeII GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
NgoMIV GCCGGC 2 cut(s) 214, 701
NheI GCTAGC 1 cut(s) 988
NlaIV GGNNCC 2 cut(s) 941, 1212
NmuCI GTSAC 2 cut(s) 457, 1540
NruI TCGCGA 1 cut(s) 1040
NsiI ATGCAT 2 cut(s) 16, 395
NspI RCATGY 4 cut(s) 404, 476, 543, 1057
NspV TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
OliI CACNNNNGTG 2 cut(s) 53, 202
PacI TTAATTAA 1 cut(s) 45
PagI TCATGA 2 cut(s) 7, 280
PciI ACATGT 4 cut(s) 400, 472, 539, 1053
PctI GAATGC 1 cut(s) 714
PdiI GCCGGC 2 cut(s) 216, 703
PdmI GAANNNNTTC 1 cut(s) 1648
PfeI GAWTC 6 cut(s) 184, 484, 668, 836, 963, 1114
PflMI CCANNNNNTGG 1 cut(s) 242
PkrI GCNGC 9 cut(s) 213, 776, 810, 880, 883, 1306, 1326, 1463, 1609
PpuMI RGGWCCY 1 cut(s) 1211
PscI ACATGT 4 cut(s) 400, 472, 539, 1053
Psp124BI GAGCTC 1 cut(s) 1413
Psp1406I AACGTT 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 1 cut(s) 942
PspGI CCWGG 1 cut(s) 942
PspN4I GGNNCC 2 cut(s) 941, 1212
PspPI GGNCC 3 cut(s) 940, 1211, 1475
PspPPI RGGWCCY 1 cut(s) 1211
PstI CTGCAG 1 cut(s) 1326
PstNI CAGNNNCTG 1 cut(s) 908
RruI TCGCGA 1 cut(s) 1040
RsaI GTAC 2 cut(s) 38, 1361
RsaNI GTAC 2 cut(s) 37, 1360
RseI CAYNNNNRTG 5 cut(s) 19, 53, 202, 388, 1130
SacI GAGCTC 1 cut(s) 1413
SacII CCGCGG 2 cut(s) 882, 1026
SaqAI TTAA 4 cut(s) 41, 45, 450, 1317
SatI GCNGC 9 cut(s) 212, 775, 809, 879, 882, 1305, 1325, 1462, 1608
Sau3AI GATC 5 cut(s) 1075, 1108, 1255, 1398, 1654
Sau96I GGNCC 3 cut(s) 940, 1211, 1475
ScaI AGTACT 1 cut(s) 1361
ScrFI CCNGG 1 cut(s) 944
SduI GDGCHC 2 cut(s) 1050, 1413
SfaNI GCATC 6 cut(s) 23, 274, 790, 1271, 1357, 1544
SfcI CTRYAG 1 cut(s) 1322
Sfr303I CCGCGG 2 cut(s) 882, 1026
SfuI TTCGAA 5 cut(s) 671, 1097, 1149, 1262, 1427
SgrBI CCGCGG 2 cut(s) 882, 1026
SinI GGWCC 3 cut(s) 940, 1211, 1475
SmiMI CAYNNNNRTG 5 cut(s) 19, 53, 202, 388, 1130
SmlI CTYRAG 1 cut(s) 1694
SmoI CTYRAG 1 cut(s) 1694
SspI AATATT 1 cut(s) 855
SspMI CTAG 2 cut(s) 989, 993
SstI GAGCTC 1 cut(s) 1413
StyD4I CCNGG 1 cut(s) 942
StyI CCWWGG 2 cut(s) 77, 1177
TaaI ACNGT 6 cut(s) 21, 249, 367, 1482, 1496, 1540
TaiI ACGT 2 cut(s) 411, 442
TaqI TCGA 8 cut(s) 94, 671, 1059, 1097, 1149, 1262, 1397, 1427
TatI WGTACW 1 cut(s) 1359
TauI GCSGC 3 cut(s) 777, 881, 884
TfiI GAWTC 6 cut(s) 184, 484, 668, 836, 963, 1114
Tru1I TTAA 4 cut(s) 41, 45, 450, 1317
Tru9I TTAA 4 cut(s) 41, 45, 450, 1317
TscAI CASTG 3 cut(s) 24, 688, 1564
TseFI GTSAC 2 cut(s) 457, 1540
TseI GCWGC 6 cut(s) 211, 808, 1304, 1324, 1461, 1607
Tsp45I GTSAC 2 cut(s) 457, 1540
TspDTI ATGAA 4 cut(s) 62, 269, 1319, 1583
TspGWI ACGGA 3 cut(s) 43, 829, 1545
TspRI CASTG 3 cut(s) 24, 688, 1564
Van91I CCANNNNNTGG 1 cut(s) 242
VpaK11BI GGWCC 3 cut(s) 940, 1211, 1475
XapI RAATTY 6 cut(s) 104, 314, 588, 673, 1145, 1590
XceI RCATGY 4 cut(s) 404, 476, 543, 1057
XmnI GAANNNNTTC 1 cut(s) 1648
XspI CTAG 2 cut(s) 989, 993
ZrmI AGTACT 1 cut(s) 1361
Zsp2I ATGCAT 2 cut(s) 16, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.