pycom05g06220

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007781
Physical Location & Seq
Reverse (-)
8165173 .. 8167382
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1704 bp
ATGAAGGTCCACCCCAAGCCCAGGAAGCGCAACATCACCGTCCAATACAGATCGAGAAACCCACTTTCTGAAGCGCAGGCACTTTTGGGTCTGACCCATAAGAAGCTCTGCCGTCTCCCCCATGTCTTCAGCCGGATCCTGGAGCTCCCCTTTCGGTCCGACGCTGAAGTTCTGGTCCAGGAGAATCCCGATTGCTTCCGATTCGTCGCCGAAGCCGATAATATCGGCGACGGCGTAAGGACCCACACCATCGAAATCCATCCCGGGGTGACCAAAATCGTGGTTCGCGAAAGTGGATCGGCGGAATTGACGTTGGACGAGCTCGAGCTCGACATGTGGAGGTTTCGGCTGCCGGAATCGACTCGACCCGAGTTGGCCAGCGCGGTTTTCGTGGACGGGGAGCTTATCGTGACGGTGCCGAAGGATGTGGGAGTGGAGAATGCGGACGGAGAGAGAGAATTGCGTCGCCATCGCCACTACCGCCGCCGCATCGTCGGGTTTAGCTTCGGAGAGTCAGCAGAGAGAACTCAAGGAGACCATCATCTCCAATCTTCCATTGGAGAAGAGCTCCAGACCTCCGACCGCAGCACCTACGAGGCCACTACTTCTCCGGAGACGGTGGCGGCGACTCTCCCTCCTTCACTAGCTCCGAGTCCAGCTTCAAAGTCAACTCATCCAACAAAAACCACCACTAGCGACAAATCCACCATCAGACCCAGCAACGGCAGGACAAGAGTCCCAGCCGGACTCTCAGTCCAATCCGCGGCGCCGGCGATGTGCTGCTGCAGTGGGGCCAGAGAAAGAGGTCGAGAGTCTCCAGGACCGAGATCCGAGCTGTCACCGATGAGTCCTCTTCGTCGGCTCAAGCGAGACAAGCTTCGAAGCTGCAGAGGAGAGACAAGTCCTTGGTCCGATCGCCTCCGCCAGCACGGCGTCCACTGCGTCCAAGGGGATCTAACCCAGAAAAAGGATGTGGAAAAAGCTCTGCGGGGAGCAGACTGTGTGTTCCACCTTGCTTCGTATGGCATGTCAGGAAAAGAAACGCTGCAGTTCGGCCATATCTTGGACGCGTGTCTCGAGTTCGGGATTCAAAGGCTGGTTTATGTGAGCACATATAATGTTGTGTTTGGGGGACAGGAGATTGTCAATGGGAACGAGGCCTTGCGCTATTTCCCTATCGAGAACGATGTCGATCCATATGGCCGGAGTAAATCAATTGCCGAGCAGTTGGTCTTAAAGTACAATGCTCGTCCTTTCAGGAAGAAGACTGGGAAGTGTCTTTATACCTGTGCAGTTCGTCCAGCTGCTATCTATGGACCAGGTGAAGAAAGGCACTTTCCAAGGATAATGTCTCTGGCAAAGTTAGGCCTGATTTCGTTCAAAATTGGCGAACCAAATGTGAAAACAGACTGGATTTATGTGGATTTACCTATTGGAGCAAATGAATTAAACAGAGGACAGACTGCACGGAAGGATGAGTTAAGTCTGTCTGATCTCTTAAATCTTGAAATAAAATGGACCTCTAGGCCAGAGAAAATTGAGACTGATCTCTCAAATGAGAACCCCAATCTACCCAAATTACCAGATTTGGCTGGAGTTAATCTCGACAACTTTTTCTTTGAACGAAACAAAGACCGTGCTTTGGGTAGTTCTGAAGGGCTGTTCGGGTCTAACACACAGATTGTCAGTAAAGAGAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

568

Amino Acids

63.44

Weight (kDa)

8.82

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 292 - 373 4.8e-08 NmrA-like family
3Beta_HSD PF01073 300 - 475 1.4e-43 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 308 - 475 1.6e-15 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 308 - 375 7.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 415, 766
AccB7I CCANNNNNTGG 1 cut(s) 1063
AccII CGCG 4 cut(s) 288, 383, 764, 1070
AccIII TCCGGA 1 cut(s) 610
AclWI GGATC 6 cut(s) 130, 143, 304, 822, 960, 1187
AcoI YGGCCR 3 cut(s) 375, 1054, 1201
AcuI CTGAAG 4 cut(s) 90, 112, 186, 1674
AcyI GRCGYC 2 cut(s) 767, 933
AfaI GTAC 1 cut(s) 1241
AfiI CCNNNNNNNGG 8 cut(s) 21, 139, 265, 722, 763, 967, 1063, 1642
AflIII ACRYGT 2 cut(s) 333, 1068
AgsI TTSAA 5 cut(s) 663, 1091, 1381, 1508, 1622
AhdI GACNNNNNGTC 1 cut(s) 752
AjnI CCWGG 5 cut(s) 20, 138, 177, 817, 1318
Alw21I GWGCWC 5 cut(s) 147, 324, 330, 570, 1112
Alw26I GTCTC 9 cut(s) 119, 528, 608, 819, 864, 890, 1079, 1356, 1535
AlwI GGATC 6 cut(s) 130, 143, 304, 822, 960, 1187
Ama87I CYCGRG 4 cut(s) 263, 323, 368, 1076
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 8 cut(s) 375, 597, 792, 1054, 1158, 1201, 1366, 1526
ApeKI GCWGC 7 cut(s) 349, 585, 780, 783, 885, 1045, 1304
AspLEI GCGC 5 cut(s) 30, 76, 383, 769, 1167
AspS9I GGNCC 9 cut(s) 7, 156, 175, 240, 792, 821, 909, 1316, 1518
AsuC2I CCSGG 2 cut(s) 264, 265
AsuHPI GGTGA 4 cut(s) 28, 280, 831, 1334
AsuII TTCGAA 1 cut(s) 880
AvaI CYCGRG 4 cut(s) 263, 323, 368, 1076
AvaII GGWCC 8 cut(s) 7, 156, 175, 240, 821, 909, 1316, 1518
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 135
BanI GGYRCC 2 cut(s) 415, 766
BanII GRGCYC 4 cut(s) 147, 324, 330, 570
BbsI GAAGAC 2 cut(s) 118, 1271
Bbv12I GWGCWC 5 cut(s) 147, 324, 330, 570, 1112
BbvI GCAGC 7 cut(s) 336, 597, 767, 770, 872, 1032, 1291
BccI CCATC 5 cut(s) 257, 267, 477, 546, 716
BceAI ACGGC 4 cut(s) 96, 247, 739, 946
BciT130I CCWGG 5 cut(s) 22, 140, 179, 819, 1320
BcnI CCSGG 2 cut(s) 264, 265
BcoDI GTCTC 9 cut(s) 119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BfaI CTAG 3 cut(s) 644, 693, 1524
BfmI CTRYAG 3 cut(s) 784, 886, 1046
BfoI RGCGCY 1 cut(s) 770
BglI GCCNNNNNGGC 1 cut(s) 930
Bme1390I CCNGG 7 cut(s) 22, 140, 179, 264, 265, 819, 1320
Bme18I GGWCC 8 cut(s) 7, 156, 175, 240, 821, 909, 1316, 1518
BmeRI GACNNNNNGTC 1 cut(s) 752
BmeT110I CYCGRG 4 cut(s) 263, 323, 368, 1076
BmgT120I GGNCC 9 cut(s) 7, 156, 175, 240, 792, 821, 909, 1316, 1518
BmiI GGNNCC 5 cut(s) 137, 242, 417, 768, 793
BmrFI CCNGG 7 cut(s) 22, 140, 179, 264, 265, 819, 1320
BmrI ACTGGG 1 cut(s) 1278
BmsI GCATC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 1278
BoxI GACNNNNGTC 2 cut(s) 734, 1071
BpiI GAAGAC 2 cut(s) 118, 1271
BplI GAGNNNNNCTC 4 cut(s) 552, 584, 1587, 1619
BpmI CTGGAG 4 cut(s) 161, 554, 801, 1614
Bpu14I TTCGAA 1 cut(s) 880
BpuEI CTTGAG 2 cut(s) 513, 848
BpuMI CCSGG 2 cut(s) 264, 265
BsaBI GATNNNNATC 1 cut(s) 1191
BsaHI GRCGYC 2 cut(s) 767, 933
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 7 cut(s) 20, 263, 264, 762, 905, 946, 1340
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 592, 619, 622, 649
Bsc4I CCNNNNNNNGG 8 cut(s) 21, 139, 265, 722, 763, 967, 1063, 1642
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 2 cut(s) 1273, 1415
Bse8I GATNNNNATC 1 cut(s) 1191
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 5 cut(s) 22, 140, 179, 819, 1320
BseDI CCNNGG 7 cut(s) 20, 263, 264, 762, 905, 946, 1340
BseGI GGATG 5 cut(s) 259, 430, 673, 976, 1480
BseJI GATNNNNATC 1 cut(s) 1191
BseLI CCNNNNNNNGG 8 cut(s) 21, 139, 265, 722, 763, 967, 1063, 1642
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 2 cut(s) 1273, 1415
BseRI GAGGAG 1 cut(s) 906
BseXI GCAGC 7 cut(s) 336, 597, 767, 770, 872, 1032, 1291
BseYI CCCAGC 2 cut(s) 716, 739
BsgI GTGCAG 2 cut(s) 1311, 1449
Bsh1236I CGCG 4 cut(s) 288, 383, 764, 1070
Bsh1285I CGRYCG 2 cut(s) 583, 916
BshFI GGCC 8 cut(s) 377, 599, 794, 1056, 1160, 1203, 1368, 1528
BshNI GGYRCC 2 cut(s) 415, 766
BsiEI CGRYCG 2 cut(s) 583, 916
BsiHKAI GWGCWC 5 cut(s) 147, 324, 330, 570, 1112
BsiHKCI CYCGRG 4 cut(s) 263, 323, 368, 1076
BsiSI CCGG 7 cut(s) 133, 264, 353, 611, 744, 770, 1204
BslFI GGGAC 2 cut(s) 722, 1146
BslI CCNNNNNNNGG 8 cut(s) 21, 139, 265, 722, 763, 967, 1063, 1642
BsmAI GTCTC 9 cut(s) 119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BsmBI CGTCTC 2 cut(s) 119, 608
BsmFI GGGAC 2 cut(s) 722, 1146
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 8 cut(s) 377, 599, 794, 1056, 1160, 1203, 1368, 1528
Bso31I GGTCTC 1 cut(s) 528
BsoBI CYCGRG 4 cut(s) 263, 323, 368, 1076
Bsp119I TTCGAA 1 cut(s) 880
Bsp1286I GDGCHC 5 cut(s) 147, 324, 330, 570, 1112
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
Bsp68I TCGCGA 1 cut(s) 288
BspANI GGCC 8 cut(s) 377, 599, 794, 1056, 1160, 1203, 1368, 1528
BspCNI CTCAG 1 cut(s) 764
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 4 cut(s) 288, 383, 764, 1070
BspLI GGNNCC 5 cut(s) 137, 242, 417, 768, 793
BspMAI CTGCAG 3 cut(s) 788, 890, 1050
BspPI GGATC 6 cut(s) 130, 143, 304, 822, 960, 1187
BspQI GCTCTTC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 880
BspT107I GGYRCC 2 cut(s) 415, 766
BspTNI GGTCTC 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 2 cut(s) 1273, 1415
BssAI RCCGGY 1 cut(s) 769
BssECI CCNNGG 7 cut(s) 20, 263, 264, 762, 905, 946, 1340
BssMI GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
BssNI GRCGYC 2 cut(s) 767, 933
BssT1I CCWWGG 3 cut(s) 905, 946, 1340
Bst2UI CCWGG 5 cut(s) 22, 140, 179, 819, 1320
Bst4CI ACNGT 5 cut(s) 40, 415, 619, 1001, 1637
Bst6I CTCTTC 2 cut(s) 558, 858
BstACI GRCGYC 2 cut(s) 767, 933
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 4 cut(s) 78, 379, 771, 926
BstDEI CTNAG 1 cut(s) 751
BstDSI CCRYGG 1 cut(s) 762
BstEII GGTNACC 1 cut(s) 268
BstF5I GGATG 5 cut(s) 259, 430, 673, 976, 1480
BstFNI CGCG 4 cut(s) 288, 383, 764, 1070
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 5 cut(s) 30, 76, 383, 769, 1167
BstKTI GATC 9 cut(s) 53, 138, 299, 830, 916, 955, 1195, 1495, 1549
BstMAI GTCTC 9 cut(s) 119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BstMBI GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
BstMCI CGRYCG 2 cut(s) 583, 916
BstMWI GCNNNNNNNGC 6 cut(s) 25, 480, 770, 874, 930, 939
BstNI CCWGG 5 cut(s) 22, 140, 179, 819, 1320
BstNSI RCATGY 2 cut(s) 337, 1030
BstPAI GACNNNNGTC 2 cut(s) 734, 1071
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 7 cut(s) 20, 138, 177, 262, 263, 817, 1318
BstSFI CTRYAG 3 cut(s) 784, 886, 1046
BstUI CGCG 4 cut(s) 288, 383, 764, 1070
BstV1I GCAGC 7 cut(s) 336, 597, 767, 770, 872, 1032, 1291
BstV2I GAAGAC 2 cut(s) 118, 1271
BstX2I RGATCY 3 cut(s) 135, 827, 952
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 3 cut(s) 135, 827, 952
BsuRI GGCC 8 cut(s) 377, 599, 794, 1056, 1160, 1203, 1368, 1528
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 2 cut(s) 455, 788
BtsCI GGATG 5 cut(s) 259, 430, 673, 976, 1480
BtsI GCAGTG 2 cut(s) 793, 937
BtsIMutI CAGTG 2 cut(s) 793, 937
BtuMI TCGCGA 1 cut(s) 288
Cac8I GCNNGC 4 cut(s) 78, 379, 771, 926
CfoI GCGC 5 cut(s) 30, 76, 383, 769, 1167
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 9 cut(s) 7, 156, 175, 240, 792, 821, 909, 1316, 1518
Cfr42I CCGCGG 1 cut(s) 765
Cfr9I CCCGGG 1 cut(s) 263
CpoI CGGWCCG 1 cut(s) 156
CseI GACGC 5 cut(s) 170, 452, 922, 931, 1076
CsiI ACCWGGT 1 cut(s) 1318
Csp6I GTAC 1 cut(s) 1240
CspI CGGWCCG 1 cut(s) 156
CviAII CATG 3 cut(s) 122, 334, 1027
CviQI GTAC 1 cut(s) 1240
DdeI CTNAG 1 cut(s) 751
DinI GGCGCC 1 cut(s) 768
DpnI GATC 9 cut(s) 52, 137, 298, 829, 915, 954, 1194, 1494, 1548
DpnII GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
DriI GACNNNNNGTC 1 cut(s) 752
EaeI YGGCCR 3 cut(s) 375, 1054, 1201
Eam1104I CTCTTC 2 cut(s) 558, 858
Eam1105I GACNNNNNGTC 1 cut(s) 752
EarI CTCTTC 2 cut(s) 558, 858
EciI GGCGGA 2 cut(s) 317, 911
Ecl136II GAGCTC 4 cut(s) 145, 322, 328, 568
Eco130I CCWWGG 3 cut(s) 905, 946, 1340
Eco147I AGGCCT 2 cut(s) 1160, 1368
Eco24I GRGCYC 4 cut(s) 147, 324, 330, 570
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 8 cut(s) 7, 156, 175, 240, 821, 909, 1316, 1518
Eco53kI GAGCTC 4 cut(s) 145, 322, 328, 568
Eco57I CTGAAG 4 cut(s) 90, 112, 186, 1674
Eco88I CYCGRG 4 cut(s) 263, 323, 368, 1076
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 4 cut(s) 145, 322, 328, 568
EcoO109I RGGNCCY 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 268
EcoRII CCWGG 5 cut(s) 20, 138, 177, 817, 1318
EcoT14I CCWWGG 3 cut(s) 905, 946, 1340
EcoT38I GRGCYC 4 cut(s) 147, 324, 330, 570
EgeI GGCGCC 1 cut(s) 768
EheI GGCGCC 1 cut(s) 768
ErhI CCWWGG 3 cut(s) 905, 946, 1340
Esp3I CGTCTC 2 cut(s) 119, 608
FaeI CATG 3 cut(s) 125, 337, 1030
FaqI GGGAC 2 cut(s) 722, 1146
FatI CATG 3 cut(s) 121, 333, 1026
FauI CCCGC 1 cut(s) 981
FauNDI CATATG 1 cut(s) 1198
FokI GGATG 5 cut(s) 246, 437, 660, 983, 1487
FriOI GRGCYC 4 cut(s) 147, 324, 330, 570
FspBI CTAG 3 cut(s) 644, 693, 1524
GlaI GCGC 5 cut(s) 29, 75, 382, 768, 1166
GsaI CCCAGC 2 cut(s) 720, 743
GsuI CTGGAG 4 cut(s) 161, 554, 801, 1614
HaeII RGCGCY 1 cut(s) 770
HaeIII GGCC 8 cut(s) 377, 599, 794, 1056, 1160, 1203, 1368, 1528
HapII CCGG 7 cut(s) 133, 264, 353, 611, 744, 770, 1204
HgaI GACGC 5 cut(s) 170, 452, 922, 931, 1076
HhaI GCGC 5 cut(s) 30, 76, 383, 769, 1167
Hin1I GRCGYC 2 cut(s) 767, 933
Hin1II CATG 3 cut(s) 125, 337, 1030
Hin6I GCGC 5 cut(s) 28, 74, 381, 767, 1165
HinP1I GCGC 5 cut(s) 28, 74, 381, 767, 1165
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HindIII AAGCTT 1 cut(s) 875
HpaII CCGG 7 cut(s) 133, 264, 353, 611, 744, 770, 1204
HphI GGTGA 4 cut(s) 28, 280, 831, 1334
Hpy166II GTNNAC 4 cut(s) 10, 394, 669, 937
Hpy8I GTNNAC 4 cut(s) 10, 394, 669, 937
Hpy99I CGWCG 6 cut(s) 164, 209, 233, 468, 497, 861
HpyAV CCTTC 4 cut(s) 415, 648, 1465, 1649
HpyCH4III ACNGT 5 cut(s) 40, 415, 619, 1001, 1637
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 5 cut(s) 786, 888, 1048, 1292, 1466
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 480, 770, 874, 930, 939
HpyF3I CTNAG 1 cut(s) 751
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 2 cut(s) 767, 933
Hsp92II CATG 3 cut(s) 125, 337, 1030
HspAI GCGC 5 cut(s) 28, 74, 381, 767, 1165
KasI GGCGCC 1 cut(s) 766
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 1 cut(s) 769
KroNI GCCGGC 1 cut(s) 771
KspI CCGCGG 1 cut(s) 765
Kzo9I GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 7 cut(s) 142, 150, 400, 573, 652, 992, 1436
Lsp1109I GCAGC 7 cut(s) 336, 597, 767, 770, 872, 1032, 1291
LweI GCATC 1 cut(s) 498
MabI ACCWGGT 1 cut(s) 1318
MaeI CTAG 3 cut(s) 644, 693, 1524
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 3 cut(s) 268, 409, 837
MalI GATC 9 cut(s) 52, 137, 298, 829, 915, 954, 1194, 1494, 1548
MboI GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
MboII GAAGA 7 cut(s) 118, 543, 575, 845, 1273, 1276, 1337
MfeI CAATTG 1 cut(s) 1215
MflI RGATCY 3 cut(s) 135, 827, 952
MhlI GDGCHC 5 cut(s) 147, 324, 330, 570, 1112
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 7 cut(s) 305, 458, 1215, 1383, 1445, 1536, 1577
MluI ACGCGT 1 cut(s) 1068
MluNI TGGCCA 1 cut(s) 377
Mly113I GGCGCC 1 cut(s) 767
MlyI GAGTC 8 cut(s) 355, 521, 622, 661, 741, 744, 821, 856
MmeI TCCRAC 4 cut(s) 183, 294, 603, 701
Mox20I TGGCCA 1 cut(s) 377
MreI CGCCGGCG 1 cut(s) 769
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 769
MscI TGGCCA 1 cut(s) 377
MseI TTAA 5 cut(s) 1235, 1448, 1481, 1499, 1599
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 764, 1304
MspI CCGG 7 cut(s) 133, 264, 353, 611, 744, 770, 1204
MspR9I CCNGG 7 cut(s) 22, 140, 179, 264, 265, 819, 1320
MunI CAATTG 1 cut(s) 1215
Mva1269I GAATGC 1 cut(s) 445
MvaI CCWGG 5 cut(s) 22, 140, 179, 819, 1320
MvnI CGCG 4 cut(s) 288, 383, 764, 1070
MwoI GCNNNNNNNGC 6 cut(s) 25, 480, 770, 874, 930, 939
NaeI GCCGGC 1 cut(s) 771
NarI GGCGCC 1 cut(s) 767
NciI CCSGG 2 cut(s) 264, 265
NdeI CATATG 1 cut(s) 1198
NdeII GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
NgoMIV GCCGGC 1 cut(s) 769
NlaIII CATG 3 cut(s) 125, 337, 1030
NlaIV GGNNCC 5 cut(s) 137, 242, 417, 768, 793
NmeAIII GCCGAG 1 cut(s) 1246
NmuCI GTSAC 3 cut(s) 268, 409, 837
NruI TCGCGA 1 cut(s) 288
NspI RCATGY 2 cut(s) 337, 1030
NspV TTCGAA 1 cut(s) 880
PaeR7I CTCGAG 2 cut(s) 323, 1076
PceI AGGCCT 2 cut(s) 1160, 1368
PciI ACATGT 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 558
PcsI WCGNNNNNNNCGW 6 cut(s) 213, 231, 285, 623, 865, 1074
PctI GAATGC 1 cut(s) 445
PdiI GCCGGC 1 cut(s) 771
PfeI GAWTC 4 cut(s) 184, 201, 356, 1087
PflMI CCANNNNNTGG 1 cut(s) 1063
PfoI TCCNGGA 3 cut(s) 138, 177, 817
Ple19I CGATCG 1 cut(s) 916
PleI GAGTC 8 cut(s) 355, 520, 622, 660, 741, 743, 820, 855
PluTI GGCGCC 1 cut(s) 770
PpsI GAGTC 8 cut(s) 355, 520, 622, 660, 741, 743, 820, 855
PpuMI RGGWCCY 1 cut(s) 240
PscI ACATGT 1 cut(s) 333
PshAI GACNNNNGTC 2 cut(s) 734, 1071
Psp124BI GAGCTC 4 cut(s) 147, 324, 330, 570
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 5 cut(s) 20, 138, 177, 817, 1318
PspEI GGTNACC 1 cut(s) 268
PspFI CCCAGC 2 cut(s) 716, 739
PspGI CCWGG 5 cut(s) 20, 138, 177, 817, 1318
PspN4I GGNNCC 5 cut(s) 137, 242, 417, 768, 793
PspPI GGNCC 9 cut(s) 7, 156, 175, 240, 792, 821, 909, 1316, 1518
PspPPI RGGWCCY 1 cut(s) 240
PspXI VCTCGAGB 1 cut(s) 323
PstI CTGCAG 3 cut(s) 788, 890, 1050
PsuI RGATCY 3 cut(s) 135, 827, 952
PvuI CGATCG 1 cut(s) 916
PvuII CAGCTG 1 cut(s) 1304
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 1 cut(s) 1241
RsaNI GTAC 1 cut(s) 1240
Rsr2I CGGWCCG 1 cut(s) 156
RsrII CGGWCCG 1 cut(s) 156
SacI GAGCTC 4 cut(s) 147, 324, 330, 570
SacII CCGCGG 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 5 cut(s) 1235, 1448, 1481, 1499, 1599
Sau3AI GATC 9 cut(s) 50, 135, 296, 827, 913, 952, 1192, 1492, 1546
Sau96I GGNCC 9 cut(s) 7, 156, 175, 240, 792, 821, 909, 1316, 1518
SchI GAGTC 8 cut(s) 355, 521, 622, 661, 741, 744, 821, 856
ScrFI CCNGG 7 cut(s) 22, 140, 179, 264, 265, 819, 1320
SduI GDGCHC 5 cut(s) 147, 324, 330, 570, 1112
SexAI ACCWGGT 1 cut(s) 1318
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 3 cut(s) 784, 886, 1046
SfoI GGCGCC 1 cut(s) 768
Sfr274I CTCGAG 2 cut(s) 323, 1076
Sfr303I CCGCGG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 880
SgrAI CRCCGGYG 1 cut(s) 769
SgrBI CCGCGG 1 cut(s) 765
SinI GGWCC 8 cut(s) 7, 156, 175, 240, 821, 909, 1316, 1518
SlaI CTCGAG 2 cut(s) 323, 1076
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 4 cut(s) 323, 528, 863, 1076
SmoI CTYRAG 4 cut(s) 323, 528, 863, 1076
Sse9I AATT 7 cut(s) 305, 458, 1215, 1383, 1445, 1536, 1577
SseBI AGGCCT 2 cut(s) 1160, 1368
SspDI GGCGCC 1 cut(s) 766
SspMI CTAG 3 cut(s) 644, 693, 1524
SstI GAGCTC 4 cut(s) 147, 324, 330, 570
StuI AGGCCT 2 cut(s) 1160, 1368
StyD4I CCNGG 7 cut(s) 20, 138, 177, 262, 263, 817, 1318
StyI CCWWGG 3 cut(s) 905, 946, 1340
TaaI ACNGT 5 cut(s) 40, 415, 619, 1001, 1637
TaiI ACGT 1 cut(s) 314
TaqII GACCGA 2 cut(s) 144, 838
TasI AATT 7 cut(s) 305, 458, 1215, 1383, 1445, 1536, 1577
TatI WGTACW 1 cut(s) 1239
TauI GCSGC 4 cut(s) 486, 489, 626, 767
TfiI GAWTC 4 cut(s) 184, 201, 356, 1087
Tru1I TTAA 5 cut(s) 1235, 1448, 1481, 1499, 1599
Tru9I TTAA 5 cut(s) 1235, 1448, 1481, 1499, 1599
TscAI CASTG 2 cut(s) 793, 944
TseFI GTSAC 3 cut(s) 268, 409, 837
TseI GCWGC 7 cut(s) 349, 585, 780, 783, 885, 1045, 1304
Tsp45I GTSAC 3 cut(s) 268, 409, 837
TspDTI ATGAA 2 cut(s) 17, 1458
TspGWI ACGGA 2 cut(s) 462, 1483
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 2 cut(s) 793, 944
Van91I CCANNNNNTGG 1 cut(s) 1063
VpaK11BI GGWCC 8 cut(s) 7, 156, 175, 240, 821, 909, 1316, 1518
XceI RCATGY 2 cut(s) 337, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XhoI CTCGAG 2 cut(s) 323, 1076
XmaI CCCGGG 1 cut(s) 263
XspI CTAG 3 cut(s) 644, 693, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.