pycom05g11820

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002066
Physical Location & Seq
Forward (+)
15384646 .. 15388229
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
ATGTTATCAGAGTTGATGAACTTCTTGAGGTCCTGTTTTCGGCCGAGGTCGAATCGATACGTTCATACTGGTGCAGATTCCGGGGGTCGCCAAGACGGGCTCCTGTGGTACAAAGACATAGGCCAGCACATAAATGGTGATTTCTCAATGGCTGTAGTTCAGGCCAACAATTTACTTGAGATCGATCAGAGTCAGCTCGAGTCGGGTAGTTTGAGCACACATGAATCTGGCGCCTATGGTACTTTTGTTGGTGTTTATGACGGCCATGGGGGGCCAGAGACGTCACGCTATATCAATGATCACCTCTTTCAACATCTCAAGAGATTTTGTTTGGAGCAGCAGTCCATGTCTGTGGAGGTAATACGAAAAGCATTTCAAGCAACAGAAGAGGGTTTTCTTTCCGTAGTTACTAGGGAGTGGCCTGTGAAACCACAGATTGCAGCAGTAGGATCGTGCTGCCTTGTTGGTGTTATTTGTAATGGAACTCTTTATATTGCTAACTGTGGTGATTCCCGTGCTGTTTTGGGAAGAATAATGAAGGCAACAGGGGAGGTGCTCTCTATTCAGTTGGTACAAGAGCACAATGCCTCTATAGAATCTGTGAGACAGGAGCTGCAATCCTTGCACCCTGAAGATCCACATATCGTAGTTTTGAAGCATAACGTATGGCGTGTGAAAGGCTTAATACAGATTTCTAGATCTATTGGTGATGTGTATTTAAAAAAGGCTGAGTTTAACAGGGAGCCTTTGTTTTCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATTTTGAGCGCTGATCCAGCAATTTCAGCGATCCAATTGCAGCCACATGATCAATTTATTATATTTGCATCTGATGGGCTCTGGGAACACCTCACCAATCAGGAAGCAGTGGACATAGTTCAAAAACATCCACGAAATGGAAGTGCAAAAAGGCTGATAAAAACCGCCCTGCAAGAAGCAGCAAAAAAGAGAGAGATGAGGTATTCGGACTTGAAGAAGATTGACCGTGGGGTTCGCCGCCATTTCCACGATGACATAACTGTGATCGTCGTGTTTCTTGACTCAAACCTTGTGGGCAAGGCTAGTTCTGTCAAGGGCCCGAACCTATCGGTGAGAGGAGGTGGAATCAACCTGCCTCCAAACACTCTGGCTCCATATGCCACGACAACGGAAGCTGGCAGTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

399

Amino Acids

44.28

Weight (kDa)

8.64

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 80 - 325 1.1e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 1149
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 926
AciI CCGC 2 cut(s) 954, 1027
AclWI GGATC 4 cut(s) 457, 629, 797, 814
AcoI YGGCCR 2 cut(s) 41, 262
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 2 cut(s) 231, 281
AfaI GTAC 4 cut(s) 110, 241, 573, 1192
AfeI AGCGCT 1 cut(s) 799
AfiI CCNNNNNNNGG 2 cut(s) 39, 926
AgsI TTSAA 5 cut(s) 311, 377, 655, 911, 1003
AluBI AGCT 3 cut(s) 196, 613, 1184
AluI AGCT 3 cut(s) 196, 613, 1184
Alw21I GWGCWC 3 cut(s) 218, 558, 582
Alw26I GTCTC 2 cut(s) 272, 598
AlwI GGATC 4 cut(s) 457, 629, 797, 814
AlwNI CAGNNNCTG 2 cut(s) 613, 1194
Ama87I CYCGRG 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 799
AoxI GGCC 7 cut(s) 41, 121, 162, 262, 272, 419, 1105
ApaI GGGCCC 1 cut(s) 1109
ApeKI GCWGC 6 cut(s) 337, 440, 456, 613, 829, 968
AspLEI GCGC 2 cut(s) 233, 800
AspS9I GGNCC 5 cut(s) 30, 272, 772, 1105, 1106
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 6 cut(s) 149, 293, 518, 719, 874, 1132
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 30, 772
BaeGI GKGCMC 1 cut(s) 1109
BaeI ACNNNNGTAYC 2 cut(s) 231, 264
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 3 cut(s) 102, 870, 1109
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 3 cut(s) 218, 558, 582
BbvI GCAGC 6 cut(s) 349, 443, 452, 600, 841, 980
BccI CCATC 1 cut(s) 857
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 432, 466, 808, 842
BclI TGATCA 2 cut(s) 298, 838
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 272, 598
BfaI CTAG 3 cut(s) 411, 696, 1092
BfmI CTRYAG 2 cut(s) 153, 591
BfoI RGCGCY 2 cut(s) 234, 801
BfuAI ACCTGC 1 cut(s) 1149
BglII AGATCT 1 cut(s) 698
BisI GCNGC 7 cut(s) 338, 441, 457, 614, 830, 969, 1027
BlsI GCNGC 7 cut(s) 339, 442, 458, 615, 831, 970, 1028
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 2 cut(s) 30, 772
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 5 cut(s) 30, 272, 772, 1105, 1106
BmiI GGNNCC 8 cut(s) 101, 232, 273, 744, 773, 774, 1107, 1161
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 866
BpiI GAAGAC 1 cut(s) 758
BplI GAGNNNNNCTC 2 cut(s) 542, 574
BpuEI CTTGAG 3 cut(s) 46, 197, 302
BpuMI CCSGG 1 cut(s) 82
Bsa29I ATCGAT 2 cut(s) 55, 183
BsaHI GRCGYC 2 cut(s) 231, 281
BsaJI CCNNGG 4 cut(s) 44, 81, 265, 1015
BsaXI ACNNNNNCTCC 6 cut(s) 326, 356, 734, 764, 1144, 1174
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 926
Bse1I ACTGG 1 cut(s) 73
BseCI ATCGAT 2 cut(s) 55, 183
BseDI CCNNGG 4 cut(s) 44, 81, 265, 1015
BseGI GGATG 1 cut(s) 916
BseLI CCNNNNNNNGG 2 cut(s) 39, 926
BseMII CTCAG 1 cut(s) 720
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 1140
BseSI GKGCMC 1 cut(s) 1109
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 6 cut(s) 349, 443, 452, 600, 841, 980
BsgI GTGCAG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 7 cut(s) 43, 123, 164, 264, 274, 421, 1107
BshNI GGYRCC 1 cut(s) 230
BshVI ATCGAT 2 cut(s) 55, 183
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 3 cut(s) 218, 558, 582
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 2 cut(s) 39, 926
BsmAI GTCTC 2 cut(s) 272, 598
BsmBI CGTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 7 cut(s) 43, 123, 164, 264, 274, 421, 1107
BsoBI CYCGRG 1 cut(s) 197
Bsp120I GGGCCC 1 cut(s) 1105
Bsp1286I GDGCHC 6 cut(s) 102, 218, 558, 582, 870, 1109
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 2 cut(s) 954, 1027
BspANI GGCC 7 cut(s) 43, 123, 164, 264, 274, 421, 1107
BspCNI CTCAG 1 cut(s) 721
BspDI ATCGAT 2 cut(s) 55, 183
BspLI GGNNCC 8 cut(s) 101, 232, 273, 744, 773, 774, 1107, 1161
BspMI ACCTGC 1 cut(s) 1149
BspPI GGATC 4 cut(s) 457, 629, 797, 814
BspT107I GGYRCC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 44, 81, 265, 1015
BssNI GRCGYC 2 cut(s) 231, 281
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 3 cut(s) 503, 1016, 1051
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 2 cut(s) 231, 281
BstAPI GCANNNNNTGC 1 cut(s) 622
BstC8I GCNNGC 2 cut(s) 125, 1186
BstDEI CTNAG 1 cut(s) 729
BstDSI CCRYGG 2 cut(s) 265, 1015
BstF5I GGATG 1 cut(s) 916
BstH2I RGCGCY 2 cut(s) 234, 801
BstHHI GCGC 2 cut(s) 233, 800
BstMAI GTCTC 2 cut(s) 272, 598
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 5 cut(s) 377, 622, 806, 815, 1166
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 2 cut(s) 153, 591
BstSLI GKGCMC 1 cut(s) 1109
BstV1I GCAGC 6 cut(s) 349, 443, 452, 600, 841, 980
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 2 cut(s) 634, 698
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 2 cut(s) 634, 698
BstZI CGGCCG 1 cut(s) 41
Bsu15I ATCGAT 2 cut(s) 55, 183
BsuRI GGCC 7 cut(s) 43, 123, 164, 264, 274, 421, 1107
BsuTUI ATCGAT 2 cut(s) 55, 183
BtgI CCRYGG 2 cut(s) 265, 1015
BtsCI GGATG 1 cut(s) 916
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 1 cut(s) 903
BveI ACCTGC 1 cut(s) 1149
Cac8I GCNNGC 2 cut(s) 125, 1186
CaiI CAGNNNCTG 2 cut(s) 613, 1194
CfoI GCGC 2 cut(s) 233, 800
Cfr13I GGNCC 5 cut(s) 30, 272, 772, 1105, 1106
ClaI ATCGAT 2 cut(s) 55, 183
Csp6I GTAC 4 cut(s) 109, 240, 572, 1191
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 4 cut(s) 221, 266, 346, 836
CviQI GTAC 4 cut(s) 109, 240, 572, 1191
DdeI CTNAG 1 cut(s) 729
DinI GGCGCC 1 cut(s) 232
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 2 cut(s) 41, 262
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 3 cut(s) 102, 870, 1109
Eco47I GGWCC 2 cut(s) 30, 772
Eco47III AGCGCT 1 cut(s) 799
Eco52I CGGCCG 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 197
EcoO109I RGGNCCY 3 cut(s) 30, 772, 1105
EcoT14I CCWWGG 1 cut(s) 265
EcoT38I GRGCYC 3 cut(s) 102, 870, 1109
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 4 cut(s) 224, 269, 349, 839
FaqI GGGAC 1 cut(s) 785
FatI CATG 4 cut(s) 220, 265, 345, 835
FauNDI CATATG 1 cut(s) 1165
FbaI TGATCA 2 cut(s) 298, 838
Fnu4HI GCNGC 7 cut(s) 338, 441, 457, 614, 830, 969, 1027
FokI GGATG 1 cut(s) 903
FriOI GRGCYC 3 cut(s) 102, 870, 1109
Fsp4HI GCNGC 7 cut(s) 338, 441, 457, 614, 830, 969, 1027
FspBI CTAG 3 cut(s) 411, 696, 1092
GlaI GCGC 2 cut(s) 232, 799
GluI GCNGC 7 cut(s) 338, 441, 457, 614, 830, 969, 1027
HaeII RGCGCY 2 cut(s) 234, 801
HaeIII GGCC 7 cut(s) 43, 123, 164, 264, 274, 421, 1107
HapII CCGG 1 cut(s) 81
HhaI GCGC 2 cut(s) 233, 800
Hin1I GRCGYC 2 cut(s) 231, 281
Hin1II CATG 4 cut(s) 224, 269, 349, 839
Hin6I GCGC 2 cut(s) 231, 798
HinP1I GCGC 2 cut(s) 231, 798
HinfI GANTC 9 cut(s) 52, 77, 190, 200, 224, 509, 596, 1070, 1134
HpaII CCGG 1 cut(s) 81
HphI GGTGA 6 cut(s) 149, 293, 518, 719, 874, 1132
Hpy166II GTNNAC 1 cut(s) 901
Hpy188I TCNGA 4 cut(s) 10, 189, 862, 997
Hpy188III TCNNGA 6 cut(s) 25, 319, 696, 770, 890, 1067
Hpy8I GTNNAC 1 cut(s) 901
Hpy99I CGWCG 1 cut(s) 1061
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 503, 1016, 1051
HpyCH4IV ACGT 3 cut(s) 60, 281, 663
HpyCH4V TGCA 8 cut(s) 74, 440, 616, 625, 829, 857, 935, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 377, 622, 806, 815, 1166
HpyF3I CTNAG 1 cut(s) 729
HpySE526I ACGT 3 cut(s) 60, 281, 663
Hsp92I GRCGYC 2 cut(s) 231, 281
Hsp92II CATG 4 cut(s) 224, 269, 349, 839
HspAI GCGC 2 cut(s) 231, 798
KasI GGCGCC 1 cut(s) 230
KflI GGGWCCC 1 cut(s) 772
Ksp22I TGATCA 2 cut(s) 298, 838
LmnI GCTCC 5 cut(s) 105, 334, 610, 742, 1165
Lsp1109I GCAGC 6 cut(s) 349, 443, 452, 600, 841, 980
LweI GCATC 1 cut(s) 866
MaeI CTAG 3 cut(s) 411, 696, 1092
MaeII ACGT 3 cut(s) 60, 281, 663
MaeIII GTNAC 2 cut(s) 282, 406
MboII GAAGA 6 cut(s) 398, 540, 644, 758, 1015, 1018
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 2 cut(s) 634, 698
MhlI GDGCHC 6 cut(s) 102, 218, 558, 582, 870, 1109
MluCI AATT 5 cut(s) 169, 789, 810, 824, 842
Mly113I GGCGCC 1 cut(s) 231
MlyI GAGTC 3 cut(s) 199, 209, 1064
MseI TTAA 3 cut(s) 683, 719, 735
MslI CAYNNNNRTG 4 cut(s) 69, 132, 350, 1049
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 824
MwoI GCNNNNNNNGC 5 cut(s) 377, 622, 806, 815, 1166
NarI GGCGCC 1 cut(s) 231
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 1165
NlaIII CATG 4 cut(s) 224, 269, 349, 839
NlaIV GGNNCC 8 cut(s) 101, 232, 273, 744, 773, 774, 1107, 1161
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 197
PfeI GAWTC 6 cut(s) 52, 77, 224, 509, 596, 1134
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 7 cut(s) 339, 442, 458, 615, 831, 970, 1028
PleI GAGTC 3 cut(s) 198, 208, 1064
PluTI GGCGCC 1 cut(s) 234
PpsI GAGTC 3 cut(s) 198, 208, 1064
PpuMI RGGWCCY 2 cut(s) 30, 772
Psp5II RGGWCCY 2 cut(s) 30, 772
PspN4I GGNNCC 8 cut(s) 101, 232, 273, 744, 773, 774, 1107, 1161
PspOMI GGGCCC 1 cut(s) 1105
PspPI GGNCC 5 cut(s) 30, 272, 772, 1105, 1106
PspPPI RGGWCCY 2 cut(s) 30, 772
PspXI VCTCGAGB 1 cut(s) 197
PstNI CAGNNNCTG 2 cut(s) 613, 1194
PsuI RGATCY 2 cut(s) 634, 698
RsaI GTAC 4 cut(s) 110, 241, 573, 1192
RsaNI GTAC 4 cut(s) 109, 240, 572, 1191
RseI CAYNNNNRTG 4 cut(s) 69, 132, 350, 1049
SaqAI TTAA 3 cut(s) 683, 719, 735
SatI GCNGC 7 cut(s) 338, 441, 457, 614, 830, 969, 1027
Sau96I GGNCC 5 cut(s) 30, 272, 772, 1105, 1106
SchI GAGTC 3 cut(s) 199, 209, 1064
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 6 cut(s) 102, 218, 558, 582, 870, 1109
SfaNI GCATC 1 cut(s) 866
SfcI CTRYAG 2 cut(s) 153, 591
SfoI GGCGCC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 2 cut(s) 30, 772
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 4 cut(s) 69, 132, 350, 1049
SmlI CTYRAG 4 cut(s) 25, 176, 197, 317
SmoI CTYRAG 4 cut(s) 25, 176, 197, 317
Sse9I AATT 5 cut(s) 169, 789, 810, 824, 842
SsiI CCGC 2 cut(s) 954, 1027
SspDI GGCGCC 1 cut(s) 230
SspMI CTAG 3 cut(s) 411, 696, 1092
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 3 cut(s) 503, 1016, 1051
TaiI ACGT 3 cut(s) 63, 284, 666
TaqI TCGA 4 cut(s) 50, 55, 183, 198
TasI AATT 5 cut(s) 169, 789, 810, 824, 842
TauI GCSGC 1 cut(s) 1029
TfiI GAWTC 6 cut(s) 52, 77, 224, 509, 596, 1134
Tru1I TTAA 3 cut(s) 683, 719, 735
Tru9I TTAA 3 cut(s) 683, 719, 735
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 1 cut(s) 282
TseI GCWGC 6 cut(s) 337, 440, 456, 613, 829, 968
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 4 cut(s) 32, 53, 237, 551
TspGWI ACGGA 2 cut(s) 391, 1193
TspRI CASTG 1 cut(s) 903
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 2 cut(s) 30, 772
XbaI TCTAGA 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 197
XspI CTAG 3 cut(s) 411, 696, 1092
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.