pycom05g22640

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005784
Physical Location & Seq
Reverse (-)
24892914 .. 24897859
4946 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3750 bp
ATGATAAACAGAAATTGGGTCTTGAAACGAAAACGTAGAAAGCTTCCTCATGGACCTGATCAATCTAATGGCAAAGAAGAAACTTCAGTGGCCTCAGAATCTCCAGGGAAGACTTCTTCTTCAGATAAGCGCAAGCTGAAAAATGAATTAATTTCTGAGCGGTTTCAATCCAAGAAGAAGGGAAATGATGGGTATTTCTATGAGTGCGTGGTCTGTGACCTTGGCGGTAACTTGTTATGTTGTGATAGCTGTCCCCGAACCTATCATCTCCAGTGTCTTAATCCACCTCTTAAGCGCATTCCGAATGGGAAGTGGCAATGTCCAACTTGCTGTCAGAAAAGAGATCGGAAAAGTAATCTGCTTGAACCCAGAAACTTTTTGACAGAGACCATCTCAAAACGAGCAAGAACAAAACTTGTCACTACTAAGTCCAAAACTGGAATGAAGTCATCTGACAGGGAGAAAGTATCACAGATTTTTGGAAACTCTATTGTTGCAAAGAAAAGATCTTCAAGCAAAGGAAAAGCTGTTTTAACCCATCGAGTCAAATCCTTAGAGAAGAACTCCCAGATAGATATATGTAGCACCAAGCCGACTCATTCAACAGTTGGTGGTTCTGCAGACGGTATTTCATCATGTGTGAATGTTGATGATGAAAAACGATCCAGCATTGTCCCAGAAGAGGATTCCACAGACAGAAAGTCGAGCTCTCCTGCTAAAGAAGTTGCATCTCATTCTAAAGTTACATTGTTAAGGATGAATGATGGAGCTCTTGAGGCATCTGCCTCTCCTGATGTGAAGCCGGATCCATCTTGTACTGATGGATCTCCACATAAGACCATTGTTCTTGCGATCAGTGCTACCACTGACAAGGCTAAAAAAAGAAAACACAAAGGAAATAATCATAAGAGTAAAAAGAAGCCCAGGACTGATAAGGGAAAGTCTGTTGGTATTTCCAAACAATCTGGATCTAAAGCAAATACTACAAAACCGAGGATTGATAAAGCACCTCGTAAGCATAAATCTATCAACCATGGGGTTTCTGCTATTTTGTCAAGAGAAGAAATTGGGACCAAGAAGTCAGATATCCAAAGCAAAGACGAGGAGCTTCCTGAGGGAGCAAAAGACTCATCGCATAATGCAGATAAAGCTGGAAGTCATGTAGTTGAAACAACAATTTGTAGAGATAGTTTTACTGCTCAACCTCTGCAGGTTGATCGGGTTTTGGGATGTCGAGTTCAGGGTGATAATGCTCAATCTTCTCGTCATTTATGTGTGACAGCTGCCCCTGACCTGTGTCCTGCTGATTTGCAAGTTTCTGAGACTCAAAACAGACTATCAGATGGAAATTCTGCTTTTGATAATGACATGGATGTTGGAGCTGCGGACAATGTTGTCAATGGTGCTGATGGGGATGAAAGCATGAAGGTTGAAAATCTCACTGAGGTTTGTGAAAATGTTGTCAGTGGTGCTGATGGGGATGAAAGCACAAAGGATGATGTTAGAGTGGACAAATTACATGTGTACAGAAGATCTGTGAACAAAGAAGGTAAAAAAGCTAATTCCATGGATTTGTCGAGGACGGGTGCTAAGGATTCGAGTCCTGCTAACATAATTTGTAAAGATCAAGATGAATCTGCTGTAACTGCAGATGATTCAGGAAAAAACCCTGAAAAAACAGCGACTATGGAGAATATCGAAGTTAGTTTGAAAAGTCATGATAATGATGAGGTTCCTGAAATTGAAATGCATGCATCTCCTGACACCCAAGATAAAAAAGATGTAGATACAGAAACTGGAATCAGTAGCAGCCCTCAAACCAAAATTGAGGAGCCTTCACTAGCTGAGCCTGCAGGTGGTAGTGATGGGACGGTATTGTATGAATTTTTAGTTAAGTGGGTGGGTAGTTCTCATATTCATAATAGTTGGATTTCTGAATCTGAGCTAAAATCCTTGGCCAAGAGAAAATTAGAAAATTACAAGGCTAAGTATGGAACTGCTGTTATAAATATATGTGAGGAACGGTGGAAGCAACCACAGCGGGTGATTGGTCTCCGTGGTGTTGAAAATGGTTCAGGTGAAGCTTTCATAAAGTGGAATGGTCTCCCTTACGATGAATGTACTTGGGAAAGACTGGATGAGCCTGTCATTAAAAATTCCCAGAATCTGATTGATGAGTTTTATCAGTTTGAACGCCAAACACTGGAAAACAATGCTTCTAAGGATGATTCATCAAAGGGGAAGGTTAATTGTCAGCAAAGTGAAATAGTTACTCTGACAGAGCAGCCTAAGGAACTGAAGGGTATATTGTTTCCTCATCAGCTTGAAGCTCTGAATTGGTTGCGGAAATGCTGGCATAAATCCAAGAATGTAATACTAGCCGATGAGATGGGGCTTGGTAAAACTGTGTCCGCTTGTGCTTTTATTTCATCATTATACGTCGAGTTCAAAGCCACTCTCCCTTGTTTAGTCTTGGTTCCACTTTCCACAATGCGTAATTGGCTTGCTGAGTTTGCATTATGGGCACCTGAATTGAATGTTGTGGAATATCATGGGTGTGCTAAAGCACGAACCATAATACGCCAGCATGAATGGCATGCTAGTGATCCAAATTCATTGATTAAGAAAACATCTGCGTATAAATTTAATGTTCTTTTAACTACATATGAAATGGTTCTTGCTGATTCCTCACATCTCCGCGGGGTTCCTTGGGAAGTTCTCATAGTTGATGAGGGTCATCGTTTGAAAAATTCAGGAAGTAAGCTTTTCAGCTTGCTTAACTCCTTGTCTTTCCAACATCGCGTACTGTTGACTGGTACCCCTCTTCAGAACAACATCGGTGAAATGTATAACTTGCTTAACTTCTTGCAGCCGGCATCATTCCCTTCCCTATCCGCATTTGAGGAAAGGTTTAATGATCTTACAACTGCAGAAAAAGTGGATGAATTGAAAAAACTTGTTGCTCCACATATGCTTCGGAGGCTTAAAAAGGACGCTATGCAAAATATCCCCCCCAAGACTGAACGGATGGTTCCTGTTGAGCTGTCCTCTATCCAAGCTGAATATTACCGTGCAATGCTGACAAAGAATTATCAGATATTGCGGAATATTGGGAAAGGGGTGGCACAGCAGTCGATGCTGAACATCGTAATGCAGTTAAGGAAAGTCTGCAATCATCCGTATCTCATACCAGGCACTGAACCCGACTCTGGGTCTGCTGAGTTCCTTCATGAAATGCGAATTAAAGCTTCAGCCAAGTTGACTCTGTTGCATTCTATGCTTAAGATTTTGCACAAAGAGGGTCACAGAGTCCTCATCTTTTCACAGATGACCAAGCTGCTAGATATCCTTGAGGATTATTTGGCCATAGAATTTGGACCTAAAACATATGAGAGAGTAGACGGCTCTGTTTCAGTGACTGAGCGTCAATCTGCAATTGCGCGTTTTAACCAAGATCGAAGCCGATTTGTCTTTTTGTTATCAACACGCTCTTGCGGCCTTGGTATCAATTTGGCAACAGCAGACACTGTCATTATCTATGATTCTGATTTCAACCCGCACGCTGATATTCAAGCTATGAATCGAGCACATCGAATTGGACAGTCAAAACGACTTTTGGTGTATAGGCTTGTTGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAGAAGAAACTTATGCTTGATCAGCTTTTTGTGAACAAGTCGGTGATCGATCCCAAAAAGAAGTGGAGGATATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1250

Amino Acids

139.33

Weight (kDa)

8.95

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 69 - 110 1.3e-09 PHD-finger
Chromo PF00385 678 - 729 2.1e-09 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 770 - 941 4.1e-10 Type III restriction enzyme, res subunit
DEAD PF00270 772 - 942 7.3e-06 DEAD/DEAH box helicase
SNF2-rel_dom PF00176 773 - 1062 4.9e-79 SNF2-related domain
HDA2-3 PF11496 1010 - 1227 5.9e-13 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 1086 - 1200 8.6e-24 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2006, 3748
AarI CACCTGC 1 cut(s) 1844
AasI GACNNNNNNGTC 2 cut(s) 1078, 1394
Acc36I ACCTGC 2 cut(s) 1201, 1844
Acc65I GGTACC 1 cut(s) 2816
AccB1I GGYRCC 2 cut(s) 2524, 2816
AccB7I CCANNNNNTGG 1 cut(s) 2203
AccBSI CCGCTC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 3399
AccII CGCG 3 cut(s) 2700, 2802, 3442
AclWI GGATC 7 cut(s) 657, 800, 813, 832, 976, 2600, 3716
AcoI YGGCCR 2 cut(s) 1956, 3363
AcsI RAATTY 7 cut(s) 1348, 1883, 2155, 2611, 2642, 2749, 3371
AcuI CTGAAG 5 cut(s) 69, 105, 2318, 2810, 3234
AfaI GTAC 5 cut(s) 817, 1526, 2122, 2805, 2818
AfiI CCNNNNNNNGG 5 cut(s) 682, 1856, 2203, 3209, 3210
AflII CTTAAG 2 cut(s) 290, 3281
AflIII ACRYGT 1 cut(s) 1519
AhdI GACNNNNNGTC 3 cut(s) 700, 3211, 3423
AjnI CCWGG 3 cut(s) 103, 923, 3190
AloI GAACNNNNNNTCC 2 cut(s) 1221, 1253
Alw21I GWGCWC 3 cut(s) 710, 772, 3589
Alw26I GTCTC 4 cut(s) 380, 1316, 2057, 2108
AlwI GGATC 7 cut(s) 657, 800, 813, 832, 976, 2600, 3716
AlwNI CAGNNNCTG 5 cut(s) 1796, 2167, 3197, 3419, 3527
AoxI GGCC 4 cut(s) 90, 1956, 3363, 3496
ApeKI GCWGC 7 cut(s) 1283, 1382, 1809, 2284, 2869, 3337, 3664
ApoI RAATTY 7 cut(s) 1348, 1883, 2155, 2611, 2642, 2749, 3371
AseI ATTAAT 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 2673
Asp718I GGTACC 1 cut(s) 2816
AspLEI GCGC 4 cut(s) 132, 297, 3442, 3657
AspS9I GGNCC 3 cut(s) 53, 1071, 3377
AsuHPI GGTGA 5 cut(s) 1256, 2056, 2090, 2852, 3727
AvaII GGWCC 3 cut(s) 53, 1071, 3377
AxyI CCTNAGG 2 cut(s) 1113, 2289
BaeGI GKGCMC 1 cut(s) 2527
BalI TGGCCA 2 cut(s) 1958, 3365
BamHI GGATCC 1 cut(s) 805
BanI GGYRCC 2 cut(s) 2524, 2816
BanII GRGCYC 2 cut(s) 710, 772
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 3 cut(s) 710, 772, 3589
BbvI GCAGC 7 cut(s) 1270, 1369, 1821, 2296, 2881, 3324, 3676
BceAI ACGGC 1 cut(s) 3418
BcgI CGANNNNNNTGC 4 cut(s) 1199, 1233, 3114, 3148
BciT130I CCWGG 3 cut(s) 105, 925, 3192
BclI TGATCA 2 cut(s) 58, 3691
BcoDI GTCTC 4 cut(s) 380, 1316, 2057, 2108
BfaI CTAG 5 cut(s) 1841, 2378, 2601, 3341, 3642
BfmI CTRYAG 5 cut(s) 618, 1208, 1647, 1851, 2928
BfrI CTTAAG 2 cut(s) 290, 3281
BfuAI ACCTGC 2 cut(s) 1201, 1844
BglII AGATCT 2 cut(s) 506, 1532
BisI GCNGC 8 cut(s) 1284, 1383, 1810, 2285, 2870, 3338, 3496, 3665
BlpI GCTNAGC 1 cut(s) 1845
BlsI GCNGC 8 cut(s) 1285, 1384, 1811, 2286, 2871, 3339, 3497, 3666
Bme1390I CCNGG 3 cut(s) 105, 925, 3192
Bme18I GGWCC 3 cut(s) 53, 1071, 3377
BmeRI GACNNNNNGTC 3 cut(s) 700, 3211, 3423
BmgT120I GGNCC 3 cut(s) 53, 1071, 3377
BmiI GGNNCC 9 cut(s) 807, 1072, 1734, 1833, 2478, 2526, 2706, 2818, 3033
BmrFI CCNGG 3 cut(s) 105, 925, 3192
BmsI GCATC 6 cut(s) 737, 788, 1763, 2885, 3126, 3666
BoxI GACNNNNGTC 1 cut(s) 1296
BpiI GAAGAC 1 cut(s) 116
BplI GAGNNNNNCTC 6 cut(s) 377, 409, 548, 580, 3032, 3064
BpmI CTGGAG 2 cut(s) 87, 254
Bpu10I CCTNAGC 1 cut(s) 1590
Bpu1102I GCTNAGC 1 cut(s) 1845
BpuEI CTTGAG 2 cut(s) 794, 3371
Bsa29I ATCGAT 1 cut(s) 3720
BsaI GGTCTC 3 cut(s) 380, 2057, 2108
Bsc4I CCNNNNNNNGG 5 cut(s) 682, 1856, 2203, 3209, 3210
Bse118I RCCGGY 1 cut(s) 2872
Bse1I ACTGG 6 cut(s) 271, 442, 1801, 2139, 2208, 2818
Bse21I CCTNAGG 2 cut(s) 1113, 2289
Bse3DI GCAATG 2 cut(s) 323, 3081
BseBI CCWGG 3 cut(s) 105, 925, 3192
BseCI ATCGAT 1 cut(s) 3720
BseLI CCNNNNNNNGG 5 cut(s) 682, 1856, 2203, 3209, 3210
BseMI GCAATG 2 cut(s) 323, 3081
BseNI ACTGG 6 cut(s) 271, 442, 1801, 2139, 2208, 2818
BseRI GAGGAG 2 cut(s) 1118, 1844
BseSI GKGCMC 1 cut(s) 2527
BseXI GCAGC 7 cut(s) 1270, 1369, 1821, 2296, 2881, 3324, 3676
Bsh1236I CGCG 3 cut(s) 2700, 2802, 3442
BshFI GGCC 4 cut(s) 92, 1958, 3365, 3498
BshNI GGYRCC 2 cut(s) 2524, 2816
BshVI ATCGAT 1 cut(s) 3720
BsiHKAI GWGCWC 3 cut(s) 710, 772, 3589
BsiSI CCGG 2 cut(s) 803, 2873
BslFI GGGAC 4 cut(s) 237, 659, 1084, 1882
BslI CCNNNNNNNGG 5 cut(s) 682, 1856, 2203, 3209, 3210
BsmAI GTCTC 4 cut(s) 380, 1316, 2057, 2108
BsmFI GGGAC 4 cut(s) 237, 659, 1084, 1882
BsmI GAATGC 2 cut(s) 297, 3271
BsnI GGCC 4 cut(s) 92, 1958, 3365, 3498
Bso31I GGTCTC 3 cut(s) 380, 2057, 2108
Bsp1286I GDGCHC 4 cut(s) 710, 772, 2527, 3589
Bsp1407I TGTACA 1 cut(s) 1524
Bsp1720I GCTNAGC 1 cut(s) 1845
Bsp19I CCATGG 2 cut(s) 1033, 1566
BspANI GGCC 4 cut(s) 92, 1958, 3365, 3498
BspDI ATCGAT 1 cut(s) 3720
BspFNI CGCG 3 cut(s) 2700, 2802, 3442
BspHI TCATGA 2 cut(s) 1717, 3229
BspLI GGNNCC 9 cut(s) 807, 1072, 1734, 1833, 2478, 2526, 2706, 2818, 3033
BspMAI CTGCAG 5 cut(s) 622, 1212, 1651, 1855, 2932
BspMI ACCTGC 2 cut(s) 1201, 1844
BspPI GGATC 7 cut(s) 657, 800, 813, 832, 976, 2600, 3716
BspQI GCTCTTC 1 cut(s) 3645
BspT107I GGYRCC 2 cut(s) 2524, 2816
BspTI CTTAAG 2 cut(s) 290, 3281
BspTNI GGTCTC 3 cut(s) 380, 2057, 2108
BsrBI CCGCTC 1 cut(s) 160
BsrDI GCAATG 2 cut(s) 323, 3081
BsrFI RCCGGY 1 cut(s) 2872
BsrGI TGTACA 1 cut(s) 1524
BsrI ACTGG 6 cut(s) 271, 442, 1801, 2139, 2208, 2818
BssAI RCCGGY 1 cut(s) 2872
BssT1I CCWWGG 6 cut(s) 220, 1033, 1566, 1953, 2708, 3499
Bst2UI CCWGG 3 cut(s) 105, 925, 3192
Bst4CI ACNGT 9 cut(s) 607, 626, 1873, 2025, 2407, 2808, 3071, 3529, 3603
Bst6I CTCTTC 3 cut(s) 675, 2829, 3645
BstAFI CTTAAG 2 cut(s) 290, 3281
BstAPI GCANNNNNTGC 2 cut(s) 3136, 3277
BstAUI TGTACA 1 cut(s) 1524
BstDSI CCRYGG 4 cut(s) 1033, 1566, 2056, 2698
BstFNI CGCG 3 cut(s) 2700, 2802, 3442
BstHHI GCGC 4 cut(s) 132, 297, 3442, 3657
BstMAI GTCTC 4 cut(s) 380, 1316, 2057, 2108
BstNI CCWGG 3 cut(s) 105, 925, 3192
BstNSI RCATGY 3 cut(s) 1523, 1754, 2600
BstPAI GACNNNNGTC 1 cut(s) 1296
BstSCI CCNGG 3 cut(s) 103, 923, 3190
BstSFI CTRYAG 5 cut(s) 618, 1208, 1647, 1851, 2928
BstSLI GKGCMC 1 cut(s) 2527
BstUI CGCG 3 cut(s) 2700, 2802, 3442
BstV1I GCAGC 7 cut(s) 1270, 1369, 1821, 2296, 2881, 3324, 3676
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 5 cut(s) 506, 805, 824, 968, 1532
BstYI RGATCY 5 cut(s) 506, 805, 824, 968, 1532
Bsu15I ATCGAT 1 cut(s) 3720
Bsu36I CCTNAGG 2 cut(s) 1113, 2289
BsuRI GGCC 4 cut(s) 92, 1958, 3365, 3498
BsuTUI ATCGAT 1 cut(s) 3720
BtgI CCRYGG 4 cut(s) 1033, 1566, 2056, 2698
BtgZI GCGATG 2 cut(s) 1116, 2783
BveI ACCTGC 2 cut(s) 1201, 1844
CaiI CAGNNNCTG 5 cut(s) 1796, 2167, 3197, 3419, 3527
CciI TCATGA 2 cut(s) 1717, 3229
CfoI GCGC 4 cut(s) 132, 297, 3442, 3657
Cfr10I RCCGGY 1 cut(s) 2872
Cfr13I GGNCC 3 cut(s) 53, 1071, 3377
Cfr42I CCGCGG 1 cut(s) 2701
ClaI ATCGAT 1 cut(s) 3720
CseI GACGC 2 cut(s) 3002, 3413
Csp6I GTAC 5 cut(s) 816, 1525, 2121, 2804, 2817
CviQI GTAC 5 cut(s) 816, 1525, 2121, 2804, 2817
DrdI GACNNNNNNGTC 2 cut(s) 1078, 1394
DriI GACNNNNNGTC 3 cut(s) 700, 3211, 3423
DseDI GACNNNNNNGTC 2 cut(s) 1078, 1394
EaeI YGGCCR 2 cut(s) 1956, 3363
Eam1104I CTCTTC 3 cut(s) 675, 2829, 3645
Eam1105I GACNNNNNGTC 3 cut(s) 700, 3211, 3423
EarI CTCTTC 3 cut(s) 675, 2829, 3645
Ecl136II GAGCTC 2 cut(s) 708, 770
Eco130I CCWWGG 6 cut(s) 220, 1033, 1566, 1953, 2708, 3499
Eco24I GRGCYC 2 cut(s) 710, 772
Eco31I GGTCTC 3 cut(s) 380, 2057, 2108
Eco32I GATATC 2 cut(s) 1087, 3346
Eco47I GGWCC 3 cut(s) 53, 1071, 3377
Eco53kI GAGCTC 2 cut(s) 708, 770
Eco57I CTGAAG 5 cut(s) 69, 105, 2318, 2810, 3234
Eco81I CCTNAGG 2 cut(s) 1113, 2289
EcoICRI GAGCTC 2 cut(s) 708, 770
EcoRII CCWGG 3 cut(s) 103, 923, 3190
EcoRV GATATC 2 cut(s) 1087, 3346
EcoT14I CCWWGG 6 cut(s) 220, 1033, 1566, 1953, 2708, 3499
EcoT22I ATGCAT 2 cut(s) 1752, 1756
EcoT38I GRGCYC 2 cut(s) 710, 772
ErhI CCWWGG 6 cut(s) 220, 1033, 1566, 1953, 2708, 3499
FaqI GGGAC 4 cut(s) 237, 659, 1084, 1882
FauI CCCGC 3 cut(s) 2034, 2693, 3564
FauNDI CATATG 3 cut(s) 2665, 2970, 3388
FbaI TGATCA 2 cut(s) 58, 3691
FblI GTMKAC 1 cut(s) 3399
Fnu4HI GCNGC 8 cut(s) 1284, 1383, 1810, 2285, 2870, 3338, 3496, 3665
FriOI GRGCYC 2 cut(s) 710, 772
Fsp4HI GCNGC 8 cut(s) 1284, 1383, 1810, 2285, 2870, 3338, 3496, 3665
FspBI CTAG 5 cut(s) 1841, 2378, 2601, 3341, 3642
GlaI GCGC 4 cut(s) 131, 296, 3441, 3656
GluI GCNGC 8 cut(s) 1284, 1383, 1810, 2285, 2870, 3338, 3496, 3665
GsuI CTGGAG 2 cut(s) 87, 254
HaeIII GGCC 4 cut(s) 92, 1958, 3365, 3498
HapII CCGG 2 cut(s) 803, 2873
HgaI GACGC 2 cut(s) 3002, 3413
HhaI GCGC 4 cut(s) 132, 297, 3442, 3657
Hin6I GCGC 4 cut(s) 130, 295, 3440, 3655
HinP1I GCGC 4 cut(s) 130, 295, 3440, 3655
HincII GTYRAC 2 cut(s) 2811, 3261
HindII GTYRAC 2 cut(s) 2811, 3261
HindIII AAGCTT 4 cut(s) 41, 2082, 2762, 3246
HpaII CCGG 2 cut(s) 803, 2873
HphI GGTGA 5 cut(s) 1256, 2056, 2090, 2852, 3727
Hpy166II GTNNAC 7 cut(s) 1510, 1525, 1540, 2811, 3261, 3400, 3706
Hpy8I GTNNAC 7 cut(s) 1510, 1525, 1540, 2811, 3261, 3400, 3706
Hpy99I CGWCG 1 cut(s) 2444
HpyAV CCTTC 8 cut(s) 172, 1420, 1541, 1845, 2236, 2293, 2895, 3236
HpyCH4III ACNGT 9 cut(s) 607, 626, 1873, 2025, 2407, 2808, 3071, 3529, 3603
HpyCH4IV ACGT 2 cut(s) 34, 2439
HpySE526I ACGT 2 cut(s) 34, 2439
HspAI GCGC 4 cut(s) 130, 295, 3440, 3655
KpnI GGTACC 1 cut(s) 2820
KroI GCCGGC 1 cut(s) 2872
KroNI GCCGGC 1 cut(s) 2874
Ksp22I TGATCA 2 cut(s) 58, 3691
KspI CCGCGG 1 cut(s) 2701
LguI GCTCTTC 1 cut(s) 3645
LmnI GCTCC 6 cut(s) 767, 1105, 1118, 1379, 1831, 2968
Lsp1109I GCAGC 7 cut(s) 1270, 1369, 1821, 2296, 2881, 3324, 3676
LweI GCATC 6 cut(s) 737, 788, 1763, 2885, 3126, 3666
MaeI CTAG 5 cut(s) 1841, 2378, 2601, 3341, 3642
MaeII ACGT 2 cut(s) 34, 2439
MaeIII GTNAC 9 cut(s) 215, 227, 418, 742, 1276, 1642, 2269, 3302, 3415
MbiI CCGCTC 1 cut(s) 160
MfeI CAATTG 1 cut(s) 3435
MflI RGATCY 5 cut(s) 506, 805, 824, 968, 1532
MhlI GDGCHC 4 cut(s) 710, 772, 2527, 3589
MlsI TGGCCA 2 cut(s) 1958, 3365
MluNI TGGCCA 2 cut(s) 1958, 3365
MlyI GAGTC 8 cut(s) 552, 589, 1121, 1318, 1609, 3200, 3256, 3318
MmeI TCCRAC 4 cut(s) 347, 1357, 1907, 2818
Mox20I TGGCCA 2 cut(s) 1958, 3365
Mph1103I ATGCAT 2 cut(s) 1752, 1756
MroNI GCCGGC 1 cut(s) 2872
MroXI GAANNNNTTC 1 cut(s) 2673
MscI TGGCCA 2 cut(s) 1958, 3365
MslI CAYNNNNRTG 5 cut(s) 1272, 1722, 2556, 2601, 3149
Msp20I TGGCCA 2 cut(s) 1958, 3365
MspA1I CMGCKG 3 cut(s) 1283, 2041, 2700
MspCI CTTAAG 2 cut(s) 290, 3281
MspI CCGG 2 cut(s) 803, 2873
MspR9I CCNGG 3 cut(s) 105, 925, 3192
MunI CAATTG 1 cut(s) 3435
Mva1269I GAATGC 2 cut(s) 297, 3271
MvaI CCWGG 3 cut(s) 105, 925, 3192
MvnI CGCG 3 cut(s) 2700, 2802, 3442
NaeI GCCGGC 1 cut(s) 2874
NcoI CCATGG 2 cut(s) 1033, 1566
NdeI CATATG 3 cut(s) 2665, 2970, 3388
NgoMIV GCCGGC 1 cut(s) 2872
NlaIV GGNNCC 9 cut(s) 807, 1072, 1734, 1833, 2478, 2526, 2706, 2818, 3033
NmuCI GTSAC 5 cut(s) 215, 418, 1276, 3302, 3415
NsiI ATGCAT 2 cut(s) 1752, 1756
NspI RCATGY 3 cut(s) 1523, 1754, 2600
PaeI GCATGC 2 cut(s) 1754, 2600
PagI TCATGA 2 cut(s) 1717, 3229
PaqCI CACCTGC 1 cut(s) 1844
PciI ACATGT 1 cut(s) 1519
PciSI GCTCTTC 1 cut(s) 3645
PcsI WCGNNNNNNNCGW 1 cut(s) 3589
PctI GAATGC 2 cut(s) 297, 3271
PdiI GCCGGC 1 cut(s) 2874
PdmI GAANNNNTTC 1 cut(s) 2673
PflFI GACNNNGTC 1 cut(s) 3527
PflMI CCANNNNNTGG 1 cut(s) 2203
PkrI GCNGC 8 cut(s) 1285, 1384, 1811, 2286, 2871, 3339, 3497, 3666
PleI GAGTC 8 cut(s) 551, 589, 1121, 1318, 1608, 3200, 3256, 3317
PpsI GAGTC 8 cut(s) 551, 589, 1121, 1318, 1608, 3200, 3256, 3317
PscI ACATGT 1 cut(s) 1519
PshAI GACNNNNGTC 1 cut(s) 1296
PshBI ATTAAT 1 cut(s) 149
PsiI TTATAA 2 cut(s) 2006, 3748
Psp124BI GAGCTC 2 cut(s) 710, 772
Psp6I CCWGG 3 cut(s) 103, 923, 3190
PspGI CCWGG 3 cut(s) 103, 923, 3190
PspN4I GGNNCC 9 cut(s) 807, 1072, 1734, 1833, 2478, 2526, 2706, 2818, 3033
PspPI GGNCC 3 cut(s) 53, 1071, 3377
PstI CTGCAG 5 cut(s) 622, 1212, 1651, 1855, 2932
PstNI CAGNNNCTG 5 cut(s) 1796, 2167, 3197, 3419, 3527
PsuI RGATCY 5 cut(s) 506, 805, 824, 968, 1532
PsyI GACNNNGTC 1 cut(s) 3527
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 5 cut(s) 817, 1526, 2122, 2805, 2818
RsaNI GTAC 5 cut(s) 816, 1525, 2121, 2804, 2817
RseI CAYNNNNRTG 5 cut(s) 1272, 1722, 2556, 2601, 3149
SacI GAGCTC 2 cut(s) 710, 772
SacII CCGCGG 1 cut(s) 2701
SapI GCTCTTC 1 cut(s) 3645
SatI GCNGC 8 cut(s) 1284, 1383, 1810, 2285, 2870, 3338, 3496, 3665
Sau96I GGNCC 3 cut(s) 53, 1071, 3377
SbfI CCTGCAGG 1 cut(s) 1855
SchI GAGTC 8 cut(s) 552, 589, 1121, 1318, 1609, 3200, 3256, 3318
ScrFI CCNGG 3 cut(s) 105, 925, 3192
SdaI CCTGCAGG 1 cut(s) 1855
SduI GDGCHC 4 cut(s) 710, 772, 2527, 3589
SfaNI GCATC 6 cut(s) 737, 788, 1763, 2885, 3126, 3666
SfcI CTRYAG 5 cut(s) 618, 1208, 1647, 1851, 2928
Sfr303I CCGCGG 1 cut(s) 2701
SgrBI CCGCGG 1 cut(s) 2701
SinI GGWCC 3 cut(s) 53, 1071, 3377
SmiMI CAYNNNNRTG 5 cut(s) 1272, 1722, 2556, 2601, 3149
SmlI CTYRAG 4 cut(s) 290, 773, 3281, 3350
SmoI CTYRAG 4 cut(s) 290, 773, 3281, 3350
SphI GCATGC 2 cut(s) 1754, 2600
Sse8387I CCTGCAGG 1 cut(s) 1855
SspI AATATT 2 cut(s) 3065, 3109
SspMI CTAG 5 cut(s) 1841, 2378, 2601, 3341, 3642
SstI GAGCTC 2 cut(s) 710, 772
StyD4I CCNGG 3 cut(s) 103, 923, 3190
StyI CCWWGG 6 cut(s) 220, 1033, 1566, 1953, 2708, 3499
TaaI ACNGT 9 cut(s) 607, 626, 1873, 2025, 2407, 2808, 3071, 3529, 3603
TaiI ACGT 2 cut(s) 37, 2442
TatI WGTACW 3 cut(s) 815, 1524, 2120
TauI GCSGC 1 cut(s) 3498
TseFI GTSAC 5 cut(s) 215, 418, 1276, 3302, 3415
TseI GCWGC 7 cut(s) 1283, 1382, 1809, 2284, 2869, 3337, 3664
Tsp45I GTSAC 5 cut(s) 215, 418, 1276, 3302, 3415
TspGWI ACGGA 3 cut(s) 2045, 3040, 3168
Tth111I GACNNNGTC 1 cut(s) 3527
Van91I CCANNNNNTGG 1 cut(s) 2203
Vha464I CTTAAG 2 cut(s) 290, 3281
VpaK11BI GGWCC 3 cut(s) 53, 1071, 3377
VspI ATTAAT 1 cut(s) 149
XapI RAATTY 7 cut(s) 1348, 1883, 2155, 2611, 2642, 2749, 3371
XceI RCATGY 3 cut(s) 1523, 1754, 2600
XmiI GTMKAC 1 cut(s) 3399
XmnI GAANNNNTTC 1 cut(s) 2673
XspI CTAG 5 cut(s) 1841, 2378, 2601, 3341, 3642
Zsp2I ATGCAT 2 cut(s) 1752, 1756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.