pycom05g24490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002105
Physical Location & Seq
Reverse (-)
26060920 .. 26061174
255 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24490.1

Sequence Viewer

Length: 255 bp
ATGCAACCATGGTGGCAAGGAGAGGAAGCGGCGCCAAGTCACGACCAAAGAGTGAGCAGGTGCGTAAGTGCTGCCATGGCGAAGGAGGTTGGTTATGAGTGGGCAGGGTTTAATTCGACGGAGGAGAGATTGATTGAGAAATTTGGGTTGGATTTGATGCAATGGGATCTGCGGCATGGCCTGCGTAGGACAGAGGAGAGAAACTGGTTGTTTGGTTACAGGAGCAATATTCTTAGAGTTGTTGTTTGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

10.03

Weight (kDa)

6.09

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 31
AciI CCGC 2 cut(s) 29, 172
AclWI GGATC 1 cut(s) 174
AcsI RAATTY 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AlwI GGATC 1 cut(s) 174
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 1 cut(s) 140
AspLEI GCGC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 58
BfoI RGCGCY 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 48
BisI GCNGC 3 cut(s) 30, 72, 173
BlsI GCNGC 3 cut(s) 31, 73, 174
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 8, 75
Bse1I ACTGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 8, 75
BseMI GCAATG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 137, 209
BseXI GCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 31
BsnI GGCC 1 cut(s) 180
Bsp143I GATC 1 cut(s) 166
Bsp19I CCATGG 2 cut(s) 8, 75
BspACI CCGC 2 cut(s) 29, 172
BspANI GGCC 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 48
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 167
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 8, 75
BssMI GATC 1 cut(s) 166
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 2 cut(s) 8, 75
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 1 cut(s) 233
BstDSI CCRYGG 2 cut(s) 8, 75
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 2 cut(s) 77, 181
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 1 cut(s) 180
BtgI CCRYGG 2 cut(s) 8, 75
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 182
CfoI GCGC 1 cut(s) 34
CspCI CAANNNNNGTGG 1 cut(s) 28
CviAII CATG 3 cut(s) 9, 76, 176
CviJI RGCY 1 cut(s) 180
CviKI_1 RGCY 1 cut(s) 180
DdeI CTNAG 1 cut(s) 233
DinI GGCGCC 1 cut(s) 33
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 8, 75
EcoT14I CCWWGG 2 cut(s) 8, 75
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 8, 75
FaeI CATG 3 cut(s) 12, 79, 179
FaiI YATR 5 cut(s) 10, 77, 96, 177, 253
FatI CATG 3 cut(s) 8, 75, 175
Fnu4HI GCNGC 3 cut(s) 30, 72, 173
Fsp4HI GCNGC 3 cut(s) 30, 72, 173
GlaI GCGC 1 cut(s) 33
GluI GCNGC 3 cut(s) 30, 72, 173
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 1 cut(s) 180
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 3 cut(s) 12, 79, 179
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
Hpy188III TCNNGA 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 4, 160
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 181
HpyF3I CTNAG 1 cut(s) 233
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 3 cut(s) 12, 79, 179
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 222
LpnPI CCDG 5 cut(s) 43, 90, 190, 194, 205
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 38, 215
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MflI RGATCY 1 cut(s) 166
MluCI AATT 2 cut(s) 112, 140
Mly113I GGCGCC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 4 cut(s) 16, 79, 115, 187
MseI TTAA 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 77, 181
NarI GGCGCC 1 cut(s) 32
NcoI CCATGG 2 cut(s) 8, 75
NdeII GATC 1 cut(s) 166
NlaIII CATG 3 cut(s) 12, 79, 179
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 38
PaqCI CACCTGC 1 cut(s) 48
PkrI GCNGC 3 cut(s) 31, 73, 174
PluTI GGCGCC 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 33
PsuI RGATCY 1 cut(s) 166
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 30, 72, 173
Sau3AI GATC 1 cut(s) 166
SetI ASST 2 cut(s) 62, 90
SfaNI GCATC 1 cut(s) 147
SfoI GGCGCC 1 cut(s) 33
Sse9I AATT 2 cut(s) 112, 140
SsiI CCGC 2 cut(s) 29, 172
SspDI GGCGCC 1 cut(s) 31
SspI AATATT 1 cut(s) 229
StyI CCWWGG 2 cut(s) 8, 75
TaqI TCGA 1 cut(s) 116
TasI AATT 2 cut(s) 112, 140
TauI GCSGC 2 cut(s) 32, 175
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 38
TspGWI ACGGA 1 cut(s) 134
XapI RAATTY 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.