pycom06g03130

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008811
Physical Location & Seq
Forward (+)
3960961 .. 3964246
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1458 bp
ATGGAGACCATTTTTGCATTTTTGAAAGACAAAGAGACCTTCGATCATGTCAGTCATATACACATCGAAGCTCCTGGTGAAATTGCTGTTACTACAAAGTTGAATTCATCGGATCATAAGACCAGAAGCAACAGTTCTGATGACTTCTCATATCCTTTCAATGTCCGGCATCTTCCACCAATTATATTGACGTGTATATTACCAAAATCATACCCAAGTCACCTACCACCTTATTTTTCAATATCTGTTCAGTGGCTGGATTCTAGTAAAATCTCCAATTTGTGTTCTATGCTGGATTCAATATGGACAGAACAATCAGGACAGGAGGTTATGTACCGATGGGTGGAATGGCTTCAAAATTCTTCTCTCTCTTATCTGGGGTTTGATAAAGAAATAATTCTTGGTCCTTATGGTGTAAGGGCTGCTGTAGATAGACGTGCAGTTACAGGAAGTGTTTCTCCAGATGTTGATATTCCGTTTATTAAAAGTTACAACGATGATAAACTACTTGAGAACTTTTACAATAATTTGCACGAGTGCTGCATCTGTTTCAGTGAGTATGCAGGCACTGAGTTTATCAGGCTACCATGCCGGCATTTCTTCTGTGGGGGATGCATGAAGACTTATTCTGACATACATGTCAAGGAAGGCACCGTACACAAACTGCAGTGTCCAGATGCAAAATGTGGGAGTATGATCCCACCAGGTTTGTTAAAACGATTGCTTGGTGATGAAGAGTTTGAACGATGGGATTCCTTAATGTTGACTAAAACACTAGAGTCAATGTCGGATGTCGTGTACTGCTCAAGATGTGAAACTCCCTGCATAGCGGATGAAGACCAGCATGCCCAATGCGTAAAGTGCTTCTTTAGCTTTTGCACACTTTGCAGGGAGCGGCGGCATGTGGGTGTTGAATGTATGACACCAGAGCTTAAGCTTCGTATCTTGCAGGAACGTCAGAGTTCCTCTCGCTTGAAGGATGATCAAAAACGCAAAGAACTTGAGATGATCAATGAGGTTCTCAGTGTGAGGGAAATTCTTCGCGATGCAAAGCAATGCCCATCGTGTAAGATGGCAATATCCAAAACTGGGGGCTGCAACAAGATGGTGTGTGAAAACTGTGGGCACTACTTCTGCTACCGCTGTGATAAGGAAATTGACGGATATCGATCATTTCAGGTAGATGGTGCATGTGAACTATTCCCGCAAGAAGAAATTCAGAACTGGGAAGCACAGATGAATCCACGACAGGTTTTGGGTCAGATTCAAGCTCAACTCTTTGTTCAGAATGGTCAGGTGTGCCCTAATTGTCGTCAGTTTAATGCCAAGATTGGCAATAACAATCACATGTTCTGCTGGGCGTGTCAAAACCACTATTGCTATTTGTGCAAGAAGATCGTGAGGCGAGGCTCTCATCATTACGGACCTAAAGGTTGCAAACAACACACTGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0023051 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033143 GO:0035257 GO:0035258 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044444 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051427 GO:0051716 GO:0060255 GO:0060765 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1990234 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RWD zf-C3HC4

Protein Analysis

486

Amino Acids

55.79

Weight (kDa)

6.52

Isoelectric Point (pI)

56.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 25 - 121 5.2e-17 RWD domain
IBR PF01485 248 - 306 6.1e-09 IBR domain, a half RING-finger domain
IBR PF01485 348 - 385 7.4e-08 IBR domain, a half RING-finger domain
IBR_1 PF22191 350 - 385 2.1e-09 IBR domain
Rcat_RNF216 PF26200 351 - 392 9.7e-07 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 895
AccII CGCG 1 cut(s) 1044
AciI CCGC 5 cut(s) 830, 895, 898, 1141, 1205
AclWI GGATC 2 cut(s) 120, 691
AcsI RAATTY 4 cut(s) 103, 358, 1035, 1215
AfaI GTAC 3 cut(s) 335, 657, 800
AfiI CCNNNNNNNGG 2 cut(s) 343, 1089
AflII CTTAAG 1 cut(s) 932
AflIII ACRYGT 3 cut(s) 191, 637, 1347
AjiI CACGTC 2 cut(s) 192, 437
AjnI CCWGG 2 cut(s) 73, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 5 cut(s) 71, 873, 931, 937, 1271
AluI AGCT 5 cut(s) 71, 873, 931, 937, 1271
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 2 cut(s) 120, 691
AlwNI CAGNNNCTG 2 cut(s) 256, 569
ApeKI GCWGC 3 cut(s) 422, 540, 1095
ApoI RAATTY 4 cut(s) 103, 358, 1035, 1215
Asp700I GAANNNNTTC 5 cut(s) 351, 396, 454, 1038, 1215
AspS9I GGNCC 2 cut(s) 404, 1424
AsuHPI GGTGA 3 cut(s) 89, 212, 740
AvaII GGWCC 2 cut(s) 404, 1424
BaeGI GKGCMC 2 cut(s) 1128, 1304
BanI GGYRCC 1 cut(s) 650
BarI GAAGNNNNNNTAC 2 cut(s) 639, 671
BauI CACGAG 1 cut(s) 533
BbsI GAAGAC 2 cut(s) 626, 843
BbvI GCAGC 3 cut(s) 409, 527, 1082
BccI CCATC 6 cut(s) 333, 741, 1066, 1069, 1099, 1178
BcgI CGANNNNNNTGC 2 cut(s) 1378, 1412
BciT130I CCWGG 2 cut(s) 75, 705
BclI TGATCA 2 cut(s) 982, 1008
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 264, 776
BfmI CTRYAG 2 cut(s) 426, 665
BfrI CTTAAG 1 cut(s) 932
BisI GCNGC 5 cut(s) 423, 541, 896, 899, 1096
BlsI GCNGC 5 cut(s) 424, 542, 897, 900, 1097
Bme1390I CCNGG 2 cut(s) 75, 705
Bme18I GGWCC 2 cut(s) 404, 1424
BmgBI CACGTC 2 cut(s) 192, 437
BmgT120I GGNCC 2 cut(s) 404, 1424
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 2 cut(s) 75, 705
BmrI ACTGGG 2 cut(s) 1098, 1234
BmsI GCATC 5 cut(s) 178, 552, 602, 667, 1036
BmuI ACTGGG 2 cut(s) 1098, 1234
BpiI GAAGAC 2 cut(s) 626, 843
BplI GAGNNNNNCTC 2 cut(s) 952, 984
BpmI CTGGAG 1 cut(s) 444
BpuEI CTTGAG 3 cut(s) 530, 790, 1022
Bsa29I ATCGAT 1 cut(s) 1168
BsaBI GATNNNNATC 1 cut(s) 1168
BsaI GGTCTC 1 cut(s) 29
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 1089
Bse118I RCCGGY 1 cut(s) 591
Bse1I ACTGG 2 cut(s) 1093, 1229
Bse3DI GCAATG 1 cut(s) 1061
Bse8I GATNNNNATC 1 cut(s) 1168
BseBI CCWGG 2 cut(s) 75, 705
BseCI ATCGAT 1 cut(s) 1168
BseGI GGATG 4 cut(s) 617, 796, 838, 985
BseJI GATNNNNATC 1 cut(s) 1168
BseLI CCNNNNNNNGG 2 cut(s) 343, 1089
BseMI GCAATG 1 cut(s) 1061
BseMII CTCAG 2 cut(s) 561, 1036
BseNI ACTGG 2 cut(s) 1093, 1229
BseSI GKGCMC 2 cut(s) 1128, 1304
BseXI GCAGC 3 cut(s) 409, 527, 1082
BseYI CCCAGC 1 cut(s) 1356
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 1044
BshNI GGYRCC 1 cut(s) 650
BshVI ATCGAT 1 cut(s) 1168
BsiSI CCGG 2 cut(s) 166, 592
BslI CCNNNNNNNGG 2 cut(s) 343, 1089
BsmAI GTCTC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 1128, 1304
Bsp143I GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
Bsp68I TCGCGA 1 cut(s) 1044
BspACI CCGC 5 cut(s) 830, 895, 898, 1141, 1205
BspCNI CTCAG 2 cut(s) 562, 1035
BspDI ATCGAT 1 cut(s) 1168
BspFNI CGCG 1 cut(s) 1044
BspLI GGNNCC 1 cut(s) 652
BspMAI CTGCAG 1 cut(s) 669
BspPI GGATC 2 cut(s) 120, 691
BspT107I GGYRCC 1 cut(s) 650
BspTI CTTAAG 1 cut(s) 932
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 895
BsrDI GCAATG 1 cut(s) 1061
BsrFI RCCGGY 1 cut(s) 591
BsrI ACTGG 2 cut(s) 1093, 1229
BssAI RCCGGY 1 cut(s) 591
BssMI GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
BssSI CACGAG 1 cut(s) 533
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 2 cut(s) 75, 705
Bst4CI ACNGT 3 cut(s) 134, 655, 1121
Bst6I CTCTTC 1 cut(s) 729
BstAFI CTTAAG 1 cut(s) 932
BstAPI GCANNNNNTGC 1 cut(s) 885
BstC8I GCNNGC 3 cut(s) 565, 593, 846
BstDEI CTNAG 2 cut(s) 570, 1022
BstF5I GGATG 4 cut(s) 617, 796, 838, 985
BstFNI CGCG 1 cut(s) 1044
BstKTI GATC 7 cut(s) 46, 115, 699, 985, 1011, 1172, 1398
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
BstMWI GCNNNNNNNGC 4 cut(s) 861, 870, 885, 1386
BstNI CCWGG 2 cut(s) 75, 705
BstNSI RCATGY 5 cut(s) 641, 848, 905, 1194, 1351
BstSCI CCNGG 2 cut(s) 73, 703
BstSFI CTRYAG 2 cut(s) 426, 665
BstSLI GKGCMC 2 cut(s) 1128, 1304
BstUI CGCG 1 cut(s) 1044
BstV1I GCAGC 3 cut(s) 409, 527, 1082
BstV2I GAAGAC 2 cut(s) 626, 843
Bsu15I ATCGAT 1 cut(s) 1168
BsuTUI ATCGAT 1 cut(s) 1168
BtgZI GCGATG 1 cut(s) 1059
BtrI CACGTC 2 cut(s) 192, 437
BtsCI GGATG 4 cut(s) 617, 796, 838, 985
BtsI GCAGTG 1 cut(s) 674
BtsIMutI CAGTG 6 cut(s) 257, 559, 567, 674, 1030, 1446
BtuMI TCGCGA 1 cut(s) 1044
Cac8I GCNNGC 3 cut(s) 565, 593, 846
CaiI CAGNNNCTG 2 cut(s) 256, 569
Cfr10I RCCGGY 1 cut(s) 591
Cfr13I GGNCC 2 cut(s) 404, 1424
ClaI ATCGAT 1 cut(s) 1168
CsiI ACCWGGT 1 cut(s) 703
Csp6I GTAC 3 cut(s) 334, 656, 799
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviAII CATG 8 cut(s) 47, 588, 616, 638, 845, 902, 1191, 1348
CviQI GTAC 3 cut(s) 334, 656, 799
DdeI CTNAG 2 cut(s) 570, 1022
DpnI GATC 7 cut(s) 45, 114, 698, 984, 1010, 1171, 1397
DpnII GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
Eam1104I CTCTTC 1 cut(s) 729
EarI CTCTTC 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 29
Eco32I GATATC 1 cut(s) 1166
Eco47I GGWCC 2 cut(s) 404, 1424
EcoRI GAATTC 1 cut(s) 103
EcoRII CCWGG 2 cut(s) 73, 703
EcoRV GATATC 1 cut(s) 1166
EcoT22I ATGCAT 1 cut(s) 617
FaeI CATG 8 cut(s) 50, 591, 619, 641, 848, 905, 1194, 1351
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FatI CATG 8 cut(s) 46, 587, 615, 637, 844, 901, 1190, 1347
FauI CCCGC 1 cut(s) 1212
FbaI TGATCA 2 cut(s) 982, 1008
Fnu4HI GCNGC 5 cut(s) 423, 541, 896, 899, 1096
FokI GGATG 4 cut(s) 624, 803, 845, 992
Fsp4HI GCNGC 5 cut(s) 423, 541, 896, 899, 1096
FspBI CTAG 2 cut(s) 264, 776
GluI GCNGC 5 cut(s) 423, 541, 896, 899, 1096
GsaI CCCAGC 1 cut(s) 1360
GsuI CTGGAG 1 cut(s) 444
HapII CCGG 2 cut(s) 166, 592
Hin1II CATG 8 cut(s) 50, 591, 619, 641, 848, 905, 1194, 1351
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 6 cut(s) 260, 296, 752, 779, 1240, 1264
HpaII CCGG 2 cut(s) 166, 592
HphI GGTGA 3 cut(s) 89, 212, 740
Hpy166II GTNNAC 4 cut(s) 658, 765, 799, 1196
Hpy188I TCNGA 8 cut(s) 112, 139, 631, 790, 960, 1221, 1263, 1287
Hpy188III TCNNGA 6 cut(s) 318, 461, 674, 807, 1043, 1399
Hpy8I GTNNAC 4 cut(s) 658, 765, 799, 1196
HpyAV CCTTC 4 cut(s) 49, 641, 970, 1445
HpyCH4III ACNGT 3 cut(s) 134, 655, 1121
HpyCH4IV ACGT 3 cut(s) 191, 436, 955
HpyF10VI GCNNNNNNNGC 4 cut(s) 861, 870, 885, 1386
HpyF3I CTNAG 2 cut(s) 570, 1022
HpySE526I ACGT 3 cut(s) 191, 436, 955
Hsp92II CATG 8 cut(s) 50, 591, 619, 641, 848, 905, 1194, 1351
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
Ksp22I TGATCA 2 cut(s) 982, 1008
Kzo9I GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
LmnI GCTCC 2 cut(s) 76, 892
Lsp1109I GCAGC 3 cut(s) 409, 527, 1082
LweI GCATC 5 cut(s) 178, 552, 602, 667, 1036
MabI ACCWGGT 1 cut(s) 703
MaeI CTAG 2 cut(s) 264, 776
MaeII ACGT 3 cut(s) 191, 436, 955
MaeIII GTNAC 4 cut(s) 88, 218, 442, 488
MalI GATC 7 cut(s) 45, 114, 698, 984, 1010, 1171, 1397
MbiI CCGCTC 1 cut(s) 895
MboI GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
MboII GAAGA 9 cut(s) 164, 354, 592, 631, 746, 848, 1031, 1223, 1405
MhlI GDGCHC 2 cut(s) 1128, 1304
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 6 cut(s) 319, 976, 1009, 1023, 1395, 1400
Mph1103I ATGCAT 1 cut(s) 617
MroNI GCCGGC 1 cut(s) 591
MroXI GAANNNNTTC 5 cut(s) 351, 396, 454, 1038, 1215
MseI TTAA 5 cut(s) 483, 713, 758, 933, 1320
MslI CAYNNNNRTG 1 cut(s) 906
MspA1I CMGCKG 1 cut(s) 1143
MspCI CTTAAG 1 cut(s) 932
MspI CCGG 2 cut(s) 166, 592
MspR9I CCNGG 2 cut(s) 75, 705
MvaI CCWGG 2 cut(s) 75, 705
MvnI CGCG 1 cut(s) 1044
MwoI GCNNNNNNNGC 4 cut(s) 861, 870, 885, 1386
NaeI GCCGGC 1 cut(s) 593
NdeII GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
NgoMIV GCCGGC 1 cut(s) 591
NlaIII CATG 8 cut(s) 50, 591, 619, 641, 848, 905, 1194, 1351
NlaIV GGNNCC 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 218
NruI TCGCGA 1 cut(s) 1044
NsiI ATGCAT 1 cut(s) 617
NspI RCATGY 5 cut(s) 641, 848, 905, 1194, 1351
PaeI GCATGC 1 cut(s) 848
PciI ACATGT 2 cut(s) 637, 1347
PdiI GCCGGC 1 cut(s) 593
PdmI GAANNNNTTC 5 cut(s) 351, 396, 454, 1038, 1215
PfeI GAWTC 5 cut(s) 260, 296, 752, 1240, 1264
PkrI GCNGC 5 cut(s) 424, 542, 897, 900, 1097
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
PscI ACATGT 2 cut(s) 637, 1347
Psp6I CCWGG 2 cut(s) 73, 703
PspFI CCCAGC 1 cut(s) 1356
PspGI CCWGG 2 cut(s) 73, 703
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 2 cut(s) 404, 1424
PstI CTGCAG 1 cut(s) 669
PstNI CAGNNNCTG 2 cut(s) 256, 569
RruI TCGCGA 1 cut(s) 1044
RsaI GTAC 3 cut(s) 335, 657, 800
RsaNI GTAC 3 cut(s) 334, 656, 799
RseI CAYNNNNRTG 1 cut(s) 906
SaqAI TTAA 5 cut(s) 483, 713, 758, 933, 1320
SatI GCNGC 5 cut(s) 423, 541, 896, 899, 1096
Sau3AI GATC 7 cut(s) 43, 112, 696, 982, 1008, 1169, 1395
Sau96I GGNCC 2 cut(s) 404, 1424
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 2 cut(s) 75, 705
SduI GDGCHC 2 cut(s) 1128, 1304
SexAI ACCWGGT 1 cut(s) 703
SfaNI GCATC 5 cut(s) 178, 552, 602, 667, 1036
SfcI CTRYAG 2 cut(s) 426, 665
SinI GGWCC 2 cut(s) 404, 1424
SmiMI CAYNNNNRTG 1 cut(s) 906
SmlI CTYRAG 4 cut(s) 509, 805, 932, 1001
SmoI CTYRAG 4 cut(s) 509, 805, 932, 1001
SphI GCATGC 1 cut(s) 848
SsiI CCGC 5 cut(s) 830, 895, 898, 1141, 1205
SspMI CTAG 2 cut(s) 264, 776
StyD4I CCNGG 2 cut(s) 73, 703
TaaI ACNGT 3 cut(s) 134, 655, 1121
TaiI ACGT 3 cut(s) 194, 439, 958
TaqI TCGA 3 cut(s) 42, 66, 1168
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 898, 901
TfiI GAWTC 5 cut(s) 260, 296, 752, 1240, 1264
Tru1I TTAA 5 cut(s) 483, 713, 758, 933, 1320
Tru9I TTAA 5 cut(s) 483, 713, 758, 933, 1320
TscAI CASTG 6 cut(s) 257, 559, 574, 674, 1030, 1453
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 3 cut(s) 422, 540, 1095
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 5 cut(s) 96, 632, 747, 849, 1253
TspGWI ACGGA 3 cut(s) 465, 1176, 1437
TspRI CASTG 6 cut(s) 257, 559, 574, 674, 1030, 1453
Vha464I CTTAAG 1 cut(s) 932
VpaK11BI GGWCC 2 cut(s) 404, 1424
XapI RAATTY 4 cut(s) 103, 358, 1035, 1215
XceI RCATGY 5 cut(s) 641, 848, 905, 1194, 1351
XmnI GAANNNNTTC 5 cut(s) 351, 396, 454, 1038, 1215
XspI CTAG 2 cut(s) 264, 776
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.