pycom06g10570
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003307
Physical Location & Seq
Forward (+)
15871144 .. 15872418
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGGGACCTGTGAAGAAGGCTATGGACGACGCTGGCTTGGAGAAGCGCCAGATTGATGAGATTGTGCTTGTTGGTGGAAGTACCAGGATTCCCAAGGTCCAACAGCTTCTTAAGGACTACTTTGATGGCAAGGAGCCAAACAAGGGTGTGAACCCTGATGAGGCTGTTGCTTATGGTGCTGCTGTACAAGGAAGCATCTTAAGTGGAGAGGGTGGTGAAGAAACCAAAGATATTCTTCTCCTTGACGTCGCACCTCTCACCCTCGGTATTGAAACTGTTGGAGGAGTGATGACCAAGCTTATCCCAAGAAACACCGTGATCCCAACCAAGAAATCCCAGGTTTTCACCACCTACCAGGATCAGCAGACAACCGTCTCCATTCAGGTCTTTGAGGGTGAAAGGAGTCTCACAAAGGATTGCAGGAACCTCGGTAAATTTGATCTTTCCGGAATTCCTCCAGCTCCAAGGGGAACTCCTCAAATCGAAGTCACATTCGAAGTGGATGCCAACGGTATCCTGAACGTCAGGGCAGAAGACAAGGGAACTGGTAAATCCGAGAAGATCACCATCACAAACGACAAGGGTCGTCTCAGCCAGGAGGAGATTGAGCGCATGGTTAAGGAGGCAGAAGAGTTTGCCGAGGAAGACAAGAAGGTGAAGGAGAGAATCGATGCCCGCAACAGCCTCGAGACCTATGTCTACAACATGAAGAACCAGGTCAATGACAAAGACAAGCTTGCTGACAAGCTTGAGTCCGATGAGAAGGAAAAGATTGAGACCGCGACAAAGGAAGCCCTCGAGTGGTTGGACGACAACCAGACCGCCGAGAAGGAAGACTATGACGAGAAGCTCAAGGAGGTCGAAGCCGTGTGCAACCCCATCATCTCAGCCGTCTACCAGAGGTCAGGAGGTGCCCCGGGTGGTGCCGGAGCCTCGGAGGAGGACGATGAGTCACATGACGAGCTTTAA

Protein Analysis

323

Amino Acids

35.42

Weight (kDa)

4.6

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 2 - 300 1.2e-110 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 911, 923
AccI GTMKAC 2 cut(s) 699, 894
AccII CGCG 1 cut(s) 782
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 3 cut(s) 676, 780, 822
AclWI GGATC 2 cut(s) 313, 366
AcsI RAATTY 2 cut(s) 434, 450
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 2 cut(s) 82, 186
AfiI CCNNNNNNNGG 3 cut(s) 143, 160, 801
AflII CTTAAG 2 cut(s) 110, 199
AgsI TTSAA 1 cut(s) 272
AhdI GACNNNNNGTC 1 cut(s) 949
AjnI CCWGG 5 cut(s) 83, 336, 354, 594, 714
AluBI AGCT 7 cut(s) 106, 298, 461, 736, 748, 850, 964
AluI AGCT 7 cut(s) 106, 298, 461, 736, 748, 850, 964
Alw26I GTCTC 5 cut(s) 379, 410, 593, 683, 770
AlwI GGATC 2 cut(s) 313, 366
Ama87I CYCGRG 3 cut(s) 686, 797, 916
Aor13HI TCCGGA 1 cut(s) 446
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 434, 450
AspLEI GCGC 2 cut(s) 48, 612
AspS9I GGNCC 2 cut(s) 5, 97
AsuC2I CCSGG 2 cut(s) 917, 918
AsuHPI GGTGA 6 cut(s) 227, 250, 337, 407, 556, 667
AsuII TTCGAA 1 cut(s) 495
AvaI CYCGRG 3 cut(s) 686, 797, 916
AvaII GGWCC 2 cut(s) 5, 97
BaeGI GKGCMC 1 cut(s) 916
BanI GGYRCC 2 cut(s) 911, 923
BbsI GAAGAC 3 cut(s) 540, 651, 840
BbvI GCAGC 1 cut(s) 166
BccI CCATC 3 cut(s) 119, 575, 887
BceAI ACGGC 2 cut(s) 851, 875
BcgI CGANNNNNNTGC 6 cut(s) 409, 443, 485, 519, 667, 701
BciT130I CCWGG 5 cut(s) 85, 338, 356, 596, 716
BciVI GTATCC 1 cut(s) 524
BcnI CCSGG 2 cut(s) 917, 918
BcoDI GTCTC 5 cut(s) 379, 410, 593, 683, 770
BfoI RGCGCY 1 cut(s) 49
BfrI CTTAAG 2 cut(s) 110, 199
BfuI GTATCC 1 cut(s) 524
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 7 cut(s) 85, 338, 356, 596, 716, 917, 918
Bme18I GGWCC 2 cut(s) 5, 97
BmeRI GACNNNNNGTC 1 cut(s) 949
BmeT110I CYCGRG 3 cut(s) 686, 797, 916
BmgT120I GGNCC 2 cut(s) 5, 97
BmiI GGNNCC 6 cut(s) 6, 135, 425, 913, 925, 931
BmrFI CCNGG 7 cut(s) 85, 338, 356, 596, 716, 917, 918
BmsI GCATC 3 cut(s) 204, 493, 661
BoxI GACNNNNGTC 3 cut(s) 371, 582, 695
BpiI GAAGAC 3 cut(s) 540, 651, 840
BpmI CTGGAG 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 770, 836
BpuMI CCSGG 2 cut(s) 917, 918
Bsa29I ATCGAT 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 683, 770
BsaJI CCNNGG 9 cut(s) 93, 262, 336, 427, 464, 639, 915, 916, 933
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 160, 801
Bse1I ACTGG 1 cut(s) 550
Bse8I GATNNNNATC 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 446
BseBI CCWGG 5 cut(s) 85, 338, 356, 596, 716
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 9 cut(s) 93, 262, 336, 427, 464, 639, 915, 916, 933
BseGI GGATG 1 cut(s) 508
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 3 cut(s) 143, 160, 801
BseMII CTCAG 2 cut(s) 604, 900
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 4 cut(s) 297, 465, 614, 953
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 782
BshNI GGYRCC 2 cut(s) 911, 923
BshVI ATCGAT 1 cut(s) 669
BsiHKCI CYCGRG 3 cut(s) 686, 797, 916
BsiSI CCGG 3 cut(s) 447, 917, 927
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 143, 160, 801
BsmAI GTCTC 5 cut(s) 379, 410, 593, 683, 770
BsmBI CGTCTC 2 cut(s) 379, 593
BsmFI GGGAC 1 cut(s) 18
Bso31I GGTCTC 2 cut(s) 683, 770
BsoBI CYCGRG 3 cut(s) 686, 797, 916
Bsp119I TTCGAA 1 cut(s) 495
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 446
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 318, 358, 439, 561
BspACI CCGC 3 cut(s) 676, 780, 822
BspCNI CTCAG 2 cut(s) 603, 899
BspDI ATCGAT 1 cut(s) 669
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 782
BspLI GGNNCC 6 cut(s) 6, 135, 425, 913, 925, 931
BspPI GGATC 2 cut(s) 313, 366
BspT104I TTCGAA 1 cut(s) 495
BspT107I GGYRCC 2 cut(s) 911, 923
BspTI CTTAAG 2 cut(s) 110, 199
BspTNI GGTCTC 2 cut(s) 683, 770
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 9 cut(s) 93, 262, 336, 427, 464, 639, 915, 916, 933
BssMI GATC 4 cut(s) 318, 358, 439, 561
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 93, 464
Bst2UI CCWGG 5 cut(s) 85, 338, 356, 596, 716
Bst4CI ACNGT 4 cut(s) 277, 316, 373, 512
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 2 cut(s) 110, 199
BstAUI TGTACA 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 495
BstC8I GCNNGC 3 cut(s) 34, 676, 738
BstDEI CTNAG 2 cut(s) 590, 886
BstF5I GGATG 1 cut(s) 508
BstFNI CGCG 1 cut(s) 782
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 2 cut(s) 48, 612
BstKTI GATC 4 cut(s) 321, 361, 442, 564
BstMAI GTCTC 5 cut(s) 379, 410, 593, 683, 770
BstMBI GATC 4 cut(s) 318, 358, 439, 561
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 5 cut(s) 85, 338, 356, 596, 716
BstPAI GACNNNNGTC 3 cut(s) 371, 582, 695
BstSCI CCNGG 7 cut(s) 83, 336, 354, 594, 714, 915, 916
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 1 cut(s) 782
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 3 cut(s) 540, 651, 840
Bsu15I ATCGAT 1 cut(s) 669
BsuI GTATCC 1 cut(s) 524
BsuTUI ATCGAT 1 cut(s) 669
BtsCI GGATG 1 cut(s) 508
Cac8I GCNNGC 3 cut(s) 34, 676, 738
CfoI GCGC 2 cut(s) 48, 612
Cfr13I GGNCC 2 cut(s) 5, 97
Cfr9I CCCGGG 1 cut(s) 916
ClaI ATCGAT 1 cut(s) 669
CseI GACGC 1 cut(s) 38
CsiI ACCWGGT 1 cut(s) 714
Csp6I GTAC 2 cut(s) 81, 185
CviAII CATG 3 cut(s) 613, 706, 956
CviQI GTAC 2 cut(s) 81, 185
DdeI CTNAG 2 cut(s) 590, 886
DpnI GATC 4 cut(s) 320, 360, 441, 563
DpnII GATC 4 cut(s) 318, 358, 439, 561
DriI GACNNNNNGTC 1 cut(s) 949
Eam1104I CTCTTC 1 cut(s) 624
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 93, 464
Eco31I GGTCTC 2 cut(s) 683, 770
Eco47I GGWCC 2 cut(s) 5, 97
Eco88I CYCGRG 3 cut(s) 686, 797, 916
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 450
EcoRII CCWGG 5 cut(s) 83, 336, 354, 594, 714
EcoT14I CCWWGG 2 cut(s) 93, 464
ErhI CCWWGG 2 cut(s) 93, 464
Esp3I CGTCTC 2 cut(s) 379, 593
FaeI CATG 3 cut(s) 616, 709, 959
FaiI YATR 7 cut(s) 23, 174, 614, 696, 707, 840, 957
FalI AAGNNNNNCTT 6 cut(s) 104, 136, 219, 251, 720, 752
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 612, 705, 955
FauI CCCGC 1 cut(s) 683
FblI GTMKAC 2 cut(s) 699, 894
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 1 cut(s) 180
GlaI GCGC 2 cut(s) 47, 611
GluI GCNGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 49
HapII CCGG 3 cut(s) 447, 917, 927
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 48, 612
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 3 cut(s) 616, 709, 959
Hin6I GCGC 2 cut(s) 46, 610
HinP1I GCGC 2 cut(s) 46, 610
HindIII AAGCTT 3 cut(s) 296, 734, 746
HinfI GANTC 5 cut(s) 88, 403, 666, 752, 950
HpaII CCGG 3 cut(s) 447, 917, 927
HphI GGTGA 6 cut(s) 227, 250, 337, 407, 556, 667
Hpy166II GTNNAC 3 cut(s) 151, 700, 895
Hpy188I TCNGA 3 cut(s) 556, 757, 937
Hpy188III TCNNGA 4 cut(s) 447, 517, 688, 906
Hpy8I GTNNAC 3 cut(s) 151, 700, 895
Hpy99I CGWCG 2 cut(s) 32, 251
HpyAV CCTTC 5 cut(s) 10, 646, 652, 757, 823
HpyCH4III ACNGT 4 cut(s) 277, 316, 373, 512
HpyCH4IV ACGT 2 cut(s) 246, 522
HpyCH4V TGCA 2 cut(s) 420, 873
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 2 cut(s) 590, 886
HpySE526I ACGT 2 cut(s) 246, 522
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 3 cut(s) 616, 709, 959
HspAI GCGC 2 cut(s) 46, 610
Kpn2I TCCGGA 1 cut(s) 446
Kzo9I GATC 4 cut(s) 318, 358, 439, 561
LmnI GCTCC 3 cut(s) 133, 466, 929
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 3 cut(s) 204, 493, 661
MabI ACCWGGT 1 cut(s) 714
MaeII ACGT 2 cut(s) 246, 522
MaeIII GTNAC 2 cut(s) 487, 951
MalI GATC 4 cut(s) 320, 360, 441, 563
MboI GATC 4 cut(s) 318, 358, 439, 561
MboII GAAGA 9 cut(s) 25, 227, 230, 545, 571, 641, 656, 721, 845
MhlI GDGCHC 1 cut(s) 916
MluCI AATT 2 cut(s) 434, 450
MlyI GAGTC 3 cut(s) 412, 761, 959
MmeI TCCRAC 3 cut(s) 124, 259, 786
MroI TCCGGA 1 cut(s) 446
MseI TTAA 4 cut(s) 111, 200, 618, 967
MspCI CTTAAG 2 cut(s) 110, 199
MspI CCGG 3 cut(s) 447, 917, 927
MspR9I CCNGG 7 cut(s) 85, 338, 356, 596, 716, 917, 918
MvaI CCWGG 5 cut(s) 85, 338, 356, 596, 716
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 1 cut(s) 176
NciI CCSGG 2 cut(s) 917, 918
NdeII GATC 4 cut(s) 318, 358, 439, 561
NlaIII CATG 3 cut(s) 616, 709, 959
NlaIV GGNNCC 6 cut(s) 6, 135, 425, 913, 925, 931
NmeAIII GCCGAG 2 cut(s) 664, 850
NmuCI GTSAC 2 cut(s) 487, 951
NspV TTCGAA 1 cut(s) 495
PaeR7I CTCGAG 2 cut(s) 686, 797
PfeI GAWTC 2 cut(s) 88, 666
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 3 cut(s) 411, 760, 958
PpsI GAGTC 3 cut(s) 411, 760, 958
PpuMI RGGWCCY 1 cut(s) 5
PshAI GACNNNNGTC 3 cut(s) 371, 582, 695
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 5 cut(s) 83, 336, 354, 594, 714
PspGI CCWGG 5 cut(s) 83, 336, 354, 594, 714
PspN4I GGNNCC 6 cut(s) 6, 135, 425, 913, 925, 931
PspPI GGNCC 2 cut(s) 5, 97
PspPPI RGGWCCY 1 cut(s) 5
PspXI VCTCGAGB 1 cut(s) 797
RsaI GTAC 2 cut(s) 82, 186
RsaNI GTAC 2 cut(s) 81, 185
SaqAI TTAA 4 cut(s) 111, 200, 618, 967
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 4 cut(s) 318, 358, 439, 561
Sau96I GGNCC 2 cut(s) 5, 97
SchI GAGTC 3 cut(s) 412, 761, 959
ScrFI CCNGG 7 cut(s) 85, 338, 356, 596, 716, 917, 918
SduI GDGCHC 1 cut(s) 916
SexAI ACCWGGT 1 cut(s) 714
SfaNI GCATC 3 cut(s) 204, 493, 661
Sfr274I CTCGAG 2 cut(s) 686, 797
SfuI TTCGAA 1 cut(s) 495
SinI GGWCC 2 cut(s) 5, 97
SlaI CTCGAG 2 cut(s) 686, 797
SmaI CCCGGG 1 cut(s) 918
SmlI CTYRAG 6 cut(s) 110, 199, 686, 749, 797, 851
SmoI CTYRAG 6 cut(s) 110, 199, 686, 749, 797, 851
Sse9I AATT 2 cut(s) 434, 450
SsiI CCGC 3 cut(s) 676, 780, 822
StyD4I CCNGG 7 cut(s) 83, 336, 354, 594, 714, 915, 916
StyI CCWWGG 2 cut(s) 93, 464
TaaI ACNGT 4 cut(s) 277, 316, 373, 512
TaiI ACGT 2 cut(s) 249, 525
TaqI TCGA 6 cut(s) 483, 495, 669, 687, 798, 861
TasI AATT 2 cut(s) 434, 450
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 2 cut(s) 88, 666
Tru1I TTAA 4 cut(s) 111, 200, 618, 967
Tru9I TTAA 4 cut(s) 111, 200, 618, 967
TseFI GTSAC 2 cut(s) 487, 951
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 2 cut(s) 487, 951
TspDTI ATGAA 1 cut(s) 722
TspMI CCCGGG 1 cut(s) 916
Vha464I CTTAAG 2 cut(s) 110, 199
VpaK11BI GGWCC 2 cut(s) 5, 97
XapI RAATTY 2 cut(s) 434, 450
XhoI CTCGAG 2 cut(s) 686, 797
XmaI CCCGGG 1 cut(s) 916
XmiI GTMKAC 2 cut(s) 699, 894
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.