pycom06g17560
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004694
Physical Location & Seq
Forward (+)
21494915 .. 21498455
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3294 bp
ATGTACTTATCAACTTCGAAAACTGGTTCATGGCGTGCGGTGTGTTCAATATTCTTGGTCTTAGACGTGATCATGATCACAGGTGCTTCCGTTGTAGTCGGAGATTCTCTGGACACAGACAGAGAAGTTCTGCTGAGCCTCAAAGCATTTCTGCAGCAAAAGAACCGCGTAAACCAAGGAATATACTCGCAGTGGAACCAGCGCAGCAGCAACCCCTGCGAGTGGTCCGGAATTTCATGCAGCAATGACAGCAAGAGAGTTAGCCGTGTTGAGCTCAGCGACCAAAAAATCACCGGTGAGATTTTCCGAAACTTCTCTGCCCTTACAGCACTTTCGTACCTCGACCTCTCGAAGAACACAATAAGCGGAGCACTCCCGGAGGACTTGAGTCAATGCCACAGCCTCAGACACCTCAATCTGTCACATAACATTATTGATGGCCAGCTGAACTTGAATGGTTTGAATAAGTTGGAGGTTCTTGATTTGACTGTCAATAGATTTTACGGCAATTTGAAGATGACCTTTCCCGGAGTTTGCAGTAACCTAGTGGTTGCGAATCTTTCAGCGAATAATTTCACCGGAAGGATTGATAGCCTGTTTGATGAATGCTTTAATTTGCAGCACTTGGATTTGAGCTCAAACTATCTTAGTGGTGAGATATGGAGTGGATTCATCAGGCTTACGGCGTTTTCAGTATCCGAAAACTATTTCAGTGGCGCTATTTCGCCTTCTGTTTTCGCAAATAATTGTAGCCTGGTAAAGTTGGACTTGTCAGTAAACAGGTTTTCTGGTGCAGTTCCTGCAGAGATTTCGAAATGCCAGAGACTGATCATATTGAATCTTTGGGGGAACCATTTTACGGGGCCAATCCCGCCTGAGATTGGATCAATTTCGGGACTGCAGGCCTTGTTCTTGGGTAACAACAGTTTTTCTAGAGATATTCCTGAATCCCTTTTGGATTTGACCAGCATGACATTCTTGGATTTGAGCAGGAACGACTTTGGCGGCAAGATACAAGACATTTTTGGGAGATTCAGACAGGTGAAGTTTCTTTTGCTGCACTCAAACGGGTACACCGGTGGTATCCATTCGTCTGGGATTCTCAAGCTACCAAATGTTTCCAGGTTAGACCTCAGCTACAACAATTTTACAGGTCCTCTGCCAGTCGAAATTTCTCTGCCGAAGTTGAAGTTATTTGTCCTCGCCTTTAATCAATTCAACGGAACTATACCACCAGACTATGGAAACATTTCGAGCCTCCAAGCCCTGGACCTTTCCTTCAACAGCCTAACCGGTGCAATTCCTAGCACCCTCGGAAACTTGAGATCTCTCTTGTGGTTGATGCTTGCGAACAATTCCCTAACCGGTCCAATTCCACACGAATTGGGAAATTGTAGTAGCTTGCTGTGGCTTAATCTCGCGAATAACAAGCTCTCCGGGCCAATCCCATCTCAGCTAACAATGATTGGGAGAAATGCTAAGCCAACATTCGACATGAATAATCGGGACAATGATCAGATCGTAGCGGGATCAAGCGAATGCTTAGCGATGAGGAGATGGATTCCTGCAGATTACCCACCATTCAATTTCGTGTACACGATCCTCACTAGGAAGAGTTGTCGAAGCATATGGGACCGGCTGCTTAAAGGAAACGGCCTATTTCCCATATGTGTAGCTGGCTCTTCTGTACGGACGCTTCAAATCTCGGGTTACATTCAACTGAGTGGGAATCAGATTTCGGGTCAGCTCCCTGCAGATATTGGTACAATGCACAGTTTTAGCATGATAAATTTAGGTGTCAATAGATTCCAAGGCGAACTCCCTGCAAATATTGGTCAGTTGCCACTTGTAGTGTTCAACATCAGCAGGAACAATTTTTCGGGTCAAATTCCGGAGGAGATTGGGAATATTAAGTGCATGCAGAATCTTGATATGTCATATAACAATTTTTCTGGCACATTTCCTGTGAGCCTGAACGACTTGAATGAGCTAAGTAATTTCAACATTTCGTACAATCCGCTCATTTCCGGCACAATTCCTTCGACCGGGCAGTTGGCAACGTTCGAGAAAGCGTCCTATCTCGGTAACCCCCTCCTGCAGCTTCCCAAATTCATCGACAACTCCACGTATCCAGAAAACAAAATGAATGGGAATCCAACAAAGCCTACAAAATCTGCTGCATATATGGTGGTCTTCGGTTTGCTAATTGCTCTCTTAATCTGTGGAGTTTTGTCACTTGTAGCATGGTTATTCAGAAAGAGCCGGCCGCAGCCCCCAGGATGTTTGTTGCAGGACATCAAATACCGGCATGACTTGGCCTCGAGCTCTAGCAGTTCATCCCCGTGGCTGTCAGATATTGTTAAGATCATTCGTTTGGACAAAATGGCTTTTACACATGCTGACATTTTGAAAGCTACACGCAATTTTTCGGAAGATAGGATTATAGGGAGTGGAGGGTTTGGGACAGTGTACCAAGGAGTACTGCCGGATGGGAGAGTAGTAGCGGTGAAGAAGCTTCAAAGAGAAGGGCTCGAAGGTGAAAGGGAGTTCCAAGCTGAAATGGAGGTTCTTAGTGGGAATGGCATTGGCTGGCCTCACCCGAACCTTGTAACTCTTCATGGCTGGTGCCTTTATGGATCCGAAAAAATACTAGTCTACGAGTACATGGAAGGCGGGACCTTGGAGGATTTGATATCTGATAGAGTGAGACTGACATGGCGAAGGCGAGTTGATGTGGCAGTTGATGTGGCTCGAGCCTTGATGTTTCTACACCACGAGTGCTTCCCTGCCATCGTGCACCGCGATGTGAAGGCGAGCAATGTGCTGCTAGATAAGGATGGAAAGGCGAGAGTTACAGATTTCGGGCTTGCTAGAATAGTCGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCGGGGACTGTTGGTTATGTCGCTCCCGAATATGGGCAGACATGGCAAGCCACGACGAAAGGAGATGTGTACAGCTACGGAGTTCTGGCAATGGAATTGGCGACCGGAAGGAGAGCGGTGGATGGAGGAGAGGAGTGCCTTGTGGAATGGGCAAGGAGGGTAATGGGAAATGGACGGCAAGGATTCAACCAGTCTGTGATACCGGTTATGCTCATGGGGTCCGGGCTGGCCGATGGGGCGGAGGAGATGTGCGAGCTGCTTAAGGTGGGCATAAAGTGCACGGCGGAGGCACCGCAGTCAAGGCCAAACATGAAGGAGGTTCTAGCTATGCTACTCAAGATATATAAAGATGAAAACATATATAGTTTACAAAGTTATTAA

Protein Analysis

1098

Amino Acids

120.78

Weight (kDa)

6.98

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 38 - 81 4.3e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 103 - 143 3.7e-08 Leucine rich repeat
LRR_8 PF13855 419 - 478 1.9e-08 Leucine rich repeat
LRR_14 PF23598 571 - 671 7e-06 Leucine-rich repeat region
Pkinase PF00069 810 - 1079 5.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 812 - 1081 4.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2625, 3202
AccB7I CCANNNNNTGG 2 cut(s) 1241, 1267
AccBSI CCGCTC 2 cut(s) 2020, 3029
AccI GTMKAC 1 cut(s) 2655
AccII CGCG 3 cut(s) 168, 1421, 2802
AccIII TCCGGA 2 cut(s) 227, 1891
AclI AACGTT 1 cut(s) 2060
AclWI GGATC 5 cut(s) 892, 1537, 1594, 2631, 2644
AcoI YGGCCR 3 cut(s) 439, 2264, 3141
AcsI RAATTY 5 cut(s) 231, 1170, 1789, 1887, 2108
AfiI CCNNNNNNNGG 8 cut(s) 222, 859, 881, 1241, 1267, 2045, 2945, 2946
AflII CTTAAG 1 cut(s) 3173
AgeI ACCGGT 5 cut(s) 293, 1076, 1292, 1364, 3115
AhlI ACTAGT 1 cut(s) 2650
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 4 cut(s) 753, 1121, 1266, 2275
AleI CACNNNNGTG 1 cut(s) 2909
Alw21I GWGCWC 7 cut(s) 276, 373, 638, 2327, 2799, 2906, 3194
Alw26I GTCTC 2 cut(s) 817, 2701
Alw44I GTGCAC 2 cut(s) 2795, 3190
AlwI GGATC 5 cut(s) 892, 1537, 1594, 2631, 2644
AlwNI CAGNNNCTG 2 cut(s) 800, 826
Ama87I CYCGRG 3 cut(s) 1705, 2320, 2751
Aor13HI TCCGGA 2 cut(s) 227, 1891
ApaLI GTGCAC 2 cut(s) 2795, 3190
ApoI RAATTY 5 cut(s) 231, 1170, 1789, 1887, 2108
AsiGI ACCGGT 5 cut(s) 293, 1076, 1292, 1364, 3115
Asp700I GAANNNNTTC 2 cut(s) 572, 1249
AspLEI GCGC 2 cut(s) 204, 719
AspS9I GGNCC 9 cut(s) 225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
AsuC2I CCSGG 5 cut(s) 377, 528, 1438, 2047, 3136
AsuHPI GGTGA 8 cut(s) 283, 308, 568, 665, 1054, 2518, 2549, 2588
AsuII TTCGAA 2 cut(s) 17, 812
AvaI CYCGRG 3 cut(s) 1705, 2320, 2751
AvaII GGWCC 7 cut(s) 225, 1154, 1270, 1367, 1633, 2676, 3132
BaeGI GKGCMC 2 cut(s) 2799, 3194
BaeI ACNNNNGTAYC 4 cut(s) 320, 353, 1063, 1096
BalI TGGCCA 1 cut(s) 441
BamHI GGATCC 1 cut(s) 2636
BanI GGYRCC 2 cut(s) 2625, 3202
BanII GRGCYC 4 cut(s) 276, 638, 2327, 2532
BarI GAAGNNNNNNTAC 2 cut(s) 1680, 1712
BauI CACGAG 1 cut(s) 2774
BbsI GAAGAC 1 cut(s) 2185
Bbv12I GWGCWC 7 cut(s) 276, 373, 638, 2327, 2799, 2906, 3194
BbvCI CCTCAGC 1 cut(s) 1133
BccI CCATC 8 cut(s) 431, 1456, 1551, 2483, 2798, 2831, 3029, 3140
BceAI ACGGC 6 cut(s) 249, 520, 699, 1669, 3104, 3210
BcgI CGANNNNNNTGC 6 cut(s) 792, 826, 1805, 1839, 2803, 2837
BciT130I CCWGG 4 cut(s) 755, 1123, 1268, 2277
BciVI GTATCC 3 cut(s) 706, 1094, 2139
BclI TGATCA 4 cut(s) 69, 75, 828, 1513
BcnI CCSGG 5 cut(s) 377, 528, 1438, 2047, 3136
BcoDI GTCTC 2 cut(s) 817, 2701
BcuI ACTAGT 1 cut(s) 2650
BfaI CTAG 9 cut(s) 545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
BfmI CTRYAG 6 cut(s) 152, 801, 899, 1566, 1752, 2096
BfoI RGCGCY 1 cut(s) 720
BfrI CTTAAG 1 cut(s) 3173
BfuI GTATCC 3 cut(s) 706, 1094, 2139
BglI GCCNNNNNGGC 1 cut(s) 3149
BglII AGATCT 1 cut(s) 1325
BlpI GCTNAGC 4 cut(s) 134, 275, 1479, 1543
BmcAI AGTACT 1 cut(s) 2481
Bme1390I CCNGG 9 cut(s) 377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
Bme18I GGWCC 7 cut(s) 225, 1154, 1270, 1367, 1633, 2676, 3132
BmeT110I CYCGRG 3 cut(s) 1705, 2320, 2751
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 9 cut(s) 225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
BmiI GGNNCC 9 cut(s) 197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
BmrFI CCNGG 9 cut(s) 377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
BmsI GCATC 2 cut(s) 1332, 2872
BoxI GACNNNNGTC 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 2185
BplI GAGNNNNNCTC 2 cut(s) 2514, 2546
Bpu10I CCTNAGC 1 cut(s) 1133
Bpu1102I GCTNAGC 4 cut(s) 134, 275, 1479, 1543
Bpu14I TTCGAA 2 cut(s) 17, 812
BpuEI CTTGAG 4 cut(s) 406, 1088, 1342, 3233
BpuMI CCSGG 5 cut(s) 377, 528, 1438, 2047, 3136
BsaAI YACGTR 1 cut(s) 2127
BsaBI GATNNNNATC 1 cut(s) 74
BsaJI CCNNGG 8 cut(s) 175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BsaWI WCCGGW 9 cut(s) 227, 293, 578, 1076, 1292, 1364, 1891, 3017, 3115
BsaXI ACNNNNNCTCC 4 cut(s) 1418, 1448, 3146, 3176
Bsc4I CCNNNNNNNGG 8 cut(s) 222, 859, 881, 1241, 1267, 2045, 2945, 2946
Bse118I RCCGGY 8 cut(s) 293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
Bse1I ACTGG 3 cut(s) 28, 1163, 3103
Bse3DI GCAATG 3 cut(s) 250, 2824, 3009
Bse8I GATNNNNATC 1 cut(s) 74
BseAI TCCGGA 2 cut(s) 227, 1891
BseBI CCWGG 4 cut(s) 755, 1123, 1268, 2277
BseDI CCNNGG 8 cut(s) 175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BseGI GGATG 5 cut(s) 2285, 2336, 2494, 2842, 3040
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 8 cut(s) 222, 859, 881, 1241, 1267, 2045, 2945, 2946
BseMI GCAATG 3 cut(s) 250, 2824, 3009
BseMII CTCAG 7 cut(s) 125, 289, 418, 867, 1147, 1466, 1712
BseNI ACTGG 3 cut(s) 28, 1163, 3103
BseRI GAGGAG 5 cut(s) 1567, 1910, 3054, 3059, 3170
BseSI GKGCMC 2 cut(s) 2799, 3194
BseX3I CGGCCG 1 cut(s) 2264
BsgI GTGCAG 2 cut(s) 813, 1043
Bsh1236I CGCG 3 cut(s) 168, 1421, 2802
Bsh1285I CGRYCG 3 cut(s) 2046, 2267, 3018
BshNI GGYRCC 2 cut(s) 2625, 3202
BshTI ACCGGT 5 cut(s) 293, 1076, 1292, 1364, 3115
BsiEI CGRYCG 3 cut(s) 2046, 2267, 3018
BsiHKAI GWGCWC 7 cut(s) 276, 373, 638, 2327, 2799, 2906, 3194
BsiHKCI CYCGRG 3 cut(s) 1705, 2320, 2751
BslFI GGGAC 6 cut(s) 909, 1520, 1646, 2476, 2689, 2932
BslI CCNNNNNNNGG 8 cut(s) 222, 859, 881, 1241, 1267, 2045, 2945, 2946
BsmAI GTCTC 2 cut(s) 817, 2701
BsmFI GGGAC 6 cut(s) 909, 1520, 1646, 2476, 2689, 2932
BsmI GAATGC 2 cut(s) 611, 1544
BsoBI CYCGRG 3 cut(s) 1705, 2320, 2751
Bsp119I TTCGAA 2 cut(s) 17, 812
Bsp1286I GDGCHC 8 cut(s) 276, 373, 638, 2327, 2532, 2799, 2906, 3194
Bsp13I TCCGGA 2 cut(s) 227, 1891
Bsp1407I TGTACA 2 cut(s) 1593, 2982
Bsp1720I GCTNAGC 4 cut(s) 134, 275, 1479, 1543
Bsp68I TCGCGA 1 cut(s) 1421
BspCNI CTCAG 7 cut(s) 126, 288, 417, 868, 1146, 1465, 1713
BspEI TCCGGA 2 cut(s) 227, 1891
BspFNI CGCG 3 cut(s) 168, 1421, 2802
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 9 cut(s) 197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
BspMAI CTGCAG 6 cut(s) 156, 805, 903, 1570, 1756, 2100
BspPI GGATC 5 cut(s) 892, 1537, 1594, 2631, 2644
BspQI GCTCTTC 1 cut(s) 1687
BspT104I TTCGAA 2 cut(s) 17, 812
BspT107I GGYRCC 2 cut(s) 2625, 3202
BspTI CTTAAG 1 cut(s) 3173
BsrBI CCGCTC 2 cut(s) 2020, 3029
BsrDI GCAATG 3 cut(s) 250, 2824, 3009
BsrFI RCCGGY 8 cut(s) 293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
BsrGI TGTACA 2 cut(s) 1593, 2982
BsrI ACTGG 3 cut(s) 28, 1163, 3103
BssAI RCCGGY 8 cut(s) 293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
BssECI CCNNGG 8 cut(s) 175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BssSI CACGAG 1 cut(s) 2774
BssT1I CCWWGG 4 cut(s) 175, 1810, 2473, 2679
Bst2BI CACGAG 1 cut(s) 2774
Bst2UI CCWGG 4 cut(s) 755, 1123, 1268, 2277
Bst4CI ACNGT 5 cut(s) 490, 926, 1775, 2467, 2923
Bst6I CTCTTC 3 cut(s) 1607, 1687, 2619
BstAFI CTTAAG 1 cut(s) 3173
BstAPI GCANNNNNTGC 3 cut(s) 216, 800, 3189
BstAUI TGTACA 2 cut(s) 1593, 2982
BstBAI YACGTR 1 cut(s) 2127
BstBI TTCGAA 2 cut(s) 17, 812
BstDSI CCRYGG 1 cut(s) 2342
BstEII GGTNACC 1 cut(s) 2084
BstF5I GGATG 5 cut(s) 2285, 2336, 2494, 2842, 3040
BstFNI CGCG 3 cut(s) 168, 1421, 2802
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 2 cut(s) 204, 719
BstMAI GTCTC 2 cut(s) 817, 2701
BstMCI CGRYCG 3 cut(s) 2046, 2267, 3018
BstNI CCWGG 4 cut(s) 755, 1123, 1268, 2277
BstNSI RCATGY 2 cut(s) 1921, 2399
BstPAI GACNNNNGTC 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 2084
BstSCI CCNGG 9 cut(s) 375, 526, 753, 1121, 1266, 1436, 2045, 2275, 3134
BstSFI CTRYAG 6 cut(s) 152, 801, 899, 1566, 1752, 2096
BstSLI GKGCMC 2 cut(s) 2799, 3194
BstUI CGCG 3 cut(s) 168, 1421, 2802
BstV2I GAAGAC 1 cut(s) 2185
BstX2I RGATCY 2 cut(s) 1325, 2636
BstXI CCANNNNNNTGG 1 cut(s) 1094
BstYI RGATCY 2 cut(s) 1325, 2636
BstZI CGGCCG 1 cut(s) 2264
BsuI GTATCC 3 cut(s) 706, 1094, 2139
BtgI CCRYGG 1 cut(s) 2342
BtgZI GCGATG 2 cut(s) 1562, 2817
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 5 cut(s) 2285, 2336, 2494, 2842, 3040
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 3 cut(s) 197, 718, 2472
BtuMI TCGCGA 1 cut(s) 1421
CaiI CAGNNNCTG 2 cut(s) 800, 826
CciI TCATGA 1 cut(s) 72
CfoI GCGC 2 cut(s) 204, 719
Cfr10I RCCGGY 8 cut(s) 293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
Cfr13I GGNCC 9 cut(s) 225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
CseI GACGC 2 cut(s) 1702, 2061
CspAI ACCGGT 5 cut(s) 293, 1076, 1292, 1364, 3115
CspCI CAANNNNNGTGG 2 cut(s) 1365, 1400
EaeI YGGCCR 3 cut(s) 439, 2264, 3141
EagI CGGCCG 1 cut(s) 2264
Eam1104I CTCTTC 3 cut(s) 1607, 1687, 2619
EarI CTCTTC 3 cut(s) 1607, 1687, 2619
EciI GGCGGA 2 cut(s) 3167, 3212
Ecl136II GAGCTC 3 cut(s) 274, 636, 2325
EclXI CGGCCG 1 cut(s) 2264
Eco130I CCWWGG 4 cut(s) 175, 1810, 2473, 2679
Eco147I AGGCCT 1 cut(s) 905
Eco24I GRGCYC 4 cut(s) 276, 638, 2327, 2532
Eco32I GATATC 1 cut(s) 2694
Eco47I GGWCC 7 cut(s) 225, 1154, 1270, 1367, 1633, 2676, 3132
Eco52I CGGCCG 1 cut(s) 2264
Eco53kI GAGCTC 3 cut(s) 274, 636, 2325
Eco88I CYCGRG 3 cut(s) 1705, 2320, 2751
Eco91I GGTNACC 1 cut(s) 2084
EcoICRI GAGCTC 3 cut(s) 274, 636, 2325
EcoO109I RGGNCCY 2 cut(s) 1154, 2676
EcoO65I GGTNACC 1 cut(s) 2084
EcoRII CCWGG 4 cut(s) 753, 1121, 1266, 2275
EcoRV GATATC 1 cut(s) 2694
EcoT14I CCWWGG 4 cut(s) 175, 1810, 2473, 2679
EcoT38I GRGCYC 4 cut(s) 276, 638, 2327, 2532
ErhI CCWWGG 4 cut(s) 175, 1810, 2473, 2679
FalI AAGNNNNNCTT 4 cut(s) 506, 538, 752, 784
FaqI GGGAC 6 cut(s) 909, 1520, 1646, 2476, 2689, 2932
FauI CCCGC 4 cut(s) 879, 1519, 2666, 2908
FauNDI CATATG 2 cut(s) 1628, 1667
FbaI TGATCA 4 cut(s) 69, 75, 828, 1513
FblI GTMKAC 1 cut(s) 2655
FokI GGATG 5 cut(s) 2292, 2323, 2501, 2849, 3047
FriOI GRGCYC 4 cut(s) 276, 638, 2327, 2532
FspBI CTAG 9 cut(s) 545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
GlaI GCGC 2 cut(s) 203, 718
HaeII RGCGCY 1 cut(s) 720
HgaI GACGC 2 cut(s) 1702, 2061
HhaI GCGC 2 cut(s) 204, 719
Hin6I GCGC 2 cut(s) 202, 717
HinP1I GCGC 2 cut(s) 202, 717
HindIII AAGCTT 1 cut(s) 2513
HphI GGTGA 8 cut(s) 283, 308, 568, 665, 1054, 2518, 2549, 2588
Hpy99I CGWCG 1 cut(s) 2971
HpyCH4III ACNGT 5 cut(s) 490, 926, 1775, 2467, 2923
HpyCH4IV ACGT 3 cut(s) 66, 2060, 2126
HpySE526I ACGT 3 cut(s) 66, 2060, 2126
HspAI GCGC 2 cut(s) 202, 717
Kpn2I TCCGGA 2 cut(s) 227, 1891
KroI GCCGGC 1 cut(s) 2262
KroNI GCCGGC 1 cut(s) 2264
Ksp22I TGATCA 4 cut(s) 69, 75, 828, 1513
LguI GCTCTTC 1 cut(s) 1687
LmnI GCTCC 3 cut(s) 368, 1752, 2941
LweI GCATC 2 cut(s) 1332, 2872
MaeI CTAG 9 cut(s) 545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
MaeII ACGT 3 cut(s) 66, 2060, 2126
MaeIII GTNAC 8 cut(s) 420, 539, 917, 1709, 2084, 2232, 2608, 2851
MbiI CCGCTC 2 cut(s) 2020, 3029
MboII GAAGA 8 cut(s) 364, 526, 1624, 1674, 2185, 2444, 2521, 2606
MflI RGATCY 2 cut(s) 1325, 2636
MhlI GDGCHC 8 cut(s) 276, 373, 638, 2327, 2532, 2799, 2906, 3194
MlsI TGGCCA 1 cut(s) 441
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 4 cut(s) 79, 450, 744, 2180
Mox20I TGGCCA 1 cut(s) 441
MroI TCCGGA 2 cut(s) 227, 1891
MroNI GCCGGC 1 cut(s) 2262
MroXI GAANNNNTTC 2 cut(s) 572, 1249
MscI TGGCCA 1 cut(s) 441
MseI TTAA 9 cut(s) 612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
MslI CAYNNNNRTG 3 cut(s) 2341, 2802, 2909
Msp20I TGGCCA 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 445
MspCI CTTAAG 1 cut(s) 3173
MspR9I CCNGG 9 cut(s) 377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
Mva1269I GAATGC 2 cut(s) 611, 1544
MvaI CCWGG 4 cut(s) 755, 1123, 1268, 2277
MvnI CGCG 3 cut(s) 168, 1421, 2802
NaeI GCCGGC 1 cut(s) 2264
NciI CCSGG 5 cut(s) 377, 528, 1438, 2047, 3136
NdeI CATATG 2 cut(s) 1628, 1667
NgoMIV GCCGGC 1 cut(s) 2262
NlaIV GGNNCC 9 cut(s) 197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
NmuCI GTSAC 2 cut(s) 420, 2232
NruI TCGCGA 1 cut(s) 1421
NspI RCATGY 2 cut(s) 1921, 2399
NspV TTCGAA 2 cut(s) 17, 812
OliI CACNNNNGTG 1 cut(s) 2909
PaeI GCATGC 1 cut(s) 1921
PaeR7I CTCGAG 2 cut(s) 2320, 2751
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 905
PciSI GCTCTTC 1 cut(s) 1687
PcsI WCGNNNNNNNCGW 1 cut(s) 2799
PctI GAATGC 2 cut(s) 611, 1544
PdiI GCCGGC 1 cut(s) 2264
PdmI GAANNNNTTC 2 cut(s) 572, 1249
PflMI CCANNNNNTGG 2 cut(s) 1241, 1267
PfoI TCCNGGA 2 cut(s) 375, 526
PinAI ACCGGT 5 cut(s) 293, 1076, 1292, 1364, 3115
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
Ppu21I YACGTR 1 cut(s) 2127
PpuMI RGGWCCY 2 cut(s) 1154, 2676
PshAI GACNNNNGTC 1 cut(s) 387
Psp124BI GAGCTC 3 cut(s) 276, 638, 2327
Psp1406I AACGTT 1 cut(s) 2060
Psp5II RGGWCCY 2 cut(s) 1154, 2676
Psp6I CCWGG 4 cut(s) 753, 1121, 1266, 2275
PspEI GGTNACC 1 cut(s) 2084
PspGI CCWGG 4 cut(s) 753, 1121, 1266, 2275
PspN4I GGNNCC 9 cut(s) 197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
PspPI GGNCC 9 cut(s) 225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
PspPPI RGGWCCY 2 cut(s) 1154, 2676
PspXI VCTCGAGB 2 cut(s) 2320, 2751
PstI CTGCAG 6 cut(s) 156, 805, 903, 1570, 1756, 2100
PstNI CAGNNNCTG 2 cut(s) 800, 826
PsuI RGATCY 2 cut(s) 1325, 2636
PvuII CAGCTG 1 cut(s) 445
RruI TCGCGA 1 cut(s) 1421
RseI CAYNNNNRTG 3 cut(s) 2341, 2802, 2909
SacI GAGCTC 3 cut(s) 276, 638, 2327
SapI GCTCTTC 1 cut(s) 1687
SaqAI TTAA 9 cut(s) 612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
Sau96I GGNCC 9 cut(s) 225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
ScaI AGTACT 1 cut(s) 2481
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 9 cut(s) 377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
SduI GDGCHC 8 cut(s) 276, 373, 638, 2327, 2532, 2799, 2906, 3194
SfaNI GCATC 2 cut(s) 1332, 2872
SfcI CTRYAG 6 cut(s) 152, 801, 899, 1566, 1752, 2096
Sfr274I CTCGAG 2 cut(s) 2320, 2751
SfuI TTCGAA 2 cut(s) 17, 812
SgrAI CRCCGGYG 2 cut(s) 293, 1076
SinI GGWCC 7 cut(s) 225, 1154, 1270, 1367, 1633, 2676, 3132
SlaI CTCGAG 2 cut(s) 2320, 2751
SmiMI CAYNNNNRTG 3 cut(s) 2341, 2802, 2909
SmlI CTYRAG 7 cut(s) 385, 1103, 1321, 2320, 2751, 3173, 3248
SmoI CTYRAG 7 cut(s) 385, 1103, 1321, 2320, 2751, 3173, 3248
SpeI ACTAGT 1 cut(s) 2650
SphI GCATGC 1 cut(s) 1921
SseBI AGGCCT 1 cut(s) 905
SspI AATATT 3 cut(s) 51, 1831, 1909
SspMI CTAG 9 cut(s) 545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
SstI GAGCTC 3 cut(s) 276, 638, 2327
StuI AGGCCT 1 cut(s) 905
StyD4I CCNGG 9 cut(s) 375, 526, 753, 1121, 1266, 1436, 2045, 2275, 3134
StyI CCWWGG 4 cut(s) 175, 1810, 2473, 2679
TaaI ACNGT 5 cut(s) 490, 926, 1775, 2467, 2923
TaiI ACGT 3 cut(s) 69, 2063, 2129
TatI WGTACW 5 cut(s) 3, 1593, 2479, 2661, 2982
TauI GCSGC 2 cut(s) 1008, 2269
Tru1I TTAA 9 cut(s) 612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
Tru9I TTAA 9 cut(s) 612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
TscAI CASTG 3 cut(s) 197, 718, 2472
TseFI GTSAC 2 cut(s) 420, 2232
Tsp45I GTSAC 2 cut(s) 420, 2232
TspGWI ACGGA 4 cut(s) 79, 1236, 1705, 3006
TspRI CASTG 3 cut(s) 197, 718, 2472
Van91I CCANNNNNTGG 2 cut(s) 1241, 1267
Vha464I CTTAAG 1 cut(s) 3173
VneI GTGCAC 2 cut(s) 2795, 3190
VpaK11BI GGWCC 7 cut(s) 225, 1154, 1270, 1367, 1633, 2676, 3132
XapI RAATTY 5 cut(s) 231, 1170, 1789, 1887, 2108
XbaI TCTAGA 1 cut(s) 932
XceI RCATGY 2 cut(s) 1921, 2399
XhoI CTCGAG 2 cut(s) 2320, 2751
XmiI GTMKAC 1 cut(s) 2655
XmnI GAANNNNTTC 2 cut(s) 572, 1249
XspI CTAG 9 cut(s) 545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
ZrmI AGTACT 1 cut(s) 2481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.