pycom06g20830
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004135
Physical Location & Seq
Forward (+)
23731379 .. 23733980
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGCTTCACGAAAAAGGGCTTTCATCAGAAGCAGCTGCAGGCGGTAGAAGCTGCTTAGCTTTGAATCCGATTCCTGATCTTTCACTCCACATAAGCCCTCCAAACACAAACATAAACAGTGCTCCCTCCTCCATTTGCACCGACCGAGTGACGCCCGATCACGACTCCACCAGCTGCTTCGACATATGGCGAAGAGACACCGACGAGGACTACACCAACGGCGCTGCCCTAGCCAACAAATCCCACAGCGACAGCTGTATTAGGGCCAGCTACACCAGTTCTTCACCAGTAGCTGCTGCCGCCGACACTGAGCTTTCCCTCGCGAACCCCTCGGCCTTAGAGGCCGAAAGCGCCTGGAAGAAGAACTATTTCGGCGGTGGAGACGGTTACAATGACGAAGCGAAGAATAGCGGTAATAGTGTAACTAGTAGTAATGGGATTTCGATGCTTGAGAGACTCAAACCCATCAAAGGGATTCCCGTTTACAGTACTAGTAGTACTAATTGCTCCTTTCCTCCTTCGTTTTCCCCTAATCCTGTGTACCAAACGGCGCCTTATCTGTCTAATTCCTTCAGACCGGCGGCAGCTGCTCGGAATTGTAGTGGCAGGGGAATGGGAATGGGATTGGGAGGGTTTAACGGGATAACGATGGACAGTCTAAGCCTAGGAGTAGGAGGAGTGCAACAACAACAGCAGCAGCATCAACGACAACACCATTTTCCGTCGTATAATTTGAATCCTTATTATCCTCAGCAGCTGCAGCAGCATCACCGTGTTGGAGGAGGGGGAAATTACAACGCCGACTTGTCGTCGAATGGTTTCACCATGAGATCGAAATTTACGCCGCCGAAGCTCATAGGTCAAAATATTAATAAGAGGAACATGAGGGCTCCGAGGATGAGATGGACTACTTCTCTTCATGCTCGTTTTGTTCATGCTGTCGAGCTCCTTGGTGGTCATGAAAGGGCTACTCCGAAGTCAGTGTTGGAGCTCATGGACGTCAAGGATCTCACACTTGCTCATGTTAAGAGCCATTTGCAGATGTATAGAACTGTTAAGAACACTGACAAACCTCTAGCTTCCTCAGATGGATCTGGGGATGAAGATCGATCCTTTTGTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

375

Amino Acids

40.48

Weight (kDa)

8.41

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 299 - 350 2.1e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1002
AccB1I GGYRCC 1 cut(s) 550
AccII CGCG 1 cut(s) 323
AciI CCGC 6 cut(s) 42, 300, 375, 411, 581, 845
AclWI GGATC 3 cut(s) 1014, 1099, 1104
AcsI RAATTY 1 cut(s) 836
AcuI CTGAAG 1 cut(s) 556
AcyI GRCGYC 3 cut(s) 152, 551, 999
AfaI GTAC 3 cut(s) 490, 499, 542
AfiI CCNNNNNNNGG 2 cut(s) 470, 471
AgsI TTSAA 2 cut(s) 64, 736
AhdI GACNNNNNGTC 1 cut(s) 808
AhlI ACTAGT 2 cut(s) 425, 491
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
Alw21I GWGCWC 3 cut(s) 124, 948, 993
Alw26I GTCTC 3 cut(s) 189, 375, 448
AlwI GGATC 3 cut(s) 1014, 1099, 1104
AlwNI CAGNNNCTG 2 cut(s) 293, 757
AoxI GGCC 3 cut(s) 264, 333, 342
ApoI RAATTY 1 cut(s) 836
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 2 cut(s) 368, 818
AspA2I CCTAGG 1 cut(s) 664
AspLEI GCGC 3 cut(s) 224, 353, 553
AspS9I GGNCC 1 cut(s) 264
AsuHPI GGTGA 3 cut(s) 276, 761, 814
AvrII CCTAGG 1 cut(s) 664
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 3 cut(s) 892, 948, 993
Bbv12I GWGCWC 3 cut(s) 124, 948, 993
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 4 cut(s) 473, 643, 897, 1082
BceAI ACGGC 2 cut(s) 235, 564
BciT130I CCWGG 1 cut(s) 355
BcoDI GTCTC 3 cut(s) 189, 375, 448
BcuI ACTAGT 2 cut(s) 425, 491
BfaI CTAG 5 cut(s) 230, 426, 492, 665, 1076
BfmI CTRYAG 2 cut(s) 36, 758
BfoI RGCGCY 3 cut(s) 225, 354, 554
BglI GCCNNNNNGGC 1 cut(s) 341
BlnI CCTAGG 1 cut(s) 664
BlpI GCTNAGC 1 cut(s) 55
BmcAI AGTACT 2 cut(s) 490, 499
Bme1390I CCNGG 1 cut(s) 355
BmeRI GACNNNNNGTC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 552, 891
BmrFI CCNGG 1 cut(s) 355
BmsI GCATC 3 cut(s) 435, 709, 775
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 470
Bsa29I ATCGAT 1 cut(s) 1108
BsaBI GATNNNNATC 1 cut(s) 1104
BsaHI GRCGYC 3 cut(s) 152, 551, 999
BsaJI CCNNGG 4 cut(s) 330, 664, 893, 949
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 471
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 276, 287
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 1108
BseDI CCNNGG 4 cut(s) 330, 664, 893, 949
BseGI GGATG 2 cut(s) 903, 1105
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 2 cut(s) 470, 471
BseMII CTCAG 3 cut(s) 300, 764, 1098
BseNI ACTGG 2 cut(s) 276, 287
BseRI GAGGAG 3 cut(s) 118, 690, 795
Bsh1236I CGCG 1 cut(s) 323
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 266, 335, 344
BshNI GGYRCC 1 cut(s) 550
BshVI ATCGAT 1 cut(s) 1108
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 3 cut(s) 124, 948, 993
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 2 cut(s) 470, 471
BsmAI GTCTC 3 cut(s) 189, 375, 448
BsmBI CGTCTC 1 cut(s) 375
BsnI GGCC 3 cut(s) 266, 335, 344
Bsp1286I GDGCHC 4 cut(s) 124, 892, 948, 993
Bsp143I GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp68I TCGCGA 1 cut(s) 323
BspACI CCGC 6 cut(s) 42, 300, 375, 411, 581, 845
BspANI GGCC 3 cut(s) 266, 335, 344
BspCNI CTCAG 3 cut(s) 301, 763, 1097
BspDI ATCGAT 1 cut(s) 1108
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 2 cut(s) 552, 891
BspMAI CTGCAG 2 cut(s) 40, 762
BspPI GGATC 3 cut(s) 1014, 1099, 1104
BspT107I GGYRCC 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 2 cut(s) 276, 287
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 330, 664, 893, 949
BssMI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
BssNI GRCGYC 3 cut(s) 152, 551, 999
BssT1I CCWWGG 2 cut(s) 664, 949
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
Bst6I CTCTTC 2 cut(s) 187, 921
BstACI GRCGYC 3 cut(s) 152, 551, 999
BstC8I GCNNGC 2 cut(s) 40, 268
BstDEI CTNAG 6 cut(s) 55, 309, 337, 659, 750, 1084
BstF5I GGATG 2 cut(s) 903, 1105
BstFNI CGCG 1 cut(s) 323
BstH2I RGCGCY 3 cut(s) 225, 354, 554
BstHHI GCGC 3 cut(s) 224, 353, 553
BstKTI GATC 7 cut(s) 79, 160, 833, 1009, 1094, 1108, 1112
BstMAI GTCTC 3 cut(s) 189, 375, 448
BstMBI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 9 cut(s) 48, 230, 299, 341, 350, 587, 760, 763, 850
BstNI CCWGG 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 36, 758
BstUI CGCG 1 cut(s) 323
BstX2I RGATCY 2 cut(s) 1006, 1091
BstYI RGATCY 2 cut(s) 1006, 1091
Bsu15I ATCGAT 1 cut(s) 1108
BsuRI GGCC 3 cut(s) 266, 335, 344
BsuTUI ATCGAT 1 cut(s) 1108
BtsCI GGATG 2 cut(s) 903, 1105
BtsIMutI CAGTG 4 cut(s) 124, 306, 987, 1062
BtuMI TCGCGA 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 40, 268
CaiI CAGNNNCTG 2 cut(s) 293, 757
CciI TCATGA 1 cut(s) 958
CfoI GCGC 3 cut(s) 224, 353, 553
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 1108
CseI GACGC 1 cut(s) 160
Csp6I GTAC 3 cut(s) 489, 498, 541
CviAII CATG 7 cut(s) 826, 883, 920, 935, 959, 994, 1022
CviQI GTAC 3 cut(s) 489, 498, 541
DdeI CTNAG 6 cut(s) 55, 309, 337, 659, 750, 1084
DinI GGCGCC 1 cut(s) 552
DpnI GATC 7 cut(s) 78, 159, 832, 1008, 1093, 1107, 1111
DpnII GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
DriI GACNNNNNGTC 1 cut(s) 808
Eam1104I CTCTTC 2 cut(s) 187, 921
Eam1105I GACNNNNNGTC 1 cut(s) 808
EarI CTCTTC 2 cut(s) 187, 921
Ecl136II GAGCTC 2 cut(s) 946, 991
Eco130I CCWWGG 2 cut(s) 664, 949
Eco24I GRGCYC 3 cut(s) 892, 948, 993
Eco53kI GAGCTC 2 cut(s) 946, 991
Eco57I CTGAAG 1 cut(s) 556
EcoICRI GAGCTC 2 cut(s) 946, 991
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 2 cut(s) 664, 949
EcoT38I GRGCYC 3 cut(s) 892, 948, 993
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
ErhI CCWWGG 2 cut(s) 664, 949
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
FatI CATG 7 cut(s) 825, 882, 919, 934, 958, 993, 1021
FauNDI CATATG 1 cut(s) 185
FokI GGATG 2 cut(s) 910, 1112
FriOI GRGCYC 3 cut(s) 892, 948, 993
FspBI CTAG 5 cut(s) 230, 426, 492, 665, 1076
GlaI GCGC 3 cut(s) 223, 352, 552
HaeII RGCGCY 3 cut(s) 225, 354, 554
HaeIII GGCC 3 cut(s) 266, 335, 344
HapII CCGG 1 cut(s) 578
HgaI GACGC 1 cut(s) 160
HhaI GCGC 3 cut(s) 224, 353, 553
Hin1I GRCGYC 3 cut(s) 152, 551, 999
Hin1II CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
Hin6I GCGC 3 cut(s) 222, 351, 551
HinP1I GCGC 3 cut(s) 222, 351, 551
HinfI GANTC 6 cut(s) 64, 70, 164, 456, 475, 736
HpaII CCGG 1 cut(s) 578
HphI GGTGA 3 cut(s) 276, 761, 814
Hpy166II GTNNAC 2 cut(s) 484, 541
Hpy188I TCNGA 7 cut(s) 28, 69, 575, 594, 894, 975, 1087
Hpy188III TCNNGA 5 cut(s) 8, 74, 161, 322, 959
Hpy8I GTNNAC 2 cut(s) 484, 541
Hpy99I CGWCG 3 cut(s) 206, 727, 814
HpyAV CCTTC 2 cut(s) 528, 580
HpyCH4III ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
HpyCH4IV ACGT 1 cut(s) 999
HpyCH4V TGCA 5 cut(s) 38, 138, 682, 760, 1039
HpyF10VI GCNNNNNNNGC 9 cut(s) 48, 230, 299, 341, 350, 587, 760, 763, 850
HpyF3I CTNAG 6 cut(s) 55, 309, 337, 659, 750, 1084
HpySE526I ACGT 1 cut(s) 999
Hsp92I GRCGYC 3 cut(s) 152, 551, 999
Hsp92II CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
HspAI GCGC 3 cut(s) 222, 351, 551
KasI GGCGCC 1 cut(s) 550
Kzo9I GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
LmnI GCTCC 5 cut(s) 127, 512, 895, 951, 988
LweI GCATC 3 cut(s) 435, 709, 775
MaeI CTAG 5 cut(s) 230, 426, 492, 665, 1076
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 3 cut(s) 148, 386, 421
MalI GATC 7 cut(s) 78, 159, 832, 1008, 1093, 1107, 1111
MboI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
MboII GAAGA 7 cut(s) 204, 273, 370, 373, 415, 908, 1115
MflI RGATCY 2 cut(s) 1006, 1091
MhlI GDGCHC 4 cut(s) 124, 892, 948, 993
MluCI AATT 6 cut(s) 502, 565, 595, 730, 790, 836
Mly113I GGCGCC 1 cut(s) 551
MlyI GAGTC 2 cut(s) 158, 450
MmeI TCCRAC 2 cut(s) 757, 966
MroXI GAANNNNTTC 2 cut(s) 368, 818
MseI TTAA 4 cut(s) 636, 870, 1026, 1056
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 5 cut(s) 35, 174, 255, 587, 757
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 1 cut(s) 355
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 9 cut(s) 48, 230, 299, 341, 350, 587, 760, 763, 850
NarI GGCGCC 1 cut(s) 551
NdeI CATATG 1 cut(s) 185
NdeII GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
NlaIII CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
NlaIV GGNNCC 2 cut(s) 552, 891
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 148
NruI TCGCGA 1 cut(s) 323
PagI TCATGA 1 cut(s) 958
PdmI GAANNNNTTC 2 cut(s) 368, 818
PfeI GAWTC 4 cut(s) 64, 70, 475, 736
PleI GAGTC 2 cut(s) 158, 450
PluTI GGCGCC 1 cut(s) 554
PpsI GAGTC 2 cut(s) 158, 450
PshBI ATTAAT 1 cut(s) 870
Psp124BI GAGCTC 2 cut(s) 948, 993
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 552, 891
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 2 cut(s) 40, 762
PstNI CAGNNNCTG 2 cut(s) 293, 757
PsuI RGATCY 2 cut(s) 1006, 1091
PvuII CAGCTG 5 cut(s) 35, 174, 255, 587, 757
RruI TCGCGA 1 cut(s) 323
RsaI GTAC 3 cut(s) 490, 499, 542
RsaNI GTAC 3 cut(s) 489, 498, 541
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 2 cut(s) 948, 993
SaqAI TTAA 4 cut(s) 636, 870, 1026, 1056
Sau3AI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1109
Sau96I GGNCC 1 cut(s) 264
ScaI AGTACT 2 cut(s) 490, 499
SchI GAGTC 2 cut(s) 158, 450
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 4 cut(s) 124, 892, 948, 993
SfaNI GCATC 3 cut(s) 435, 709, 775
SfcI CTRYAG 2 cut(s) 36, 758
SfiI GGCCNNNNNGGCC 1 cut(s) 341
SfoI GGCGCC 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SpeI ACTAGT 2 cut(s) 425, 491
Sse9I AATT 6 cut(s) 502, 565, 595, 730, 790, 836
SsiI CCGC 6 cut(s) 42, 300, 375, 411, 581, 845
SspDI GGCGCC 1 cut(s) 550
SspI AATATT 1 cut(s) 868
SspMI CTAG 5 cut(s) 230, 426, 492, 665, 1076
SstI GAGCTC 2 cut(s) 948, 993
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 2 cut(s) 664, 949
TaaI ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 6 cut(s) 180, 443, 812, 833, 942, 1108
TaqII GACCGA 1 cut(s) 159
TasI AATT 6 cut(s) 502, 565, 595, 730, 790, 836
TatI WGTACW 2 cut(s) 488, 497
TauI GCSGC 3 cut(s) 302, 584, 847
TfiI GAWTC 4 cut(s) 64, 70, 475, 736
Tru1I TTAA 4 cut(s) 636, 870, 1026, 1056
Tru9I TTAA 4 cut(s) 636, 870, 1026, 1056
TscAI CASTG 4 cut(s) 124, 313, 987, 1069
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 5 cut(s) 12, 908, 923, 975, 1116
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 4 cut(s) 124, 313, 987, 1069
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 1 cut(s) 836
XmaJI CCTAGG 1 cut(s) 664
XmnI GAANNNNTTC 2 cut(s) 368, 818
XspI CTAG 5 cut(s) 230, 426, 492, 665, 1076
ZraI GACGTC 1 cut(s) 1000
ZrmI AGTACT 2 cut(s) 490, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.