pycom07g12500

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007530
Physical Location & Seq
Forward (+)
13858897 .. 13859958
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12500.1

Sequence Viewer

Length: 876 bp
ATGGCTTCCCTGTTTACCAAGTCTTGTTCTAAAGTTGTCATTACCTTCCTACTGATTCTCCCATTTTTGGTTTCGAGGTCGTTCGCCGGGCGCATTGTGGTTTATTGGGGACAAAATGGAGGGGAGGGCACACTAACAGCAACATGTCACACAGGAAGGTACCGGATTGTAAACATAGCATTCCTCTCACAGTTCGGCAATGGCCAGACCCCTCAAATCAACTTAGCCGGGCACTGTGATCCGGCGTCTAACGGCTGTCAAAAAGTGAGCACCAGCATCAGAAACTGCCAGAACCAAGGCATTAAGGTCCTGCTTTCGATTGGCGGCAGCTTGGAGAGTTACACGCTACCATCTGCAGATGATGCTAGAGGTGTAGCGGACTACATATGGAATAACTTCTTAGGGGGGCAATCAAACTCGAGGCCTCTAGGAGACGCCGTATTGGATGGCGTGGATTTCGATATCGAGAGAGGTGAGATTCAGTACGCTGCACTTGCTAGGAGTGCAGCACCGCAATGTCCGTTTCCTGACCAGAAACTCAATGGTGCACTAACCACTGGGAAGTTGCTCATGGGTTTCCAAAAACAAAACCGTGAGGGAATGGTAAGCCCAAAGCGGACAATATCGTACTACGGTAGTGGAGCTGGCCCGGGAAGTGATCTGCCGCGGGCCCTAATAGTTTTGCAGTCTAGCAACCTATTTATTTTTGGTCTACCTTTAAGAGCAAATTTTCAATGTATTGGAAACACGACTTGGTGTACCGACTGTGTTCTTTTTCAGTGTCCTTGTTTCCAAACATCTTTTGGCATGCTACATCTACTTAAAATTCTAACATATTTTTTTCTGGAGTGGTTGAAGATTTTCATAGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

292

Amino Acids

31.75

Weight (kDa)

8.63

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 31 - 169 2.5e-12 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 712
AccII CGCG 1 cut(s) 667
AciI CCGC 6 cut(s) 324, 377, 512, 616, 665, 667
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 727, 825
AcyI GRCGYC 2 cut(s) 245, 435
AfaI GTAC 4 cut(s) 161, 485, 629, 760
AfiI CCNNNNNNNGG 1 cut(s) 67
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 2 cut(s) 734, 856
AjuI GAANNNNNNNTTGG 2 cut(s) 736, 768
AluBI AGCT 2 cut(s) 330, 644
AluI AGCT 2 cut(s) 330, 644
Alw21I GWGCWC 2 cut(s) 272, 550
Alw26I GTCTC 1 cut(s) 426
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 285
Ama87I CYCGRG 2 cut(s) 418, 649
AoxI GGCC 4 cut(s) 202, 422, 646, 669
ApaI GGGCCC 1 cut(s) 673
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 327, 488, 506
ApoI RAATTY 2 cut(s) 727, 825
Asp700I GAANNNNTTC 2 cut(s) 395, 860
Asp718I GGTACC 1 cut(s) 159
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 4 cut(s) 307, 647, 669, 670
AsuC2I CCSGG 4 cut(s) 88, 229, 650, 651
AsuHPI GGTGA 1 cut(s) 485
AvaI CYCGRG 2 cut(s) 418, 649
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 4 cut(s) 131, 234, 550, 673
BalI TGGCCA 1 cut(s) 204
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 673
Bbv12I GWGCWC 2 cut(s) 272, 550
BbvI GCAGC 3 cut(s) 339, 475, 518
BccI CCATC 2 cut(s) 358, 440
BceAI ACGGC 2 cut(s) 268, 422
BcnI CCSGG 4 cut(s) 88, 229, 650, 651
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 4 cut(s) 366, 428, 498, 690
BfmI CTRYAG 1 cut(s) 354
BisI GCNGC 5 cut(s) 325, 328, 489, 507, 665
BlsI GCNGC 5 cut(s) 326, 329, 490, 508, 666
Bme1390I CCNGG 4 cut(s) 88, 229, 650, 651
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 418, 649
BmgT120I GGNCC 4 cut(s) 307, 647, 669, 670
BmiI GGNNCC 2 cut(s) 161, 671
BmrFI CCNGG 4 cut(s) 88, 229, 650, 651
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 2 cut(s) 285, 352
BmuI ACTGGG 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 866
BpuMI CCSGG 4 cut(s) 88, 229, 650, 651
BsaHI GRCGYC 2 cut(s) 245, 435
BsaJI CCNNGG 3 cut(s) 295, 649, 665
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 562
Bse3DI GCAATG 2 cut(s) 205, 521
BseDI CCNNGG 3 cut(s) 295, 649, 665
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 2 cut(s) 205, 521
BseNI ACTGG 1 cut(s) 562
BseSI GKGCMC 4 cut(s) 131, 234, 550, 673
BseXI GCAGC 3 cut(s) 339, 475, 518
BsgI GTGCAG 2 cut(s) 474, 525
Bsh1236I CGCG 1 cut(s) 667
BshFI GGCC 4 cut(s) 204, 424, 648, 671
BshNI GGYRCC 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 272, 550
BsiHKCI CYCGRG 2 cut(s) 418, 649
BsiSI CCGG 5 cut(s) 87, 163, 228, 242, 650
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 426
BsmBI CGTCTC 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 4 cut(s) 204, 424, 648, 671
BsoBI CYCGRG 2 cut(s) 418, 649
Bsp120I GGGCCC 1 cut(s) 669
Bsp1286I GDGCHC 5 cut(s) 131, 234, 272, 550, 673
Bsp143I GATC 2 cut(s) 238, 658
BspACI CCGC 6 cut(s) 324, 377, 512, 616, 665, 667
BspANI GGCC 4 cut(s) 204, 424, 648, 671
BspFNI CGCG 1 cut(s) 667
BspLI GGNNCC 2 cut(s) 161, 671
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 159
BsrDI GCAATG 2 cut(s) 205, 521
BsrI ACTGG 1 cut(s) 562
BssECI CCNNGG 3 cut(s) 295, 649, 665
BssMI GATC 2 cut(s) 238, 658
BssNI GRCGYC 2 cut(s) 245, 435
BssT1I CCWWGG 1 cut(s) 295
Bst4CI ACNGT 5 cut(s) 192, 236, 593, 635, 767
BstACI GRCGYC 2 cut(s) 245, 435
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 3 cut(s) 646, 669, 809
BstDEI CTNAG 2 cut(s) 223, 400
BstDSI CCRYGG 1 cut(s) 665
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 667
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 2 cut(s) 241, 661
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 2 cut(s) 238, 658
BstMWI GCNNNNNNNGC 3 cut(s) 362, 494, 503
BstNSI RCATGY 2 cut(s) 147, 811
BstSCI CCNGG 4 cut(s) 86, 227, 648, 649
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 4 cut(s) 131, 234, 550, 673
BstUI CGCG 1 cut(s) 667
BstV1I GCAGC 3 cut(s) 339, 475, 518
BsuRI GGCC 4 cut(s) 204, 424, 648, 671
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 232, 555, 785
Cac8I GCNNGC 3 cut(s) 646, 669, 809
CaiI CAGNNNCTG 1 cut(s) 285
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 4 cut(s) 307, 647, 669, 670
Cfr42I CCGCGG 1 cut(s) 668
Cfr9I CCCGGG 1 cut(s) 649
CseI GACGC 2 cut(s) 234, 443
Csp6I GTAC 4 cut(s) 160, 484, 628, 759
CviAII CATG 3 cut(s) 144, 571, 808
CviQI GTAC 4 cut(s) 160, 484, 628, 759
DdeI CTNAG 2 cut(s) 223, 400
DpnI GATC 2 cut(s) 240, 660
DpnII GATC 2 cut(s) 238, 658
EaeI YGGCCR 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 673
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 307
Eco88I CYCGRG 2 cut(s) 418, 649
EcoO109I RGGNCCY 2 cut(s) 307, 670
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 426
FaeI CATG 3 cut(s) 147, 574, 811
FaiI YATR 8 cut(s) 145, 176, 386, 388, 572, 809, 835, 866
FaqI GGGAC 1 cut(s) 123
FatI CATG 3 cut(s) 143, 570, 807
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 712
Fnu4HI GCNGC 5 cut(s) 325, 328, 489, 507, 665
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 5 cut(s) 325, 328, 489, 507, 665
FspBI CTAG 4 cut(s) 366, 428, 498, 690
GlaI GCGC 1 cut(s) 92
GluI GCNGC 5 cut(s) 325, 328, 489, 507, 665
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 4 cut(s) 204, 424, 648, 671
HapII CCGG 5 cut(s) 87, 163, 228, 242, 650
HgaI GACGC 2 cut(s) 234, 443
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 2 cut(s) 245, 435
Hin1II CATG 3 cut(s) 147, 574, 811
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 2 cut(s) 55, 478
HpaII CCGG 5 cut(s) 87, 163, 228, 242, 650
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 5 cut(s) 15, 172, 548, 713, 759
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 3 cut(s) 466, 527, 845
Hpy8I GTNNAC 5 cut(s) 15, 172, 548, 713, 759
HpyAV CCTTC 2 cut(s) 55, 150
HpyCH4III ACNGT 5 cut(s) 192, 236, 593, 635, 767
HpyCH4V TGCA 5 cut(s) 356, 491, 506, 548, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 494, 503
HpyF3I CTNAG 2 cut(s) 223, 400
Hsp92I GRCGYC 2 cut(s) 245, 435
Hsp92II CATG 3 cut(s) 147, 574, 811
HspAI GCGC 1 cut(s) 91
KpnI GGTACC 1 cut(s) 163
KspI CCGCGG 1 cut(s) 668
Kzo9I GATC 2 cut(s) 238, 658
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 3 cut(s) 339, 475, 518
LweI GCATC 2 cut(s) 285, 352
MaeI CTAG 4 cut(s) 366, 428, 498, 690
MaeIII GTNAC 2 cut(s) 146, 338
MalI GATC 2 cut(s) 240, 660
MboI GATC 2 cut(s) 238, 658
MboII GAAGA 1 cut(s) 868
MhlI GDGCHC 5 cut(s) 131, 234, 272, 550, 673
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 2 cut(s) 727, 825
MluNI TGGCCA 1 cut(s) 204
Mox20I TGGCCA 1 cut(s) 204
MroXI GAANNNNTTC 2 cut(s) 395, 860
MscI TGGCCA 1 cut(s) 204
MseI TTAA 3 cut(s) 303, 719, 822
MslI CAYNNNNRTG 1 cut(s) 514
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 667
MspI CCGG 5 cut(s) 87, 163, 228, 242, 650
MspR9I CCNGG 4 cut(s) 88, 229, 650, 651
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 1 cut(s) 667
MwoI GCNNNNNNNGC 3 cut(s) 362, 494, 503
NciI CCSGG 4 cut(s) 88, 229, 650, 651
NdeI CATATG 1 cut(s) 386
NdeII GATC 2 cut(s) 238, 658
NlaIII CATG 3 cut(s) 147, 574, 811
NlaIV GGNNCC 2 cut(s) 161, 671
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 2 cut(s) 147, 811
PaeI GCATGC 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 418
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 143
PctI GAATGC 1 cut(s) 179
PdmI GAANNNNTTC 2 cut(s) 395, 860
PfeI GAWTC 2 cut(s) 55, 478
PkrI GCNGC 5 cut(s) 326, 329, 490, 508, 666
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 161, 671
PspOMI GGGCCC 1 cut(s) 669
PspPI GGNCC 4 cut(s) 307, 647, 669, 670
PspPPI RGGWCCY 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 418
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 285
RsaI GTAC 4 cut(s) 161, 485, 629, 760
RsaNI GTAC 4 cut(s) 160, 484, 628, 759
RseI CAYNNNNRTG 1 cut(s) 514
SacII CCGCGG 1 cut(s) 668
SaqAI TTAA 3 cut(s) 303, 719, 822
SatI GCNGC 5 cut(s) 325, 328, 489, 507, 665
Sau3AI GATC 2 cut(s) 238, 658
Sau96I GGNCC 4 cut(s) 307, 647, 669, 670
ScrFI CCNGG 4 cut(s) 88, 229, 650, 651
SduI GDGCHC 5 cut(s) 131, 234, 272, 550, 673
SfaNI GCATC 2 cut(s) 285, 352
SfcI CTRYAG 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 418
Sfr303I CCGCGG 1 cut(s) 668
SgrBI CCGCGG 1 cut(s) 668
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 418
SmaI CCCGGG 1 cut(s) 651
SmiMI CAYNNNNRTG 1 cut(s) 514
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SphI GCATGC 1 cut(s) 811
Sse9I AATT 2 cut(s) 727, 825
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 6 cut(s) 324, 377, 512, 616, 665, 667
SspMI CTAG 4 cut(s) 366, 428, 498, 690
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 4 cut(s) 86, 227, 648, 649
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 5 cut(s) 192, 236, 593, 635, 767
TaqI TCGA 5 cut(s) 74, 317, 419, 459, 465
TasI AATT 2 cut(s) 727, 825
TauI GCSGC 2 cut(s) 327, 667
TfiI GAWTC 2 cut(s) 55, 478
Tru1I TTAA 3 cut(s) 303, 719, 822
Tru9I TTAA 3 cut(s) 303, 719, 822
TscAI CASTG 3 cut(s) 239, 562, 785
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 327, 488, 506
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 853
TspGWI ACGGA 1 cut(s) 510
TspMI CCCGGG 1 cut(s) 649
TspRI CASTG 3 cut(s) 239, 562, 785
VneI GTGCAC 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 727, 825
XceI RCATGY 2 cut(s) 147, 811
XcmI CCANNNNNNNNNTGG 2 cut(s) 539, 800
XhoI CTCGAG 1 cut(s) 418
XmaI CCCGGG 1 cut(s) 649
XmiI GTMKAC 1 cut(s) 712
XmnI GAANNNNTTC 2 cut(s) 395, 860
XspI CTAG 4 cut(s) 366, 428, 498, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.