Rmu_sc0002144.1_g000033

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002144.1
OG0007530
Physical Location & Seq
Reverse (-)
145512 .. 146417
906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002144.1_g000033.1.cds

Sequence Viewer

Length: 906 bp
atggctgccatgtccaccgagtcgtgctctcaagctctcactgtcttactagtaatcctccatatctttgtctcaaactcttacgccggatccattgtagtttactggggccaaaatgggggagaaggcacactcacagaagcatgtgacacaggcagatatcgtatcgtaaacatagcattcctctcacagtatggcaatggccaaacacctcagatcaatttagccggtcactgcgatccaagatcaaatggttgcgtaaaagtgagcacagacatcaaaaattgccaaaacaaaggcatcaaggtccttctttccattggcggtgctggtgggaattatggactatcatcagatgccgatgctgggaaagtagctgactacatatggaataatttcttgggaggtcaatcgagctcaaggcctctaggcgacgccgttttggacggggtagatttcgacatcgagaaaggcggaccccactatgatgcgcttgctaggcagttgtctcagtatagccaaagagggaggaaagtgtacttaggagcagcaccgcagtgcccatttcctgaccagtatgtaaatggtgcattgtccactggcttatttgactatgtttgggttcaattctacaacaatccacaatgtgagttcacctctagcaatcccaatgcctttaggaactcttggaataagtggacatcatctatacaagccaatcaattctttgttgggcttccagcttctacagcagctgctacgaatggttatgtgcctacagctgatctgataaaccaagtgttgccttttgttaagaagtctcccaaatatggaggtatcatgctctatgataggtttggagatgctaacactggatacagttctaaagttataggcagtgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

301

Amino Acids

32.32

Weight (kDa)

6.8

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 324, 474, 554
AclWI GGATC 3 cut(s) 84, 97, 233
AcoI YGGCCR 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 3 cut(s) 118, 366, 830
AgsI TTSAA 1 cut(s) 626
AhlI ACTAGT 1 cut(s) 49
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AleI CACNNNNGTG 1 cut(s) 556
AluBI AGCT 6 cut(s) 35, 377, 417, 743, 755, 782
AluI AGCT 6 cut(s) 35, 377, 417, 743, 755, 782
Alw21I GWGCWC 3 cut(s) 29, 272, 419
Alw26I GTCTC 3 cut(s) 76, 513, 825
AlwI GGATC 3 cut(s) 84, 97, 233
AlwNI CAGNNNCTG 1 cut(s) 755
AoxI GGCC 3 cut(s) 109, 202, 422
ApeKI GCWGC 4 cut(s) 5, 548, 752, 755
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 3 cut(s) 109, 307, 476
AsuHPI GGTGA 1 cut(s) 646
AvaII GGWCC 2 cut(s) 307, 476
BaeGI GKGCMC 1 cut(s) 563
BalI TGGCCA 1 cut(s) 204
BamHI GGATCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 419
Bbv12I GWGCWC 3 cut(s) 29, 272, 419
BbvI GCAGC 3 cut(s) 560, 742, 764
BceAI ACGGC 1 cut(s) 422
BciVI GTATCC 1 cut(s) 869
BcoDI GTCTC 3 cut(s) 76, 513, 825
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 4 cut(s) 50, 428, 498, 660
BfmI CTRYAG 2 cut(s) 747, 777
BfuI GTATCC 1 cut(s) 869
BisI GCNGC 4 cut(s) 6, 549, 753, 756
BlsI GCNGC 4 cut(s) 7, 550, 754, 757
Bme18I GGWCC 2 cut(s) 307, 476
BmgT120I GGNCC 3 cut(s) 109, 307, 476
BmiI GGNNCC 3 cut(s) 91, 110, 478
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 5 cut(s) 309, 346, 352, 478, 853
BmuI ACTGGG 1 cut(s) 115
BplI GAGNNNNNCTC 4 cut(s) 11, 43, 641, 673
BpuEI CTTGAG 2 cut(s) 15, 403
BsaHI GRCGYC 1 cut(s) 435
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 366, 830
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 4 cut(s) 110, 574, 604, 877
Bse3DI GCAATG 1 cut(s) 205
BseLI CCNNNNNNNGG 3 cut(s) 118, 366, 830
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 227, 524
BseNI ACTGG 4 cut(s) 110, 574, 604, 877
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 3 cut(s) 560, 742, 764
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 3 cut(s) 111, 204, 424
BsiHKAI GWGCWC 3 cut(s) 29, 272, 419
BsiSI CCGG 2 cut(s) 87, 228
BslI CCNNNNNNNGG 3 cut(s) 118, 366, 830
BsmAI GTCTC 3 cut(s) 76, 513, 825
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 111, 204, 424
Bsp1286I GDGCHC 4 cut(s) 29, 272, 419, 563
Bsp143I GATC 5 cut(s) 89, 216, 238, 245, 784
BspACI CCGC 3 cut(s) 324, 474, 554
BspANI GGCC 3 cut(s) 111, 204, 424
BspCNI CTCAG 2 cut(s) 226, 523
BspLI GGNNCC 3 cut(s) 91, 110, 478
BspPI GGATC 3 cut(s) 84, 97, 233
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 4 cut(s) 110, 574, 604, 877
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 5 cut(s) 89, 216, 238, 245, 784
BssNI GRCGYC 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 43, 192, 881
BstACI GRCGYC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 3 cut(s) 213, 510, 541
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 5 cut(s) 92, 219, 241, 248, 787
BstMAI GTCTC 3 cut(s) 76, 513, 825
BstMBI GATC 5 cut(s) 89, 216, 238, 245, 784
BstMWI GCNNNNNNNGC 2 cut(s) 499, 749
BstNSI RCATGY 1 cut(s) 147
BstSFI CTRYAG 2 cut(s) 747, 777
BstSLI GKGCMC 1 cut(s) 563
BstV1I GCAGC 3 cut(s) 560, 742, 764
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BsuI GTATCC 1 cut(s) 869
BsuRI GGCC 3 cut(s) 111, 204, 424
BtsI GCAGTG 3 cut(s) 232, 563, 904
BtsIMutI CAGTG 6 cut(s) 39, 232, 563, 597, 870, 904
Cac8I GCNNGC 1 cut(s) 495
CaiI CAGNNNCTG 1 cut(s) 755
CfoI GCGC 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 109, 307, 476
CseI GACGC 1 cut(s) 443
Csp6I GTAC 1 cut(s) 538
CviAII CATG 3 cut(s) 10, 144, 841
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 3 cut(s) 213, 510, 541
DpnI GATC 5 cut(s) 91, 218, 240, 247, 786
DpnII GATC 5 cut(s) 89, 216, 238, 245, 784
DraIII CACNNNGTG 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 202
EciI GGCGGA 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 419
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 2 cut(s) 307, 476
Eco53kI GAGCTC 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 417
EcoO109I RGGNCCY 1 cut(s) 307
EcoRV GATATC 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 419
FaeI CATG 3 cut(s) 13, 147, 844
FatI CATG 3 cut(s) 9, 143, 840
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 4 cut(s) 6, 549, 753, 756
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 4 cut(s) 6, 549, 753, 756
FspBI CTAG 4 cut(s) 50, 428, 498, 660
GlaI GCGC 1 cut(s) 492
GluI GCNGC 4 cut(s) 6, 549, 753, 756
GsaI CCCAGC 1 cut(s) 369
HaeIII GGCC 3 cut(s) 111, 204, 424
HapII CCGG 2 cut(s) 87, 228
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 493
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 3 cut(s) 13, 147, 844
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 1 cut(s) 20
HpaII CCGG 2 cut(s) 87, 228
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 7 cut(s) 15, 103, 172, 538, 597, 654, 699
Hpy188I TCNGA 3 cut(s) 216, 355, 789
Hpy188III TCNNGA 2 cut(s) 466, 569
Hpy8I GTNNAC 7 cut(s) 15, 103, 172, 538, 597, 654, 699
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 2 cut(s) 119, 320
HpyCH4III ACNGT 3 cut(s) 43, 192, 881
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 499, 749
HpyF3I CTNAG 3 cut(s) 213, 510, 541
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 3 cut(s) 13, 147, 844
HspAI GCGC 1 cut(s) 491
Kzo9I GATC 5 cut(s) 89, 216, 238, 245, 784
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 3 cut(s) 560, 742, 764
LweI GCATC 5 cut(s) 309, 346, 352, 478, 853
MaeI CTAG 4 cut(s) 50, 428, 498, 660
MaeIII GTNAC 2 cut(s) 146, 230
MalI GATC 5 cut(s) 91, 218, 240, 247, 786
MboI GATC 5 cut(s) 89, 216, 238, 245, 784
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 4 cut(s) 29, 272, 419, 563
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 6 cut(s) 220, 283, 337, 394, 626, 722
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 9 cut(s) 68, 194, 222, 398, 435, 518, 522, 667, 827
Mox20I TGGCCA 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 395
MscI TGGCCA 1 cut(s) 204
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 2 cut(s) 486, 556
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 2 cut(s) 755, 782
MspI CCGG 2 cut(s) 87, 228
Mva1269I GAATGC 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 499, 749
NdeI CATATG 1 cut(s) 386
NdeII GATC 5 cut(s) 89, 216, 238, 245, 784
NlaIII CATG 3 cut(s) 13, 147, 844
NlaIV GGNNCC 3 cut(s) 91, 110, 478
NmuCI GTSAC 2 cut(s) 146, 230
NspI RCATGY 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 556
PceI AGGCCT 1 cut(s) 424
PctI GAATGC 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 395
PkrI GCNGC 4 cut(s) 7, 550, 754, 757
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 91, 110, 478
PspPI GGNCC 3 cut(s) 109, 307, 476
PspPPI RGGWCCY 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 1 cut(s) 89
PvuII CAGCTG 2 cut(s) 755, 782
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 2 cut(s) 486, 556
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 4 cut(s) 6, 549, 753, 756
Sau3AI GATC 5 cut(s) 89, 216, 238, 245, 784
Sau96I GGNCC 3 cut(s) 109, 307, 476
SchI GAGTC 1 cut(s) 29
SduI GDGCHC 4 cut(s) 29, 272, 419, 563
SfaNI GCATC 5 cut(s) 309, 346, 352, 478, 853
SfcI CTRYAG 2 cut(s) 747, 777
SinI GGWCC 2 cut(s) 307, 476
SmiMI CAYNNNNRTG 2 cut(s) 486, 556
SmlI CTYRAG 2 cut(s) 30, 418
SmoI CTYRAG 2 cut(s) 30, 418
SpeI ACTAGT 1 cut(s) 49
Sse9I AATT 6 cut(s) 220, 283, 337, 394, 626, 722
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 3 cut(s) 324, 474, 554
SspMI CTAG 4 cut(s) 50, 428, 498, 660
SstI GAGCTC 1 cut(s) 419
StuI AGGCCT 1 cut(s) 424
TaaI ACNGT 3 cut(s) 43, 192, 881
TaqI TCGA 3 cut(s) 413, 459, 465
TasI AATT 6 cut(s) 220, 283, 337, 394, 626, 722
TatI WGTACW 1 cut(s) 537
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 6 cut(s) 46, 239, 563, 604, 877, 904
TseFI GTSAC 2 cut(s) 146, 230
TseI GCWGC 4 cut(s) 5, 548, 752, 755
Tsp45I GTSAC 2 cut(s) 146, 230
TspRI CASTG 6 cut(s) 46, 239, 563, 604, 877, 904
VpaK11BI GGWCC 2 cut(s) 307, 476
XceI RCATGY 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 4 cut(s) 50, 428, 498, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.