pycom15g35750

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007530
Physical Location & Seq
Forward (+)
35816167 .. 35817072
906 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35750.1

Sequence Viewer

Length: 906 bp
ATGGCTTCCCTGTATACCAAGTCTTGCTCTAAAGTTGTCATTACCCTCCTACTGATTCTCCCATTTTTGGTTTCAAGATCGTGCGCCGGGCTCATTGTGGTTTACTGGGGCCAAAATGGAGGGGAGGGCACACTAACAGCAACATGTAACACAGGAAGGTACCGGATTGTAAACATAGCATTCCTCTCACAGTTTGGCAATGGCCAAACCCCTCAAATCAACTTAGCCGGCCACTGTGTTCCGGCGTCTAACGGCTGTCAAAAAATGAGCATCGACATCAGAAACTGCCAGAACCAAGGCATTAAGGTCCTGCTTTCGATTGGTGGCAGCAGGGGGAGTTACACGCTATCATCTGCAGATGATGCTAGAGGTGTAGCGGACTACATATGGAATAACTTCTTAGGGGGGCAATCAAACTCGAGGCCTCTAGGAGACGCCGTATTGGATGGCGTGGATTTCGATATCGAGAGAGGTGATATTCACTACGATGCACTTGCTAGGAGGCTGTCTGAGTATAGCCAGAGAGGCAGAAAAGTGTACTTAAGTGCAGCACCCCAATGTCCGTTTCCTGACCAGAAACTCAATGGTGCACTAACCACTGGCCTATTTGACTATGTTTGGGTTCAATTCTACAACAATCGACAGTGTGAATTCACCTCTAGCAATCCAGATGCCTTCAAGAACTCGTGGAACAAATGGACCTCCTCAATACGAGCTGATCGGTTCTTTGTGGGACTCCCTGCTGCGCAGGCGGCTGCTGGCACTGGCTATGTGCCTTCAAATGATCTGATAAACCAAGTTTTGCCTTTTGTTAAGAAATCTGCGAAGTATGGAGGTGTCATGTTGTATGATAAGTTCAATGATGACCAGAGTGGCTATAGTTCAAAGATTCAAGGAAGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

302

Amino Acids

32.83

Weight (kDa)

8.84

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 32 - 227 9.6e-22 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 747
Acc65I GGTACC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 14
AciI CCGC 2 cut(s) 377, 752
AcoI YGGCCR 2 cut(s) 202, 229
AcsI RAATTY 1 cut(s) 650
AcyI GRCGYC 2 cut(s) 245, 435
AfaI GTAC 2 cut(s) 161, 539
AfiI CCNNNNNNNGG 1 cut(s) 67
AflII CTTAAG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 7 cut(s) 75, 626, 679, 780, 859, 885, 893
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AluBI AGCT 1 cut(s) 716
AluI AGCT 1 cut(s) 716
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 426
Alw44I GTGCAC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 5 cut(s) 109, 202, 229, 422, 601
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 4 cut(s) 327, 548, 743, 755
ApoI RAATTY 1 cut(s) 650
Asp700I GAANNNNTTC 1 cut(s) 395
Asp718I GGTACC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 86, 748
AspS9I GGNCC 3 cut(s) 109, 307, 699
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 485, 646
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 2 cut(s) 307, 699
BaeGI GKGCMC 2 cut(s) 131, 592
BalI TGGCCA 1 cut(s) 204
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 93
BauI CACGAG 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 4 cut(s) 339, 560, 730, 742
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 268, 422
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 4 cut(s) 366, 428, 498, 660
BfmI CTRYAG 2 cut(s) 354, 877
BfrI CTTAAG 1 cut(s) 541
BglI GCCNNNNNGGC 1 cut(s) 525
BisI GCNGC 5 cut(s) 328, 549, 744, 753, 756
BlsI GCNGC 5 cut(s) 329, 550, 745, 754, 757
Bme1390I CCNGG 1 cut(s) 88
Bme18I GGWCC 2 cut(s) 307, 699
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 3 cut(s) 109, 307, 699
BmiI GGNNCC 2 cut(s) 110, 161
BmrFI CCNGG 1 cut(s) 88
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 4 cut(s) 279, 352, 478, 661
BmuI ACTGGG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 88
BsaHI GRCGYC 2 cut(s) 245, 435
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 3 cut(s) 110, 604, 769
Bse3DI GCAATG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 3 cut(s) 110, 604, 769
BseRI GAGGAG 1 cut(s) 694
BseSI GKGCMC 2 cut(s) 131, 592
BseXI GCAGC 4 cut(s) 339, 560, 730, 742
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 5 cut(s) 111, 204, 231, 424, 603
BshNI GGYRCC 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 4 cut(s) 87, 163, 228, 242
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 426
BsmBI CGTCTC 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 747
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 5 cut(s) 111, 204, 231, 424, 603
BsoBI CYCGRG 1 cut(s) 418
Bsp1286I GDGCHC 3 cut(s) 93, 131, 592
Bsp143I GATC 3 cut(s) 77, 718, 784
BspACI CCGC 2 cut(s) 377, 752
BspANI GGCC 5 cut(s) 111, 204, 231, 424, 603
BspCNI CTCAG 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 110, 161
BspMAI CTGCAG 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 159
BspTI CTTAAG 1 cut(s) 541
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 3 cut(s) 110, 604, 769
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 3 cut(s) 77, 718, 784
BssNAI GTATAC 1 cut(s) 15
BssNI GRCGYC 2 cut(s) 245, 435
BssSI CACGAG 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 295
Bst1107I GTATAC 1 cut(s) 15
Bst2BI CACGAG 1 cut(s) 685
Bst4CI ACNGT 3 cut(s) 192, 236, 645
BstACI GRCGYC 2 cut(s) 245, 435
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 3 cut(s) 229, 750, 760
BstDEI CTNAG 3 cut(s) 223, 400, 510
BstF5I GGATG 1 cut(s) 451
BstHHI GCGC 2 cut(s) 86, 748
BstKTI GATC 3 cut(s) 80, 721, 787
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 3 cut(s) 77, 718, 784
BstMWI GCNNNNNNNGC 4 cut(s) 362, 525, 749, 752
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 2 cut(s) 354, 877
BstSLI GKGCMC 2 cut(s) 131, 592
BstV1I GCAGC 4 cut(s) 339, 560, 730, 742
BstZ17I GTATAC 1 cut(s) 15
BsuRI GGCC 5 cut(s) 111, 204, 231, 424, 603
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 4 cut(s) 232, 597, 650, 762
Cac8I GCNNGC 3 cut(s) 229, 750, 760
CaiI CAGNNNCTG 1 cut(s) 285
CfoI GCGC 2 cut(s) 86, 748
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 109, 307, 699
CseI GACGC 2 cut(s) 234, 443
Csp6I GTAC 2 cut(s) 160, 538
CviAII CATG 2 cut(s) 144, 841
CviQI GTAC 2 cut(s) 160, 538
DdeI CTNAG 3 cut(s) 223, 400, 510
DpnI GATC 3 cut(s) 79, 720, 786
DpnII GATC 3 cut(s) 77, 718, 784
EaeI YGGCCR 2 cut(s) 202, 229
Eco130I CCWWGG 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 2 cut(s) 307, 699
Eco88I CYCGRG 1 cut(s) 418
EcoO109I RGGNCCY 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 650
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 426
FaeI CATG 2 cut(s) 147, 844
FaqI GGGAC 1 cut(s) 747
FatI CATG 2 cut(s) 143, 840
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 14
Fnu4HI GCNGC 5 cut(s) 328, 549, 744, 753, 756
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 5 cut(s) 328, 549, 744, 753, 756
FspBI CTAG 4 cut(s) 366, 428, 498, 660
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 2 cut(s) 85, 747
GluI GCNGC 5 cut(s) 328, 549, 744, 753, 756
HaeIII GGCC 5 cut(s) 111, 204, 231, 424, 603
HapII CCGG 4 cut(s) 87, 163, 228, 242
HgaI GACGC 2 cut(s) 234, 443
HhaI GCGC 2 cut(s) 86, 748
Hin1I GRCGYC 2 cut(s) 245, 435
Hin1II CATG 2 cut(s) 147, 844
Hin6I GCGC 2 cut(s) 84, 746
HinP1I GCGC 2 cut(s) 84, 746
HinfI GANTC 3 cut(s) 55, 735, 889
HpaII CCGG 4 cut(s) 87, 163, 228, 242
HphI GGTGA 2 cut(s) 485, 646
Hpy166II GTNNAC 5 cut(s) 15, 103, 172, 538, 590
Hpy188I TCNGA 3 cut(s) 281, 511, 789
Hpy188III TCNNGA 5 cut(s) 75, 466, 569, 668, 679
Hpy8I GTNNAC 5 cut(s) 15, 103, 172, 538, 590
HpyAV CCTTC 3 cut(s) 150, 685, 786
HpyCH4III ACNGT 3 cut(s) 192, 236, 645
HpyCH4V TGCA 4 cut(s) 356, 491, 548, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 525, 749, 752
HpyF3I CTNAG 3 cut(s) 223, 400, 510
Hsp92I GRCGYC 2 cut(s) 245, 435
Hsp92II CATG 2 cut(s) 147, 844
HspAI GCGC 2 cut(s) 84, 746
KpnI GGTACC 1 cut(s) 163
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Kzo9I GATC 3 cut(s) 77, 718, 784
Lsp1109I GCAGC 4 cut(s) 339, 560, 730, 742
LweI GCATC 4 cut(s) 279, 352, 478, 661
MaeI CTAG 4 cut(s) 366, 428, 498, 660
MaeIII GTNAC 2 cut(s) 146, 338
MalI GATC 3 cut(s) 79, 720, 786
MboI GATC 3 cut(s) 77, 718, 784
MhlI GDGCHC 3 cut(s) 93, 131, 592
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 2 cut(s) 626, 650
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 1 cut(s) 729
Mox20I TGGCCA 1 cut(s) 204
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 395
MscI TGGCCA 1 cut(s) 204
MseI TTAA 3 cut(s) 303, 542, 813
MslI CAYNNNNRTG 2 cut(s) 486, 556
Msp20I TGGCCA 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 4 cut(s) 87, 163, 228, 242
MspR9I CCNGG 1 cut(s) 88
Mva1269I GAATGC 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 362, 525, 749, 752
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 386
NdeII GATC 3 cut(s) 77, 718, 784
NgoMIV GCCGGC 1 cut(s) 227
NlaIII CATG 2 cut(s) 147, 844
NlaIV GGNNCC 2 cut(s) 110, 161
NsbI TGCGCA 1 cut(s) 747
NspI RCATGY 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 418
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 143
PctI GAATGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 2 cut(s) 55, 889
PkrI GCNGC 5 cut(s) 329, 550, 745, 754, 757
PleI GAGTC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 729
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 110, 161
PspPI GGNCC 3 cut(s) 109, 307, 699
PspPPI RGGWCCY 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 418
PsrI GAACNNNNNNTAC 2 cut(s) 839, 871
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 285
RsaI GTAC 2 cut(s) 161, 539
RsaNI GTAC 2 cut(s) 160, 538
RseI CAYNNNNRTG 2 cut(s) 486, 556
SaqAI TTAA 3 cut(s) 303, 542, 813
SatI GCNGC 5 cut(s) 328, 549, 744, 753, 756
Sau3AI GATC 3 cut(s) 77, 718, 784
Sau96I GGNCC 3 cut(s) 109, 307, 699
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 3 cut(s) 93, 131, 592
SetI ASST 8 cut(s) 161, 309, 373, 475, 659, 704, 718, 838
SfaNI GCATC 4 cut(s) 279, 352, 478, 661
SfcI CTRYAG 2 cut(s) 354, 877
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 2 cut(s) 307, 699
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 2 cut(s) 486, 556
SmlI CTYRAG 2 cut(s) 418, 541
SmoI CTYRAG 2 cut(s) 418, 541
Sse9I AATT 2 cut(s) 626, 650
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 2 cut(s) 377, 752
SspMI CTAG 4 cut(s) 366, 428, 498, 660
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 3 cut(s) 192, 236, 645
TaqI TCGA 6 cut(s) 273, 317, 419, 459, 465, 640
TasI AATT 2 cut(s) 626, 650
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 2 cut(s) 55, 889
Tru1I TTAA 3 cut(s) 303, 542, 813
Tru9I TTAA 3 cut(s) 303, 542, 813
TscAI CASTG 4 cut(s) 239, 604, 650, 769
TseI GCWGC 4 cut(s) 327, 548, 743, 755
TspGWI ACGGA 1 cut(s) 552
TspRI CASTG 4 cut(s) 239, 604, 650, 769
Vha464I CTTAAG 1 cut(s) 541
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 307, 699
XapI RAATTY 1 cut(s) 650
XceI RCATGY 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 14
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 4 cut(s) 366, 428, 498, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.