Rroxscaffold_4G00297990

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007530
Physical Location & Seq
Forward (+)
17869242 .. 17871886
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297990.1

Sequence Viewer

Length: 810 bp
ATGGCTGCCTTGTTCGCCAAGTTTTGTTCTCAAGCTGTCATTATTTTCCTTCTCATTGTCCCTTCCTTGGTTTCCAAGTCATGTGCTGCATGCATTGTGGTTTACTGGGGCCAAAATGAAGGTGAAGCTGGTCACTGTGATCCAGCATCCAATGGCTGCCAAGGCATCAGCAAGGGAGTTCGAAACTGCCAAAACGAAGGGATTAAGGTCCTGATTTCAATTGGTGGTTCCAATGGGAGCTACACACTATCAGATGCAGATGATGCCAGGCGTGTAGCAGACTACATATGGAATAACTTCTTAAGGGGGAAATCAAACTCAAGGCCTTTAGGCGATGCTGTTTTAGATGGTGTGGATTTCGACATTGAGAAGGGCGAACCTCACTATGCTGCACTGGCTAGGAAGTTATCCGAGTATAGCGCAAGTAGCCAAAAAGTGTTATTATCTGCAGCACCACAATGCCCATTTTCTGACCAGAAACTCAATGGTGCATTAACTACAGGCCTATTTGACTATGTTTGGGTTCAATTCTACAACAACCCACAATCTGCTGGCAGTGGGTATGTGCCTTCAAATGATATGATAAACCAGGTGTCATGCTCTATGATAGCCAAACGATTCTTTGTTGGGCTTCCGGCTTCTAGAGCAGCTGCTGGCAATGGTTATGTGCCTACAAATGATCTGATACACCAAGTGTTGCCTTTTGTTAAGAAATCTGCCAAGTATGGAGGTATCATGCTCTATGATAGGTTTGAAGATGGTAAGACTGGATACAGTTCTAAAGAATCTAAAGTTAGAAGCAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

269

Amino Acids

28.94

Weight (kDa)

8.34

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 40 - 181 5.2e-18 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 694
AfiI CCNNNNNNNGG 1 cut(s) 67
AflII CTTAAG 1 cut(s) 301
AgsI TTSAA 4 cut(s) 219, 527, 573, 755
AjnI CCWGG 2 cut(s) 266, 588
AluBI AGCT 4 cut(s) 35, 128, 240, 650
AluI AGCT 4 cut(s) 35, 128, 240, 650
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 3 cut(s) 109, 323, 502
ApeKI GCWGC 7 cut(s) 5, 86, 156, 389, 449, 647, 650
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 109, 208
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 208
BbvI GCAGC 6 cut(s) 73, 143, 376, 461, 637, 659
BccI CCATC 2 cut(s) 341, 752
BciT130I CCWGG 2 cut(s) 268, 590
BciVI GTATCC 1 cut(s) 764
BfaI CTAG 2 cut(s) 399, 642
BfmI CTRYAG 2 cut(s) 447, 498
BfrI CTTAAG 1 cut(s) 301
BfuI GTATCC 1 cut(s) 764
BisI GCNGC 7 cut(s) 6, 87, 157, 390, 450, 648, 651
BlsI GCNGC 7 cut(s) 7, 88, 158, 391, 451, 649, 652
Bme1390I CCNGG 2 cut(s) 268, 590
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 109, 208
BmiI GGNNCC 2 cut(s) 110, 229
BmrFI CCNGG 2 cut(s) 268, 590
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 5 cut(s) 155, 174, 244, 253, 325
BmuI ACTGGG 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 2 cut(s) 15, 304
BsaJI CCNNGG 2 cut(s) 66, 160
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 110, 399, 772
Bse3DI GCAATG 1 cut(s) 664
BseBI CCWGG 2 cut(s) 268, 590
BseDI CCNNGG 2 cut(s) 66, 160
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 664
BseNI ACTGG 3 cut(s) 110, 399, 772
BseXI GCAGC 6 cut(s) 73, 143, 376, 461, 637, 659
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 3 cut(s) 111, 325, 504
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 3 cut(s) 111, 325, 504
Bsp119I TTCGAA 1 cut(s) 181
Bsp143I GATC 2 cut(s) 139, 679
BspANI GGCC 3 cut(s) 111, 325, 504
BspLI GGNNCC 2 cut(s) 110, 229
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 664
BsrI ACTGG 3 cut(s) 110, 399, 772
BssECI CCNNGG 2 cut(s) 66, 160
BssMI GATC 2 cut(s) 139, 679
BssT1I CCWWGG 2 cut(s) 66, 160
Bst2UI CCWGG 2 cut(s) 268, 590
Bst4CI ACNGT 2 cut(s) 137, 776
BstAFI CTTAAG 1 cut(s) 301
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 3 cut(s) 91, 553, 655
BstF5I GGATG 1 cut(s) 146
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 2 cut(s) 142, 682
BstMBI GATC 2 cut(s) 139, 679
BstMWI GCNNNNNNNGC 6 cut(s) 14, 162, 263, 395, 426, 644
BstNI CCWGG 2 cut(s) 268, 590
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 2 cut(s) 266, 588
BstSFI CTRYAG 2 cut(s) 447, 498
BstV1I GCAGC 6 cut(s) 73, 143, 376, 461, 637, 659
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 3 cut(s) 111, 325, 504
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 146
BtsI GCAGTG 2 cut(s) 562, 808
BtsIMutI CAGTG 4 cut(s) 133, 392, 562, 808
Cac8I GCNNGC 3 cut(s) 91, 553, 655
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 2 cut(s) 109, 208
CsiI ACCWGGT 1 cut(s) 588
CviAII CATG 4 cut(s) 81, 90, 597, 736
DpnI GATC 2 cut(s) 141, 681
DpnII GATC 2 cut(s) 139, 679
DraIII CACNNNGTG 1 cut(s) 694
Eco130I CCWWGG 2 cut(s) 66, 160
Eco147I AGGCCT 2 cut(s) 325, 504
Eco47I GGWCC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 2 cut(s) 266, 588
EcoT14I CCWWGG 2 cut(s) 66, 160
EcoT22I ATGCAT 1 cut(s) 95
ErhI CCWWGG 2 cut(s) 66, 160
FaeI CATG 4 cut(s) 84, 93, 600, 739
FaqI GGGAC 1 cut(s) 44
FatI CATG 4 cut(s) 80, 89, 596, 735
FauNDI CATATG 1 cut(s) 287
Fnu4HI GCNGC 7 cut(s) 6, 87, 157, 390, 450, 648, 651
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 7 cut(s) 6, 87, 157, 390, 450, 648, 651
FspBI CTAG 2 cut(s) 399, 642
GlaI GCGC 1 cut(s) 421
GluI GCNGC 7 cut(s) 6, 87, 157, 390, 450, 648, 651
HaeIII GGCC 3 cut(s) 111, 325, 504
HapII CCGG 1 cut(s) 635
HhaI GCGC 1 cut(s) 422
Hin1II CATG 4 cut(s) 84, 93, 600, 739
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HinfI GANTC 2 cut(s) 618, 785
HpaII CCGG 1 cut(s) 635
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 4 cut(s) 253, 412, 472, 684
Hpy188III TCNNGA 2 cut(s) 211, 642
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 6 cut(s) 59, 72, 113, 191, 364, 579
HpyCH4III ACNGT 2 cut(s) 137, 776
HpyCH4V TGCA 6 cut(s) 89, 93, 257, 392, 449, 491
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 162, 263, 395, 426, 644
Hsp92II CATG 4 cut(s) 84, 93, 600, 739
HspAI GCGC 1 cut(s) 420
Kzo9I GATC 2 cut(s) 139, 679
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 6 cut(s) 73, 143, 376, 461, 637, 659
LweI GCATC 5 cut(s) 155, 174, 244, 253, 325
MabI ACCWGGT 1 cut(s) 588
MaeI CTAG 2 cut(s) 399, 642
MaeIII GTNAC 1 cut(s) 131
MalI GATC 2 cut(s) 141, 681
MboI GATC 2 cut(s) 139, 679
MboII GAAGA 1 cut(s) 767
MfeI CAATTG 1 cut(s) 219
MluCI AATT 2 cut(s) 219, 527
MnlI CCTC 2 cut(s) 390, 722
Mph1103I ATGCAT 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 5 cut(s) 204, 302, 494, 708, 808
MslI CAYNNNNRTG 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 650
MspCI CTTAAG 1 cut(s) 301
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 2 cut(s) 268, 590
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 2 cut(s) 268, 590
MwoI GCNNNNNNNGC 6 cut(s) 14, 162, 263, 395, 426, 644
NdeI CATATG 1 cut(s) 287
NdeII GATC 2 cut(s) 139, 679
NlaIII CATG 4 cut(s) 84, 93, 600, 739
NlaIV GGNNCC 2 cut(s) 110, 229
NmuCI GTSAC 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 95
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 181
PaeI GCATGC 1 cut(s) 93
PceI AGGCCT 2 cut(s) 325, 504
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 618, 785
PkrI GCNGC 7 cut(s) 7, 88, 158, 391, 451, 649, 652
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 266, 588
PspGI CCWGG 2 cut(s) 266, 588
PspN4I GGNNCC 2 cut(s) 110, 229
PspPI GGNCC 2 cut(s) 109, 208
PspPPI RGGWCCY 1 cut(s) 208
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 653
PvuII CAGCTG 1 cut(s) 650
RseI CAYNNNNRTG 1 cut(s) 457
SaqAI TTAA 5 cut(s) 204, 302, 494, 708, 808
SatI GCNGC 7 cut(s) 6, 87, 157, 390, 450, 648, 651
Sau3AI GATC 2 cut(s) 139, 679
Sau96I GGNCC 2 cut(s) 109, 208
ScrFI CCNGG 2 cut(s) 268, 590
SexAI ACCWGGT 1 cut(s) 588
SfaNI GCATC 5 cut(s) 155, 174, 244, 253, 325
SfcI CTRYAG 2 cut(s) 447, 498
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 457
SmlI CTYRAG 3 cut(s) 30, 301, 319
SmoI CTYRAG 3 cut(s) 30, 301, 319
SphI GCATGC 1 cut(s) 93
Sse9I AATT 2 cut(s) 219, 527
SseBI AGGCCT 2 cut(s) 325, 504
SspMI CTAG 2 cut(s) 399, 642
StuI AGGCCT 2 cut(s) 325, 504
StyD4I CCNGG 2 cut(s) 266, 588
StyI CCWWGG 2 cut(s) 66, 160
TaaI ACNGT 2 cut(s) 137, 776
TaqI TCGA 2 cut(s) 181, 360
TasI AATT 2 cut(s) 219, 527
TfiI GAWTC 2 cut(s) 618, 785
Tru1I TTAA 5 cut(s) 204, 302, 494, 708, 808
Tru9I TTAA 5 cut(s) 204, 302, 494, 708, 808
TscAI CASTG 4 cut(s) 140, 399, 562, 808
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 7 cut(s) 5, 86, 156, 389, 449, 647, 650
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 4 cut(s) 140, 399, 562, 808
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 208
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 1 cut(s) 93
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 2 cut(s) 399, 642
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.