pycom07g12560

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007530
Physical Location & Seq
Forward (+)
13886156 .. 13887218
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
ATGGCTTCCCTGTTTACCAAGTCTTGTTCTAAAGTTGTCATTACCTTCCTACTGATTCTCCCGTTTTTGGTTTCAAGGTCGTTCGCCGGGCGCATTGTGGTTTATTGGGGACAAAATGGAGGGGAGGGCACACTAACAGCAACATGTCACACAGGAAGGTACCGGATTGTAAACATAGCATTCCTCTCACAGTTCGGCAATGGCCAGACCCCTCAAATCAACTTAGCCGGGCACTGTGATCCGGCGTCTAACGGCTGTCAAAAAGTGAGCACCAGCATCAGAAACTGCCAGAACCAAGGCATTAAGGTCCTGCTTTCGATTGGCGGCAGCTTGGAGAGTTACACGCTACCATCTGCAGATGATGCTAGAGGTGTAGCGGACTACATATGGAATAACTTCTTAGGGGGGCAATCAAACTCGAGGCCTCTAGGAGACGCCGTATTGGATGGCGTGGATTTCGATATCGAGAGAGGTGAGATTCAGTACGCTGCACTTGCTAGGAGGCTTTCTGAGTATAGCCGGAGAGGTAGAAAAGTGTACTTAAGTGCAGCACCGCAATGTCCGTTTCCTGACCAGAAACTCAATGGTGCACTAACCACTGGGAAGTTGCTCATGAGTTTCCAAAAACAAAACCGTGAGGGAATGGTAAGCCCAAAGCGGACAATATCGTACTACGGTAGTGGAGCTGGCCCGGGAAGTTATCTTCCGCGGGCCCTAATAGTTTTGCAGTCTAGCAACCTATTTATTTATGGTCTATCTTTAAGAGCAAATTTTCAATGTATTGGAAACACGACTTGGTGTACCAACTGTGTTCTTTTTCAGTGTCCTTGTTTCCAAACATCTTTTGGCATGCTACATCTACTTAAAATTCTAACATATTTTTTTCTGGAGTGGTTGAAGATTTTCATAGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

306

Amino Acids

33.6

Weight (kDa)

9.21

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 31 - 191 2.2e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 159
AccII CGCG 1 cut(s) 709
AciI CCGC 6 cut(s) 324, 377, 554, 658, 707, 709
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 769, 867
AcyI GRCGYC 2 cut(s) 245, 435
AfaI GTAC 5 cut(s) 161, 485, 539, 671, 802
AfiI CCNNNNNNNGG 1 cut(s) 67
AflII CTTAAG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 3 cut(s) 75, 776, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 778, 810
AluBI AGCT 2 cut(s) 330, 686
AluI AGCT 2 cut(s) 330, 686
Alw21I GWGCWC 2 cut(s) 272, 592
Alw26I GTCTC 1 cut(s) 426
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 285
Ama87I CYCGRG 2 cut(s) 418, 691
AoxI GGCC 4 cut(s) 202, 422, 688, 711
ApaI GGGCCC 1 cut(s) 715
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 3 cut(s) 327, 488, 548
ApoI RAATTY 2 cut(s) 769, 867
Asp700I GAANNNNTTC 2 cut(s) 395, 902
Asp718I GGTACC 1 cut(s) 159
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 4 cut(s) 307, 689, 711, 712
AsuC2I CCSGG 4 cut(s) 88, 229, 692, 693
AsuHPI GGTGA 1 cut(s) 485
AvaI CYCGRG 2 cut(s) 418, 691
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 4 cut(s) 131, 234, 592, 715
BalI TGGCCA 1 cut(s) 204
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 715
Bbv12I GWGCWC 2 cut(s) 272, 592
BbvI GCAGC 3 cut(s) 339, 475, 560
BccI CCATC 2 cut(s) 358, 440
BceAI ACGGC 2 cut(s) 268, 422
BcnI CCSGG 4 cut(s) 88, 229, 692, 693
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 4 cut(s) 366, 428, 498, 732
BfmI CTRYAG 1 cut(s) 354
BfrI CTTAAG 1 cut(s) 541
BisI GCNGC 4 cut(s) 325, 328, 489, 549
BlsI GCNGC 4 cut(s) 326, 329, 490, 550
Bme1390I CCNGG 4 cut(s) 88, 229, 692, 693
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 418, 691
BmgT120I GGNCC 4 cut(s) 307, 689, 711, 712
BmiI GGNNCC 2 cut(s) 161, 713
BmrFI CCNGG 4 cut(s) 88, 229, 692, 693
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 285, 352
BmuI ACTGGG 1 cut(s) 609
BpmI CTGGAG 1 cut(s) 908
BpuMI CCSGG 4 cut(s) 88, 229, 692, 693
BsaHI GRCGYC 2 cut(s) 245, 435
BsaJI CCNNGG 3 cut(s) 295, 691, 707
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 604
Bse3DI GCAATG 2 cut(s) 205, 563
BseDI CCNNGG 3 cut(s) 295, 691, 707
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 2 cut(s) 205, 563
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 1 cut(s) 604
BseSI GKGCMC 4 cut(s) 131, 234, 592, 715
BseXI GCAGC 3 cut(s) 339, 475, 560
BsgI GTGCAG 2 cut(s) 474, 567
Bsh1236I CGCG 1 cut(s) 709
BshFI GGCC 4 cut(s) 204, 424, 690, 713
BshNI GGYRCC 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 272, 592
BsiHKCI CYCGRG 2 cut(s) 418, 691
BsiSI CCGG 6 cut(s) 87, 163, 228, 242, 520, 692
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 426
BsmBI CGTCTC 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 4 cut(s) 204, 424, 690, 713
BsoBI CYCGRG 2 cut(s) 418, 691
Bsp120I GGGCCC 1 cut(s) 711
Bsp1286I GDGCHC 5 cut(s) 131, 234, 272, 592, 715
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 6 cut(s) 324, 377, 554, 658, 707, 709
BspANI GGCC 4 cut(s) 204, 424, 690, 713
BspCNI CTCAG 1 cut(s) 502
BspFNI CGCG 1 cut(s) 709
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 161, 713
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 159
BspTI CTTAAG 1 cut(s) 541
BsrDI GCAATG 2 cut(s) 205, 563
BsrI ACTGG 1 cut(s) 604
BssECI CCNNGG 3 cut(s) 295, 691, 707
BssMI GATC 1 cut(s) 238
BssNI GRCGYC 2 cut(s) 245, 435
BssT1I CCWWGG 1 cut(s) 295
Bst4CI ACNGT 5 cut(s) 192, 236, 635, 677, 809
BstACI GRCGYC 2 cut(s) 245, 435
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 3 cut(s) 688, 711, 851
BstDEI CTNAG 3 cut(s) 223, 400, 510
BstDSI CCRYGG 1 cut(s) 707
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 709
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 362, 494
BstNSI RCATGY 2 cut(s) 147, 853
BstSCI CCNGG 4 cut(s) 86, 227, 690, 691
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 4 cut(s) 131, 234, 592, 715
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 3 cut(s) 339, 475, 560
BsuRI GGCC 4 cut(s) 204, 424, 690, 713
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 232, 597, 827
Cac8I GCNNGC 3 cut(s) 688, 711, 851
CaiI CAGNNNCTG 1 cut(s) 285
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 4 cut(s) 307, 689, 711, 712
Cfr42I CCGCGG 1 cut(s) 710
Cfr9I CCCGGG 1 cut(s) 691
CseI GACGC 2 cut(s) 234, 443
Csp6I GTAC 5 cut(s) 160, 484, 538, 670, 801
CviAII CATG 3 cut(s) 144, 613, 850
CviQI GTAC 5 cut(s) 160, 484, 538, 670, 801
DdeI CTNAG 3 cut(s) 223, 400, 510
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 715
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 307
Eco88I CYCGRG 2 cut(s) 418, 691
EcoO109I RGGNCCY 2 cut(s) 307, 712
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 426
FaeI CATG 3 cut(s) 147, 616, 853
FaqI GGGAC 1 cut(s) 123
FatI CATG 3 cut(s) 143, 612, 849
FauI CCCGC 1 cut(s) 702
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 4 cut(s) 325, 328, 489, 549
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 715
Fsp4HI GCNGC 4 cut(s) 325, 328, 489, 549
FspBI CTAG 4 cut(s) 366, 428, 498, 732
GlaI GCGC 1 cut(s) 92
GluI GCNGC 4 cut(s) 325, 328, 489, 549
GsuI CTGGAG 1 cut(s) 908
HaeIII GGCC 4 cut(s) 204, 424, 690, 713
HapII CCGG 6 cut(s) 87, 163, 228, 242, 520, 692
HgaI GACGC 2 cut(s) 234, 443
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 2 cut(s) 245, 435
Hin1II CATG 3 cut(s) 147, 616, 853
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 2 cut(s) 55, 478
HpaII CCGG 6 cut(s) 87, 163, 228, 242, 520, 692
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 5 cut(s) 15, 172, 538, 590, 801
Hpy188I TCNGA 2 cut(s) 281, 511
Hpy188III TCNNGA 4 cut(s) 466, 569, 613, 887
Hpy8I GTNNAC 5 cut(s) 15, 172, 538, 590, 801
HpyAV CCTTC 2 cut(s) 55, 150
HpyCH4III ACNGT 5 cut(s) 192, 236, 635, 677, 809
HpyCH4V TGCA 5 cut(s) 356, 491, 548, 590, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 494
HpyF3I CTNAG 3 cut(s) 223, 400, 510
Hsp92I GRCGYC 2 cut(s) 245, 435
Hsp92II CATG 3 cut(s) 147, 616, 853
HspAI GCGC 1 cut(s) 91
KpnI GGTACC 1 cut(s) 163
KspI CCGCGG 1 cut(s) 710
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 3 cut(s) 339, 475, 560
LweI GCATC 2 cut(s) 285, 352
MaeI CTAG 4 cut(s) 366, 428, 498, 732
MaeIII GTNAC 2 cut(s) 146, 338
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 2 cut(s) 695, 910
MhlI GDGCHC 5 cut(s) 131, 234, 272, 592, 715
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 2 cut(s) 769, 867
MluNI TGGCCA 1 cut(s) 204
Mox20I TGGCCA 1 cut(s) 204
MroXI GAANNNNTTC 2 cut(s) 395, 902
MscI TGGCCA 1 cut(s) 204
MseI TTAA 4 cut(s) 303, 542, 761, 864
MslI CAYNNNNRTG 1 cut(s) 556
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 709
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 6 cut(s) 87, 163, 228, 242, 520, 692
MspR9I CCNGG 4 cut(s) 88, 229, 692, 693
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 2 cut(s) 362, 494
NciI CCSGG 4 cut(s) 88, 229, 692, 693
NdeI CATATG 1 cut(s) 386
NdeII GATC 1 cut(s) 238
NlaIII CATG 3 cut(s) 147, 616, 853
NlaIV GGNNCC 2 cut(s) 161, 713
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 2 cut(s) 147, 853
PaeI GCATGC 1 cut(s) 853
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 1 cut(s) 612
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 143
PctI GAATGC 1 cut(s) 179
PdmI GAANNNNTTC 2 cut(s) 395, 902
PfeI GAWTC 2 cut(s) 55, 478
PkrI GCNGC 4 cut(s) 326, 329, 490, 550
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 161, 713
PspOMI GGGCCC 1 cut(s) 711
PspPI GGNCC 4 cut(s) 307, 689, 711, 712
PspPPI RGGWCCY 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 418
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 285
RsaI GTAC 5 cut(s) 161, 485, 539, 671, 802
RsaNI GTAC 5 cut(s) 160, 484, 538, 670, 801
RseI CAYNNNNRTG 1 cut(s) 556
SacII CCGCGG 1 cut(s) 710
SaqAI TTAA 4 cut(s) 303, 542, 761, 864
SatI GCNGC 4 cut(s) 325, 328, 489, 549
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 4 cut(s) 307, 689, 711, 712
ScrFI CCNGG 4 cut(s) 88, 229, 692, 693
SduI GDGCHC 5 cut(s) 131, 234, 272, 592, 715
SfaNI GCATC 2 cut(s) 285, 352
SfcI CTRYAG 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 418
Sfr303I CCGCGG 1 cut(s) 710
SgrBI CCGCGG 1 cut(s) 710
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 418
SmaI CCCGGG 1 cut(s) 693
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 2 cut(s) 418, 541
SmoI CTYRAG 2 cut(s) 418, 541
SphI GCATGC 1 cut(s) 853
Sse9I AATT 2 cut(s) 769, 867
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 6 cut(s) 324, 377, 554, 658, 707, 709
SspMI CTAG 4 cut(s) 366, 428, 498, 732
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 4 cut(s) 86, 227, 690, 691
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 5 cut(s) 192, 236, 635, 677, 809
TaqI TCGA 4 cut(s) 317, 419, 459, 465
TasI AATT 2 cut(s) 769, 867
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 327
TfiI GAWTC 2 cut(s) 55, 478
Tru1I TTAA 4 cut(s) 303, 542, 761, 864
Tru9I TTAA 4 cut(s) 303, 542, 761, 864
TscAI CASTG 3 cut(s) 239, 604, 827
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 327, 488, 548
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 895
TspGWI ACGGA 1 cut(s) 552
TspMI CCCGGG 1 cut(s) 691
TspRI CASTG 3 cut(s) 239, 604, 827
Vha464I CTTAAG 1 cut(s) 541
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 769, 867
XceI RCATGY 2 cut(s) 147, 853
XcmI CCANNNNNNNNNTGG 2 cut(s) 581, 842
XhoI CTCGAG 1 cut(s) 418
XmaI CCCGGG 1 cut(s) 691
XmnI GAANNNNTTC 2 cut(s) 395, 902
XspI CTAG 4 cut(s) 366, 428, 498, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.