pycom07g21390

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003923
Physical Location & Seq
Forward (+)
23120144 .. 23124400
4257 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1395 bp
ATGGAGGCATCTTCCGGAAATAGTAGTCCCGCCAGTGGGGGAGCCACTGAGGTTGGCAGCAATACGTCGTCGTTCGGGTCTATGCAGTTCCCCGCCCTCCGGTTCTTCCAGTCGCCGCTCTCATTCGTGTTGGATTACTCCGGAATACTCTCTCCCAGCTCTTCCCGCCGCGATTCCGACGCTCCGGTGGTCAACGCTGTAGTCGTTGCCGATTCTCAGCCCCACTCCCCTACGGCCTGCACCTCCGGGTCGAGCAACACCGGGGAGGTTTCGATTCGGATTATCGGCGCCGGCGAGCAAGAGGACCGCGGGAACGGGGGTGCGACGGGGCCGGTTCAAGGGGAGGAGAATGATGGGTCGCCGGGTAGGGTTCAATCGTTGGATGATCTGGCGGGTACAGTCAATGGGGTTGGGTCGGACGAGAGAGTCCCCTTGGTGGCTTCGTCGTCTCCCACACACGCTGGGGATACTGGGAATGGCGCGGAGGATAGTTCTAGGGATTCTGCTTACCAGAGATATGATATTCAGCAGATAGCTAGGTGGATTGAGCAGATTCTTCCGTTTTCGTTGCTCTTACTCGTTGTGTTCATTCGTCAGCATTTGCAAGGTTTCTTTGTCACTATATGGGTATCAGCTGTCATGTATAGGTCAAATGATGTTGTTAAGAAGCAGACTTCTCTAAAGGGGGATAGGAAAATCTCTGTTCTTGTAAGTATTGCTGGTGCTTTTATGCTTCATGTAGTTGTTGTTTACTGGTGGTATCGAAGTGATGATCTTCTATACCCATTGGCAATGCTTCCTCCAAAAACCATACCACCTTTCTGGCATGCCATATTTATCATTTTGGTAAATGATACCATGGTGAGACAGGCAGCGATGGCTTTCAAGTGTTTGTTGCTTATATACTACAAGAATGGCAGAGGCCATAACTATCGTCGGCAGGGTCAAATGTTAACTGTCATTGAGTATACACTGCTGCTGTACCGTGCCTTGTTACCAGCACCCGTTTGGTACAGATTCTTCCTGAACAAAGACAATGGGAGCCTCTTCTCCTCACTTACAACGGGATTATATTTAACTTTCAAGCTAACATCTGTTGTTGAGAAGGTCCAATCCTTTTTGGCTTCCATAAGGGCATTGTCACGGAAGGATATCCATTATGGGTCTCATGCAACATTGGAACAGGTAAATGCAGCAGGAGACCTTTGTGCCATTTGCCAGGAGAAGATGCATGCTCCAATTCTGTTGCGCTGTAAACATATTTTCTGCGAGGATTGTGTCTCTGAATGGTTTGAGAGAGAAAGAACTTGCCCTCTATGCAGGGCACTAGTAAAACCTGCGGATTTGCGAACATATGGAGACGGATCAACGAGTTTAATTTTCCAGTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

465

Amino Acids

51.02

Weight (kDa)

6.79

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 400 - 441 5.9e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 401 - 444 6.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 402 - 441 1.9e-10 Ring finger domain
zf-C3HC4 PF00097 403 - 440 3.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 403 - 440 6.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 403 - 438 7.8e-08 RING-type zinc-finger
zf-RING_5 PF14634 403 - 441 2.7e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 1345
AccB1I GGYRCC 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 968
AccII CGCG 3 cut(s) 171, 309, 482
AccIII TCCGGA 2 cut(s) 14, 140
AclWI GGATC 1 cut(s) 1372
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 3 cut(s) 397, 983, 1013
AfiI CCNNNNNNNGG 5 cut(s) 35, 36, 99, 338, 436
AgsI TTSAA 4 cut(s) 338, 374, 886, 1084
AhlI ACTAGT 1 cut(s) 1327
AjnI CCWGG 1 cut(s) 1218
AluBI AGCT 4 cut(s) 159, 536, 635, 1087
AluI AGCT 4 cut(s) 159, 536, 635, 1087
Alw26I GTCTC 6 cut(s) 453, 859, 1170, 1194, 1285, 1353
AlwI GGATC 1 cut(s) 1372
Aor13HI TCCGGA 2 cut(s) 14, 140
AoxI GGCC 3 cut(s) 234, 329, 922
ApeKI GCWGC 4 cut(s) 57, 872, 976, 1193
AspLEI GCGC 3 cut(s) 290, 482, 1251
AspS9I GGNCC 3 cut(s) 304, 329, 1108
AsuC2I CCSGG 3 cut(s) 247, 262, 363
AsuHPI GGTGA 1 cut(s) 874
AvaII GGWCC 2 cut(s) 304, 1108
BaeGI GKGCMC 1 cut(s) 1327
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BanI GGYRCC 1 cut(s) 287
BarI GAAGNNNNNNTAC 2 cut(s) 1004, 1036
BbvI GCAGC 4 cut(s) 69, 884, 963, 1205
BccI CCATC 2 cut(s) 347, 871
BceAI ACGGC 1 cut(s) 249
BciT130I CCWGG 1 cut(s) 1220
BciVI GTATCC 1 cut(s) 460
BcnI CCSGG 3 cut(s) 247, 262, 363
BcoDI GTCTC 6 cut(s) 453, 859, 1170, 1194, 1285, 1353
BcuI ACTAGT 1 cut(s) 1327
BfaI CTAG 3 cut(s) 495, 537, 1328
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 1345
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 6 cut(s) 58, 116, 169, 873, 977, 1194
BlsI GCNGC 6 cut(s) 59, 117, 170, 874, 978, 1195
Bme1390I CCNGG 4 cut(s) 247, 262, 363, 1220
Bme18I GGWCC 2 cut(s) 304, 1108
BmgT120I GGNCC 3 cut(s) 304, 329, 1108
BmiI GGNNCC 4 cut(s) 43, 289, 330, 1043
BmrFI CCNGG 4 cut(s) 247, 262, 363, 1220
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 2 cut(s) 17, 1218
BmuI ACTGGG 1 cut(s) 480
BpuMI CCSGG 3 cut(s) 247, 262, 363
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 2 cut(s) 1170, 1194
BsaJI CCNNGG 4 cut(s) 261, 307, 432, 858
BsaWI WCCGGW 4 cut(s) 14, 99, 140, 184
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 36, 99, 338, 436
Bse118I RCCGGY 2 cut(s) 290, 331
Bse1I ACTGG 5 cut(s) 33, 109, 475, 758, 1384
Bse3DI GCAATG 1 cut(s) 798
BseAI TCCGGA 2 cut(s) 14, 140
BseBI CCWGG 1 cut(s) 1220
BseDI CCNNGG 4 cut(s) 261, 307, 432, 858
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 5 cut(s) 35, 36, 99, 338, 436
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 2 cut(s) 39, 230
BseNI ACTGG 5 cut(s) 33, 109, 475, 758, 1384
BseRI GAGGAG 2 cut(s) 359, 1042
BseSI GKGCMC 1 cut(s) 1327
BseXI GCAGC 4 cut(s) 69, 884, 963, 1205
BseYI CCCAGC 2 cut(s) 155, 461
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 3 cut(s) 171, 309, 482
BshFI GGCC 3 cut(s) 236, 331, 924
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 9 cut(s) 15, 100, 141, 185, 246, 261, 291, 332, 362
BslFI GGGAC 2 cut(s) 12, 413
BslI CCNNNNNNNGG 5 cut(s) 35, 36, 99, 338, 436
BsmAI GTCTC 6 cut(s) 453, 859, 1170, 1194, 1285, 1353
BsmBI CGTCTC 2 cut(s) 453, 1353
BsmFI GGGAC 2 cut(s) 12, 413
BsnI GGCC 3 cut(s) 236, 331, 924
Bso31I GGTCTC 2 cut(s) 1170, 1194
Bsp1286I GDGCHC 1 cut(s) 1327
Bsp13I TCCGGA 2 cut(s) 14, 140
Bsp143I GATC 3 cut(s) 385, 772, 1364
Bsp19I CCATGG 1 cut(s) 858
BspANI GGCC 3 cut(s) 236, 331, 924
BspCNI CTCAG 2 cut(s) 40, 229
BspEI TCCGGA 2 cut(s) 14, 140
BspFNI CGCG 3 cut(s) 171, 309, 482
BspLI GGNNCC 4 cut(s) 43, 289, 330, 1043
BspMI ACCTGC 1 cut(s) 1345
BspPI GGATC 1 cut(s) 1372
BspQI GCTCTTC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 1170, 1194
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 798
BsrFI RCCGGY 2 cut(s) 290, 331
BsrI ACTGG 5 cut(s) 33, 109, 475, 758, 1384
BssAI RCCGGY 2 cut(s) 290, 331
BssECI CCNNGG 4 cut(s) 261, 307, 432, 858
BssMI GATC 3 cut(s) 385, 772, 1364
BssNAI GTATAC 1 cut(s) 969
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 2 cut(s) 432, 858
Bst1107I GTATAC 1 cut(s) 969
Bst2UI CCWGG 1 cut(s) 1220
Bst4CI ACNGT 3 cut(s) 400, 958, 986
Bst6I CTCTTC 2 cut(s) 166, 1052
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 5 cut(s) 238, 292, 296, 828, 1233
BstDEI CTNAG 2 cut(s) 48, 216
BstDSI CCRYGG 2 cut(s) 307, 858
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 3 cut(s) 171, 309, 482
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 3 cut(s) 290, 482, 1251
BstKTI GATC 3 cut(s) 388, 775, 1367
BstMAI GTCTC 6 cut(s) 453, 859, 1170, 1194, 1285, 1353
BstMBI GATC 3 cut(s) 385, 772, 1364
BstMWI GCNNNNNNNGC 3 cut(s) 165, 878, 1317
BstNI CCWGG 1 cut(s) 1220
BstNSI RCATGY 2 cut(s) 830, 1235
BstSCI CCNGG 4 cut(s) 245, 260, 361, 1218
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 1327
BstUI CGCG 3 cut(s) 171, 309, 482
BstV1I GCAGC 4 cut(s) 69, 884, 963, 1205
BstXI CCANNNNNNTGG 1 cut(s) 822
BstZ17I GTATAC 1 cut(s) 969
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 3 cut(s) 236, 331, 924
BtgI CCRYGG 2 cut(s) 307, 858
BtgZI GCGATG 1 cut(s) 890
BtsCI GGATG 1 cut(s) 388
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 3 cut(s) 40, 45, 971
BveI ACCTGC 1 cut(s) 1345
Cac8I GCNNGC 5 cut(s) 238, 292, 296, 828, 1233
CfoI GCGC 3 cut(s) 290, 482, 1251
Cfr10I RCCGGY 2 cut(s) 290, 331
Cfr13I GGNCC 3 cut(s) 304, 329, 1108
Cfr42I CCGCGG 1 cut(s) 310
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 396, 982, 1012
CspCI CAANNNNNGTGG 2 cut(s) 34, 69
CviAII CATG 6 cut(s) 640, 737, 827, 859, 1169, 1232
CviQI GTAC 3 cut(s) 396, 982, 1012
DdeI CTNAG 2 cut(s) 48, 216
DinI GGCGCC 1 cut(s) 289
DpnI GATC 3 cut(s) 387, 774, 1366
DpnII GATC 3 cut(s) 385, 772, 1364
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
Eam1104I CTCTTC 2 cut(s) 166, 1052
EarI CTCTTC 2 cut(s) 166, 1052
Eco130I CCWWGG 2 cut(s) 432, 858
Eco31I GGTCTC 2 cut(s) 1170, 1194
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 2 cut(s) 304, 1108
EcoRII CCWGG 1 cut(s) 1218
EcoRV GATATC 1 cut(s) 1153
EcoT14I CCWWGG 2 cut(s) 432, 858
EcoT22I ATGCAT 1 cut(s) 1233
EgeI GGCGCC 1 cut(s) 289
EheI GGCGCC 1 cut(s) 289
ErhI CCWWGG 2 cut(s) 432, 858
Esp3I CGTCTC 2 cut(s) 453, 1353
FaeI CATG 6 cut(s) 643, 740, 830, 862, 1172, 1235
FaqI GGGAC 2 cut(s) 12, 413
FatI CATG 6 cut(s) 639, 736, 826, 858, 1168, 1231
FauI CCCGC 5 cut(s) 37, 100, 173, 302, 385
FauNDI CATATG 1 cut(s) 1354
FblI GTMKAC 1 cut(s) 968
Fnu4HI GCNGC 6 cut(s) 58, 116, 169, 873, 977, 1194
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 6 cut(s) 58, 116, 169, 873, 977, 1194
FspBI CTAG 3 cut(s) 495, 537, 1328
GlaI GCGC 3 cut(s) 289, 481, 1250
GluI GCNGC 6 cut(s) 58, 116, 169, 873, 977, 1194
GsaI CCCAGC 2 cut(s) 159, 465
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 3 cut(s) 236, 331, 924
HapII CCGG 9 cut(s) 15, 100, 141, 185, 246, 261, 291, 332, 362
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 290, 482, 1251
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 6 cut(s) 643, 740, 830, 862, 1172, 1235
Hin6I GCGC 3 cut(s) 288, 480, 1249
HinP1I GCGC 3 cut(s) 288, 480, 1249
HincII GTYRAC 2 cut(s) 193, 954
HindII GTYRAC 2 cut(s) 193, 954
HinfI GANTC 7 cut(s) 173, 212, 274, 426, 500, 553, 1017
HpaI GTTAAC 1 cut(s) 954
HpaII CCGG 9 cut(s) 15, 100, 141, 185, 246, 261, 291, 332, 362
HphI GGTGA 1 cut(s) 874
Hpy166II GTNNAC 5 cut(s) 193, 751, 954, 969, 1256
Hpy188I TCNGA 5 cut(s) 178, 279, 418, 1285, 1394
Hpy188III TCNNGA 3 cut(s) 15, 141, 1024
Hpy8I GTNNAC 5 cut(s) 193, 751, 954, 969, 1256
Hpy99I CGWCG 6 cut(s) 70, 73, 182, 328, 448, 939
HpyAV CCTTC 2 cut(s) 1099, 1141
HpyCH4III ACNGT 3 cut(s) 400, 958, 986
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 7 cut(s) 85, 240, 604, 1172, 1193, 1231, 1320
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 878, 1317
HpyF3I CTNAG 2 cut(s) 48, 216
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 6 cut(s) 643, 740, 830, 862, 1172, 1235
HspAI GCGC 3 cut(s) 288, 480, 1249
KasI GGCGCC 1 cut(s) 287
Kpn2I TCCGGA 2 cut(s) 14, 140
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 954
KspI CCGCGG 1 cut(s) 310
Kzo9I GATC 3 cut(s) 385, 772, 1364
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 4 cut(s) 41, 187, 1041, 1240
Lsp1109I GCAGC 4 cut(s) 69, 884, 963, 1205
LweI GCATC 2 cut(s) 17, 1218
MaeI CTAG 3 cut(s) 495, 537, 1328
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 3 cut(s) 616, 993, 1140
MalI GATC 3 cut(s) 387, 774, 1366
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 385, 772, 1364
MboII GAAGA 8 cut(s) 3, 97, 153, 548, 767, 1012, 1039, 1237
MhlI GDGCHC 1 cut(s) 1327
MluCI AATT 2 cut(s) 1239, 1377
Mly113I GGCGCC 1 cut(s) 288
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 4 cut(s) 111, 201, 360, 396
Mph1103I ATGCAT 1 cut(s) 1233
MreI CGCCGGCG 1 cut(s) 290
MroI TCCGGA 2 cut(s) 14, 140
MroNI GCCGGC 1 cut(s) 290
MseI TTAA 4 cut(s) 663, 953, 1076, 1376
MspA1I CMGCKG 2 cut(s) 309, 635
MspI CCGG 9 cut(s) 15, 100, 141, 185, 246, 261, 291, 332, 362
MspR9I CCNGG 4 cut(s) 247, 262, 363, 1220
MvaI CCWGG 1 cut(s) 1220
MvnI CGCG 3 cut(s) 171, 309, 482
MwoI GCNNNNNNNGC 3 cut(s) 165, 878, 1317
NaeI GCCGGC 1 cut(s) 292
NarI GGCGCC 1 cut(s) 288
NciI CCSGG 3 cut(s) 247, 262, 363
NcoI CCATGG 1 cut(s) 858
NdeI CATATG 1 cut(s) 1354
NdeII GATC 3 cut(s) 385, 772, 1364
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 6 cut(s) 643, 740, 830, 862, 1172, 1235
NlaIV GGNNCC 4 cut(s) 43, 289, 330, 1043
NmuCI GTSAC 2 cut(s) 616, 1140
NsiI ATGCAT 1 cut(s) 1233
NspI RCATGY 2 cut(s) 830, 1235
PaeI GCATGC 2 cut(s) 830, 1235
PciSI GCTCTTC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 291
PdiI GCCGGC 1 cut(s) 292
PfeI GAWTC 6 cut(s) 173, 212, 274, 500, 553, 1017
PkrI GCNGC 6 cut(s) 59, 117, 170, 874, 978, 1195
PleI GAGTC 1 cut(s) 434
PluTI GGCGCC 1 cut(s) 291
PpsI GAGTC 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 1218
PspFI CCCAGC 2 cut(s) 155, 461
PspGI CCWGG 1 cut(s) 1218
PspN4I GGNNCC 4 cut(s) 43, 289, 330, 1043
PspPI GGNCC 3 cut(s) 304, 329, 1108
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 3 cut(s) 397, 983, 1013
RsaNI GTAC 3 cut(s) 396, 982, 1012
SacII CCGCGG 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 4 cut(s) 663, 953, 1076, 1376
SatI GCNGC 6 cut(s) 58, 116, 169, 873, 977, 1194
Sau3AI GATC 3 cut(s) 385, 772, 1364
Sau96I GGNCC 3 cut(s) 304, 329, 1108
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 4 cut(s) 247, 262, 363, 1220
SduI GDGCHC 1 cut(s) 1327
SfaNI GCATC 2 cut(s) 17, 1218
SfcI CTRYAG 1 cut(s) 198
SfoI GGCGCC 1 cut(s) 289
Sfr303I CCGCGG 1 cut(s) 310
SgrAI CRCCGGYG 1 cut(s) 290
SgrBI CCGCGG 1 cut(s) 310
SinI GGWCC 2 cut(s) 304, 1108
SpeI ACTAGT 1 cut(s) 1327
SphI GCATGC 2 cut(s) 830, 1235
Sse9I AATT 2 cut(s) 1239, 1377
SspDI GGCGCC 1 cut(s) 287
SspMI CTAG 3 cut(s) 495, 537, 1328
StyD4I CCNGG 4 cut(s) 245, 260, 361, 1218
StyI CCWWGG 2 cut(s) 432, 858
TaaI ACNGT 3 cut(s) 400, 958, 986
TaiI ACGT 1 cut(s) 68
TaqI TCGA 3 cut(s) 251, 272, 763
TasI AATT 2 cut(s) 1239, 1377
TauI GCSGC 2 cut(s) 118, 171
TfiI GAWTC 6 cut(s) 173, 212, 274, 500, 553, 1017
Tru1I TTAA 4 cut(s) 663, 953, 1076, 1376
Tru9I TTAA 4 cut(s) 663, 953, 1076, 1376
TscAI CASTG 3 cut(s) 40, 52, 978
TseFI GTSAC 2 cut(s) 616, 1140
TseI GCWGC 4 cut(s) 57, 872, 976, 1193
Tsp45I GTSAC 2 cut(s) 616, 1140
TspDTI ATGAA 2 cut(s) 577, 725
TspGWI ACGGA 3 cut(s) 549, 1159, 1377
TspRI CASTG 3 cut(s) 40, 52, 978
VpaK11BI GGWCC 2 cut(s) 304, 1108
XceI RCATGY 2 cut(s) 830, 1235
XcmI CCANNNNNNNNNTGG 1 cut(s) 1005
XmiI GTMKAC 1 cut(s) 968
XspI CTAG 3 cut(s) 495, 537, 1328
Zsp2I ATGCAT 1 cut(s) 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.