pycom07g24600

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007411
Physical Location & Seq
Reverse (-)
25242417 .. 25245688
3272 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGGAGGAGGCCCACGTGTCATCTATACACGAAGTTTGTGATCCCAATCCGACGGTCCAATTGACTTGCCATAATCAGGTGGAAAAGTTAAGGAGGGAGGAGGAGGAGAGAGAGAGAGAGAGAGAGAAAGAGATGGCGCTAAAGGTACCGGCGCTTCCGTTCACGTTTGTGGTACACACGCTCGGAATCGTGGCTTTGGTTTTGGTGTTGGTTTGGAACATTCATTTCAGAGGCGGCTTGGCATGGGAAGCTACCAACAAGAACCTCATCTTCAACCTCCATCCTGTTCTCATGTTACTTGGCCTAATTTTCTTGGGAGGAGAAGCCATTGTTAGTTACAAAGCACTGCCTTTCAGGAAAGAGGTGAAGAAAGTGATACACTTGGTTCTTCATGCCCTCGCGTTAGGTCTTGGGATCATTGGTATTTATCTTGCATTCAAGAACCATAACGAGAGTGGAATTGCCAATCTGTACAGTTTGCACTCTTGGATTGGAATCGGGGTCATTTGTCTCTATGGAATTCAGTGGATATATGGGTTCGTGACCTTCTTCTACCCCGGAGGAAGTCCTGCACTGAGAAGTGAGTCTATCCCATGGCATGTGGCGTTTGGAATCACCGTGTACTTACTAGCTGTTGGCAATGCTTCATTAGGGTTCTTAGAGAAGCTCACGTTCCTCGAGAACTCGGGAATCGATAAATTTGGGACGGAGGCCTTTCTCGTCAACTTCACCGCAATCATCACTGTACTGTTCGGAGTCTTCGTTATACTCTCAGTACTTTCTGAAGCTCCTCCTTCTGTAGAAGATGACAACAGCTATTCTGCTATTTGA

Protein Analysis

277

Amino Acids

30.66

Weight (kDa)

5.87

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 93 - 225 1.8e-47 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 1 cut(s) 401
AciI CCGC 2 cut(s) 234, 732
AclWI GGATC 2 cut(s) 35, 422
AcsI RAATTY 2 cut(s) 519, 698
AcuI CTGAAG 1 cut(s) 804
AcvI CACGTG 1 cut(s) 16
AfaI GTAC 6 cut(s) 147, 174, 473, 623, 747, 777
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 2 cut(s) 274, 439
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 5 cut(s) 251, 632, 667, 788, 816
AluI AGCT 5 cut(s) 251, 632, 667, 788, 816
Alw26I GTCTC 1 cut(s) 515
AlwI GGATC 2 cut(s) 35, 422
Ama87I CYCGRG 2 cut(s) 677, 685
AoxI GGCC 3 cut(s) 9, 301, 711
ApoI RAATTY 2 cut(s) 519, 698
Asp718I GGTACC 1 cut(s) 145
AspLEI GCGC 2 cut(s) 139, 154
AspS9I GGNCC 2 cut(s) 10, 55
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 3 cut(s) 376, 607, 721
AvaI CYCGRG 2 cut(s) 677, 685
AvaII GGWCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 138, 170
BbrPI CACGTG 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 751
BccI CCATC 2 cut(s) 127, 288
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 515
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 2 cut(s) 140, 155
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
BmcAI AGTACT 1 cut(s) 777
Bme1390I CCNGG 1 cut(s) 558
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 2 cut(s) 677, 685
BmgT120I GGNCC 2 cut(s) 10, 55
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 558
BpiI GAAGAC 1 cut(s) 751
BpuMI CCSGG 1 cut(s) 558
Bsa29I ATCGAT 1 cut(s) 693
BsaAI YACGTR 1 cut(s) 16
BsaBI GATNNNNATC 2 cut(s) 45, 494
BsaJI CCNNGG 2 cut(s) 556, 593
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 646
Bse8I GATNNNNATC 2 cut(s) 45, 494
BseCI ATCGAT 1 cut(s) 693
BseDI CCNNGG 2 cut(s) 556, 593
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 2 cut(s) 45, 494
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 2 cut(s) 566, 786
BseRI GAGGAG 6 cut(s) 20, 113, 116, 119, 333, 780
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 3 cut(s) 11, 303, 713
BshNI GGYRCC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 693
BsiHKCI CYCGRG 2 cut(s) 677, 685
BsiSI CCGG 2 cut(s) 149, 558
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 515
BsmFI GGGAC 1 cut(s) 718
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 3 cut(s) 11, 303, 713
BsoBI CYCGRG 2 cut(s) 677, 685
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 2 cut(s) 40, 414
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 2 cut(s) 234, 732
BspANI GGCC 3 cut(s) 11, 303, 713
BspCNI CTCAG 2 cut(s) 567, 785
BspDI ATCGAT 1 cut(s) 693
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 1 cut(s) 147
BspPI GGATC 2 cut(s) 35, 422
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 646
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 556, 593
BssMI GATC 2 cut(s) 40, 414
BssT1I CCWWGG 1 cut(s) 593
Bst4CI ACNGT 5 cut(s) 55, 476, 619, 745, 750
BstAUI TGTACA 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 575, 658, 772
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 401
BstH2I RGCGCY 2 cut(s) 140, 155
BstHHI GCGC 2 cut(s) 139, 154
BstKTI GATC 2 cut(s) 43, 417
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 2 cut(s) 40, 414
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 556
BstSFI CTRYAG 1 cut(s) 798
BstUI CGCG 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 751
Bsu15I ATCGAT 1 cut(s) 693
BsuRI GGCC 3 cut(s) 11, 303, 713
BsuTUI ATCGAT 1 cut(s) 693
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 4 cut(s) 344, 530, 572, 741
CfoI GCGC 2 cut(s) 139, 154
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 10, 55
ClaI ATCGAT 1 cut(s) 693
Csp6I GTAC 6 cut(s) 146, 173, 472, 622, 746, 776
CviAII CATG 5 cut(s) 243, 292, 392, 594, 599
CviQI GTAC 6 cut(s) 146, 173, 472, 622, 746, 776
DdeI CTNAG 3 cut(s) 575, 658, 772
DpnI GATC 2 cut(s) 42, 416
DpnII GATC 2 cut(s) 40, 414
Eco130I CCWWGG 1 cut(s) 593
Eco147I AGGCCT 1 cut(s) 713
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 804
Eco72I CACGTG 1 cut(s) 16
Eco88I CYCGRG 2 cut(s) 677, 685
EcoRI GAATTC 1 cut(s) 519
EcoT14I CCWWGG 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 593
FaeI CATG 5 cut(s) 246, 295, 395, 597, 602
FaqI GGGAC 1 cut(s) 718
FatI CATG 5 cut(s) 242, 291, 391, 593, 598
Fnu4HI GCNGC 1 cut(s) 235
FokI GGATG 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 1 cut(s) 629
GlaI GCGC 2 cut(s) 138, 153
GluI GCNGC 1 cut(s) 235
HaeII RGCGCY 2 cut(s) 140, 155
HaeIII GGCC 3 cut(s) 11, 303, 713
HapII CCGG 2 cut(s) 149, 558
HhaI GCGC 2 cut(s) 139, 154
Hin1II CATG 5 cut(s) 246, 295, 395, 597, 602
Hin6I GCGC 2 cut(s) 137, 152
HinP1I GCGC 2 cut(s) 137, 152
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HinfI GANTC 6 cut(s) 186, 495, 584, 612, 690, 756
HpaII CCGG 2 cut(s) 149, 558
HphI GGTGA 3 cut(s) 376, 607, 721
Hpy166II GTNNAC 4 cut(s) 162, 175, 622, 724
Hpy188I TCNGA 5 cut(s) 51, 185, 230, 755, 784
Hpy188III TCNNGA 5 cut(s) 355, 439, 541, 679, 687
Hpy8I GTNNAC 4 cut(s) 162, 175, 622, 724
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 556, 804
HpyCH4III ACNGT 5 cut(s) 55, 476, 619, 745, 750
HpyCH4IV ACGT 3 cut(s) 15, 164, 671
HpyCH4V TGCA 3 cut(s) 434, 481, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 575, 658, 772
HpySE526I ACGT 3 cut(s) 15, 164, 671
Hsp92II CATG 5 cut(s) 246, 295, 395, 597, 602
HspAI GCGC 2 cut(s) 137, 152
KpnI GGTACC 1 cut(s) 149
Kzo9I GATC 2 cut(s) 40, 414
LmnI GCTCC 1 cut(s) 793
LpnPI CCDG 6 cut(s) 62, 162, 297, 340, 571, 582
MaeI CTAG 1 cut(s) 629
MaeII ACGT 3 cut(s) 15, 164, 671
MaeIII GTNAC 3 cut(s) 294, 335, 541
MalI GATC 2 cut(s) 42, 416
MboI GATC 2 cut(s) 40, 414
MboII GAAGA 6 cut(s) 262, 379, 380, 541, 751, 815
MfeI CAATTG 1 cut(s) 59
MluCI AATT 5 cut(s) 59, 306, 459, 519, 698
MlyI GAGTC 2 cut(s) 593, 765
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 2 cut(s) 149, 558
MspR9I CCNGG 1 cut(s) 558
MunI CAATTG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 434
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 558
NcoI CCATGG 1 cut(s) 593
NdeII GATC 2 cut(s) 40, 414
NlaIII CATG 5 cut(s) 246, 295, 395, 597, 602
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 1 cut(s) 602
OliI CACNNNNGTG 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 677
PceI AGGCCT 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 4 cut(s) 186, 495, 612, 690
PkrI GCNGC 1 cut(s) 236
PleI GAGTC 2 cut(s) 592, 764
PmaCI CACGTG 1 cut(s) 16
PmlI CACGTG 1 cut(s) 16
PpsI GAGTC 2 cut(s) 592, 764
Ppu21I YACGTR 1 cut(s) 16
PspCI CACGTG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 2 cut(s) 10, 55
RsaI GTAC 6 cut(s) 147, 174, 473, 623, 747, 777
RsaNI GTAC 6 cut(s) 146, 173, 472, 622, 746, 776
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 2 cut(s) 40, 414
Sau96I GGNCC 2 cut(s) 10, 55
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 2 cut(s) 593, 765
ScrFI CCNGG 1 cut(s) 558
SfcI CTRYAG 1 cut(s) 798
Sfr274I CTCGAG 1 cut(s) 677
SinI GGWCC 1 cut(s) 55
SlaI CTCGAG 1 cut(s) 677
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 5 cut(s) 59, 306, 459, 519, 698
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 2 cut(s) 234, 732
SspMI CTAG 1 cut(s) 629
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 1 cut(s) 556
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 5 cut(s) 55, 476, 619, 745, 750
TaiI ACGT 3 cut(s) 18, 167, 674
TaqI TCGA 2 cut(s) 678, 693
TasI AATT 5 cut(s) 59, 306, 459, 519, 698
TatI WGTACW 4 cut(s) 471, 621, 745, 775
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 186, 495, 612, 690
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 4 cut(s) 351, 530, 579, 748
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 3 cut(s) 212, 380, 636
TspGWI ACGGA 2 cut(s) 147, 722
TspRI CASTG 4 cut(s) 351, 530, 579, 748
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 2 cut(s) 519, 698
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 677
XspI CTAG 1 cut(s) 629
ZrmI AGTACT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.