pycom07g25380

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002711
Physical Location & Seq
Forward (+)
25709353 .. 25712017
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1419 bp
ATGGAGACCTCCTCAAAATTCCATGTTGCTTCTGCGATTATTGTTGCGCTGGGATTATTCTTCCTAACAGCGGCAGAATGTCGAAAACCGGTTTCGGGCACGATCAATTACTCGGCCATAAACTGTAGAAAACATAGTGCTCTTTTGACCGACTTTGGAGCCGTTGGAGACGGAAAAACTTCCAACACAAAGGCCTTCAAGGCTGCCGTTGGTCATCTAAGTCAGCTTGCATCCGACGGCGGAGCGCAGCTTATTGTGCCGCCGGGGAGGTGGCTCACCGGAAGCTTCAACCTCACTAGCCACTTTACCCTCTTCCTCCATAAGGATGCCGTGATTCTTGCAACTCAGGATGAGTCAGAGTGGCCTCATCTTCCAGTACTGCCTTCATATGGGAGAGGCAGAGATGCCCCCGGTGGAAGGTTCAGCAGTCTCGTCTTCGGAACAAACCTCACCGACGTTGTAATTACCGGTAACAACGGCACAATCGACGGCCAAGGTGCTTCTTGGTGGGCGAAGTTCAAACAAGGTCACTTGACCCTCACCCGACCATACATGATCGAGATCATGTACTCTAATCAGATTCAAATTTCTAATCTCACTTTGGTCAATTCCCCTTCGTGGTTTGTCCATCCCATATACAGCAGCAATATAACAATCCAAGGGCTCACAATCCTTGCACCAGTTGATTCTCCCAACACCGATGGGATTGACCCAGATTCTTGTTCACAAACAAGAATCGAAGACTGCTACATTGTCTCTGGAGATGACTGCATCGCAGTAAAGAGTGGATGGGATGAATACGGAATCAAAGTCGGAATCCCAACGGAGCACCTTGTGATAAGAAGGCTCACTTGCATTTCACCTGACAGTGCCACCATTGCCCTAGGTAGTGAAATGTCGGGTGGAATCCGCGACGTTAGAGCTGAGGACATCACAGCCATTGACACTCAATCGAGTGTGAGGATCAAAACTGCCGTAGGAAGAGGAGCTTATGTCAAAGACATTTATGTGAGAAGAATGACCTTGAAGACAATGAAGTATGTCTTTTGGATGACCGGCTCTTATGGATCGCACCCTGACCCCAGCTTTGATCCTAAAGCGTTGCCTGAGATTACAAACATCAACTACAAGCAGGTTGTGGCAGAAAATGTTACTTATTCCGCAAGGCTGGACGGAATCCCTAATGATCCTTTTAAGGGAATTTGCATTTCGAATGTGACCATCACACTAACTGAGAAGCCGAAAAAATTGCAATGGAACTGCACCAATGTTGAGGGAATCACAAGTAATGTGACCCCAAAGACGTGCGATTTGTTGCCCGAGCAGAAAGAAGTGGTTGATTGTGCTTTCCCTGAGGATAGGTTGGCGATCGAGGATGTTAAGTTGGTGACTTGCTCTGCAAGCACCCCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

473

Amino Acids

51.4

Weight (kDa)

6.08

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 144 - 425 4.9e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1123
AccII CGCG 1 cut(s) 912
AciI CCGC 5 cut(s) 71, 240, 260, 910, 1161
AclWI GGATC 4 cut(s) 971, 1075, 1085, 1181
AcoI YGGCCR 2 cut(s) 114, 490
AcsI RAATTY 3 cut(s) 17, 585, 1200
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 2 cut(s) 378, 569
AfiI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
AgeI ACCGGT 2 cut(s) 88, 467
AgsI TTSAA 5 cut(s) 199, 289, 520, 584, 1027
AjiI CACGTC 1 cut(s) 1305
AluBI AGCT 6 cut(s) 226, 250, 285, 923, 989, 1086
AluI AGCT 6 cut(s) 226, 250, 285, 923, 989, 1086
Alw21I GWGCWC 2 cut(s) 142, 831
Alw26I GTCTC 3 cut(s) 162, 434, 760
AlwI GGATC 4 cut(s) 971, 1075, 1085, 1181
Ama87I CYCGRG 1 cut(s) 1319
AoxI GGCC 4 cut(s) 114, 192, 362, 490
ApeKI GCWGC 3 cut(s) 203, 247, 642
ApoI RAATTY 3 cut(s) 17, 585, 1200
ArsI GACNNNNNNTTYG 4 cut(s) 1070, 1102, 1294, 1326
AsiGI ACCGGT 2 cut(s) 88, 467
Asp700I GAANNNNTTC 1 cut(s) 178
AspA2I CCTAGG 1 cut(s) 883
AspLEI GCGC 2 cut(s) 49, 247
AsuC2I CCSGG 2 cut(s) 264, 411
AsuHPI GGTGA 5 cut(s) 268, 442, 532, 852, 1399
AsuII TTCGAA 1 cut(s) 1211
AvaI CYCGRG 1 cut(s) 1319
AvrII CCTAGG 1 cut(s) 883
AxyI CCTNAGG 1 cut(s) 1353
BaeGI GKGCMC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 3 cut(s) 427, 747, 1034
Bbv12I GWGCWC 2 cut(s) 142, 831
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 3 cut(s) 190, 259, 654
BccI CCATC 4 cut(s) 636, 695, 783, 1229
BceAI ACGGC 7 cut(s) 146, 191, 253, 314, 493, 505, 959
BcgI CGANNNNNNTGC 2 cut(s) 1231, 1265
BcnI CCSGG 2 cut(s) 264, 411
BcoDI GTCTC 3 cut(s) 162, 434, 760
BfaI CTAG 2 cut(s) 297, 884
BfmI CTRYAG 1 cut(s) 124
BfuAI ACCTGC 1 cut(s) 1123
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 5 cut(s) 72, 204, 248, 260, 643
BlnI CCTAGG 1 cut(s) 883
BlsI GCNGC 5 cut(s) 73, 205, 249, 261, 644
BmcAI AGTACT 1 cut(s) 378
Bme1390I CCNGG 2 cut(s) 264, 411
BmeT110I CYCGRG 1 cut(s) 1319
BmgBI CACGTC 1 cut(s) 1305
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 264, 411
BmsI GCATC 4 cut(s) 239, 316, 394, 780
BpiI GAAGAC 3 cut(s) 427, 747, 1034
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 780
Bpu10I CCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 1211
BpuMI CCSGG 2 cut(s) 264, 411
BsaBI GATNNNNATC 1 cut(s) 560
BsaJI CCNNGG 5 cut(s) 263, 409, 493, 658, 883
BsaWI WCCGGW 3 cut(s) 88, 278, 467
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
Bse118I RCCGGY 3 cut(s) 88, 467, 1055
Bse1I ACTGG 2 cut(s) 374, 680
Bse21I CCTNAGG 1 cut(s) 1353
Bse3DI GCAATG 2 cut(s) 876, 1259
Bse8I GATNNNNATC 1 cut(s) 560
BseDI CCNNGG 5 cut(s) 263, 409, 493, 658, 883
BseGI GGATG 8 cut(s) 230, 331, 355, 628, 794, 799, 1056, 1381
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
BseMI GCAATG 2 cut(s) 876, 1259
BseMII CTCAG 5 cut(s) 359, 915, 1098, 1224, 1344
BseNI ACTGG 2 cut(s) 374, 680
BseRI GAGGAG 1 cut(s) 999
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 3 cut(s) 190, 259, 654
BseYI CCCAGC 2 cut(s) 49, 1082
BsgI GTGCAG 1 cut(s) 1246
Bsh1236I CGCG 1 cut(s) 912
Bsh1285I CGRYCG 1 cut(s) 1371
BshFI GGCC 4 cut(s) 116, 194, 364, 492
BshTI ACCGGT 2 cut(s) 88, 467
BsiEI CGRYCG 1 cut(s) 1371
BsiHKAI GWGCWC 2 cut(s) 142, 831
BsiHKCI CYCGRG 1 cut(s) 1319
BsiSI CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
BslI CCNNNNNNNGG 7 cut(s) 70, 95, 322, 389, 417, 618, 1196
BsmAI GTCTC 3 cut(s) 162, 434, 760
BsmBI CGTCTC 1 cut(s) 162
BsnI GGCC 4 cut(s) 116, 194, 364, 492
BsoBI CYCGRG 1 cut(s) 1319
Bsp119I TTCGAA 1 cut(s) 1211
Bsp1286I GDGCHC 4 cut(s) 101, 142, 666, 831
Bsp143I GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
BspACI CCGC 5 cut(s) 71, 240, 260, 910, 1161
BspANI GGCC 4 cut(s) 116, 194, 364, 492
BspCNI CTCAG 5 cut(s) 358, 916, 1099, 1225, 1345
BspFNI CGCG 1 cut(s) 912
BspLI GGNNCC 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 1123
BspPI GGATC 4 cut(s) 971, 1075, 1085, 1181
BspT104I TTCGAA 1 cut(s) 1211
BsrDI GCAATG 2 cut(s) 876, 1259
BsrFI RCCGGY 3 cut(s) 88, 467, 1055
BsrI ACTGG 2 cut(s) 374, 680
BssAI RCCGGY 3 cut(s) 88, 467, 1055
BssECI CCNNGG 5 cut(s) 263, 409, 493, 658, 883
BssMI GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
BssT1I CCWWGG 3 cut(s) 493, 658, 883
Bst4CI ACNGT 2 cut(s) 125, 869
Bst6I CTCTTC 2 cut(s) 317, 976
BstBI TTCGAA 1 cut(s) 1211
BstC8I GCNNGC 2 cut(s) 228, 1402
BstDEI CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 8 cut(s) 230, 331, 355, 628, 794, 799, 1056, 1381
BstFNI CGCG 1 cut(s) 912
BstHHI GCGC 2 cut(s) 49, 247
BstKTI GATC 8 cut(s) 105, 558, 564, 966, 1070, 1093, 1189, 1371
BstMAI GTCTC 3 cut(s) 162, 434, 760
BstMBI GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
BstMCI CGRYCG 1 cut(s) 1371
BstMWI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
BstSCI CCNGG 2 cut(s) 262, 409
BstSFI CTRYAG 1 cut(s) 124
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 3 cut(s) 190, 259, 654
BstV2I GAAGAC 3 cut(s) 427, 747, 1034
Bsu36I CCTNAGG 1 cut(s) 1353
BsuRI GGCC 4 cut(s) 116, 194, 364, 492
BtgZI GCGATG 1 cut(s) 757
BtrI CACGTC 1 cut(s) 1305
BtsCI GGATG 8 cut(s) 230, 331, 355, 628, 794, 799, 1056, 1381
BtsIMutI CAGTG 1 cut(s) 874
BveI ACCTGC 1 cut(s) 1123
Cac8I GCNNGC 2 cut(s) 228, 1402
CfoI GCGC 2 cut(s) 49, 247
Cfr10I RCCGGY 3 cut(s) 88, 467, 1055
Csp6I GTAC 2 cut(s) 377, 568
CspAI ACCGGT 2 cut(s) 88, 467
CviAII CATG 3 cut(s) 23, 553, 565
CviQI GTAC 2 cut(s) 377, 568
DdeI CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
DpnI GATC 8 cut(s) 104, 557, 563, 965, 1069, 1092, 1188, 1370
DpnII GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
DraIII CACNNNGTG 1 cut(s) 835
EaeI YGGCCR 2 cut(s) 114, 490
Eam1104I CTCTTC 2 cut(s) 317, 976
EarI CTCTTC 2 cut(s) 317, 976
EciI GGCGGA 1 cut(s) 255
Eco130I CCWWGG 3 cut(s) 493, 658, 883
Eco147I AGGCCT 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 1353
Eco88I CYCGRG 1 cut(s) 1319
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoT14I CCWWGG 3 cut(s) 493, 658, 883
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 3 cut(s) 493, 658, 883
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 3 cut(s) 26, 556, 568
FalI AAGNNNNNCTT 6 cut(s) 835, 867, 973, 1005, 1028, 1060
FatI CATG 3 cut(s) 22, 552, 564
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 5 cut(s) 72, 204, 248, 260, 643
FokI GGATG 8 cut(s) 217, 338, 362, 615, 801, 806, 1063, 1388
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 5 cut(s) 72, 204, 248, 260, 643
FspBI CTAG 2 cut(s) 297, 884
GlaI GCGC 2 cut(s) 48, 246
GluI GCNGC 5 cut(s) 72, 204, 248, 260, 643
GsaI CCCAGC 2 cut(s) 53, 1086
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 4 cut(s) 116, 194, 364, 492
HapII CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
HhaI GCGC 2 cut(s) 49, 247
Hin1II CATG 3 cut(s) 26, 556, 568
Hin6I GCGC 2 cut(s) 47, 245
HinP1I GCGC 2 cut(s) 47, 245
HindIII AAGCTT 1 cut(s) 283
HpaII CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
HphI GGTGA 5 cut(s) 268, 442, 532, 852, 1399
Hpy166II GTNNAC 1 cut(s) 725
Hpy188I TCNGA 6 cut(s) 235, 358, 440, 579, 815, 1418
Hpy188III TCNNGA 3 cut(s) 347, 559, 759
Hpy8I GTNNAC 1 cut(s) 725
Hpy99I CGWCG 4 cut(s) 239, 458, 491, 917
HpyAV CCTTC 5 cut(s) 205, 393, 411, 624, 837
HpyCH4III ACNGT 2 cut(s) 125, 869
HpyCH4IV ACGT 3 cut(s) 456, 915, 1304
HpyCH4V TGCA 9 cut(s) 230, 341, 677, 771, 855, 1206, 1252, 1263, 1400
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
HpyF3I CTNAG 6 cut(s) 218, 345, 924, 1107, 1233, 1353
HpySE526I ACGT 3 cut(s) 456, 915, 1304
Hsp92II CATG 3 cut(s) 26, 556, 568
HspAI GCGC 2 cut(s) 47, 245
Kzo9I GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
LmnI GCTCC 4 cut(s) 158, 242, 826, 986
Lsp1109I GCAGC 3 cut(s) 190, 259, 654
LweI GCATC 4 cut(s) 239, 316, 394, 780
MaeI CTAG 2 cut(s) 297, 884
MaeII ACGT 3 cut(s) 456, 915, 1304
MaeIII GTNAC 6 cut(s) 470, 527, 1150, 1216, 1291, 1387
MalI GATC 8 cut(s) 104, 557, 563, 965, 1069, 1092, 1188, 1370
MboI GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
MboII GAAGA 9 cut(s) 52, 304, 362, 427, 752, 993, 1026, 1039, 1405
MhlI GDGCHC 4 cut(s) 101, 142, 666, 831
MluCI AATT 7 cut(s) 17, 106, 462, 585, 607, 1200, 1247
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 4 cut(s) 145, 207, 258, 793
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 2 cut(s) 1194, 1380
MslI CAYNNNNRTG 2 cut(s) 324, 1007
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 6 cut(s) 89, 263, 279, 411, 468, 1056
MspR9I CCNGG 2 cut(s) 264, 411
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 4 cut(s) 200, 256, 878, 1401
NciI CCSGG 2 cut(s) 264, 411
NdeI CATATG 1 cut(s) 388
NdeII GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
NlaIII CATG 3 cut(s) 26, 556, 568
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 4 cut(s) 527, 1216, 1291, 1387
NspV TTCGAA 1 cut(s) 1211
PceI AGGCCT 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 178
PinAI ACCGGT 2 cut(s) 88, 467
PkrI GCNGC 5 cut(s) 73, 205, 249, 261, 644
Ple19I CGATCG 1 cut(s) 1371
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
PspFI CCCAGC 2 cut(s) 49, 1082
PspN4I GGNNCC 1 cut(s) 160
PvuI CGATCG 1 cut(s) 1371
RsaI GTAC 2 cut(s) 378, 569
RsaNI GTAC 2 cut(s) 377, 568
RseI CAYNNNNRTG 2 cut(s) 324, 1007
SaqAI TTAA 2 cut(s) 1194, 1380
SatI GCNGC 5 cut(s) 72, 204, 248, 260, 643
Sau3AI GATC 8 cut(s) 102, 555, 561, 963, 1067, 1090, 1186, 1368
ScaI AGTACT 1 cut(s) 378
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 264, 411
SduI GDGCHC 4 cut(s) 101, 142, 666, 831
SfaNI GCATC 4 cut(s) 239, 316, 394, 780
SfcI CTRYAG 1 cut(s) 124
SfuI TTCGAA 1 cut(s) 1211
SmiMI CAYNNNNRTG 2 cut(s) 324, 1007
Sse9I AATT 7 cut(s) 17, 106, 462, 585, 607, 1200, 1247
SseBI AGGCCT 1 cut(s) 194
SsiI CCGC 5 cut(s) 71, 240, 260, 910, 1161
SspMI CTAG 2 cut(s) 297, 884
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 262, 409
StyI CCWWGG 3 cut(s) 493, 658, 883
TaaI ACNGT 2 cut(s) 125, 869
TaiI ACGT 3 cut(s) 459, 918, 1307
TaqI TCGA 7 cut(s) 82, 486, 558, 738, 953, 1211, 1371
TaqII GACCGA 1 cut(s) 164
TasI AATT 7 cut(s) 17, 106, 462, 585, 607, 1200, 1247
TatI WGTACW 2 cut(s) 376, 567
TauI GCSGC 2 cut(s) 74, 262
Tru1I TTAA 2 cut(s) 1194, 1380
Tru9I TTAA 2 cut(s) 1194, 1380
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 4 cut(s) 527, 1216, 1291, 1387
TseI GCWGC 3 cut(s) 203, 247, 642
Tsp45I GTSAC 4 cut(s) 527, 1216, 1291, 1387
TspDTI ATGAA 3 cut(s) 375, 810, 1049
TspGWI ACGGA 4 cut(s) 186, 816, 839, 1188
TspRI CASTG 1 cut(s) 874
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 3 cut(s) 17, 585, 1200
XmaJI CCTAGG 1 cut(s) 883
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 2 cut(s) 297, 884
ZrmI AGTACT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.