pycom07g25650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004870
Physical Location & Seq
Forward (+)
25913711 .. 25914244
534 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25650.1

Sequence Viewer

Length: 534 bp
ATGGAGTTAGAAGAGCTAGAAAAAAGCTTCAAGGAGTTGAAGATCATCAACAGAAAGCAAGAAAAACGCGTCTGTTACCACGAGACAAGAGCTCGTAATTTAACCATCGGTTATCTTCTATTGCAAGCCTTATTTTTCCTAGGGTTTTCATCACGGCCGCCGCCGAACGGTTCGGATTCCGGTTACAGTTGCCAATGGTGGGTGCCCTTCAGCATGATACTGGTTTCTTCAGTAATATTCTTCATGGCTTTCCTAGACGCCGTGACAATGTTCTACCGGGTGCAGTATCATTTGGATGTCAACTATTTGGAGCAAGATCTCATATACAAGAAGATACAGGAGGCTAAGCAGCCACCCCCTAAGAGCCATGTGTCCATACAGTTAAAGGAGTGTCATACCCCTGGTGAAGTTATCAAAGTTGATGTTTTCCAGCTCTTGAAGCGCAAAGTTTACATTTACGTCACAACTTTGAGTTTGCTTGCTCTCACAGGACTTCAGTTGTATGTGTGCCGGTTGTTTTTCCACGCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.76

Weight (kDa)

8.99

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AccII CGCG 1 cut(s) 69
AciI CCGC 2 cut(s) 158, 161
AcoI YGGCCR 1 cut(s) 155
AcuI CTGAAG 3 cut(s) 193, 213, 479
AcyI GRCGYC 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 167, 199
AflIII ACRYGT 1 cut(s) 67
AgsI TTSAA 3 cut(s) 31, 40, 439
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 4 cut(s) 16, 27, 92, 433
AluI AGCT 4 cut(s) 16, 27, 92, 433
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 77
AoxI GGCC 1 cut(s) 155
ApeKI GCWGC 1 cut(s) 349
AspA2I CCTAGG 1 cut(s) 139
AspLEI GCGC 1 cut(s) 444
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 416
AvrII CCTAGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 94
BauI CACGAG 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 361
BccI CCATC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 170, 245
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 3 cut(s) 17, 140, 254
BglII AGATCT 1 cut(s) 316
BisI GCNGC 3 cut(s) 158, 161, 350
BlnI CCTAGG 1 cut(s) 139
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 3 cut(s) 159, 162, 351
Bme1390I CCNGG 2 cut(s) 278, 402
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 278, 402
Bpu1102I GCTNAGC 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 139, 400
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 199
Bse118I RCCGGY 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 139, 400
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 167, 199
BseNI ACTGG 1 cut(s) 225
BseSI GKGCMC 1 cut(s) 207
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 1 cut(s) 157
BshNI GGYRCC 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 3 cut(s) 180, 277, 511
BslI CCNNNNNNNGG 2 cut(s) 167, 199
BsmAI GTCTC 1 cut(s) 77
BsnI GGCC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 94, 207
Bsp143I GATC 2 cut(s) 42, 316
Bsp1720I GCTNAGC 1 cut(s) 345
BspACI CCGC 2 cut(s) 158, 161
BspANI GGCC 1 cut(s) 157
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 510
BsrI ACTGG 1 cut(s) 225
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 2 cut(s) 139, 400
BssMI GATC 2 cut(s) 42, 316
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 170, 188, 381
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 126, 480
BstDEI CTNAG 2 cut(s) 345, 360
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 2 cut(s) 45, 319
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 2 cut(s) 42, 316
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 1 cut(s) 439
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 276, 400
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 1 cut(s) 157
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 126, 480
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 510
CseI GACGC 2 cut(s) 58, 266
CviAII CATG 3 cut(s) 214, 244, 368
DdeI CTNAG 2 cut(s) 345, 360
DpnI GATC 2 cut(s) 44, 318
DpnII GATC 2 cut(s) 42, 316
EaeI YGGCCR 1 cut(s) 155
EagI CGGCCG 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Ecl136II GAGCTC 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 94
Eco52I CGGCCG 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 3 cut(s) 193, 213, 479
EcoICRI GAGCTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 3 cut(s) 217, 247, 371
FaiI YATR 9 cut(s) 215, 245, 323, 325, 369, 377, 396, 504, 532
FatI CATG 3 cut(s) 213, 243, 367
Fnu4HI GCNGC 3 cut(s) 158, 161, 350
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 3 cut(s) 158, 161, 350
FspBI CTAG 3 cut(s) 17, 140, 254
GlaI GCGC 1 cut(s) 443
GluI GCNGC 3 cut(s) 158, 161, 350
HaeIII GGCC 1 cut(s) 157
HapII CCGG 3 cut(s) 180, 277, 511
HgaI GACGC 2 cut(s) 58, 266
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 217, 247, 371
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 1 cut(s) 176
HpaII CCGG 3 cut(s) 180, 277, 511
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 2 cut(s) 301, 451
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 301, 451
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 3 cut(s) 170, 188, 381
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 2 cut(s) 124, 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 439
HpyF3I CTNAG 2 cut(s) 345, 360
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 217, 247, 371
HspAI GCGC 1 cut(s) 442
Kzo9I GATC 2 cut(s) 42, 316
LguI GCTCTTC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 9 cut(s) 193, 206, 290, 323, 387, 414, 443, 474, 524
Lsp1109I GCAGC 1 cut(s) 361
MaeI CTAG 3 cut(s) 17, 140, 254
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 4 cut(s) 74, 182, 262, 460
MalI GATC 2 cut(s) 44, 318
MboI GATC 2 cut(s) 42, 316
MboII GAAGA 6 cut(s) 23, 52, 107, 219, 232, 343
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 94, 207
MluCI AATT 1 cut(s) 97
MluI ACGCGT 1 cut(s) 67
MnlI CCTC 1 cut(s) 334
MseI TTAA 2 cut(s) 101, 383
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 3 cut(s) 180, 277, 511
MspR9I CCNGG 2 cut(s) 278, 402
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 439
NciI CCSGG 1 cut(s) 278
NdeII GATC 2 cut(s) 42, 316
NlaIII CATG 3 cut(s) 217, 247, 371
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 262, 460
PciSI GCTCTTC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 176
PkrI GCNGC 3 cut(s) 159, 162, 351
Psp124BI GAGCTC 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 204
PsuI RGATCY 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 294
SacI GAGCTC 1 cut(s) 94
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 2 cut(s) 101, 383
SatI GCNGC 3 cut(s) 158, 161, 350
Sau3AI GATC 2 cut(s) 42, 316
ScrFI CCNGG 2 cut(s) 278, 402
SduI GDGCHC 2 cut(s) 94, 207
SetI ASST 5 cut(s) 18, 29, 94, 435, 462
SmiMI CAYNNNNRTG 1 cut(s) 294
Sse9I AATT 1 cut(s) 97
SsiI CCGC 2 cut(s) 158, 161
SspI AATATT 1 cut(s) 237
SspMI CTAG 3 cut(s) 17, 140, 254
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 276, 400
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 3 cut(s) 170, 188, 381
TaiI ACGT 1 cut(s) 462
TasI AATT 1 cut(s) 97
TauI GCSGC 2 cut(s) 160, 163
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 2 cut(s) 101, 383
Tru9I TTAA 2 cut(s) 101, 383
TseFI GTSAC 2 cut(s) 262, 460
TseI GCWGC 1 cut(s) 349
Tsp45I GTSAC 2 cut(s) 262, 460
TspDTI ATGAA 2 cut(s) 138, 232
XmaJI CCTAGG 1 cut(s) 139
XspI CTAG 3 cut(s) 17, 140, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.