Rorug05G0539800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004870
Physical Location & Seq
Forward (+)
72817592 .. 72817855
264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0539800.1

Sequence Viewer

Length: 264 bp
ATGATGATCAAGAAGCAGTGCGCCTCGATCCCTAATGCGATGAAGCGTTACCACACGCACAGGGTGGGCCCTAACAAGTGCCGCTCCGTTGTGACGCAGGATATTGCTGCTCCCGTCTCCACTGTGTGGTCGATGGTCAGGTCAGGCGCATCGACAAGCCCCAAGCCTACAAGTACTTTGTCAAGAGCTGCCACATCATCGTTGGGGACTCGAGATCAGCACCCTCCGCGAGGTCCAGGTCATCTCGAGGCTTCTGGCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

87

Amino Acids

9.3

Weight (kDa)

10.97

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 1 cut(s) 229
AciI CCGC 2 cut(s) 82, 227
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 256
AdeI CACNNNGTG 2 cut(s) 64, 126
AfaI GTAC 1 cut(s) 175
AfiI CCNNNNNNNGG 2 cut(s) 126, 230
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 210, 245
AoxI GGCC 2 cut(s) 67, 256
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 107, 188
AspLEI GCGC 2 cut(s) 23, 149
AspS9I GGNCC 3 cut(s) 67, 68, 233
AvaI CYCGRG 2 cut(s) 210, 245
AvaII GGWCC 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 71
BalI TGGCCA 1 cut(s) 258
BanII GRGCYC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 94, 175
BccI CCATC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 121
BisI GCNGC 3 cut(s) 82, 108, 189
BlsI GCNGC 3 cut(s) 83, 109, 190
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 233
BmeT110I CYCGRG 2 cut(s) 210, 245
BmgT120I GGNCC 3 cut(s) 67, 68, 233
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 230
BseBI CCWGG 1 cut(s) 237
BseLI CCNNNNNNNGG 2 cut(s) 126, 230
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 2 cut(s) 94, 175
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 69, 258
BsiHKCI CYCGRG 2 cut(s) 210, 245
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 126, 230
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 2 cut(s) 69, 258
BsoBI CYCGRG 2 cut(s) 210, 245
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 6, 27, 214
BspACI CCGC 2 cut(s) 82, 227
BspANI GGCC 2 cut(s) 69, 258
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 84
BssMI GATC 3 cut(s) 6, 27, 214
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 124
BstENI CCTNNNNNAGG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 2 cut(s) 23, 149
BstKTI GATC 3 cut(s) 9, 30, 217
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 6, 27, 214
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 94, 175
BsuRI GGCC 2 cut(s) 69, 258
BtgZI GCGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 2 cut(s) 23, 120
CfoI GCGC 2 cut(s) 23, 149
Cfr13I GGNCC 3 cut(s) 67, 68, 233
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 174
CviJI RGCY 6 cut(s) 69, 159, 166, 188, 251, 258
CviKI_1 RGCY 6 cut(s) 69, 159, 166, 188, 251, 258
CviQI GTAC 1 cut(s) 174
DpnI GATC 3 cut(s) 8, 29, 216
DpnII GATC 3 cut(s) 6, 27, 214
DraIII CACNNNGTG 2 cut(s) 64, 126
EaeI YGGCCR 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 233
Eco88I CYCGRG 2 cut(s) 210, 245
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 121
FaqI GGGAC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 82, 108, 189
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 3 cut(s) 82, 108, 189
GlaI GCGC 2 cut(s) 22, 148
GluI GCNGC 3 cut(s) 82, 108, 189
HaeIII GGCC 2 cut(s) 69, 258
HgaI GACGC 1 cut(s) 103
HhaI GCGC 2 cut(s) 23, 149
Hin6I GCGC 2 cut(s) 21, 147
HinP1I GCGC 2 cut(s) 21, 147
HinfI GANTC 1 cut(s) 208
Hpy188III TCNNGA 4 cut(s) 10, 183, 212, 245
HpyCH4III ACNGT 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HspAI GCGC 2 cut(s) 21, 147
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 3 cut(s) 6, 27, 214
LmnI GCTCC 2 cut(s) 89, 115
LpnPI CCDG 7 cut(s) 46, 83, 124, 129, 222, 240, 249
Lsp1109I GCAGC 2 cut(s) 94, 175
LweI GCATC 1 cut(s) 158
MaeIII GTNAC 2 cut(s) 47, 91
MalI GATC 3 cut(s) 8, 29, 216
MbiI CCGCTC 1 cut(s) 84
MboI GATC 3 cut(s) 6, 27, 214
MhlI GDGCHC 1 cut(s) 71
MlsI TGGCCA 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 4 cut(s) 34, 224, 234, 241
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 3 cut(s) 6, 27, 214
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 91
PaeR7I CTCGAG 2 cut(s) 210, 245
PflMI CCANNNNNTGG 1 cut(s) 126
PkrI GCNGC 3 cut(s) 83, 109, 190
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 69
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 3 cut(s) 67, 68, 233
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
SatI GCNGC 3 cut(s) 82, 108, 189
Sau3AI GATC 3 cut(s) 6, 27, 214
Sau96I GGNCC 3 cut(s) 67, 68, 233
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 1 cut(s) 71
SetI ASST 4 cut(s) 143, 190, 235, 241
SfaNI GCATC 1 cut(s) 158
Sfr274I CTCGAG 2 cut(s) 210, 245
SinI GGWCC 1 cut(s) 233
SlaI CTCGAG 2 cut(s) 210, 245
SmlI CTYRAG 2 cut(s) 210, 245
SmoI CTYRAG 2 cut(s) 210, 245
SsiI CCGC 2 cut(s) 82, 227
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 124
TaqI TCGA 5 cut(s) 26, 131, 152, 211, 246
TatI WGTACW 1 cut(s) 173
TauI GCSGC 1 cut(s) 84
TscAI CASTG 2 cut(s) 23, 127
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 2 cut(s) 107, 188
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 23, 127
Van91I CCANNNNNTGG 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XhoI CTCGAG 2 cut(s) 210, 245
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.