pycom08g08500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001728
Physical Location & Seq
Forward (+)
6790398 .. 6790721
324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08500.1

Sequence Viewer

Length: 324 bp
ATGCCGGAAGCTCTAATGGCGGAGATCAACACAGCAATTTGCAATCTCGAATACGCACGCGCCACCGATCTTCTCGGCTCTCCGTCTTCTTCGTCTTCGATGGCGAAAACCGAGAGCTCTGATCAACCCGTATTCAATGCAAGAATGGCGGACCAAGCATACAAGGCAGGCTGCGCAGCATTGGCAGCTGGGAAGCTCGACGAGGCCCTTCTCTCTCTAAACACTTCTCTCTCTAAGTGCCCACCTGACCAGACCTCTGCCGTGGCCAAACTGCACTCTTTGATCTCTCTCACATCTCAGCAGCTCCACAAGCCCCCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

108

Amino Acids

11.18

Weight (kDa)

5.53

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 175
AccII CGCG 1 cut(s) 60
AciI CCGC 2 cut(s) 20, 149
AcoI YGGCCR 1 cut(s) 264
AgsI TTSAA 1 cut(s) 136
AluBI AGCT 6 cut(s) 11, 117, 188, 196, 304, 321
AluI AGCT 6 cut(s) 11, 117, 188, 196, 304, 321
Alw21I GWGCWC 1 cut(s) 119
AoxI GGCC 2 cut(s) 204, 264
ApeKI GCWGC 4 cut(s) 171, 176, 185, 301
AspLEI GCGC 2 cut(s) 62, 176
AspS9I GGNCC 2 cut(s) 151, 205
AvaII GGWCC 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 1 cut(s) 266
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 2 cut(s) 78, 87
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 4 cut(s) 158, 188, 197, 313
BccI CCATC 1 cut(s) 94
BceAI ACGGC 1 cut(s) 245
BclI TGATCA 1 cut(s) 121
BisI GCNGC 4 cut(s) 172, 177, 186, 302
BlsI GCNGC 4 cut(s) 173, 178, 187, 303
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 151, 205
BpiI GAAGAC 2 cut(s) 78, 87
BsaJI CCNNGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 4 cut(s) 158, 188, 197, 313
BseYI CCCAGC 2 cut(s) 188, 317
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 206, 266
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 1 cut(s) 5
BsnI GGCC 2 cut(s) 206, 266
Bsp1286I GDGCHC 2 cut(s) 119, 242
Bsp143I GATC 4 cut(s) 24, 67, 121, 282
BspACI CCGC 2 cut(s) 20, 149
BspANI GGCC 2 cut(s) 206, 266
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 4 cut(s) 24, 67, 121, 282
BstC8I GCNNGC 2 cut(s) 58, 169
BstDEI CTNAG 2 cut(s) 234, 297
BstDSI CCRYGG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 62, 176
BstKTI GATC 4 cut(s) 27, 70, 124, 285
BstMBI GATC 4 cut(s) 24, 67, 121, 282
BstMWI GCNNNNNNNGC 8 cut(s) 17, 146, 155, 164, 173, 182, 185, 310
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 4 cut(s) 158, 188, 197, 313
BstV2I GAAGAC 2 cut(s) 78, 87
BsuRI GGCC 2 cut(s) 206, 266
BtgI CCRYGG 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 58, 169
CfoI GCGC 2 cut(s) 62, 176
Cfr13I GGNCC 2 cut(s) 151, 205
DdeI CTNAG 2 cut(s) 234, 297
DpnI GATC 4 cut(s) 26, 69, 123, 284
DpnII GATC 4 cut(s) 24, 67, 121, 282
EaeI YGGCCR 1 cut(s) 264
EciI GGCGGA 2 cut(s) 35, 164
Ecl136II GAGCTC 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 119
FaiI YATR 1 cut(s) 160
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 172, 177, 186, 302
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 4 cut(s) 172, 177, 186, 302
FspI TGCGCA 1 cut(s) 175
GlaI GCGC 2 cut(s) 61, 175
GluI GCNGC 4 cut(s) 172, 177, 186, 302
GsaI CCCAGC 2 cut(s) 192, 321
HaeIII GGCC 2 cut(s) 206, 266
HapII CCGG 1 cut(s) 5
HhaI GCGC 2 cut(s) 62, 176
Hin6I GCGC 2 cut(s) 60, 174
HinP1I GCGC 2 cut(s) 60, 174
HpaII CCGG 1 cut(s) 5
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 1 cut(s) 218
HpyCH4V TGCA 3 cut(s) 42, 140, 274
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 146, 155, 164, 173, 182, 185, 310
HpyF3I CTNAG 2 cut(s) 234, 297
HspAI GCGC 2 cut(s) 60, 174
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 4 cut(s) 24, 67, 121, 282
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 5 cut(s) 18, 153, 174, 258, 263
Lsp1109I GCAGC 4 cut(s) 158, 188, 197, 313
MalI GATC 4 cut(s) 26, 69, 123, 284
MboI GATC 4 cut(s) 24, 67, 121, 282
MboII GAAGA 4 cut(s) 62, 78, 81, 87
MhlI GDGCHC 2 cut(s) 119, 242
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 266
MnlI CCTC 2 cut(s) 196, 265
Mox20I TGGCCA 1 cut(s) 266
MscI TGGCCA 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 188, 321
MspI CCGG 1 cut(s) 5
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 8 cut(s) 17, 146, 155, 164, 173, 182, 185, 310
NdeII GATC 4 cut(s) 24, 67, 121, 282
NmeAIII GCCGAG 1 cut(s) 54
NsbI TGCGCA 1 cut(s) 175
PkrI GCNGC 4 cut(s) 173, 178, 187, 303
Psp124BI GAGCTC 1 cut(s) 119
PspFI CCCAGC 2 cut(s) 188, 317
PspPI GGNCC 2 cut(s) 151, 205
PvuII CAGCTG 2 cut(s) 188, 321
SacI GAGCTC 1 cut(s) 119
SatI GCNGC 4 cut(s) 172, 177, 186, 302
Sau3AI GATC 4 cut(s) 24, 67, 121, 282
Sau96I GGNCC 2 cut(s) 151, 205
SduI GDGCHC 2 cut(s) 119, 242
SetI ASST 8 cut(s) 13, 119, 190, 198, 247, 257, 306, 323
SinI GGWCC 1 cut(s) 151
Sse9I AATT 1 cut(s) 36
SsiI CCGC 2 cut(s) 20, 149
SstI GAGCTC 1 cut(s) 119
TaqI TCGA 3 cut(s) 48, 98, 198
TasI AATT 1 cut(s) 36
TseI GCWGC 4 cut(s) 171, 176, 185, 301
TspGWI ACGGA 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.