Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

14798362 .. 14808391

Gene Structure 10030 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4569 bp

ATGACAAACATTTGGACAATTTGCATTAATGGTGAAGGGTTGGTCGACTGCACCACCCAACTCACAGTCAAGACACGCCACCGGCTCCAAAATTTAATTCATTCATTCATCATTCCTTATGGAATGTCCTGCTTCCGTATTTACAAGATCCCATCAAAATCCACATGTGAAAATTTGTTTAAGCCCAAAAAATTCACACAAGAAAAACAGAGAGAGGGAAAATCACTTGACTGGTTAGAGGAGGGAGAGAGAGAGAGAGAGAGAGAGCCCACCTTGATCTTAATTAGAAATGGCAGCATGTCAGAAGCCCAGAGGAGATTGGTGACAGTGCCCAGTTTCCATCGTGGAAATGGGTACGCAAATGGTGTACTCAGGCATCCTGCTACAGCATCTAAAGTTGATGAATTCTGCTATGCCCTTGGTGGGAAGAAGCCAATTCATAGCATTTTAATTGCAAACAACGGAATGGCAGCTGTCAAGTTTATTCGTAGTGTGAGGACATGGTCTTATGAAACATTTGGTACAGAGAAGGCTGTTTTATTGGTGGCAATGGCCACACCAGAGGACATGCGAATCAATGCAGAACACATCAGAATTGCCGATCAGTTTGTGGAGGTTCCTGGTGGAACTAACAATAATAACTATGCCAATGTGCAACTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCCTCAGAGAATCCTGAGCTTCCAGATGCATTGGATGCAAAGGGAATTGTATTTCTTGGGCCACCATCTATATCTATGGCAGCATTAGGAGATAAAATCGGTTCATCATTAATTGCTCAGGCTGCCAACGTACCTACTCTTCCATGGAGTGGATCACATGTGAAAATTCCTTCAGAAAGCTGCTTGGTTACAATCCCGGATGAAGTATATAGGGAAGCGTGTGTTTATACAACAGAGGAAGCAGTTGCAAGTTGTCAAGTTGTAGGTTACCCAGCAATGATTAAGGCTTCATGGGGTGGAGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAGGTAAGGGCATTGTTCAAGCAAGTTCAGGGTGAAGTTCCAGGATCTCCAATTTTTATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAGGTACAGTTACTGTGTGATCAGCATGGAAATGTTGCAGCTTTTCATAGCCGTGACTGCAGTGTTCAAAGGCGGCACCAGAAGATTATTGAGGAGGGTCCAATTACTGTAGCTCCTATAGAGACAGTAAAAAAGTTGGAGCAATCAGCTCGAAGGTTGGCTAAATCTGTTAATTACGTTGGAGCAGCTACTGTTGAGTATCTCTACAGTATGGAAACTGGCGAGTACTATTTCTTAGAGCTGAACCCTCGATTACAGGTGGAGCACCCAGTCACTGAGTGGATAGCTGAAATAAATCTCCCAGCAGCCCAAGTTGTAGTTGGGATGGGCATACCTCTCTGGCAAATCCCTGAAATAAGGCGATTTTATGGAATGGAACATGGTGGTGGATATGATGCTTGGAGAAAAACTTCAGCTAGTGCTGCCCCATTCGATTTTGACAAGGCAGAATCTACAAGGCCAAAGGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAGCCTACCAGTGGGAAAGTACAGGAGTTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCATACTTTTCTGTTAAGTCTGGAGGGGGCATTCATGAATTCTCGGACTCTCAGTTTGGACATGTTTTTGCATTTGGGGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTTGATTATTCAATCGATCTTCTACATGCCTCAGACTACAGAGAGAATAAAATCCACACGGGTTGGTTGGATAGTAGAATCGCCATGCGAGTTAGAGCAGAAAGGCCTCCTTGGTATCTTTCTGTAGTTGGAGGGACTCTGTTTAAAGCGTCTACCAGTAGTGCAGCAATGGTTTCAGATTATGTCGGTTATCTTGAGAAAGGGCAAATTCCACCCAAGCATATATCACTTGTCCATGCACAAGTATCGTTGAACATTGAAGGGAGCAAATACACGATTGACATGGTGAGAGGGGGTCCTGGAAGCTACAGATTGAGGATGAATGAATCAGAGATTGAAGCAGAGATACATACTTTACGTGATGGGGGTTTGTTGATGCAGTTGGACGGAAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGCCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGACCATGACCCATCAAAGTTAATAGCAGAGACACCATGCAAGCTGCTGAGGTACCTGGTTGCTGATAGTAGTCATGTTGATGCTGACACACCATATGCTGAGGTTGAGGTTATGAAGATGTGTATGCCTCTTCTTTCACCTGCTTCTGGAGCTATCCACTTTAGAATATCTGAAGGTCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCAGCCGTAAGAAAGGCAGAACCTTTCCATGGGAGCTTCCCGGTTCTGGGGCCCCCAACTGCAATTTCTGGTAAAGTTCACCAGAGATGTGCTGCTAGCATAACTGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCACTTGCCTAGACAATCCTGAACTCCCTTTCCTTCAATGGCAAGAGTGCTTTGCTGTTCTAGCAACCCGCCTCCCCAAAGATCTTAAAAATGAATTGGAATCAAAATTTAAGGAGTTTGAGGTGATTTCTAGTTCTCAGAATGTTGAGTTCCCTGCTAAGTTGCTGCGCGTTGTTCTTGAGGCCCATCTATTCTCCTGTCCTGATAAAGAGAAAGGAGCCCAAGAAAGGCTTGTTGAGCCTTTGATGAGCCTTGTGAAGTCTTATGAGGGTGGAAGAGAAAGTCATGCCCGAGTTATCATTCATTCCCTTTTTGAAGAGTACCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTAATTGAACACCTTCGACTTCTGTATAAAAAAGATCTATTGAAGATTGTGGACATTGTGCTTTCTCACCAGGGTGTCAAGAATAAAAATAAGTTGATACTACAACTCATGGAACAACTGGTCTACCCCAACCCTGCTGCGTACAGGGAAAAGCTAATTCGGTTTTCTCAACTTAACCATACAAGCTATTCTGAGTTGGCACTTAAGGCAAGTCAACTGATGGAGCAAACCAAGTTGAGTGAACTTCGTTCCAGCATTGCCAGAAGTCTTTCTGAGTTAGAGATGTTTACCGAGGATGGTGAAAGCATGGATACTCCTAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGCGCCCCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTATTTGATCACAGTGATCACACACTTCAGAGGCGGGTTGTGGAGAGCTATGTGCGTCGGTTATACCAGCCATATCTTTTAAAGGTGAGCGTGAGGATGCAGTGGCAAAGATCTGGTCTTATGGCTTCCTGGGAGTTCTTGGACGAACATACAGAGAGAAAAACTGCGAATGAAGATCAATCTTCTGATAAATTAATTGAGAAATACAACGAGAGAAAATGGGGAGTCATGGTTATTATCAAATCCCTTCAATTTCTGCCAGCAGTTATTAATGCTGCACTGAAGGAAATGTCTCATCAACTTCATGAATCAATTCCAAATGGATCTAGTGAACCAAGTGGCTTTGGTAATATGATGCATATTGCTTTAGTTGGCATCAACAATCAGATGAGTTTACTTCAGGATAGTGGTGATGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATTCTTAAGGAACAAGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCAGGGTAATTAGTTGCATCATACAAAGAGATGAAGGGCGAGCTCCTATGAGGCATTCCTTCCACTGGTCATCAGAAAAACTCTATTACGAGGAAGAACCTTTATTGCGTCATCTGGAGCCTCCTCTATCCATTTACCTTGAATTGGACAAGCTTAAAGGTTATGAGAATGTACAGTATACCCCATCCCGGGACCGTCAATGGCACTTGTACAGCGTTGTAGACAAGCCGCAACCAACCAAAAGGATGTTCCTCAGAACACTTGTAAGGCAGCCCACTTCAAATGATGTGTTCGCTGGATTTCAAAGGCTAGACATGGAAGCAGCTAGTAAGCAATGGGCTTTGTCATTTACTTCAAGGAGCATTTTGAGGTCCTTATTGACAGCAATGGAAGAGTTGGAACTTAATGCACACAATGCCACTGTCAAATCTGACCATACTCATATGTACCTTTATATCTTACGTGAGCAACAAATTCAAGATCGATCTACTGCCTTACTCCAAGAGAGTTGGTTTAGATGCTGGACAAGAAGAAATTGTGGTGGAGGCGATCTTAGAAGAACTTGCACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

1523

Amino Acids

171.26

Weight (kDa)

6.28

Isoelectric Point (pI)

43.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	146 - 264	3e-29	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	313 - 495	4.6e-45	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	314 - 465	7.8e-08	ATP-grasp domain
ATP-grasp_3	PF02655	321 - 467	7.7e-08	ATP-grasp domain
Biotin_carb_C	PF02785	542 - 648	1.4e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	664 - 779	2.1e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	789 - 852	6e-09	Biotin-requiring enzyme
ACC_central	PF08326	853 - 1492	7.9e-156	Acetyl-CoA carboxylase, central region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1112
AarI	CACCTGC	2 cut(s)	2491, 3983
Acc36I	ACCTGC	2 cut(s)	2491, 3983
Acc65I	GGTACC	1 cut(s)	2394
AccB1I	GGYRCC	2 cut(s)	1218, 2394
AccB7I	CCANNNNNTGG	2 cut(s)	866, 1652
AccI	GTMKAC	6 cut(s)	45, 2024, 3225, 3982, 4175, 4218
AccII	CGCG	1 cut(s)	2907
AciI	CCGC	4 cut(s)	1216, 2806, 3532, 4226
AclWI	GGATC	9 cut(s)	142, 877, 1105, 1637, 1650, 2561, 3462, 3829, 3924
AcoI	YGGCCR	1 cut(s)	552
AcuI	CTGAAG	9 cut(s)	873, 1539, 1856, 2321, 2535, 2556, 3509, 3800, 3881
AcvI	CACGTG	1 cut(s)	4566
AdeI	CACNNNGTG	1 cut(s)	1422
AflII	CTTAAG	2 cut(s)	3305, 3956
AflIII	ACRYGT	4 cut(s)	164, 685, 874, 1777
AjnI	CCWGG	7 cut(s)	619, 703, 1093, 2170, 2397, 3171, 3626
AjuI	GAANNNNNNNTTGG	6 cut(s)	81, 113, 2620, 2652, 4229, 4261
AleI	CACNNNNGTG	1 cut(s)	3174
AloI	GAACNNNNNNTCC	2 cut(s)	2751, 2783
Alw21I	GWGCWC	3 cut(s)	1410, 1807, 4042
Alw26I	GTCTC	4 cut(s)	1259, 1803, 2366, 3795
AlwI	GGATC	9 cut(s)	142, 877, 1105, 1637, 1650, 2561, 3462, 3829, 3924
AlwNI	CAGNNNCTG	3 cut(s)	1156, 1334, 1418
Ama87I	CYCGRG	3 cut(s)	1800, 3027, 4185
AoxI	GGCC	8 cut(s)	552, 701, 712, 776, 1601, 1976, 2621, 2919
ApaI	GGGCCC	1 cut(s)	2625
ArsI	GACNNNNNNTTYG	4 cut(s)	1754, 1786, 3598, 3630
AseI	ATTAAT	4 cut(s)	27, 827, 3692, 3768
Asp700I	GAANNNNTTC	4 cut(s)	1552, 3114, 3370, 3810
Asp718I	GGTACC	1 cut(s)	2394
AspLEI	GCGC	2 cut(s)	2907, 3467
AsuC2I	CCSGG	4 cut(s)	914, 2612, 4186, 4187
AsuNHI	GCTAGC	1 cut(s)	2666
AvaI	CYCGRG	3 cut(s)	1800, 3027, 4185
AvaII	GGWCC	5 cut(s)	1039, 1241, 2168, 4189, 4368
BaeGI	GKGCMC	2 cut(s)	333, 2625
BalI	TGGCCA	1 cut(s)	554
BamHI	GGATCC	1 cut(s)	1642
BanI	GGYRCC	2 cut(s)	1218, 2394
BanII	GRGCYC	5 cut(s)	270, 1807, 2625, 2959, 4042
BarI	GAAGNNNNNNTAC	4 cut(s)	840, 872, 2202, 2234
BbrPI	CACGTG	1 cut(s)	4566
Bbv12I	GWGCWC	3 cut(s)	1410, 1807, 4042
BbvCI	CCTCAGC	2 cut(s)	2390, 2442
BceAI	ACGGC	2 cut(s)	1179, 2561
BcgI	CGANNNNNNTGC	4 cut(s)	1274, 1308, 2100, 2134
BciT130I	CCWGG	7 cut(s)	621, 705, 1095, 2172, 2399, 3173, 3628
BciVI	GTATCC	1 cut(s)	3406
BclI	TGATCA	3 cut(s)	1162, 3505, 3514
BcnI	CCSGG	4 cut(s)	914, 2612, 4186, 4187
BcoDI	GTCTC	4 cut(s)	1259, 1803, 2366, 3795
BfaI	CTAG	9 cut(s)	1560, 1808, 2667, 2747, 2798, 2868, 3825, 4307, 4323
BfmI	CTRYAG	9 cut(s)	384, 1201, 1251, 1260, 1348, 1909, 1995, 2179, 2676
BfoI	RGCGCY	1 cut(s)	3468
BfrI	CTTAAG	2 cut(s)	3305, 3956
BfuAI	ACCTGC	2 cut(s)	2491, 3983
BfuI	GTATCC	1 cut(s)	3406
BglI	GCCNNNNNGGC	1 cut(s)	3473
BglII	AGATCT	3 cut(s)	2818, 3136, 3608
BmcAI	AGTACT	1 cut(s)	1370
Bme18I	GGWCC	5 cut(s)	1039, 1241, 2168, 4189, 4368
BmeT110I	CYCGRG	3 cut(s)	1800, 3027, 4185
BmrI	ACTGGG	2 cut(s)	327, 1406
BmtI	GCTAGC	1 cut(s)	2670
BmuI	ACTGGG	2 cut(s)	327, 1406
BpmI	CTGGAG	4 cut(s)	1758, 2511, 3961, 4133
Bpu10I	CCTNAGC	4 cut(s)	732, 834, 2390, 2442
BpuEI	CTTGAG	3 cut(s)	2087, 2936, 3909
BpuMI	CCSGG	4 cut(s)	914, 2612, 4186, 4187
Bsa29I	ATCGAT	2 cut(s)	1887, 4480
BsaAI	YACGTR	3 cut(s)	2231, 4460, 4566
BsaBI	GATNNNNATC	1 cut(s)	3915
BsaJI	CCNNGG	8 cut(s)	418, 860, 1982, 2599, 3172, 3393, 3627, 4185
BsaXI	ACNNNNNCTCC	4 cut(s)	1240, 1270, 3714, 3744
Bse118I	RCCGGY	1 cut(s)	81
Bse1I	ACTGG	9 cut(s)	236, 333, 1366, 1412, 1668, 2028, 3225, 3978, 4067
Bse3DI	GCAATG	7 cut(s)	555, 999, 2046, 2532, 3357, 4337, 4389
Bse8I	GATNNNNATC	1 cut(s)	3915
BseBI	CCWGG	7 cut(s)	621, 705, 1095, 2172, 2399, 3173, 3628
BseCI	ATCGAT	2 cut(s)	1887, 4480
BseDI	CCNNGG	8 cut(s)	418, 860, 1982, 2599, 3172, 3393, 3627, 4185
BseJI	GATNNNNATC	1 cut(s)	3915
BseMI	GCAATG	7 cut(s)	555, 999, 2046, 2532, 3357, 4337, 4389
BseNI	ACTGG	9 cut(s)	236, 333, 1366, 1412, 1668, 2028, 3225, 3978, 4067
BseRI	GAGGAG	5 cut(s)	254, 328, 1250, 1869, 4110
BseSI	GKGCMC	2 cut(s)	333, 2625
BseYI	CCCAGC	2 cut(s)	988, 1444
BsgI	GTGCAG	4 cut(s)	34, 2055, 3759, 4011
Bsh1236I	CGCG	1 cut(s)	2907
BshFI	GGCC	8 cut(s)	554, 703, 714, 778, 1603, 1978, 2623, 2921
BshNI	GGYRCC	2 cut(s)	1218, 2394
BshVI	ATCGAT	2 cut(s)	1887, 4480
BsiHKAI	GWGCWC	3 cut(s)	1410, 1807, 4042
BsiHKCI	CYCGRG	3 cut(s)	1800, 3027, 4185
BsiSI	CCGG	4 cut(s)	82, 914, 2612, 4186
BslFI	GGGAC	2 cut(s)	2020, 4202
BsmAI	GTCTC	4 cut(s)	1259, 1803, 2366, 3795
BsmFI	GGGAC	2 cut(s)	2020, 4202
BsmI	GAATGC	2 cut(s)	1746, 4051
BsnI	GGCC	8 cut(s)	554, 703, 714, 778, 1603, 1978, 2623, 2921
BsoBI	CYCGRG	3 cut(s)	1800, 3027, 4185
Bsp120I	GGGCCC	1 cut(s)	2621
Bsp1286I	GDGCHC	7 cut(s)	270, 333, 1410, 1807, 2625, 2959, 4042
Bsp1407I	TGTACA	2 cut(s)	4168, 4206
Bsp19I	CCATGG	2 cut(s)	860, 2599
BspACI	CCGC	4 cut(s)	1216, 2806, 3532, 4226
BspANI	GGCC	8 cut(s)	554, 703, 714, 778, 1603, 1978, 2623, 2921
BspDI	ATCGAT	2 cut(s)	1887, 4480
BspFNI	CGCG	1 cut(s)	2907
BspHI	TCATGA	2 cut(s)	1750, 3802
BspMAI	CTGCAG	2 cut(s)	1205, 2680
BspMI	ACCTGC	2 cut(s)	2491, 3983
BspOI	GCTAGC	1 cut(s)	2670
BspPI	GGATC	9 cut(s)	142, 877, 1105, 1637, 1650, 2561, 3462, 3829, 3924
BspT107I	GGYRCC	2 cut(s)	1218, 2394
BspTI	CTTAAG	2 cut(s)	3305, 3956
BsrDI	GCAATG	7 cut(s)	555, 999, 2046, 2532, 3357, 4337, 4389
BsrFI	RCCGGY	1 cut(s)	81
BsrGI	TGTACA	2 cut(s)	4168, 4206
BsrI	ACTGG	9 cut(s)	236, 333, 1366, 1412, 1668, 2028, 3225, 3978, 4067
BssAI	RCCGGY	1 cut(s)	81
BssECI	CCNNGG	8 cut(s)	418, 860, 1982, 2599, 3172, 3393, 3627, 4185
BssNAI	GTATAC	1 cut(s)	4176
BssT1I	CCWWGG	4 cut(s)	418, 860, 1982, 2599
Bst1107I	GTATAC	1 cut(s)	4176
Bst2UI	CCWGG	7 cut(s)	621, 705, 1095, 2172, 2399, 3173, 3628
Bst6I	CTCTTC	5 cut(s)	861, 2478, 3007, 3048, 4383
BstAFI	CTTAAG	2 cut(s)	3305, 3956
BstAPI	GCANNNNNTGC	3 cut(s)	752, 2734, 4412
BstAUI	TGTACA	2 cut(s)	4168, 4206
BstBAI	YACGTR	3 cut(s)	2231, 4460, 4566
BstC8I	GCNNGC	7 cut(s)	1703, 2333, 2384, 2532, 2668, 3759, 4038
BstDSI	CCRYGG	2 cut(s)	860, 2599
BstEII	GGTNACC	1 cut(s)	983
BstFNI	CGCG	1 cut(s)	2907
BstH2I	RGCGCY	1 cut(s)	3468
BstHHI	GCGC	2 cut(s)	2907, 3467
BstMAI	GTCTC	4 cut(s)	1259, 1803, 2366, 3795
BstNI	CCWGG	7 cut(s)	621, 705, 1095, 2172, 2399, 3173, 3628
BstNSI	RCATGY	7 cut(s)	168, 301, 571, 689, 878, 1781, 1901
BstPI	GGTNACC	1 cut(s)	983
BstSFI	CTRYAG	9 cut(s)	384, 1201, 1251, 1260, 1348, 1909, 1995, 2179, 2676
BstSLI	GKGCMC	2 cut(s)	333, 2625
BstUI	CGCG	1 cut(s)	2907
BstX2I	RGATCY	8 cut(s)	147, 1097, 1642, 2818, 3136, 3454, 3608, 3821
BstXI	CCANNNNNNTGG	1 cut(s)	1713
BstYI	RGATCY	8 cut(s)	147, 1097, 1642, 2818, 3136, 3454, 3608, 3821
BstZ17I	GTATAC	1 cut(s)	4176
Bsu15I	ATCGAT	2 cut(s)	1887, 4480
BsuI	GTATCC	1 cut(s)	3406
BsuRI	GGCC	8 cut(s)	554, 703, 714, 778, 1603, 1978, 2623, 2921
BsuTUI	ATCGAT	2 cut(s)	1887, 4480
BtgI	CCRYGG	2 cut(s)	860, 2599
BtsI	GCAGTG	2 cut(s)	1210, 3605
BveI	ACCTGC	2 cut(s)	2491, 3983
Cac8I	GCNNGC	7 cut(s)	1703, 2333, 2384, 2532, 2668, 3759, 4038
CaiI	CAGNNNCTG	3 cut(s)	1156, 1334, 1418
CciI	TCATGA	2 cut(s)	1750, 3802
CfoI	GCGC	2 cut(s)	2907, 3467
Cfr10I	RCCGGY	1 cut(s)	81
Cfr9I	CCCGGG	1 cut(s)	4185
ClaI	ATCGAT	2 cut(s)	1887, 4480
CseI	GACGC	3 cut(s)	2010, 3542, 4094
CsiI	ACCWGGT	1 cut(s)	2397
CspCI	CAANNNNNGTGG	2 cut(s)	1916, 1951
DraI	TTTAAA	3 cut(s)	1696, 2017, 3579
DraIII	CACNNNGTG	1 cut(s)	1422
EaeI	YGGCCR	1 cut(s)	552
Eam1104I	CTCTTC	5 cut(s)	861, 2478, 3007, 3048, 4383
EarI	CTCTTC	5 cut(s)	861, 2478, 3007, 3048, 4383
Ecl136II	GAGCTC	2 cut(s)	1805, 4040
Eco130I	CCWWGG	4 cut(s)	418, 860, 1982, 2599
Eco147I	AGGCCT	1 cut(s)	1978
Eco24I	GRGCYC	5 cut(s)	270, 1807, 2625, 2959, 4042
Eco47I	GGWCC	5 cut(s)	1039, 1241, 2168, 4189, 4368
Eco53kI	GAGCTC	2 cut(s)	1805, 4040
Eco57I	CTGAAG	9 cut(s)	873, 1539, 1856, 2321, 2535, 2556, 3509, 3800, 3881
Eco72I	CACGTG	1 cut(s)	4566
Eco88I	CYCGRG	3 cut(s)	1800, 3027, 4185
Eco91I	GGTNACC	1 cut(s)	983
EcoICRI	GAGCTC	2 cut(s)	1805, 4040
EcoO109I	RGGNCCY	4 cut(s)	2168, 2621, 2622, 4368
EcoO65I	GGTNACC	1 cut(s)	983
EcoRI	GAATTC	2 cut(s)	404, 1754
EcoRII	CCWGG	7 cut(s)	619, 703, 1093, 2170, 2397, 3171, 3626
EcoT14I	CCWWGG	4 cut(s)	418, 860, 1982, 2599
EcoT22I	ATGCAT	2 cut(s)	748, 3858
EcoT38I	GRGCYC	5 cut(s)	270, 1807, 2625, 2959, 4042
ErhI	CCWWGG	4 cut(s)	418, 860, 1982, 2599
FalI	AAGNNNNNCTT	6 cut(s)	1966, 1998, 2952, 2984, 3474, 3506
FaqI	GGGAC	2 cut(s)	2020, 4202
FauI	CCCGC	2 cut(s)	2813, 3525
FauNDI	CATATG	2 cut(s)	2437, 4440
FbaI	TGATCA	3 cut(s)	1162, 3505, 3514
FblI	GTMKAC	6 cut(s)	45, 2024, 3225, 3982, 4175, 4218
FriOI	GRGCYC	5 cut(s)	270, 1807, 2625, 2959, 4042
FspBI	CTAG	9 cut(s)	1560, 1808, 2667, 2747, 2798, 2868, 3825, 4307, 4323
GlaI	GCGC	2 cut(s)	2906, 3466
GsaI	CCCAGC	2 cut(s)	992, 1448
GsuI	CTGGAG	4 cut(s)	1758, 2511, 3961, 4133
HaeII	RGCGCY	1 cut(s)	3468
HaeIII	GGCC	8 cut(s)	554, 703, 714, 778, 1603, 1978, 2623, 2921
HapII	CCGG	4 cut(s)	82, 914, 2612, 4186
HgaI	GACGC	3 cut(s)	2010, 3542, 4094
HhaI	GCGC	2 cut(s)	2907, 3467
Hin6I	GCGC	2 cut(s)	2905, 3465
HinP1I	GCGC	2 cut(s)	2905, 3465
HincII	GTYRAC	2 cut(s)	46, 3317
HindII	GTYRAC	2 cut(s)	46, 3317
HindIII	AAGCTT	1 cut(s)	4148
HpaII	CCGG	4 cut(s)	82, 914, 2612, 4186
Hpy99I	CGWCG	1 cut(s)	3558
HpyCH4IV	ACGT	5 cut(s)	846, 1320, 2230, 4459, 4565
HpySE526I	ACGT	5 cut(s)	846, 1320, 2230, 4459, 4565
HspAI	GCGC	2 cut(s)	2905, 3465
KpnI	GGTACC	1 cut(s)	2398
Ksp22I	TGATCA	3 cut(s)	1162, 3505, 3514
MabI	ACCWGGT	1 cut(s)	2397
MaeI	CTAG	9 cut(s)	1560, 1808, 2667, 2747, 2798, 2868, 3825, 4307, 4323
MaeII	ACGT	5 cut(s)	846, 1320, 2230, 4459, 4565
MaeIII	GTNAC	9 cut(s)	322, 904, 983, 1152, 1196, 1414, 1630, 2267, 3083
MflI	RGATCY	8 cut(s)	147, 1097, 1642, 2818, 3136, 3454, 3608, 3821
MhlI	GDGCHC	7 cut(s)	270, 333, 1410, 1807, 2625, 2959, 4042
MlsI	TGGCCA	1 cut(s)	554
MluNI	TGGCCA	1 cut(s)	554
MlyI	GAGTC	4 cut(s)	1757, 1805, 2002, 3732
MmeI	TCCRAC	6 cut(s)	1260, 1303, 1920, 1981, 2235, 4374
Mox20I	TGGCCA	1 cut(s)	554
Mph1103I	ATGCAT	2 cut(s)	748, 3858
MroXI	GAANNNNTTC	4 cut(s)	1552, 3114, 3370, 3810
MscI	TGGCCA	1 cut(s)	554
MslI	CAYNNNNRTG	7 cut(s)	690, 1047, 1173, 1194, 1527, 2421, 3174
Msp20I	TGGCCA	1 cut(s)	554
MspA1I	CMGCKG	2 cut(s)	473, 2297
MspCI	CTTAAG	2 cut(s)	3305, 3956
MspI	CCGG	4 cut(s)	82, 914, 2612, 4186
Mva1269I	GAATGC	2 cut(s)	1746, 4051
MvaI	CCWGG	7 cut(s)	621, 705, 1095, 2172, 2399, 3173, 3628
MvnI	CGCG	1 cut(s)	2907
NciI	CCSGG	4 cut(s)	914, 2612, 4186, 4187
NcoI	CCATGG	2 cut(s)	860, 2599
NdeI	CATATG	2 cut(s)	2437, 4440
NheI	GCTAGC	1 cut(s)	2666
NmuCI	GTSAC	5 cut(s)	322, 1196, 1414, 2267, 3083
NsiI	ATGCAT	2 cut(s)	748, 3858
NspI	RCATGY	7 cut(s)	168, 301, 571, 689, 878, 1781, 1901
OliI	CACNNNNGTG	1 cut(s)	3174
PaeR7I	CTCGAG	1 cut(s)	1800
PagI	TCATGA	2 cut(s)	1750, 3802
PaqCI	CACCTGC	2 cut(s)	2491, 3983
PceI	AGGCCT	1 cut(s)	1978
PciI	ACATGT	4 cut(s)	164, 685, 874, 1777
PctI	GAATGC	2 cut(s)	1746, 4051
PdmI	GAANNNNTTC	4 cut(s)	1552, 3114, 3370, 3810
PfeI	GAWTC	8 cut(s)	573, 727, 1592, 1950, 2198, 2689, 2837, 3806
PflFI	GACNNNGTC	1 cut(s)	502
PflMI	CCANNNNNTGG	2 cut(s)	866, 1652
PfoI	TCCNGGA	3 cut(s)	912, 1093, 2170
PleI	GAGTC	4 cut(s)	1757, 1804, 2002, 3731
PmaCI	CACGTG	1 cut(s)	4566
PmlI	CACGTG	1 cut(s)	4566
PpsI	GAGTC	4 cut(s)	1757, 1804, 2002, 3731
Ppu21I	YACGTR	3 cut(s)	2231, 4460, 4566
PpuMI	RGGWCCY	2 cut(s)	2168, 4368
PscI	ACATGT	4 cut(s)	164, 685, 874, 1777
PshBI	ATTAAT	4 cut(s)	27, 827, 3692, 3768
PsiI	TTATAA	1 cut(s)	1112
Psp124BI	GAGCTC	2 cut(s)	1807, 4042
Psp5II	RGGWCCY	2 cut(s)	2168, 4368
Psp6I	CCWGG	7 cut(s)	619, 703, 1093, 2170, 2397, 3171, 3626
PspCI	CACGTG	1 cut(s)	4566
PspEI	GGTNACC	1 cut(s)	983
PspFI	CCCAGC	2 cut(s)	988, 1444
PspGI	CCWGG	7 cut(s)	619, 703, 1093, 2170, 2397, 3171, 3626
PspOMI	GGGCCC	1 cut(s)	2621
PspPPI	RGGWCCY	2 cut(s)	2168, 4368
PstI	CTGCAG	2 cut(s)	1205, 2680
PstNI	CAGNNNCTG	3 cut(s)	1156, 1334, 1418
PsuI	RGATCY	8 cut(s)	147, 1097, 1642, 2818, 3136, 3454, 3608, 3821
PsyI	GACNNNGTC	1 cut(s)	502
PvuII	CAGCTG	2 cut(s)	473, 2297
RseI	CAYNNNNRTG	7 cut(s)	690, 1047, 1173, 1194, 1527, 2421, 3174
SacI	GAGCTC	2 cut(s)	1807, 4042
SalI	GTCGAC	1 cut(s)	44
ScaI	AGTACT	1 cut(s)	1370
SchI	GAGTC	4 cut(s)	1757, 1805, 2002, 3732
SduI	GDGCHC	7 cut(s)	270, 333, 1410, 1807, 2625, 2959, 4042
SexAI	ACCWGGT	1 cut(s)	2397
SfcI	CTRYAG	9 cut(s)	384, 1201, 1251, 1260, 1348, 1909, 1995, 2179, 2676
Sfr274I	CTCGAG	1 cut(s)	1800
SinI	GGWCC	5 cut(s)	1039, 1241, 2168, 4189, 4368
SlaI	CTCGAG	1 cut(s)	1800
SmaI	CCCGGG	1 cut(s)	4187
SmiMI	CAYNNNNRTG	7 cut(s)	690, 1047, 1173, 1194, 1527, 2421, 3174
SmlI	CTYRAG	6 cut(s)	1800, 2066, 2915, 3305, 3924, 3956
SmoI	CTYRAG	6 cut(s)	1800, 2066, 2915, 3305, 3924, 3956
SseBI	AGGCCT	1 cut(s)	1978
SsiI	CCGC	4 cut(s)	1216, 2806, 3532, 4226
SspMI	CTAG	9 cut(s)	1560, 1808, 2667, 2747, 2798, 2868, 3825, 4307, 4323
SstI	GAGCTC	2 cut(s)	1807, 4042
StuI	AGGCCT	1 cut(s)	1978
StyI	CCWWGG	4 cut(s)	418, 860, 1982, 2599
TaiI	ACGT	5 cut(s)	849, 1323, 2233, 4462, 4568
TaqI	TCGA	9 cut(s)	45, 1294, 1393, 1575, 1801, 1852, 1887, 3118, 4480
TatI	WGTACW	5 cut(s)	367, 1368, 1678, 4168, 4206
TauI	GCSGC	2 cut(s)	1219, 4228
TfiI	GAWTC	8 cut(s)	573, 727, 1592, 1950, 2198, 2689, 2837, 3806
TseFI	GTSAC	5 cut(s)	322, 1196, 1414, 2267, 3083
Tsp45I	GTSAC	5 cut(s)	322, 1196, 1414, 2267, 3083
TspGWI	ACGGA	3 cut(s)	125, 477, 2274
TspMI	CCCGGG	1 cut(s)	4185
Tth111I	GACNNNGTC	1 cut(s)	502
Van91I	CCANNNNNTGG	2 cut(s)	866, 1652
Vha464I	CTTAAG	2 cut(s)	3305, 3956
VpaK11BI	GGWCC	5 cut(s)	1039, 1241, 2168, 4189, 4368
VspI	ATTAAT	4 cut(s)	27, 827, 3692, 3768
XceI	RCATGY	7 cut(s)	168, 301, 571, 689, 878, 1781, 1901
XcmI	CCANNNNNNNNNTGG	3 cut(s)	347, 1419, 1460
XhoI	CTCGAG	1 cut(s)	1800
XmaI	CCCGGG	1 cut(s)	4185
XmiI	GTMKAC	6 cut(s)	45, 2024, 3225, 3982, 4175, 4218
XmnI	GAANNNNTTC	4 cut(s)	1552, 3114, 3370, 3810
XspI	CTAG	9 cut(s)	1560, 1808, 2667, 2747, 2798, 2868, 3825, 4307, 4323
ZrmI	AGTACT	1 cut(s)	1370
Zsp2I	ATGCAT	2 cut(s)	748, 3858

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.