pycom08g18570

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003069
Physical Location & Seq
Forward (+)
18708955 .. 18709942
988 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGAGCCTTGCCCGCCAAGGGAACTATTCAAGTTTGGGTCACCGGGTGTATGTGTCGGCAATTGACGTGAACGATGTTCCTCTGAATAAATATCTGCATGGAGTAACTTGTGAACAAAATATAATTTCTCTCCAGCATGCAGGGGCTAAGTGTGTGAATCGAGCTGATTTGAGAAATGGCCATTCTCAAGGTCTTATTCCGGATTCCTTTTCATTAATGCATGGTCGTGATGCTGAGAATGGCTACTCTGCTAGAGTTGCGGTTGATAAGAGTGATATGGTTCAGGGTCCTCTGCCACGCACATGTAGAGAGGAACTGAAAGCAGACGGTCTGGTTCTAGACAAGGGATTTTCAAATGAGAATTCTGGTTTAAGACCCCAAATAGATTTATATAGATGTTTAACTGAAGAAGAAACTGAAACGACTGCCGCTTCTCCAATTATGAGGACGGAAACTGTGATCGATTTGGAGGCACCGGTAGTAGTTGAAACAGATATTTTTGAGGAAGATTCCATTGAAAGAAGAGGTAAGGACTCACCTCATGAGGGCCTTATCAGAGTAGCAGCTGAGGCTCTAGTTGCCATCTCATCGTCTCAAACTCCCAATATGCAGGACAATGCCACTGGTCACAAGATGGATTCTTCGCAAAACGACTCCCTTCTTTGGTTTGCAAAAACAATTTCTTCATGTGATGGAAATCTTGACAATGGAAGTGCGGCAGAGGTTAAGCGAACTGACAGTGAAGAGTCATTTCCTGATGTGACGAATTACTTTGAGCACATGACATTAAACTTGGTGGAAACCAAGGAAGAGTGGCCCTGCTATGTGCCTCCAAATTCAGACAACCTAATAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

286

Amino Acids

31.43

Weight (kDa)

4.66

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 104 - 271 3.4e-28 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 472
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 4 cut(s) 13, 260, 429, 716
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 2 cut(s) 361, 835
AcuI CTGAAG 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 46
AfiI CCNNNNNNNGG 3 cut(s) 18, 545, 663
AflIII ACRYGT 1 cut(s) 302
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 4 cut(s) 30, 354, 488, 518
AjiI CACGTC 1 cut(s) 67
AluBI AGCT 2 cut(s) 164, 566
AluI AGCT 2 cut(s) 164, 566
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 597
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 3 cut(s) 178, 547, 815
ApeKI GCWGC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 361, 835
AseI ATTAAT 1 cut(s) 215
AsiGI ACCGGT 1 cut(s) 475
AspS9I GGNCC 3 cut(s) 287, 547, 816
AsuC2I CCSGG 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 32, 528
AvaII GGWCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 180
BanI GGYRCC 1 cut(s) 472
Bbv12I GWGCWC 1 cut(s) 780
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 575
BccI CCATC 3 cut(s) 590, 628, 686
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 3 cut(s) 252, 338, 575
BisI GCNGC 3 cut(s) 429, 564, 717
BlsI GCNGC 3 cut(s) 430, 565, 718
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 3 cut(s) 287, 547, 816
BmiI GGNNCC 2 cut(s) 288, 474
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 16, 804
BsaWI WCCGGW 2 cut(s) 199, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 545, 663
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 199
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 16, 804
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 3 cut(s) 18, 545, 663
BseMII CTCAG 2 cut(s) 225, 558
BseNI ACTGG 1 cut(s) 628
BseXI GCAGC 1 cut(s) 575
BshFI GGCC 3 cut(s) 180, 549, 817
BshNI GGYRCC 1 cut(s) 472
BshTI ACCGGT 1 cut(s) 475
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 3 cut(s) 43, 200, 476
BslI CCNNNNNNNGG 3 cut(s) 18, 545, 663
BsmAI GTCTC 1 cut(s) 597
BsmBI CGTCTC 1 cut(s) 597
BsnI GGCC 3 cut(s) 180, 549, 817
Bsp1286I GDGCHC 1 cut(s) 780
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 1 cut(s) 459
BspACI CCGC 4 cut(s) 13, 260, 429, 716
BspANI GGCC 3 cut(s) 180, 549, 817
BspCNI CTCAG 2 cut(s) 226, 559
BspDI ATCGAT 1 cut(s) 462
BspEI TCCGGA 1 cut(s) 199
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 288, 474
BspT107I GGYRCC 1 cut(s) 472
BsrFI RCCGGY 1 cut(s) 475
BsrI ACTGG 1 cut(s) 628
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 16, 804
BssMI GATC 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 16, 804
Bst4CI ACNGT 3 cut(s) 329, 457, 740
Bst6I CTCTTC 3 cut(s) 517, 738, 804
BstC8I GCNNGC 2 cut(s) 13, 138
BstDEI CTNAG 3 cut(s) 147, 234, 567
BstEII GGTNACC 1 cut(s) 38
BstKTI GATC 1 cut(s) 462
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 1 cut(s) 459
BstMWI GCNNNNNNNGC 4 cut(s) 12, 257, 569, 578
BstNSI RCATGY 2 cut(s) 140, 306
BstPI GGTNACC 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 42
BstV1I GCAGC 1 cut(s) 575
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 3 cut(s) 180, 549, 817
BsuTUI ATCGAT 1 cut(s) 462
BtrI CACGTC 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 621, 745
Cac8I GCNNGC 2 cut(s) 13, 138
CciI TCATGA 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 3 cut(s) 287, 547, 816
ClaI ATCGAT 1 cut(s) 462
CspAI ACCGGT 1 cut(s) 475
CviAII CATG 7 cut(s) 98, 137, 221, 303, 542, 687, 781
CviJI RGCY 9 cut(s) 6, 146, 164, 180, 243, 549, 566, 572, 817
CviKI_1 RGCY 9 cut(s) 6, 146, 164, 180, 243, 549, 566, 572, 817
DdeI CTNAG 3 cut(s) 147, 234, 567
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
DraIII CACNNNGTG 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 517, 738, 804
EarI CTCTTC 3 cut(s) 517, 738, 804
Eco130I CCWWGG 2 cut(s) 16, 804
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 426
Eco91I GGTNACC 1 cut(s) 38
EcoO109I RGGNCCY 2 cut(s) 287, 547
EcoO65I GGTNACC 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 16, 804
EcoT22I ATGCAT 1 cut(s) 222
ErhI CCWWGG 2 cut(s) 16, 804
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 7 cut(s) 101, 140, 224, 306, 545, 690, 784
FatI CATG 7 cut(s) 97, 136, 220, 302, 541, 686, 780
FauI CCCGC 1 cut(s) 20
Fnu4HI GCNGC 3 cut(s) 429, 564, 717
Fsp4HI GCNGC 3 cut(s) 429, 564, 717
FspBI CTAG 3 cut(s) 252, 338, 575
GluI GCNGC 3 cut(s) 429, 564, 717
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 3 cut(s) 180, 549, 817
HapII CCGG 3 cut(s) 43, 200, 476
Hin1II CATG 7 cut(s) 101, 140, 224, 306, 545, 690, 784
HinfI GANTC 7 cut(s) 157, 203, 509, 533, 638, 653, 746
HpaII CCGG 3 cut(s) 43, 200, 476
HphI GGTGA 2 cut(s) 32, 528
Hpy166II GTNNAC 2 cut(s) 70, 113
Hpy188I TCNGA 3 cut(s) 84, 557, 841
Hpy188III TCNNGA 6 cut(s) 200, 227, 338, 542, 701, 755
Hpy8I GTNNAC 2 cut(s) 70, 113
HpyAV CCTTC 1 cut(s) 668
HpyCH4III ACNGT 3 cut(s) 329, 457, 740
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 5 cut(s) 97, 140, 220, 610, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 257, 569, 578
HpyF3I CTNAG 3 cut(s) 147, 234, 567
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 7 cut(s) 101, 140, 224, 306, 545, 690, 784
Kpn2I TCCGGA 1 cut(s) 199
Kzo9I GATC 1 cut(s) 459
Lsp1109I GCAGC 1 cut(s) 575
LweI GCATC 1 cut(s) 220
MaeI CTAG 3 cut(s) 252, 338, 575
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 4 cut(s) 38, 103, 626, 760
MalI GATC 1 cut(s) 461
MboI GATC 1 cut(s) 459
MboII GAAGA 8 cut(s) 419, 422, 518, 534, 633, 675, 755, 821
MfeI CAATTG 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 7 cut(s) 60, 123, 361, 438, 678, 766, 835
MluNI TGGCCA 1 cut(s) 180
MlyI GAGTC 3 cut(s) 527, 647, 755
Mox20I TGGCCA 1 cut(s) 180
Mph1103I ATGCAT 1 cut(s) 222
MroI TCCGGA 1 cut(s) 199
MscI TGGCCA 1 cut(s) 180
MseI TTAA 5 cut(s) 215, 371, 401, 726, 788
MslI CAYNNNNRTG 2 cut(s) 225, 301
Msp20I TGGCCA 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 3 cut(s) 43, 200, 476
MspR9I CCNGG 1 cut(s) 44
MunI CAATTG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 12, 257, 569, 578
NciI CCSGG 1 cut(s) 44
NdeII GATC 1 cut(s) 459
NlaIII CATG 7 cut(s) 101, 140, 224, 306, 545, 690, 784
NlaIV GGNNCC 2 cut(s) 288, 474
NmuCI GTSAC 3 cut(s) 38, 626, 760
NsiI ATGCAT 1 cut(s) 222
NspI RCATGY 2 cut(s) 140, 306
PaeI GCATGC 1 cut(s) 140
PagI TCATGA 1 cut(s) 541
PciI ACATGT 1 cut(s) 302
PfeI GAWTC 4 cut(s) 157, 203, 509, 638
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 3 cut(s) 430, 565, 718
PleI GAGTC 3 cut(s) 527, 647, 754
PpsI GAGTC 3 cut(s) 527, 647, 754
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 302
PshBI ATTAAT 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 287
PspEI GGTNACC 1 cut(s) 38
PspN4I GGNNCC 2 cut(s) 288, 474
PspPI GGNCC 3 cut(s) 287, 547, 816
PspPPI RGGWCCY 1 cut(s) 287
PvuII CAGCTG 1 cut(s) 566
RseI CAYNNNNRTG 2 cut(s) 225, 301
SaqAI TTAA 5 cut(s) 215, 371, 401, 726, 788
SatI GCNGC 3 cut(s) 429, 564, 717
Sau3AI GATC 1 cut(s) 459
Sau96I GGNCC 3 cut(s) 287, 547, 816
SchI GAGTC 3 cut(s) 527, 647, 755
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 780
SetI ASST 8 cut(s) 69, 166, 193, 529, 541, 568, 726, 849
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 2 cut(s) 225, 301
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
SphI GCATGC 1 cut(s) 140
Sse9I AATT 7 cut(s) 60, 123, 361, 438, 678, 766, 835
SsiI CCGC 4 cut(s) 13, 260, 429, 716
SspMI CTAG 3 cut(s) 252, 338, 575
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 2 cut(s) 16, 804
TaaI ACNGT 3 cut(s) 329, 457, 740
TaiI ACGT 1 cut(s) 69
TaqI TCGA 2 cut(s) 160, 462
TasI AATT 7 cut(s) 60, 123, 361, 438, 678, 766, 835
TauI GCSGC 2 cut(s) 431, 719
TfiI GAWTC 4 cut(s) 157, 203, 509, 638
Tru1I TTAA 5 cut(s) 215, 371, 401, 726, 788
Tru9I TTAA 5 cut(s) 215, 371, 401, 726, 788
TscAI CASTG 2 cut(s) 628, 745
TseFI GTSAC 3 cut(s) 38, 626, 760
TseI GCWGC 1 cut(s) 563
Tsp45I GTSAC 3 cut(s) 38, 626, 760
TspDTI ATGAA 2 cut(s) 201, 675
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 2 cut(s) 628, 745
VpaK11BI GGWCC 1 cut(s) 287
VspI ATTAAT 1 cut(s) 215
XapI RAATTY 2 cut(s) 361, 835
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 2 cut(s) 140, 306
XspI CTAG 3 cut(s) 252, 338, 575
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.