Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0003345

Physical Location & Seq

Genomic Coordinates Reverse (-)

887418 .. 891378

Gene Structure 3961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGTCGATTCGAGTGCTGAATCCGAATGCGGAGGTGCTGAACAAATCGGCGGCGCTACACATGAACATCAACGCCGCCAAAGGCTTGCAGGACGTCCTCAAGACCAACCTCGGCCCCAAGGGCACCATCAAAATGCTTGTTGGCGGAGCCGGCGACATTAAGCTCACTAAGGATGGTAACACTCTGCTCAAAGAAATGCAAATTCAAAACCCAACGGCAATTATGATTGCGCGTACAGCTGTTGCCCAAGATGACATTAGCGGAGATGGCACCACGTCTACTGTCTTGTTCATTGGCGAGCTTATGAAACAATCAGAACGATACATTGACGAAGGGATGCATCCCCGTGTCCTGGTTGATGGTTTTGAGATAGCCAAAAGAGCAATGCTGCAGTTTCTTGAGAAATTCAAAACTCCTGTGGTGATGGGCAATGAGCCTGACAAAGAGGTTTTGAAAATGGTAGCAAGAACAACATTGCGAACAAAGTTATATGAAGCATTGGCAGATCAATTGACTGACATAGTTGTTAATTCGGTTCTTTGCATCCGCAAACCTGAGGAGCCCATTGATCTGTTCATGGTGGAGATTATGCACATGCGACACAAATTTGATGTAGACACACGCTTGGTTGAGGGTCTTGTTCTCGATCACGGTTCTAGGCATCCTGATATGAAGCGGAGAGCAGAGAATTGTTTCATTTTGACAAGCAATGTATCTTTGGAATATGACAAAAGTGAAGTAAACTCAGGCTTTTTCTATTCAAATGCGGAGCAGAGAGAAGCAATGGTTTTAGCTGAAAGGCGCCAGGTGTGCTCTGGTAATGATAATAACTTTGTCGTTATCAATCAGAAGGGAATTGATCGATCCCCATCTCTGGACCTTCTCGCAAGGGCAGGGATTATTGCCCTGAGAAGAGCAAAGAGGAGAAATATGGAACGGTTGGTTTTGGCTTGTGGCGGGGAGGCTGTAAACTCCGTAGATGATTTAACTCCTGATTGCCTTGGTTGGGCTGGACTTGTATACGAGCATGTCCTTGGTGAAGAGAAGTATACATTTGTTGAAAATGTGAAGAATCCCCACTCTTGCACAATCTTAATCAAAGGGCCTAATGACCATACAATTGCTCAGATTAAGGATGCTGTTCGTGATGGCCTGAGGGCAGTTAAAAACACAGTTGAAGATGAAGCTGTCATCTTAGGTGCTGGAGCTTTTGAAGTTGCAGCCAGACAATATTTAGTGAATGAAGTAAAGAAAACTGTTAAAGGGCGTGCTCAACTCGGTGTTGAAGCTTTTGCTGATGCTCTTCTTGTGGTGCCCAAAACACTTGCTGAGAATTCTGGCCTCGATACACAAGATGTGATAATTGCTCTTACGGGAGAGCATGATCGGGGAAATGTCGTGGGACTAAATCACAACACTGGAGAACCGCTTGATCCACAGATGGAGGGTATCTTTGACAACTACTCTGTGAAGCGACAGATTATAAACTCAGGGCCCGTAATTGCATCTCAGCTGTTGTTGGTCGATGAAGTAATTCGTGCTGGGCGTAACATGCGGAAGCCAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005832 GO:0006457 GO:0006458 GO:0008150 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0032991 GO:0042221 GO:0044183 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046686 GO:0050896 GO:0051082 GO:0061077 GO:0101031

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

523

Amino Acids

57.58

Weight (kDa)

6.07

Isoelectric Point (pI)

31.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	29 - 515	4.4e-157	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003345)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02530 AT5G16070
fragaria_vesca	FvH4_1g11210
malus_domestica	MD09G1086000.v1.1 MD17G1104900.v1.1
prunus_persica	Prupe.3G238800_v2.0.a1
pyrus_communis	pycom09g01130 pycom17g07580
rosa_chinensis	RchiOBHm_Chr2g0098621 RchiOBHm_Chr2g0138531 RchiOBHm_Chr4g0407281 RchiOBHm_Chr4g0409191 RchiOBHm_Chr6g0270681
rosa_laevigata	RLG00000013776 RLG00000016815
rosa_multiflora	Rmu_co8186612.1_g000001 Rmu_sc0000474.1_g000003 Rmu_sc0002106.1_g000012 Rmu_sc0002776.1_g000012
rosa_roxburghii	Rroxscaffold_2G00143840 Rroxscaffold_7G00197690
rosa_rugosa	Rorug02G0073800 Rorug06G0055700
rosa_samantha	Rh2AG121400 Rh2BG125000 Rh2CG126000 Rh2DG127100 Rh5BG537700 Rh6AG175000 Rh6AG275700 Rh6CG174300 Rh6DG166700 Rh7CG256600
rosa_wichuraiana	Rw0G004140 Rw0G012590 Rw6G014910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1484
AatII	GACGTC	1 cut(s)	96
AccB1I	GGYRCC	4 cut(s)	122, 269, 801, 1312
AccI	GTMKAC	4 cut(s)	278, 615, 1020, 1049
AccII	CGCG	1 cut(s)	232
AclWI	GGATC	2 cut(s)	858, 1427
AcsI	RAATTY	4 cut(s)	201, 404, 605, 1333
AcyI	GRCGYC	2 cut(s)	93, 802
AfaI	GTAC	1 cut(s)	235
AfiI	CCNNNNNNNGG	3 cut(s)	352, 874, 1006
AgsI	TTSAA	8 cut(s)	206, 409, 454, 762, 1061, 1178, 1214, 1286
AjiI	CACGTC	1 cut(s)	276
AjnI	CCWGG	2 cut(s)	351, 804
AluBI	AGCT	8 cut(s)	163, 239, 301, 794, 1187, 1208, 1289, 1513
AluI	AGCT	8 cut(s)	163, 239, 301, 794, 1187, 1208, 1289, 1513
Alw21I	GWGCWC	2 cut(s)	815, 1273
AlwI	GGATC	2 cut(s)	858, 1427
AoxI	GGCC	5 cut(s)	112, 1103, 1150, 1339, 1493
ApaI	GGGCCC	1 cut(s)	1497
ApeKI	GCWGC	2 cut(s)	388, 1220
ApoI	RAATTY	4 cut(s)	201, 404, 605, 1333
Asp700I	GAANNNNTTC	2 cut(s)	692, 1533
AspLEI	GCGC	3 cut(s)	55, 232, 804
AspS9I	GGNCC	5 cut(s)	113, 877, 1103, 1493, 1494
AsuHPI	GGTGA	2 cut(s)	433, 1049
AvaII	GGWCC	1 cut(s)	877
AxyI	CCTNAGG	2 cut(s)	555, 1154
BaeGI	GKGCMC	3 cut(s)	125, 1317, 1497
BanI	GGYRCC	4 cut(s)	122, 269, 801, 1312
BanII	GRGCYC	2 cut(s)	564, 1497
Bbv12I	GWGCWC	2 cut(s)	815, 1273
BbvI	GCAGC	2 cut(s)	375, 1232
BccI	CCATC	8 cut(s)	134, 167, 260, 353, 418, 877, 1142, 1435
BceAI	ACGGC	1 cut(s)	231
BcgI	CGANNNNNNTGC	1 cut(s)	29
BciT130I	CCWGG	2 cut(s)	353, 806
BfaI	CTAG	1 cut(s)	657
BfmI	CTRYAG	1 cut(s)	389
BfoI	RGCGCY	2 cut(s)	56, 805
BglI	GCCNNNNNGGC	1 cut(s)	120
BisI	GCNGC	4 cut(s)	51, 75, 389, 1221
BlsI	GCNGC	4 cut(s)	52, 76, 390, 1222
Bme1390I	CCNGG	2 cut(s)	353, 806
Bme18I	GGWCC	1 cut(s)	877
BmgBI	CACGTC	1 cut(s)	276
BmgT120I	GGNCC	5 cut(s)	113, 877, 1103, 1493, 1494
BmiI	GGNNCC	8 cut(s)	115, 124, 148, 271, 561, 803, 1314, 1495
BmrFI	CCNGG	2 cut(s)	353, 806
BmsI	GCATC	7 cut(s)	327, 349, 552, 670, 1126, 1288, 1514
BpmI	CTGGAG	2 cut(s)	1224, 1440
BpuEI	CTTGAG	2 cut(s)	83, 419
Bsa29I	ATCGAT	1 cut(s)	862
BsaBI	GATNNNNATC	1 cut(s)	868
BsaHI	GRCGYC	2 cut(s)	93, 802
BsaJI	CCNNGG	4 cut(s)	109, 117, 1000, 1033
Bsc4I	CCNNNNNNNGG	3 cut(s)	352, 874, 1006
Bse118I	RCCGGY	1 cut(s)	149
Bse1I	ACTGG	1 cut(s)	1423
Bse21I	CCTNAGG	2 cut(s)	555, 1154
Bse3DI	GCAATG	5 cut(s)	390, 436, 473, 715, 789
Bse8I	GATNNNNATC	1 cut(s)	868
BseBI	CCWGG	2 cut(s)	353, 806
BseCI	ATCGAT	1 cut(s)	862
BseDI	CCNNGG	4 cut(s)	109, 117, 1000, 1033
BseGI	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1141
BseJI	GATNNNNATC	1 cut(s)	868
BseLI	CCNNNNNNNGG	3 cut(s)	352, 874, 1006
BseMI	GCAATG	5 cut(s)	390, 436, 473, 715, 789
BseMII	CTCAG	8 cut(s)	546, 759, 899, 1139, 1145, 1320, 1503, 1523
BseNI	ACTGG	1 cut(s)	1423
BseRI	GAGGAG	2 cut(s)	572, 937
BseSI	GKGCMC	3 cut(s)	125, 1317, 1497
BseXI	GCAGC	2 cut(s)	375, 1232
BseYI	CCCAGC	1 cut(s)	1541
Bsh1236I	CGCG	1 cut(s)	232
BshFI	GGCC	5 cut(s)	114, 1105, 1152, 1341, 1495
BshNI	GGYRCC	4 cut(s)	122, 269, 801, 1312
BshVI	ATCGAT	1 cut(s)	862
BsiHKAI	GWGCWC	2 cut(s)	815, 1273
BsiSI	CCGG	1 cut(s)	150
BslFI	GGGAC	1 cut(s)	1416
BslI	CCNNNNNNNGG	3 cut(s)	352, 874, 1006
BsmFI	GGGAC	1 cut(s)	1416
BsmI	GAATGC	1 cut(s)	31
BsnI	GGCC	5 cut(s)	114, 1105, 1152, 1341, 1495
Bsp120I	GGGCCC	1 cut(s)	1493
Bsp1286I	GDGCHC	6 cut(s)	125, 564, 815, 1273, 1317, 1497
Bsp143I	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
BspANI	GGCC	5 cut(s)	114, 1105, 1152, 1341, 1495
BspCNI	CTCAG	8 cut(s)	547, 758, 900, 1138, 1146, 1321, 1502, 1522
BspDI	ATCGAT	1 cut(s)	862
BspFNI	CGCG	1 cut(s)	232
BspLI	GGNNCC	8 cut(s)	115, 124, 148, 271, 561, 803, 1314, 1495
BspMAI	CTGCAG	1 cut(s)	393
BspPI	GGATC	2 cut(s)	858, 1427
BspQI	GCTCTTC	2 cut(s)	907, 1308
BspT107I	GGYRCC	4 cut(s)	122, 269, 801, 1312
BsrDI	GCAATG	5 cut(s)	390, 436, 473, 715, 789
BsrFI	RCCGGY	1 cut(s)	149
BsrI	ACTGG	1 cut(s)	1423
BssAI	RCCGGY	1 cut(s)	149
BssECI	CCNNGG	4 cut(s)	109, 117, 1000, 1033
BssMI	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
BssNAI	GTATAC	2 cut(s)	1021, 1050
BssNI	GRCGYC	2 cut(s)	93, 802
BssT1I	CCWWGG	3 cut(s)	117, 1000, 1033
Bst1107I	GTATAC	2 cut(s)	1021, 1050
Bst2UI	CCWGG	2 cut(s)	353, 806
Bst4CI	ACNGT	5 cut(s)	283, 653, 939, 1174, 1258
Bst6I	CTCTTC	3 cut(s)	907, 1035, 1308
BstACI	GRCGYC	2 cut(s)	93, 802
BstC8I	GCNNGC	4 cut(s)	86, 151, 299, 1269
BstF5I	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1141
BstFNI	CGCG	1 cut(s)	232
BstH2I	RGCGCY	2 cut(s)	56, 805
BstHHI	GCGC	3 cut(s)	55, 232, 804
BstKTI	GATC	7 cut(s)	508, 571, 649, 862, 866, 1387, 1435
BstMBI	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
BstMWI	GCNNNNNNNGC	7 cut(s)	120, 150, 236, 267, 380, 810, 1552
BstNI	CCWGG	2 cut(s)	353, 806
BstNSI	RCATGY	3 cut(s)	598, 1031, 1555
BstSCI	CCNGG	2 cut(s)	351, 804
BstSFI	CTRYAG	1 cut(s)	389
BstSLI	GKGCMC	3 cut(s)	125, 1317, 1497
BstUI	CGCG	1 cut(s)	232
BstV1I	GCAGC	2 cut(s)	375, 1232
BstZ17I	GTATAC	2 cut(s)	1021, 1050
Bsu15I	ATCGAT	1 cut(s)	862
Bsu36I	CCTNAGG	2 cut(s)	555, 1154
BsuRI	GGCC	5 cut(s)	114, 1105, 1152, 1341, 1495
BsuTUI	ATCGAT	1 cut(s)	862
BtrI	CACGTC	1 cut(s)	276
BtsCI	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1141
BtsIMutI	CAGTG	1 cut(s)	1416
Cac8I	GCNNGC	4 cut(s)	86, 151, 299, 1269
CfoI	GCGC	3 cut(s)	55, 232, 804
Cfr10I	RCCGGY	1 cut(s)	149
Cfr13I	GGNCC	5 cut(s)	113, 877, 1103, 1493, 1494
ClaI	ATCGAT	1 cut(s)	862
Csp6I	GTAC	1 cut(s)	234
CviAII	CATG	6 cut(s)	61, 577, 595, 1028, 1382, 1552
CviQI	GTAC	1 cut(s)	234
DinI	GGCGCC	1 cut(s)	803
DpnI	GATC	7 cut(s)	507, 570, 648, 861, 865, 1386, 1434
DpnII	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
Eam1104I	CTCTTC	3 cut(s)	907, 1035, 1308
EarI	CTCTTC	3 cut(s)	907, 1035, 1308
EciI	GGCGGA	1 cut(s)	159
Eco130I	CCWWGG	3 cut(s)	117, 1000, 1033
Eco24I	GRGCYC	2 cut(s)	564, 1497
Eco47I	GGWCC	1 cut(s)	877
Eco81I	CCTNAGG	2 cut(s)	555, 1154
EcoO109I	RGGNCCY	2 cut(s)	1103, 1493
EcoRI	GAATTC	1 cut(s)	1333
EcoRII	CCWGG	2 cut(s)	351, 804
EcoT14I	CCWWGG	3 cut(s)	117, 1000, 1033
EcoT22I	ATGCAT	1 cut(s)	342
EcoT38I	GRGCYC	2 cut(s)	564, 1497
EgeI	GGCGCC	1 cut(s)	803
EheI	GGCGCC	1 cut(s)	803
ErhI	CCWWGG	3 cut(s)	117, 1000, 1033
FaeI	CATG	6 cut(s)	64, 580, 598, 1031, 1385, 1555
FaqI	GGGAC	1 cut(s)	1416
FatI	CATG	6 cut(s)	60, 576, 594, 1027, 1381, 1551
FauI	CCCGC	1 cut(s)	950
FblI	GTMKAC	4 cut(s)	278, 615, 1020, 1049
Fnu4HI	GCNGC	4 cut(s)	51, 75, 389, 1221
FokI	GGATG	6 cut(s)	185, 327, 349, 530, 648, 1148
FriOI	GRGCYC	2 cut(s)	564, 1497
Fsp4HI	GCNGC	4 cut(s)	51, 75, 389, 1221
FspBI	CTAG	1 cut(s)	657
GlaI	GCGC	3 cut(s)	54, 231, 803
GluI	GCNGC	4 cut(s)	51, 75, 389, 1221
GsaI	CCCAGC	1 cut(s)	1545
GsuI	CTGGAG	2 cut(s)	1224, 1440
HaeII	RGCGCY	2 cut(s)	56, 805
HaeIII	GGCC	5 cut(s)	114, 1105, 1152, 1341, 1495
HapII	CCGG	1 cut(s)	150
HhaI	GCGC	3 cut(s)	55, 232, 804
Hin1I	GRCGYC	2 cut(s)	93, 802
Hin1II	CATG	6 cut(s)	64, 580, 598, 1031, 1385, 1555
Hin6I	GCGC	3 cut(s)	53, 230, 802
HinP1I	GCGC	3 cut(s)	53, 230, 802
HindIII	AAGCTT	1 cut(s)	1287
HinfI	GANTC	3 cut(s)	7, 19, 1072
HpaII	CCGG	1 cut(s)	150
HphI	GGTGA	2 cut(s)	433, 1049
Hpy166II	GTNNAC	6 cut(s)	279, 616, 742, 970, 1021, 1050
Hpy188I	TCNGA	4 cut(s)	24, 316, 849, 1128
Hpy188III	TCNNGA	7 cut(s)	100, 398, 644, 665, 875, 992, 1145
Hpy8I	GTNNAC	6 cut(s)	279, 616, 742, 970, 1021, 1050
HpyAV	CCTTC	3 cut(s)	326, 844, 890
HpyCH4III	ACNGT	5 cut(s)	283, 653, 939, 1174, 1258
HpyCH4IV	ACGT	2 cut(s)	93, 275
HpyCH4V	TGCA	9 cut(s)	88, 199, 340, 391, 543, 592, 1086, 1220, 1505
HpyF10VI	GCNNNNNNNGC	7 cut(s)	120, 150, 236, 267, 380, 810, 1552
HpySE526I	ACGT	2 cut(s)	93, 275
Hsp92I	GRCGYC	2 cut(s)	93, 802
Hsp92II	CATG	6 cut(s)	64, 580, 598, 1031, 1385, 1555
HspAI	GCGC	3 cut(s)	53, 230, 802
KasI	GGCGCC	1 cut(s)	801
KroI	GCCGGC	1 cut(s)	149
KroNI	GCCGGC	1 cut(s)	151
Kzo9I	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
LguI	GCTCTTC	2 cut(s)	907, 1308
LmnI	GCTCC	4 cut(s)	146, 559, 769, 1205
Lsp1109I	GCAGC	2 cut(s)	375, 1232
LweI	GCATC	7 cut(s)	327, 349, 552, 670, 1126, 1288, 1514
MaeI	CTAG	1 cut(s)	657
MaeII	ACGT	2 cut(s)	93, 275
MaeIII	GTNAC	2 cut(s)	176, 1547
MalI	GATC	7 cut(s)	507, 570, 648, 861, 865, 1386, 1434
MboI	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
MboII	GAAGA	5 cut(s)	924, 1052, 1081, 1190, 1295
MfeI	CAATTG	2 cut(s)	509, 1119
MhlI	GDGCHC	6 cut(s)	125, 564, 815, 1273, 1317, 1497
Mly113I	GGCGCC	1 cut(s)	802
Mph1103I	ATGCAT	1 cut(s)	342
MroNI	GCCGGC	1 cut(s)	149
MroXI	GAANNNNTTC	2 cut(s)	692, 1533
MseI	TTAA	8 cut(s)	159, 528, 986, 1094, 1131, 1164, 1260, 1567
MslI	CAYNNNNRTG	2 cut(s)	131, 345
MspA1I	CMGCKG	2 cut(s)	239, 1513
MspI	CCGG	1 cut(s)	150
MspR9I	CCNGG	2 cut(s)	353, 806
MunI	CAATTG	2 cut(s)	509, 1119
Mva1269I	GAATGC	1 cut(s)	31
MvaI	CCWGG	2 cut(s)	353, 806
MvnI	CGCG	1 cut(s)	232
MwoI	GCNNNNNNNGC	7 cut(s)	120, 150, 236, 267, 380, 810, 1552
NaeI	GCCGGC	1 cut(s)	151
NarI	GGCGCC	1 cut(s)	802
NdeII	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
NgoMIV	GCCGGC	1 cut(s)	149
NlaIII	CATG	6 cut(s)	64, 580, 598, 1031, 1385, 1555
NlaIV	GGNNCC	8 cut(s)	115, 124, 148, 271, 561, 803, 1314, 1495
NmeAIII	GCCGAG	1 cut(s)	90
NsiI	ATGCAT	1 cut(s)	342
NspI	RCATGY	3 cut(s)	598, 1031, 1555
PciSI	GCTCTTC	2 cut(s)	907, 1308
PcsI	WCGNNNNNNNCGW	1 cut(s)	1543
PctI	GAATGC	1 cut(s)	31
PdiI	GCCGGC	1 cut(s)	151
PdmI	GAANNNNTTC	2 cut(s)	692, 1533
PfeI	GAWTC	3 cut(s)	7, 19, 1072
PkrI	GCNGC	4 cut(s)	52, 76, 390, 1222
PluTI	GGCGCC	1 cut(s)	805
PsiI	TTATAA	1 cut(s)	1484
Psp6I	CCWGG	2 cut(s)	351, 804
PspFI	CCCAGC	1 cut(s)	1541
PspGI	CCWGG	2 cut(s)	351, 804
PspN4I	GGNNCC	8 cut(s)	115, 124, 148, 271, 561, 803, 1314, 1495
PspOMI	GGGCCC	1 cut(s)	1493
PspPI	GGNCC	5 cut(s)	113, 877, 1103, 1493, 1494
PstI	CTGCAG	1 cut(s)	393
PvuII	CAGCTG	2 cut(s)	239, 1513
RsaI	GTAC	1 cut(s)	235
RsaNI	GTAC	1 cut(s)	234
RseI	CAYNNNNRTG	2 cut(s)	131, 345
SapI	GCTCTTC	2 cut(s)	907, 1308
SaqAI	TTAA	8 cut(s)	159, 528, 986, 1094, 1131, 1164, 1260, 1567
SatI	GCNGC	4 cut(s)	51, 75, 389, 1221
Sau3AI	GATC	7 cut(s)	505, 568, 646, 859, 863, 1384, 1432
Sau96I	GGNCC	5 cut(s)	113, 877, 1103, 1493, 1494
ScrFI	CCNGG	2 cut(s)	353, 806
SduI	GDGCHC	6 cut(s)	125, 564, 815, 1273, 1317, 1497
SfaNI	GCATC	7 cut(s)	327, 349, 552, 670, 1126, 1288, 1514
SfcI	CTRYAG	1 cut(s)	389
SfoI	GGCGCC	1 cut(s)	803
SinI	GGWCC	1 cut(s)	877
SmiMI	CAYNNNNRTG	2 cut(s)	131, 345
SmlI	CTYRAG	2 cut(s)	98, 398
SmoI	CTYRAG	2 cut(s)	98, 398
SspDI	GGCGCC	1 cut(s)	801
SspI	AATATT	1 cut(s)	1232
SspMI	CTAG	1 cut(s)	657
StyD4I	CCNGG	2 cut(s)	351, 804
StyI	CCWWGG	3 cut(s)	117, 1000, 1033
TaaI	ACNGT	5 cut(s)	283, 653, 939, 1174, 1258
TaiI	ACGT	2 cut(s)	96, 278
TaqI	TCGA	6 cut(s)	5, 10, 645, 862, 1344, 1524
TauI	GCSGC	2 cut(s)	53, 77
TfiI	GAWTC	3 cut(s)	7, 19, 1072
Tru1I	TTAA	8 cut(s)	159, 528, 986, 1094, 1131, 1164, 1260, 1567
Tru9I	TTAA	8 cut(s)	159, 528, 986, 1094, 1131, 1164, 1260, 1567
TscAI	CASTG	1 cut(s)	1423
TseI	GCWGC	2 cut(s)	388, 1220
TspGWI	ACGGA	1 cut(s)	964
TspRI	CASTG	1 cut(s)	1423
VpaK11BI	GGWCC	1 cut(s)	877
XapI	RAATTY	4 cut(s)	201, 404, 605, 1333
XceI	RCATGY	3 cut(s)	598, 1031, 1555
XcmI	CCANNNNNNNNNTGG	1 cut(s)	812
XmiI	GTMKAC	4 cut(s)	278, 615, 1020, 1049
XmnI	GAANNNNTTC	2 cut(s)	692, 1533
XspI	CTAG	1 cut(s)	657
ZraI	GACGTC	1 cut(s)	94
Zsp2I	ATGCAT	1 cut(s)	342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.