pycom09g17790

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002090
Physical Location & Seq
Reverse (-)
18278163 .. 18281588
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGCGCTTGGCGGATTATTTCGGTCGAGCATTTGCGGCGGTCAGTCCGGCTCAATTTCCGTGGTTGAAGACGTTCAAGGAATCCTCTGTGGCAAAGCTGGTTGATATCCCCCTTTCGCATGTATCTGATGATGTTTACAAGATATCAGTTGAATGGATCGGCCAGCGCTCTACTGAAGCGCTTGGATCGTTTGTGCTATGGTCGTTGGACAGCATTCTTGCTGACCTTGCAACTCATCAAGGAGTAGCCAAGGGATCCAAAAAGGCGGTTCAGCAAGCACCTTCAAAGTCTCAGGTTGCCATATTTGTGGTTTTAGCAATGGTATTGCGACGAAGACCTGAAGTACTGATTAGTTTAGTTCCTGTGATGAGGGGAAGTCCGAAATATGAAGGCCAAGATAAGCTTCCAATCACAGTTTGGTTGGTTGCACAGGCTTCTCAAGGAGATCTGGTTGTTGGGTTGTACTCATGGGTACAATTTCTCTTGCCTATATTGAGTAGCAAGTCAAGTAGTAATCCACTGTGTAGAGACTTAATTTTGCAGTCTGTGGAAAGAATTTTGTCTTCTCCGAAAGCCCGTCCTATTCTATTAAATGGTGCTGTCAGAAAGGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTCTCTTTTCCGACACCTTCTGCCCGAGTTAAGGCAACTGAGAGGTTTGAAGCTGTTTATCCAACTTTGAAAGAGATCGCCCTTGCTGGTTCCCCCAGAAGCAAGACAATGAAGCAAGTCACACAGCAGATATTAAAGAATTCAGTTAAAGCTGTTAATGAGGGCATACCTGATCTGTCTAAGGAAGCAAGCGGCTTATTTATTTGGTGTTTGACGCAAAACCCTGAATGTTACAGGCAATGGGACATACTTTATCTGGACAATCTCGATGCAAGTGTTGTTCTCCTGAAAAAGCTATCTGACGAGTGGAAGGAGCAATCTGTTAAACATGCTTCTCTTGACCCCTTGAGGGAAACTTTAAAGAGTTTCAGGGAGAAGAATGATAAAGCATTGGCAGCGGGGGGAGCTGATGCTGCCCGCCATTCACTGTTGAAGGATGCAGACAAATACTGCAAACAGATTTTGGGACGATTGTCGCAAGGCCATGTATGCATGAAGAGCATGGTACTTGTTTCTGTTGCCCTCGCTGTGGGTGCTGCGGTCATGTCCCAAAACATACAACCTGGGGACCTTAAGAAATTTGTAGCTATGTTTAACTTACCCCCAACTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

423

Amino Acids

46.43

Weight (kDa)

9.51

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 139 - 397 3.1e-19 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 11, 35, 38, 266, 846, 1052, 1072, 1193
AclWI GGATC 4 cut(s) 164, 193, 249, 262
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 3 cut(s) 555, 793, 1232
AcuI CTGAAG 2 cut(s) 195, 360
AdeI CACNNNGTG 1 cut(s) 622
AfaI GTAC 4 cut(s) 345, 464, 474, 1161
AfeI AGCGCT 2 cut(s) 167, 180
AfiI CCNNNNNNNGG 3 cut(s) 685, 1003, 1183
AflII CTTAAG 1 cut(s) 1226
AgsI TTSAA 7 cut(s) 67, 76, 152, 285, 704, 724, 1087
AjnI CCWGG 1 cut(s) 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 7 cut(s) 97, 403, 707, 806, 949, 1061, 1241
AluI AGCT 7 cut(s) 97, 403, 707, 806, 949, 1061, 1241
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 3 cut(s) 294, 522, 661
AlwI GGATC 4 cut(s) 164, 193, 249, 262
Ama87I CYCGRG 1 cut(s) 678
Aor51HI AGCGCT 2 cut(s) 167, 180
AoxI GGCC 3 cut(s) 160, 391, 1135
ApeKI GCWGC 3 cut(s) 1049, 1067, 1190
ApoI RAATTY 3 cut(s) 555, 793, 1232
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 3 cut(s) 6, 168, 181
AspS9I GGNCC 1 cut(s) 1222
AvaI CYCGRG 1 cut(s) 678
AvaII GGWCC 1 cut(s) 1222
BamHI GGATCC 1 cut(s) 254
BbsI GAAGAC 3 cut(s) 74, 340, 555
Bbv12I GWGCWC 1 cut(s) 618
BbvI GCAGC 3 cut(s) 1054, 1061, 1177
BccI CCATC 1 cut(s) 637
BciT130I CCWGG 1 cut(s) 1218
BcoDI GTCTC 3 cut(s) 294, 522, 661
BfoI RGCGCY 2 cut(s) 169, 182
BfrI CTTAAG 1 cut(s) 1226
BglII AGATCT 1 cut(s) 445
BisI GCNGC 5 cut(s) 36, 847, 1050, 1068, 1191
BlsI GCNGC 5 cut(s) 37, 848, 1051, 1069, 1192
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 1 cut(s) 1218
Bme18I GGWCC 1 cut(s) 1222
BmeT110I CYCGRG 1 cut(s) 678
BmgT120I GGNCC 1 cut(s) 1222
BmiI GGNNCC 3 cut(s) 256, 745, 1223
BmrFI CCNGG 1 cut(s) 1218
BmsI GCATC 3 cut(s) 913, 1054, 1081
BoxI GACNNNNGTC 1 cut(s) 1126
BpiI GAAGAC 3 cut(s) 74, 340, 555
BpuEI CTTGAG 2 cut(s) 423, 1021
BsaJI CCNNGG 3 cut(s) 59, 249, 1217
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 1003, 1183
Bse3DI GCAATG 2 cut(s) 324, 899
BseBI CCWGG 1 cut(s) 1218
BseDI CCNNGG 3 cut(s) 59, 249, 1217
BseGI GGATG 1 cut(s) 1096
BseLI CCNNNNNNNGG 3 cut(s) 685, 1003, 1183
BseMI GCAATG 2 cut(s) 324, 899
BseMII CTCAG 2 cut(s) 305, 684
BseXI GCAGC 3 cut(s) 1054, 1061, 1177
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 162, 393, 1137
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 678
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 4 cut(s) 911, 1134, 1186, 1235
BslI CCNNNNNNNGG 3 cut(s) 685, 1003, 1183
BsmAI GTCTC 3 cut(s) 294, 522, 661
BsmFI GGGAC 4 cut(s) 911, 1134, 1186, 1235
BsmI GAATGC 1 cut(s) 213
BsnI GGCC 3 cut(s) 162, 393, 1137
BsoBI CYCGRG 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 618
Bsp143I GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
BspACI CCGC 8 cut(s) 11, 35, 38, 266, 846, 1052, 1072, 1193
BspANI GGCC 3 cut(s) 162, 393, 1137
BspCNI CTCAG 2 cut(s) 304, 685
BspLI GGNNCC 3 cut(s) 256, 745, 1223
BspPI GGATC 4 cut(s) 164, 193, 249, 262
BspQI GCTCTTC 1 cut(s) 1145
BspTI CTTAAG 1 cut(s) 1226
BsrDI GCAATG 2 cut(s) 324, 899
BssECI CCNNGG 3 cut(s) 59, 249, 1217
BssMI GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 1218
Bst4CI ACNGT 3 cut(s) 415, 522, 1083
Bst6I CTCTTC 1 cut(s) 1145
BstAFI CTTAAG 1 cut(s) 1226
BstAPI GCANNNNNTGC 1 cut(s) 622
BstC8I GCNNGC 4 cut(s) 164, 276, 844, 1072
BstDEI CTNAG 3 cut(s) 291, 693, 834
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 1 cut(s) 1096
BstH2I RGCGCY 2 cut(s) 169, 182
BstHHI GCGC 3 cut(s) 6, 168, 181
BstKTI GATC 7 cut(s) 159, 188, 257, 448, 643, 732, 829
BstMAI GTCTC 3 cut(s) 294, 522, 661
BstMBI GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 9 cut(s) 35, 227, 622, 1049, 1058, 1067, 1143, 1152, 1187
BstNI CCWGG 1 cut(s) 1218
BstNSI RCATGY 2 cut(s) 122, 986
BstPAI GACNNNNGTC 1 cut(s) 1126
BstSCI CCNGG 1 cut(s) 1216
BstV1I GCAGC 3 cut(s) 1054, 1061, 1177
BstV2I GAAGAC 3 cut(s) 74, 340, 555
BstX2I RGATCY 2 cut(s) 254, 445
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 2 cut(s) 254, 445
BsuRI GGCC 3 cut(s) 162, 393, 1137
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 518, 1079
Cac8I GCNNGC 4 cut(s) 164, 276, 844, 1072
CfoI GCGC 3 cut(s) 6, 168, 181
Cfr13I GGNCC 1 cut(s) 1222
CseI GACGC 1 cut(s) 877
Csp6I GTAC 4 cut(s) 344, 463, 473, 1160
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 7 cut(s) 119, 468, 983, 1139, 1147, 1156, 1198
CviQI GTAC 4 cut(s) 344, 463, 473, 1160
DdeI CTNAG 3 cut(s) 291, 693, 834
DpnI GATC 7 cut(s) 158, 187, 256, 447, 642, 731, 828
DpnII GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
DraI TTTAAA 1 cut(s) 1014
DraIII CACNNNGTG 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 1145
EarI CTCTTC 1 cut(s) 1145
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 249
Eco32I GATATC 2 cut(s) 106, 144
Eco47I GGWCC 1 cut(s) 1222
Eco47III AGCGCT 2 cut(s) 167, 180
Eco57I CTGAAG 2 cut(s) 195, 360
Eco88I CYCGRG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 1222
EcoRI GAATTC 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 1216
EcoRV GATATC 2 cut(s) 106, 144
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 1148
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 7 cut(s) 122, 471, 986, 1142, 1150, 1159, 1201
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 4 cut(s) 911, 1134, 1186, 1235
FatI CATG 7 cut(s) 118, 467, 982, 1138, 1146, 1155, 1197
FauI CCCGC 2 cut(s) 1045, 1079
Fnu4HI GCNGC 5 cut(s) 36, 847, 1050, 1068, 1191
FokI GGATG 1 cut(s) 1103
Fsp4HI GCNGC 5 cut(s) 36, 847, 1050, 1068, 1191
GlaI GCGC 3 cut(s) 5, 167, 180
GluI GCNGC 5 cut(s) 36, 847, 1050, 1068, 1191
HaeII RGCGCY 2 cut(s) 169, 182
HaeIII GGCC 3 cut(s) 162, 393, 1137
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 877
HhaI GCGC 3 cut(s) 6, 168, 181
Hin1II CATG 7 cut(s) 122, 471, 986, 1142, 1150, 1159, 1201
Hin6I GCGC 3 cut(s) 4, 166, 179
HinP1I GCGC 3 cut(s) 4, 166, 179
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 2 cut(s) 80, 654
HpaII CCGG 1 cut(s) 47
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 7 cut(s) 127, 381, 570, 605, 666, 955, 1268
Hpy188III TCNNGA 5 cut(s) 646, 911, 920, 940, 992
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 5 cut(s) 291, 383, 681, 958, 1081
HpyCH4III ACNGT 3 cut(s) 415, 522, 1083
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 7 cut(s) 230, 428, 541, 926, 1094, 1107, 1146
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 227, 622, 1049, 1058, 1067, 1143, 1152, 1187
HpyF3I CTNAG 3 cut(s) 291, 693, 834
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 7 cut(s) 122, 471, 986, 1142, 1150, 1159, 1201
HspAI GCGC 3 cut(s) 4, 166, 179
Kzo9I GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
LguI GCTCTTC 1 cut(s) 1145
LmnI GCTCC 3 cut(s) 613, 967, 1058
Lsp1109I GCAGC 3 cut(s) 1054, 1061, 1177
LweI GCATC 3 cut(s) 913, 1054, 1081
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 2 cut(s) 772, 884
MalI GATC 7 cut(s) 158, 187, 256, 447, 642, 731, 828
MboI GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
MboII GAAGA 5 cut(s) 79, 345, 555, 1042, 1162
MflI RGATCY 2 cut(s) 254, 445
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 6 cut(s) 53, 476, 534, 555, 793, 1232
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 3 cut(s) 186, 689, 740
MnlI CCTC 6 cut(s) 94, 363, 690, 808, 996, 1187
Mph1103I ATGCAT 1 cut(s) 1148
MroXI GAANNNNTTC 1 cut(s) 71
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 1052
MspCI CTTAAG 1 cut(s) 1226
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 1218
Mva1269I GAATGC 1 cut(s) 213
MvaI CCWGG 1 cut(s) 1218
MwoI GCNNNNNNNGC 9 cut(s) 35, 227, 622, 1049, 1058, 1067, 1143, 1152, 1187
NdeII GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
NlaIII CATG 7 cut(s) 122, 471, 986, 1142, 1150, 1159, 1201
NlaIV GGNNCC 3 cut(s) 256, 745, 1223
NmuCI GTSAC 1 cut(s) 772
NsiI ATGCAT 1 cut(s) 1148
NspI RCATGY 2 cut(s) 122, 986
PciSI GCTCTTC 1 cut(s) 1145
PctI GAATGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 5 cut(s) 37, 848, 1051, 1069, 1192
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 1222
PshAI GACNNNNGTC 1 cut(s) 1126
Psp5II RGGWCCY 1 cut(s) 1222
Psp6I CCWGG 1 cut(s) 1216
PspGI CCWGG 1 cut(s) 1216
PspN4I GGNNCC 3 cut(s) 256, 745, 1223
PspPI GGNCC 1 cut(s) 1222
PspPPI RGGWCCY 1 cut(s) 1222
PsuI RGATCY 2 cut(s) 254, 445
RsaI GTAC 4 cut(s) 345, 464, 474, 1161
RsaNI GTAC 4 cut(s) 344, 463, 473, 1160
RseI CAYNNNNRTG 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 1145
SatI GCNGC 5 cut(s) 36, 847, 1050, 1068, 1191
Sau3AI GATC 7 cut(s) 156, 185, 254, 445, 640, 729, 826
Sau96I GGNCC 1 cut(s) 1222
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 1 cut(s) 663
ScrFI CCNGG 1 cut(s) 1218
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 3 cut(s) 913, 1054, 1081
SinI GGWCC 1 cut(s) 1222
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 3 cut(s) 438, 1000, 1226
SmoI CTYRAG 3 cut(s) 438, 1000, 1226
Sse9I AATT 6 cut(s) 53, 476, 534, 555, 793, 1232
SsiI CCGC 8 cut(s) 11, 35, 38, 266, 846, 1052, 1072, 1193
StyD4I CCNGG 1 cut(s) 1216
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 3 cut(s) 415, 522, 1083
TaiI ACGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 25, 921
TaqII GACCGA 1 cut(s) 11
TasI AATT 6 cut(s) 53, 476, 534, 555, 793, 1232
TatI WGTACW 2 cut(s) 343, 462
TauI GCSGC 2 cut(s) 38, 849
TfiI GAWTC 1 cut(s) 80
TscAI CASTG 2 cut(s) 525, 1086
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 3 cut(s) 1049, 1067, 1190
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 3 cut(s) 402, 779, 1163
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 2 cut(s) 525, 1086
Vha464I CTTAAG 1 cut(s) 1226
VpaK11BI GGWCC 1 cut(s) 1222
XapI RAATTY 3 cut(s) 555, 793, 1232
XceI RCATGY 2 cut(s) 122, 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.