pycom10g10760

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003977
Physical Location & Seq
Forward (+)
14221277 .. 14222427
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGTCTAAGGAAGTAATCGAGGAGGGACAGGCCCGCCACCATGGGAAGGACTACGTTGACCCACCGCCAGCTCCTCTCTTTGACACGGACGAGCTCAAGCGCTGGTCCTTCTATAGGGCCCTCATCGCCGAGTTCATCGCCACTCTCCTCTTCCTCTACGTCACCGTAGCCACCGTCATCGGCCACAAGTCCTCACCCGGCGACTGCGGCGGCGTTGGCCTTCTGGGCATCGCCTGGGCCTTCGGCGGCATGATTTTCGTCCTCGTCTACTGTACCGCCGGAATCTCTGGTGGCCACATTAACCCAGCCGTTACGTTTGGCCTTTTCTTGGCCCGCAAGGTCTCGCTGATCAGAGCCGTGGCTTACATCGTTGCCCAGTGCTTGGGAGCCATCGCAGGCGTTGGGCTGGTGAAGGCCTTCCAGAAGCACAACTACAACTCCCTCGGCGGCGGCGCCAACTCCGTGTCTCCTGGCTACAGCAAGGGCACTGCTCTGGGTGCTGAGATCATCGGCACCTTCGTCCTCGTTTACACCGTCTTCTCAGCCACTGACCCCAAAAGGAGCGCACGTGACTCCCACGTGCCTGTTTTGGCTCCACTGCCCATTGGGTTTGCTGTGTTTGTTGTGCACCTGGCAACAATCCCCATCACCGGAACTGGAATCAACCCAGCAAGGAGCTTCGGCGCTGCTGTGATATACAACAATGAGAAAGTCTGGGATGACCACTGGATCTTCTGGGTCGGACCATTTGTGGGAGCACTGGGAGCGGCGGCGTACCACCAGTACATCCTGAGGGCGGCGGCGATCAAGGCGTTGGGATCATTCCGCAGCAACCCCACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

282

Amino Acids

29.81

Weight (kDa)

9.08

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 259 2e-83 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 452, 512
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 767
AccI GTMKAC 1 cut(s) 267
AclWI GGATC 2 cut(s) 737, 826
AcoI YGGCCR 2 cut(s) 181, 292
AcvI CACGTG 2 cut(s) 569, 580
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 274, 776, 785
AfeI AGCGCT 1 cut(s) 101
AfiI CCNNNNNNNGG 7 cut(s) 46, 114, 328, 382, 650, 752, 796
AjnI CCWGG 3 cut(s) 233, 469, 630
AluBI AGCT 3 cut(s) 71, 94, 678
AluI AGCT 3 cut(s) 71, 94, 678
Alw21I GWGCWC 3 cut(s) 96, 630, 760
Alw26I GTCTC 2 cut(s) 346, 471
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 2 cut(s) 737, 826
AlwNI CAGNNNCTG 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 101
AoxI GGCC 9 cut(s) 30, 117, 181, 217, 237, 292, 319, 330, 414
ApaI GGGCCC 1 cut(s) 121
ApaLI GTGCAC 1 cut(s) 626
ApeKI GCWGC 2 cut(s) 686, 828
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 4 cut(s) 102, 455, 566, 686
AspS9I GGNCC 7 cut(s) 31, 105, 117, 118, 237, 331, 743
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 4 cut(s) 154, 186, 421, 640
AvaII GGWCC 2 cut(s) 105, 743
AxyI CCTNAGG 1 cut(s) 791
BaeGI GKGCMC 3 cut(s) 121, 488, 630
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 2 cut(s) 452, 512
BanII GRGCYC 2 cut(s) 96, 121
BarI GAAGNNNNNNTAC 2 cut(s) 416, 448
BbrPI CACGTG 2 cut(s) 569, 580
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 3 cut(s) 96, 630, 760
BbvI GCAGC 2 cut(s) 673, 840
BccI CCATC 2 cut(s) 398, 653
BceAI ACGGC 2 cut(s) 293, 341
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 3 cut(s) 235, 471, 632
BclI TGATCA 1 cut(s) 348
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 346, 471
BfmI CTRYAG 2 cut(s) 112, 475
BfoI RGCGCY 3 cut(s) 103, 456, 687
BglI GCCNNNNNGGC 1 cut(s) 225
Bme1390I CCNGG 4 cut(s) 198, 235, 471, 632
Bme18I GGWCC 2 cut(s) 105, 743
BmgT120I GGNCC 7 cut(s) 31, 105, 117, 118, 237, 331, 743
BmiI GGNNCC 5 cut(s) 119, 388, 454, 514, 594
BmrFI CCNGG 4 cut(s) 198, 235, 471, 632
BmrI ACTGGG 2 cut(s) 370, 770
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 2 cut(s) 370, 770
BpiI GAAGAC 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 198
BsaAI YACGTR 2 cut(s) 569, 580
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 40, 234, 357, 442
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 553, 583
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 114, 328, 382, 650, 752, 796
Bse1I ACTGG 5 cut(s) 376, 661, 731, 765, 781
Bse21I CCTNAGG 1 cut(s) 791
BseBI CCWGG 3 cut(s) 235, 471, 632
BseDI CCNNGG 4 cut(s) 40, 234, 357, 442
BseGI GGATG 2 cut(s) 724, 786
BseLI CCNNNNNNNGG 7 cut(s) 46, 114, 328, 382, 650, 752, 796
BseMII CTCAG 3 cut(s) 492, 555, 782
BseNI ACTGG 5 cut(s) 376, 661, 731, 765, 781
BseRI GAGGAG 3 cut(s) 35, 63, 137
BseSI GKGCMC 3 cut(s) 121, 488, 630
BseXI GCAGC 2 cut(s) 673, 840
BseYI CCCAGC 2 cut(s) 304, 667
BshFI GGCC 9 cut(s) 32, 119, 183, 219, 239, 294, 321, 332, 416
BshNI GGYRCC 2 cut(s) 452, 512
BsiHKAI GWGCWC 3 cut(s) 96, 630, 760
BsiSI CCGG 3 cut(s) 198, 279, 651
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 7 cut(s) 46, 114, 328, 382, 650, 752, 796
BsmAI GTCTC 2 cut(s) 346, 471
BsmFI GGGAC 1 cut(s) 39
BsnI GGCC 9 cut(s) 32, 119, 183, 219, 239, 294, 321, 332, 416
Bso31I GGTCTC 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 5 cut(s) 96, 121, 488, 630, 760
Bsp143I GATC 5 cut(s) 348, 504, 729, 804, 818
Bsp19I CCATGG 1 cut(s) 40
BspANI GGCC 9 cut(s) 32, 119, 183, 219, 239, 294, 321, 332, 416
BspCNI CTCAG 3 cut(s) 493, 554, 783
BspLI GGNNCC 5 cut(s) 119, 388, 454, 514, 594
BspPI GGATC 2 cut(s) 737, 826
BspT107I GGYRCC 2 cut(s) 452, 512
BspTNI GGTCTC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 767
BsrI ACTGG 5 cut(s) 376, 661, 731, 765, 781
BssECI CCNNGG 4 cut(s) 40, 234, 357, 442
BssMI GATC 5 cut(s) 348, 504, 729, 804, 818
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 235, 471, 632
Bst4CI ACNGT 4 cut(s) 166, 175, 272, 535
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 453
BstBAI YACGTR 2 cut(s) 569, 580
BstC8I GCNNGC 4 cut(s) 34, 69, 334, 397
BstDEI CTNAG 4 cut(s) 6, 501, 541, 791
BstDSI CCRYGG 2 cut(s) 40, 357
BstENI CCTNNNNNAGG 1 cut(s) 112
BstF5I GGATG 2 cut(s) 724, 786
BstH2I RGCGCY 3 cut(s) 103, 456, 687
BstHHI GCGC 4 cut(s) 102, 455, 566, 686
BstKTI GATC 5 cut(s) 351, 507, 732, 807, 821
BstMAI GTCTC 2 cut(s) 346, 471
BstMBI GATC 5 cut(s) 348, 504, 729, 804, 818
BstMWI GCNNNNNNNGC 7 cut(s) 125, 207, 216, 225, 497, 764, 809
BstNI CCWGG 3 cut(s) 235, 471, 632
BstSCI CCNGG 4 cut(s) 196, 233, 469, 630
BstSFI CTRYAG 2 cut(s) 112, 475
BstSLI GKGCMC 3 cut(s) 121, 488, 630
BstV1I GCAGC 2 cut(s) 673, 840
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 729
BstYI RGATCY 1 cut(s) 729
Bsu36I CCTNAGG 1 cut(s) 791
BsuRI GGCC 9 cut(s) 32, 119, 183, 219, 239, 294, 321, 332, 416
BtgI CCRYGG 2 cut(s) 40, 357
BtgZI GCGATG 4 cut(s) 109, 121, 214, 376
BtsCI GGATG 2 cut(s) 724, 786
BtsI GCAGTG 2 cut(s) 486, 596
BtsIMutI CAGTG 6 cut(s) 383, 486, 546, 596, 724, 758
Cac8I GCNNGC 4 cut(s) 34, 69, 334, 397
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 4 cut(s) 102, 455, 566, 686
Cfr13I GGNCC 7 cut(s) 31, 105, 117, 118, 237, 331, 743
Csp6I GTAC 3 cut(s) 273, 775, 784
CviAII CATG 2 cut(s) 41, 250
CviQI GTAC 3 cut(s) 273, 775, 784
DdeI CTNAG 4 cut(s) 6, 501, 541, 791
DinI GGCGCC 1 cut(s) 454
DpnI GATC 5 cut(s) 350, 506, 731, 806, 820
DpnII GATC 5 cut(s) 348, 504, 729, 804, 818
EaeI YGGCCR 2 cut(s) 181, 292
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 40
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 2 cut(s) 96, 121
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 105, 743
Eco47III AGCGCT 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 94
Eco72I CACGTG 2 cut(s) 569, 580
Eco81I CCTNAGG 1 cut(s) 791
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 112
EcoO109I RGGNCCY 2 cut(s) 117, 118
EcoRII CCWGG 3 cut(s) 233, 469, 630
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 2 cut(s) 96, 121
EgeI GGCGCC 1 cut(s) 454
EheI GGCGCC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 44, 253
FaiI YATR 4 cut(s) 42, 114, 251, 697
FaqI GGGAC 1 cut(s) 39
FatI CATG 2 cut(s) 40, 249
FauI CCCGC 2 cut(s) 41, 341
FbaI TGATCA 1 cut(s) 348
FblI GTMKAC 1 cut(s) 267
FokI GGATG 2 cut(s) 731, 773
FriOI GRGCYC 2 cut(s) 96, 121
GlaI GCGC 4 cut(s) 101, 454, 565, 685
GsaI CCCAGC 2 cut(s) 308, 671
HaeII RGCGCY 3 cut(s) 103, 456, 687
HaeIII GGCC 9 cut(s) 32, 119, 183, 219, 239, 294, 321, 332, 416
HapII CCGG 3 cut(s) 198, 279, 651
HhaI GCGC 4 cut(s) 102, 455, 566, 686
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 2 cut(s) 44, 253
Hin6I GCGC 4 cut(s) 100, 453, 564, 684
HinP1I GCGC 4 cut(s) 100, 453, 564, 684
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 3 cut(s) 282, 572, 660
HpaII CCGG 3 cut(s) 198, 279, 651
HphI GGTGA 4 cut(s) 154, 186, 421, 640
Hpy166II GTNNAC 4 cut(s) 58, 268, 529, 628
Hpy188I TCNGA 2 cut(s) 353, 743
Hpy188III TCNNGA 2 cut(s) 421, 790
Hpy8I GTNNAC 4 cut(s) 58, 268, 529, 628
HpyAV CCTTC 7 cut(s) 40, 118, 230, 250, 406, 427, 526
HpyCH4III ACNGT 4 cut(s) 166, 175, 272, 535
HpyCH4IV ACGT 5 cut(s) 54, 159, 314, 568, 579
HpyCH4V TGCA 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 207, 216, 225, 497, 764, 809
HpyF3I CTNAG 4 cut(s) 6, 501, 541, 791
HpySE526I ACGT 5 cut(s) 54, 159, 314, 568, 579
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 2 cut(s) 44, 253
HspAI GCGC 4 cut(s) 100, 453, 564, 684
KasI GGCGCC 1 cut(s) 452
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 5 cut(s) 348, 504, 729, 804, 818
LmnI GCTCC 7 cut(s) 76, 386, 561, 598, 675, 755, 764
Lsp1109I GCAGC 2 cut(s) 673, 840
LweI GCATC 1 cut(s) 237
MaeII ACGT 5 cut(s) 54, 159, 314, 568, 579
MaeIII GTNAC 3 cut(s) 160, 310, 569
MalI GATC 5 cut(s) 350, 506, 731, 806, 820
MbiI CCGCTC 1 cut(s) 767
MboI GATC 5 cut(s) 348, 504, 729, 804, 818
MboII GAAGA 3 cut(s) 142, 529, 724
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 5 cut(s) 96, 121, 488, 630, 760
MlsI TGGCCA 1 cut(s) 294
MluNI TGGCCA 1 cut(s) 294
Mly113I GGCGCC 1 cut(s) 453
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 721
Mox20I TGGCCA 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 1 cut(s) 294
MseI TTAA 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 3 cut(s) 198, 279, 651
MspR9I CCNGG 4 cut(s) 198, 235, 471, 632
MvaI CCWGG 3 cut(s) 235, 471, 632
MwoI GCNNNNNNNGC 7 cut(s) 125, 207, 216, 225, 497, 764, 809
NarI GGCGCC 1 cut(s) 453
NciI CCSGG 1 cut(s) 198
NcoI CCATGG 1 cut(s) 40
NdeII GATC 5 cut(s) 348, 504, 729, 804, 818
NlaIII CATG 2 cut(s) 44, 253
NlaIV GGNNCC 5 cut(s) 119, 388, 454, 514, 594
NmeAIII GCCGAG 2 cut(s) 154, 423
NmuCI GTSAC 2 cut(s) 160, 569
PceI AGGCCT 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 516, 531
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 282, 660
PflMI CCANNNNNTGG 1 cut(s) 382
PleI GAGTC 1 cut(s) 566
PluTI GGCGCC 1 cut(s) 456
PmaCI CACGTG 2 cut(s) 569, 580
PmlI CACGTG 2 cut(s) 569, 580
PpsI GAGTC 1 cut(s) 566
Ppu21I YACGTR 2 cut(s) 569, 580
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 3 cut(s) 233, 469, 630
PspCI CACGTG 2 cut(s) 569, 580
PspFI CCCAGC 2 cut(s) 304, 667
PspGI CCWGG 3 cut(s) 233, 469, 630
PspN4I GGNNCC 5 cut(s) 119, 388, 454, 514, 594
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 7 cut(s) 31, 105, 117, 118, 237, 331, 743
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 729
RsaI GTAC 3 cut(s) 274, 776, 785
RsaNI GTAC 3 cut(s) 273, 775, 784
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 300
Sau3AI GATC 5 cut(s) 348, 504, 729, 804, 818
Sau96I GGNCC 7 cut(s) 31, 105, 117, 118, 237, 331, 743
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 4 cut(s) 198, 235, 471, 632
SduI GDGCHC 5 cut(s) 96, 121, 488, 630, 760
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 2 cut(s) 112, 475
SfoI GGCGCC 1 cut(s) 454
SinI GGWCC 2 cut(s) 105, 743
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 416
SspDI GGCGCC 1 cut(s) 452
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 4 cut(s) 196, 233, 469, 630
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 4 cut(s) 166, 175, 272, 535
TaiI ACGT 5 cut(s) 57, 162, 317, 571, 582
TaqI TCGA 1 cut(s) 18
TatI WGTACW 1 cut(s) 783
TauI GCSGC 9 cut(s) 210, 213, 249, 450, 453, 770, 773, 800, 803
TfiI GAWTC 2 cut(s) 282, 660
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 6 cut(s) 383, 493, 553, 603, 731, 765
TseFI GTSAC 2 cut(s) 160, 569
TseI GCWGC 2 cut(s) 686, 828
Tsp45I GTSAC 2 cut(s) 160, 569
TspDTI ATGAA 1 cut(s) 124
TspGWI ACGGA 2 cut(s) 101, 451
TspRI CASTG 6 cut(s) 383, 493, 553, 603, 731, 765
Van91I CCANNNNNTGG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 105, 743
XagI CCTNNNNNAGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.