pycom10g10880

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002066
Physical Location & Seq
Forward (+)
14385437 .. 14388653
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGAATCTGGCCCCTATGGTACTTTTGTTGGTGTTTATGACGGCCATGGGGGGCCGGAGACGTCACGCTATATCAATCGATCACCTCTTTCAACATCTCAAGAGATTTAGTTCGGAGCAGCAGTCAATGTCTGTGGATGTAATACGAAAAGCATTTCAAGCAACAGAAGAGGGCTTTCTTAACGTAGTTACTAGGCAGTGGCCTATGAAACCACAGATTGCAGCGGTAGGATCGTGCTGCCTTGTCGGTGTTATTTGTAATGAAACTCTTTATATCGCCAACTGTGGTGATTCCCGTGCTGTTTTGGGGAGAATAGTGAAGGCAACAGGGGAGGTGCTCTCTATTCAGTTGTCACAAGAGCACAATGCCTGTATAGAATCTGTGAGACAGGAGCTGCAGTCTTTGCACCCTGATGATCCACATATCGTGGTTTTGAAGCATAATGTATGGCGTGTGAAAGGCCTAATACAGATATCTAGATCTATTGGTGATGTGTATTTAAAAAAGGCTGAATTTAACAGGGAGCCTTTGTATTCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATTTTGAGCGCTGATCCAGCAATTTCAGTGATCCAATTGCAGCCACACGATCAATTTATTATATTTGCATCTGATGGGCTATGGGAACACCTCACCAATCAGGAAGCAGTGGACATAGTTCAAAAACATCCACGAAGTGGAAGTGCAAAAAGGCTGGTGAAAACCGCCCTGCAAGAAGCAGCAAAAAAGAGAGAAATGAGGTATTCGGACTTGAAGAAGATTGACCGTGGGGTTCGCCGCCATTTCCACGATGACATAACCGTGATTGTCGTGTTTCTCGACTCAAACCTTGTGAGCAAGGCTAGTTCTGTCAAGGGCCCGAATCTATCGGTGAGAGGAGGTGGAATCAACCTGCCTCCTAACACCCTGGCTCCATACGCCACGACAACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

326

Amino Acids

36.34

Weight (kDa)

9.53

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 15 - 252 4e-35 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 930
AccB7I CCANNNNNTGG 1 cut(s) 707
AciI CCGC 3 cut(s) 224, 735, 808
AclWI GGATC 4 cut(s) 238, 410, 578, 595
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 61
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 1 cut(s) 21
AfeI AGCGCT 1 cut(s) 580
AfiI CCNNNNNNNGG 1 cut(s) 707
AgsI TTSAA 5 cut(s) 92, 158, 436, 692, 784
AjnI CCWGG 1 cut(s) 936
AluBI AGCT 2 cut(s) 394, 965
AluI AGCT 2 cut(s) 394, 965
Alw21I GWGCWC 2 cut(s) 339, 363
Alw26I GTCTC 2 cut(s) 52, 379
AlwI GGATC 4 cut(s) 238, 410, 578, 595
AlwNI CAGNNNCTG 1 cut(s) 394
Aor51HI AGCGCT 1 cut(s) 580
AoxI GGCC 6 cut(s) 9, 42, 52, 200, 460, 886
ApaI GGGCCC 1 cut(s) 890
ApeKI GCWGC 6 cut(s) 118, 221, 237, 394, 610, 749
ApoI RAATTY 1 cut(s) 512
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 5 cut(s) 10, 52, 553, 886, 887
AsuHPI GGTGA 6 cut(s) 74, 299, 500, 655, 739, 913
AvaII GGWCC 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 890
BaeI ACNNNNGTAYC 2 cut(s) 11, 44
BanII GRGCYC 1 cut(s) 890
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 2 cut(s) 339, 363
BbvI GCAGC 6 cut(s) 130, 224, 233, 381, 622, 761
BccI CCATC 1 cut(s) 638
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 938
BcoDI GTCTC 2 cut(s) 52, 379
BfaI CTAG 3 cut(s) 192, 477, 873
BfmI CTRYAG 1 cut(s) 395
BfoI RGCGCY 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 930
BglII AGATCT 1 cut(s) 479
BisI GCNGC 7 cut(s) 119, 222, 238, 395, 611, 750, 808
BlsI GCNGC 7 cut(s) 120, 223, 239, 396, 612, 751, 809
Bme1390I CCNGG 1 cut(s) 938
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 5 cut(s) 10, 52, 553, 886, 887
BmiI GGNNCC 7 cut(s) 12, 53, 525, 554, 555, 888, 942
BmrFI CCNGG 1 cut(s) 938
BmsI GCATC 1 cut(s) 647
BpiI GAAGAC 1 cut(s) 539
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpuEI CTTGAG 1 cut(s) 83
Bsa29I ATCGAT 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 45, 796, 936
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 383, 413, 515, 545, 925, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 707
BseBI CCWGG 1 cut(s) 938
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 45, 796, 936
BseGI GGATG 2 cut(s) 142, 697
BseLI CCNNNNNNNGG 1 cut(s) 707
BseRI GAGGAG 1 cut(s) 921
BseSI GKGCMC 1 cut(s) 890
BseXI GCAGC 6 cut(s) 130, 224, 233, 381, 622, 761
BshFI GGCC 6 cut(s) 11, 44, 54, 202, 462, 888
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 339, 363
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 2 cut(s) 52, 379
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 6 cut(s) 11, 44, 54, 202, 462, 888
Bsp120I GGGCCC 1 cut(s) 886
Bsp1286I GDGCHC 3 cut(s) 339, 363, 890
Bsp143I GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 3 cut(s) 224, 735, 808
BspANI GGCC 6 cut(s) 11, 44, 54, 202, 462, 888
BspDI ATCGAT 1 cut(s) 78
BspLI GGNNCC 7 cut(s) 12, 53, 525, 554, 555, 888, 942
BspMAI CTGCAG 1 cut(s) 399
BspMI ACCTGC 1 cut(s) 930
BspPI GGATC 4 cut(s) 238, 410, 578, 595
BssECI CCNNGG 3 cut(s) 45, 796, 936
BssMI GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
BssNI GRCGYC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 938
Bst4CI ACNGT 3 cut(s) 284, 797, 832
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 61
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 967
BstDSI CCRYGG 2 cut(s) 45, 796
BstF5I GGATG 2 cut(s) 142, 697
BstH2I RGCGCY 1 cut(s) 582
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 7 cut(s) 82, 233, 418, 482, 586, 603, 622
BstMAI GTCTC 2 cut(s) 52, 379
BstMBI GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
BstMWI GCNNNNNNNGC 4 cut(s) 158, 403, 587, 947
BstNI CCWGG 1 cut(s) 938
BstSCI CCNGG 1 cut(s) 936
BstSFI CTRYAG 1 cut(s) 395
BstSLI GKGCMC 1 cut(s) 890
BstV1I GCAGC 6 cut(s) 130, 224, 233, 381, 622, 761
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 6 cut(s) 11, 44, 54, 202, 462, 888
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 2 cut(s) 45, 796
BtsCI GGATG 2 cut(s) 142, 697
BtsI GCAGTG 2 cut(s) 203, 684
BtsIMutI CAGTG 3 cut(s) 203, 603, 684
BveI ACCTGC 1 cut(s) 930
Cac8I GCNNGC 1 cut(s) 967
CaiI CAGNNNCTG 1 cut(s) 394
CfoI GCGC 1 cut(s) 581
Cfr13I GGNCC 5 cut(s) 10, 52, 553, 886, 887
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 1 cut(s) 20
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 1 cut(s) 46
CviQI GTAC 1 cut(s) 20
DpnI GATC 7 cut(s) 81, 232, 417, 481, 585, 602, 621
DpnII GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
DraI TTTAAA 1 cut(s) 501
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 462
Eco24I GRGCYC 1 cut(s) 890
Eco32I GATATC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 553
Eco47III AGCGCT 1 cut(s) 580
EcoO109I RGGNCCY 2 cut(s) 553, 886
EcoRII CCWGG 1 cut(s) 936
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 890
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 1 cut(s) 49
FaqI GGGAC 1 cut(s) 566
FatI CATG 1 cut(s) 45
Fnu4HI GCNGC 7 cut(s) 119, 222, 238, 395, 611, 750, 808
FokI GGATG 2 cut(s) 149, 684
FriOI GRGCYC 1 cut(s) 890
Fsp4HI GCNGC 7 cut(s) 119, 222, 238, 395, 611, 750, 808
FspBI CTAG 3 cut(s) 192, 477, 873
GlaI GCGC 1 cut(s) 580
GluI GCNGC 7 cut(s) 119, 222, 238, 395, 611, 750, 808
HaeII RGCGCY 1 cut(s) 582
HaeIII GGCC 6 cut(s) 11, 44, 54, 202, 462, 888
HapII CCGG 1 cut(s) 55
HhaI GCGC 1 cut(s) 581
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 1 cut(s) 49
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HinfI GANTC 6 cut(s) 4, 290, 377, 851, 892, 915
HpaII CCGG 1 cut(s) 55
HphI GGTGA 6 cut(s) 74, 299, 500, 655, 739, 913
Hpy166II GTNNAC 1 cut(s) 682
Hpy188I TCNGA 3 cut(s) 115, 643, 778
Hpy188III TCNNGA 5 cut(s) 100, 477, 551, 671, 848
Hpy8I GTNNAC 1 cut(s) 682
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 3 cut(s) 284, 797, 832
HpyCH4IV ACGT 2 cut(s) 61, 183
HpyCH4V TGCA 7 cut(s) 221, 397, 406, 610, 638, 716, 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 403, 587, 947
HpySE526I ACGT 2 cut(s) 61, 183
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 1 cut(s) 49
HspAI GCGC 1 cut(s) 579
KflI GGGWCCC 1 cut(s) 553
Kzo9I GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
LmnI GCTCC 4 cut(s) 115, 391, 523, 946
Lsp1109I GCAGC 6 cut(s) 130, 224, 233, 381, 622, 761
LweI GCATC 1 cut(s) 647
MaeI CTAG 3 cut(s) 192, 477, 873
MaeII ACGT 2 cut(s) 61, 183
MaeIII GTNAC 3 cut(s) 62, 187, 351
MalI GATC 7 cut(s) 81, 232, 417, 481, 585, 602, 621
MboI GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
MboII GAAGA 4 cut(s) 179, 539, 796, 799
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 3 cut(s) 339, 363, 890
MluCI AATT 5 cut(s) 512, 570, 591, 605, 623
MlyI GAGTC 1 cut(s) 845
MnlI CCTC 8 cut(s) 95, 163, 325, 671, 762, 899, 902, 936
MseI TTAA 3 cut(s) 180, 500, 516
MslI CAYNNNNRTG 2 cut(s) 411, 830
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 938
MunI CAATTG 1 cut(s) 605
MvaI CCWGG 1 cut(s) 938
MwoI GCNNNNNNNGC 4 cut(s) 158, 403, 587, 947
NcoI CCATGG 1 cut(s) 45
NdeII GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 7 cut(s) 12, 53, 525, 554, 555, 888, 942
NmuCI GTSAC 2 cut(s) 62, 351
PceI AGGCCT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 846
PfeI GAWTC 5 cut(s) 4, 290, 377, 892, 915
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 7 cut(s) 120, 223, 239, 396, 612, 751, 809
PleI GAGTC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 845
PpuMI RGGWCCY 1 cut(s) 553
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 936
PspGI CCWGG 1 cut(s) 936
PspN4I GGNNCC 7 cut(s) 12, 53, 525, 554, 555, 888, 942
PspOMI GGGCCC 1 cut(s) 886
PspPI GGNCC 5 cut(s) 10, 52, 553, 886, 887
PspPPI RGGWCCY 1 cut(s) 553
PstI CTGCAG 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 1 cut(s) 479
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 2 cut(s) 411, 830
SaqAI TTAA 3 cut(s) 180, 500, 516
SatI GCNGC 7 cut(s) 119, 222, 238, 395, 611, 750, 808
Sau3AI GATC 7 cut(s) 79, 230, 415, 479, 583, 600, 619
Sau96I GGNCC 5 cut(s) 10, 52, 553, 886, 887
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 1 cut(s) 938
SduI GDGCHC 3 cut(s) 339, 363, 890
SfaNI GCATC 1 cut(s) 647
SfcI CTRYAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 411, 830
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 5 cut(s) 512, 570, 591, 605, 623
SseBI AGGCCT 1 cut(s) 462
SsiI CCGC 3 cut(s) 224, 735, 808
SspMI CTAG 3 cut(s) 192, 477, 873
StuI AGGCCT 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 936
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 3 cut(s) 284, 797, 832
TaiI ACGT 2 cut(s) 64, 186
TaqI TCGA 2 cut(s) 78, 849
TasI AATT 5 cut(s) 512, 570, 591, 605, 623
TauI GCSGC 1 cut(s) 810
TfiI GAWTC 5 cut(s) 4, 290, 377, 892, 915
Tru1I TTAA 3 cut(s) 180, 500, 516
Tru9I TTAA 3 cut(s) 180, 500, 516
TscAI CASTG 3 cut(s) 203, 603, 684
TseFI GTSAC 2 cut(s) 62, 351
TseI GCWGC 6 cut(s) 118, 221, 237, 394, 610, 749
Tsp45I GTSAC 2 cut(s) 62, 351
TspDTI ATGAA 3 cut(s) 17, 221, 276
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 3 cut(s) 203, 603, 684
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 476
XspI CTAG 3 cut(s) 192, 477, 873
ZraI GACGTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.