pycom111g05040

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0003069
Physical Location & Seq
Forward (+)
3206697 .. 3207986
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGGTGTAATTCAATACCATCAACTCCCAGTCTTAAAGATCAAGTGTCACACCAAAGCGGTTTTTTTTTTTCGTGCGAAGTTGGAGGCCAATGAAGTACAGGCTTGTCACCCCTCAATGAGCCTTGTCCGCCAGGGGAACCATTCAAGTTTAGGTCACCGGGTGTATGTGTCTGCAATTGATGTCAACGATGTTCCTCCGAATGAATATCTGCATGGAGTAACTTGTGGACAAAATATTATTTCTCTTCAGGGGCTGAGGTACCAAGAAAGCCGACAAGGAAGATTGCATTGGCTTGAAGCAGTGTCACTTTGCAACGGTGAATCTTCCAAAGAAGGTGAAGATTTGGAGGCACCGGTTGTAGTTGAAACAGATATATATGGGGAAGATTCCATTGAAAGAAGACGTGAGGACTCACCTCATGAGGGCCTTATCAAAGCAGCAGCTGAGGCTCCAGCTGCCATCTCATCATCTCAAACTCACGATATGCAGGACGATGCCACTGGTCGCAAAATGGATACTTTGCAAACCGACTCCCTTCGTTGGTTTGCAGAAATAATTTCTTCATGTGACGGAAGTCTTGACAATGGAAGTGCGGCAGAGGTTAAGCGAACTGCAGGTGAAGAGTCCTTTCCCGATGTGACGGATTACTTTGAGCACATGACATTAAATTTGGTGGAAACCAAGGAAGACCGGCCCTGCTATGTGCCTCCAAATTCAGACAACCTAATAGAAGAAGTGACATTGTCAAAAAGGCCACGAAGAGGTCAATCAAGGAGAGGGAGGCAGCAGAAAGACTTCCAGAGAGATGTACTTCCCAGTCTTGTTTCTTTGTCAAGAAATGAGGTGACTGATGATCTTCAAATAATTGAAGGGTTGATTATGGAATCTGGTGGGAGTTGGCTGTCAAGTTTGTCACAAAGAAATTCCGGTAAGGGTGGTAGGGGAAGGGGGAGGAAGCGTGTGGGAACTTCTGCTCCGTCTACTACTGTGGCTGAAGTTAACCAGCCCCAGATAGAGCAACCGAAATGCGTGGAGCTGCAGGGACCTGAGGAGAGAAATCTAACCTGTTGGGGTAAGAGGACAAGGCGTCCACCACGGCAAAGATATCCGATTGATAATTCTCCTATTCCCCTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

381

Amino Acids

42.43

Weight (kDa)

5.88

Isoelectric Point (pI)

65.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 114 - 371 2.1e-70 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 608
Acc36I ACCTGC 1 cut(s) 608
Acc65I GGTACC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 261, 352
AccI GTMKAC 1 cut(s) 983
AciI CCGC 3 cut(s) 59, 130, 596
AcsI RAATTY 3 cut(s) 670, 715, 925
AcuI CTGAAG 2 cut(s) 233, 1017
AcyI GRCGYC 1 cut(s) 1090
AdeI CACNNNGTG 1 cut(s) 163
AfaI GTAC 3 cut(s) 99, 263, 813
AfiI CCNNNNNNNGG 3 cut(s) 425, 543, 764
AgeI ACCGGT 1 cut(s) 355
AgsI TTSAA 7 cut(s) 14, 147, 299, 368, 398, 863, 872
AhdI GACNNNNNGTC 1 cut(s) 1089
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 2 cut(s) 274, 306
AloI GAACNNNNNNTCC 2 cut(s) 961, 993
AluBI AGCT 3 cut(s) 446, 458, 1039
AluI AGCT 3 cut(s) 446, 458, 1039
Alw21I GWGCWC 1 cut(s) 660
AlwNI CAGNNNCTG 2 cut(s) 256, 446
AoxI GGCC 4 cut(s) 87, 427, 695, 755
ApeKI GCWGC 5 cut(s) 440, 443, 458, 787, 1039
ApoI RAATTY 3 cut(s) 670, 715, 925
ArsI GACNNNNNNTTYG 2 cut(s) 655, 687
AsiGI ACCGGT 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 797
Asp718I GGTACC 1 cut(s) 261
AspS9I GGNCC 3 cut(s) 427, 696, 1046
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 7 cut(s) 101, 149, 332, 350, 408, 632, 859
AvaII GGWCC 1 cut(s) 1046
AxyI CCTNAGG 1 cut(s) 1050
BanI GGYRCC 2 cut(s) 261, 352
BbsI GAAGAC 2 cut(s) 409, 696
Bbv12I GWGCWC 1 cut(s) 660
BbvCI CCTCAGC 2 cut(s) 257, 447
BbvI GCAGC 5 cut(s) 445, 452, 455, 799, 1026
BccI CCATC 2 cut(s) 27, 470
BceAI ACGGC 1 cut(s) 1115
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 511
BcnI CCSGG 1 cut(s) 161
BfmI CTRYAG 2 cut(s) 615, 1040
BfuAI ACCTGC 1 cut(s) 608
BfuI GTATCC 1 cut(s) 511
BisI GCNGC 6 cut(s) 441, 444, 459, 597, 788, 1040
BlsI GCNGC 6 cut(s) 442, 445, 460, 598, 789, 1041
Bme1390I CCNGG 2 cut(s) 134, 161
Bme18I GGWCC 1 cut(s) 1046
BmeRI GACNNNNNGTC 1 cut(s) 1089
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 427, 696, 1046
BmiI GGNNCC 5 cut(s) 140, 263, 354, 453, 1047
BmrFI CCNGG 2 cut(s) 134, 161
BmrI ACTGGG 2 cut(s) 23, 813
BmsI GCATC 1 cut(s) 487
BmuI ACTGGG 2 cut(s) 23, 813
BoxI GACNNNNGTC 1 cut(s) 576
BpiI GAAGAC 2 cut(s) 409, 696
BpmI CTGGAG 1 cut(s) 438
Bpu10I CCTNAGC 2 cut(s) 257, 447
BpuMI CCSGG 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 1090
BsaJI CCNNGG 3 cut(s) 133, 684, 1097
BsaWI WCCGGW 2 cut(s) 355, 929
BsaXI ACNNNNNCTCC 4 cut(s) 946, 961, 976, 991
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 543, 764
Bse118I RCCGGY 2 cut(s) 355, 693
Bse1I ACTGG 3 cut(s) 29, 508, 819
Bse21I CCTNAGG 1 cut(s) 1050
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 133, 684, 1097
BseLI CCNNNNNNNGG 3 cut(s) 425, 543, 764
BseMII CTCAG 3 cut(s) 248, 438, 1041
BseNI ACTGG 3 cut(s) 29, 508, 819
BseRI GAGGAG 1 cut(s) 1067
BseXI GCAGC 5 cut(s) 445, 452, 455, 799, 1026
BshFI GGCC 4 cut(s) 89, 429, 697, 757
BshNI GGYRCC 2 cut(s) 261, 352
BshTI ACCGGT 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 660
BsiSI CCGG 4 cut(s) 160, 356, 694, 930
BslFI GGGAC 1 cut(s) 1059
BslI CCNNNNNNNGG 3 cut(s) 425, 543, 764
BsmFI GGGAC 1 cut(s) 1059
BsnI GGCC 4 cut(s) 89, 429, 697, 757
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 2 cut(s) 39, 856
BspACI CCGC 3 cut(s) 59, 130, 596
BspANI GGCC 4 cut(s) 89, 429, 697, 757
BspCNI CTCAG 3 cut(s) 249, 439, 1042
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 5 cut(s) 140, 263, 354, 453, 1047
BspMAI CTGCAG 2 cut(s) 619, 1044
BspMI ACCTGC 1 cut(s) 608
BspT107I GGYRCC 2 cut(s) 261, 352
BsrFI RCCGGY 2 cut(s) 355, 693
BsrI ACTGG 3 cut(s) 29, 508, 819
BssAI RCCGGY 2 cut(s) 355, 693
BssECI CCNNGG 3 cut(s) 133, 684, 1097
BssMI GATC 2 cut(s) 39, 856
BssNI GRCGYC 1 cut(s) 1090
BssT1I CCWWGG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 320, 991
Bst6I CTCTTC 3 cut(s) 252, 618, 757
BstACI GRCGYC 1 cut(s) 1090
BstDEI CTNAG 3 cut(s) 257, 447, 1050
BstDSI CCRYGG 1 cut(s) 1097
BstEII GGTNACC 1 cut(s) 155
BstKTI GATC 2 cut(s) 42, 859
BstMBI GATC 2 cut(s) 39, 856
BstMWI GCNNNNNNNGC 3 cut(s) 129, 449, 458
BstNI CCWGG 1 cut(s) 134
BstPAI GACNNNNGTC 1 cut(s) 576
BstPI GGTNACC 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 132, 159
BstSFI CTRYAG 2 cut(s) 615, 1040
BstV1I GCAGC 5 cut(s) 445, 452, 455, 799, 1026
BstV2I GAAGAC 2 cut(s) 409, 696
Bsu36I CCTNAGG 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 4 cut(s) 89, 429, 697, 757
BtgI CCRYGG 1 cut(s) 1097
BtrI CACGTC 1 cut(s) 407
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 309, 501
BveI ACCTGC 1 cut(s) 608
CaiI CAGNNNCTG 2 cut(s) 256, 446
CciI TCATGA 1 cut(s) 421
Cfr10I RCCGGY 2 cut(s) 355, 693
Cfr13I GGNCC 3 cut(s) 427, 696, 1046
CseI GACGC 1 cut(s) 1079
Csp6I GTAC 3 cut(s) 98, 262, 812
CspAI ACCGGT 1 cut(s) 355
CviAII CATG 4 cut(s) 215, 422, 567, 661
CviQI GTAC 3 cut(s) 98, 262, 812
DdeI CTNAG 3 cut(s) 257, 447, 1050
DpnI GATC 2 cut(s) 41, 858
DpnII GATC 2 cut(s) 39, 856
DraIII CACNNNGTG 1 cut(s) 163
DriI GACNNNNNGTC 1 cut(s) 1089
Eam1104I CTCTTC 3 cut(s) 252, 618, 757
Eam1105I GACNNNNNGTC 1 cut(s) 1089
EarI CTCTTC 3 cut(s) 252, 618, 757
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 684
Eco32I GATATC 1 cut(s) 1109
Eco47I GGWCC 1 cut(s) 1046
Eco57I CTGAAG 2 cut(s) 233, 1017
Eco81I CCTNAGG 1 cut(s) 1050
Eco91I GGTNACC 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 427, 1046
EcoO65I GGTNACC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 1109
EcoT14I CCWWGG 1 cut(s) 684
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 4 cut(s) 218, 425, 570, 664
FaqI GGGAC 1 cut(s) 1059
FatI CATG 4 cut(s) 214, 421, 566, 660
FblI GTMKAC 1 cut(s) 983
Fnu4HI GCNGC 6 cut(s) 441, 444, 459, 597, 788, 1040
Fsp4HI GCNGC 6 cut(s) 441, 444, 459, 597, 788, 1040
GluI GCNGC 6 cut(s) 441, 444, 459, 597, 788, 1040
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 4 cut(s) 89, 429, 697, 757
HapII CCGG 4 cut(s) 160, 356, 694, 930
HgaI GACGC 1 cut(s) 1079
Hin1I GRCGYC 1 cut(s) 1090
Hin1II CATG 4 cut(s) 218, 425, 570, 664
HincII GTYRAC 2 cut(s) 187, 1003
HindII GTYRAC 2 cut(s) 187, 1003
HinfI GANTC 6 cut(s) 323, 389, 413, 533, 626, 887
HpaI GTTAAC 1 cut(s) 1003
HpaII CCGG 4 cut(s) 160, 356, 694, 930
HphI GGTGA 7 cut(s) 101, 149, 332, 350, 408, 632, 859
Hpy166II GTNNAC 5 cut(s) 187, 230, 984, 1003, 1094
Hpy188I TCNGA 3 cut(s) 201, 721, 1113
Hpy188III TCNNGA 6 cut(s) 422, 482, 581, 635, 802, 837
Hpy8I GTNNAC 5 cut(s) 187, 230, 984, 1003, 1094
HpyAV CCTTC 4 cut(s) 329, 548, 866, 942
HpyCH4III ACNGT 2 cut(s) 320, 991
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 9 cut(s) 176, 214, 289, 315, 490, 526, 551, 617, 1042
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 449, 458
HpyF3I CTNAG 3 cut(s) 257, 447, 1050
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 1090
Hsp92II CATG 4 cut(s) 218, 425, 570, 664
KpnI GGTACC 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 1003
Kzo9I GATC 2 cut(s) 39, 856
LmnI GCTCC 3 cut(s) 457, 982, 1036
Lsp1109I GCAGC 5 cut(s) 445, 452, 455, 799, 1026
LweI GCATC 1 cut(s) 487
MaeII ACGT 1 cut(s) 406
MalI GATC 2 cut(s) 41, 858
MboI GATC 2 cut(s) 39, 856
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 8 cut(s) 9, 177, 558, 670, 715, 867, 925, 1120
MlyI GAGTC 3 cut(s) 407, 527, 635
MmeI TCCRAC 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 797
MseI TTAA 4 cut(s) 35, 606, 668, 1002
MspA1I CMGCKG 2 cut(s) 446, 458
MspI CCGG 4 cut(s) 160, 356, 694, 930
MspR9I CCNGG 2 cut(s) 134, 161
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 3 cut(s) 129, 449, 458
NciI CCSGG 1 cut(s) 161
NdeII GATC 2 cut(s) 39, 856
NlaIII CATG 4 cut(s) 218, 425, 570, 664
NlaIV GGNNCC 5 cut(s) 140, 263, 354, 453, 1047
NmuCI GTSAC 9 cut(s) 47, 107, 155, 306, 569, 640, 739, 847, 915
PagI TCATGA 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 797
PfeI GAWTC 3 cut(s) 323, 389, 887
PflFI GACNNNGTC 1 cut(s) 745
PinAI ACCGGT 1 cut(s) 355
PkrI GCNGC 6 cut(s) 442, 445, 460, 598, 789, 1041
PleI GAGTC 3 cut(s) 407, 527, 634
PpsI GAGTC 3 cut(s) 407, 527, 634
PpuMI RGGWCCY 1 cut(s) 1046
PshAI GACNNNNGTC 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 1046
Psp6I CCWGG 1 cut(s) 132
PspEI GGTNACC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 5 cut(s) 140, 263, 354, 453, 1047
PspPI GGNCC 3 cut(s) 427, 696, 1046
PspPPI RGGWCCY 1 cut(s) 1046
PstI CTGCAG 2 cut(s) 619, 1044
PstNI CAGNNNCTG 2 cut(s) 256, 446
PsyI GACNNNGTC 1 cut(s) 745
PvuII CAGCTG 2 cut(s) 446, 458
RsaI GTAC 3 cut(s) 99, 263, 813
RsaNI GTAC 3 cut(s) 98, 262, 812
SaqAI TTAA 4 cut(s) 35, 606, 668, 1002
SatI GCNGC 6 cut(s) 441, 444, 459, 597, 788, 1040
Sau3AI GATC 2 cut(s) 39, 856
Sau96I GGNCC 3 cut(s) 427, 696, 1046
SchI GAGTC 3 cut(s) 407, 527, 635
ScrFI CCNGG 2 cut(s) 134, 161
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 2 cut(s) 615, 1040
SinI GGWCC 1 cut(s) 1046
Sse9I AATT 8 cut(s) 9, 177, 558, 670, 715, 867, 925, 1120
SsiI CCGC 3 cut(s) 59, 130, 596
SspI AATATT 1 cut(s) 238
StyD4I CCNGG 2 cut(s) 132, 159
StyI CCWWGG 1 cut(s) 684
TaaI ACNGT 2 cut(s) 320, 991
TaiI ACGT 1 cut(s) 409
TasI AATT 8 cut(s) 9, 177, 558, 670, 715, 867, 925, 1120
TatI WGTACW 2 cut(s) 97, 811
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 3 cut(s) 323, 389, 887
Tru1I TTAA 4 cut(s) 35, 606, 668, 1002
Tru9I TTAA 4 cut(s) 35, 606, 668, 1002
TscAI CASTG 2 cut(s) 309, 508
TseFI GTSAC 9 cut(s) 47, 107, 155, 306, 569, 640, 739, 847, 915
TseI GCWGC 5 cut(s) 440, 443, 458, 787, 1039
Tsp45I GTSAC 9 cut(s) 47, 107, 155, 306, 569, 640, 739, 847, 915
TspDTI ATGAA 3 cut(s) 108, 219, 555
TspGWI ACGGA 3 cut(s) 588, 659, 969
TspRI CASTG 2 cut(s) 309, 508
Tth111I GACNNNGTC 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 1046
XapI RAATTY 3 cut(s) 670, 715, 925
XmiI GTMKAC 1 cut(s) 983
XmnI GAANNNNTTC 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.