pycom11g03570

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002116
Physical Location & Seq
Forward (+)
2766646 .. 2772595
5950 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGGGTGTTGGTGAAGCAGCAGAAGATCAGCAGTTACCGTCGCTCATAAGTTCTTCATCAGGTGAACTCAACCAACCTCTCAAAAGAACTGATAGGAACTCTTCCGACTGCAATTGCACATATAATAACATAACAGGTGTGATAGGAGCAGGAGTATTATCCCTTGCATGGAGTGTGGCTCAGCTAGGGTGGATTGGAGGTCCATTGCTTATGATTGTATTTGCTTCAGTCACCATTCTTTCCACCAACCTCCTCTCCGACTGCTACCGGTACCCCGAATCCAATAGCCCGATGAGGGTCGGATCCTACTTAGAAGCTGTTAAGGTCTACCTAGGAGAGAAGAGTCAGACAGTGTGTGCAATATTTGCACAGGAGAGCCTGTATGGCACCGGAATTGCCTACGTTATTACAGCTGCTGGCAGCGTCAAGCCTATATAG

Protein Analysis

146

Amino Acids

15.33

Weight (kDa)

4.76

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 143 1.1e-21 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 270
AccB1I GGYRCC 2 cut(s) 270, 386
AccI GTMKAC 1 cut(s) 327
AclWI GGATC 2 cut(s) 297, 310
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 168, 295
AgeI ACCGGT 1 cut(s) 267
AluBI AGCT 3 cut(s) 184, 317, 413
AluI AGCT 3 cut(s) 184, 317, 413
AlwI GGATC 2 cut(s) 297, 310
AlwNI CAGNNNCTG 1 cut(s) 416
ApeKI GCWGC 3 cut(s) 17, 413, 420
AsiGI ACCGGT 1 cut(s) 267
Asp718I GGTACC 1 cut(s) 270
AspA2I CCTAGG 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 23, 74, 223
AvaII GGWCC 1 cut(s) 200
AvrII CCTAGG 1 cut(s) 331
BamHI GGATCC 1 cut(s) 302
BanI GGYRCC 2 cut(s) 270, 386
BbvI GCAGC 3 cut(s) 29, 400, 432
BfaI CTAG 2 cut(s) 185, 332
BglI GCCNNNNNGGC 1 cut(s) 384
BisI GCNGC 3 cut(s) 18, 414, 421
BlnI CCTAGG 1 cut(s) 331
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 3 cut(s) 19, 415, 422
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 3 cut(s) 272, 304, 388
BplI GAGNNNNNCTC 2 cut(s) 163, 195
Bpu1102I GCTNAGC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 331
BsaWI WCCGGW 2 cut(s) 267, 389
BsaXI ACNNNNNCTCC 6 cut(s) 138, 168, 239, 269, 365, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 295
Bse118I RCCGGY 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 168, 295
BseMI GCAATG 1 cut(s) 203
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 3 cut(s) 29, 400, 432
BshNI GGYRCC 2 cut(s) 270, 386
BshTI ACCGGT 1 cut(s) 267
BsiSI CCGG 2 cut(s) 268, 390
BslI CCNNNNNNNGG 2 cut(s) 168, 295
Bsp143I GATC 2 cut(s) 25, 302
Bsp1720I GCTNAGC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 3 cut(s) 272, 304, 388
BspPI GGATC 2 cut(s) 297, 310
BspT107I GGYRCC 2 cut(s) 270, 386
BsrDI GCAATG 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 331
BssMI GATC 2 cut(s) 25, 302
BssT1I CCWWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 39, 352
Bst6I CTCTTC 2 cut(s) 106, 335
BstAPI GCANNNNNTGC 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 2 cut(s) 180, 310
BstKTI GATC 2 cut(s) 28, 305
BstMBI GATC 2 cut(s) 25, 302
BstMWI GCNNNNNNNGC 2 cut(s) 365, 384
BstV1I GCAGC 3 cut(s) 29, 400, 432
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 418
CaiI CAGNNNCTG 1 cut(s) 416
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 200
CseI GACGC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 271
CspAI ACCGGT 1 cut(s) 267
CviAII CATG 1 cut(s) 168
CviJI RGCY 7 cut(s) 179, 184, 288, 317, 378, 413, 430
CviKI_1 RGCY 7 cut(s) 179, 184, 288, 317, 378, 413, 430
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 2 cut(s) 180, 310
DpnI GATC 2 cut(s) 27, 304
DpnII GATC 2 cut(s) 25, 302
Eam1104I CTCTTC 2 cut(s) 106, 335
EarI CTCTTC 2 cut(s) 106, 335
Eco130I CCWWGG 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 171
FaiI YATR 9 cut(s) 47, 121, 123, 131, 169, 212, 384, 434, 436
FatI CATG 1 cut(s) 167
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 18, 414, 421
Fsp4HI GCNGC 3 cut(s) 18, 414, 421
FspBI CTAG 2 cut(s) 185, 332
GluI GCNGC 3 cut(s) 18, 414, 421
HapII CCGG 2 cut(s) 268, 390
HgaI GACGC 1 cut(s) 412
Hin1II CATG 1 cut(s) 171
HinfI GANTC 2 cut(s) 278, 343
HpaII CCGG 2 cut(s) 268, 390
HphI GGTGA 3 cut(s) 23, 74, 223
Hpy166II GTNNAC 2 cut(s) 65, 328
Hpy188I TCNGA 4 cut(s) 106, 259, 302, 348
Hpy8I GTNNAC 2 cut(s) 65, 328
Hpy99I CGWCG 1 cut(s) 43
HpyCH4III ACNGT 2 cut(s) 39, 352
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 111, 117, 167, 359, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 384
HpyF3I CTNAG 2 cut(s) 180, 310
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 171
KpnI GGTACC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 25, 302
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 8 cut(s) 45, 120, 135, 281, 356, 392, 402, 403
Lsp1109I GCAGC 3 cut(s) 29, 400, 432
MaeI CTAG 2 cut(s) 185, 332
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 33, 229
MalI GATC 2 cut(s) 27, 304
MboI GATC 2 cut(s) 25, 302
MboII GAAGA 4 cut(s) 35, 45, 93, 352
MfeI CAATTG 1 cut(s) 112
MflI RGATCY 1 cut(s) 302
MluCI AATT 2 cut(s) 112, 393
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 3 cut(s) 129, 280, 282
MnlI CCTC 5 cut(s) 87, 191, 260, 263, 288
MseI TTAA 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 2 cut(s) 268, 390
MunI CAATTG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 365, 384
NdeII GATC 2 cut(s) 25, 302
NlaIII CATG 1 cut(s) 171
NlaIV GGNNCC 3 cut(s) 272, 304, 388
NmuCI GTSAC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 267
PkrI GCNGC 3 cut(s) 19, 415, 422
PleI GAGTC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 272, 304, 388
PspPI GGNCC 1 cut(s) 200
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 18, 414, 421
Sau3AI GATC 2 cut(s) 25, 302
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 352
SinI GGWCC 1 cut(s) 200
Sse9I AATT 2 cut(s) 112, 393
SspI AATATT 1 cut(s) 363
SspMI CTAG 2 cut(s) 185, 332
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 39, 352
TaiI ACGT 1 cut(s) 405
TasI AATT 2 cut(s) 112, 393
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 3 cut(s) 17, 413, 420
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 45
TspRI CASTG 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 200
XmaJI CCTAGG 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 2 cut(s) 185, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.