pycom11g03580

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002116
Physical Location & Seq
Forward (+)
2772675 .. 2775191
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1383 bp
ATGGCAATTCAACACCCACTTGAGTCAGCTAGTAATGGTTCCTGTGACGATGATGGCCATCCACTAAGAACTGGAACGCTGTGGAGTTGTGTGGCTCATATCATCACTGCTGTGATTGGATCTGGAGTTTTGTCTTTGGCATGGAGTACTGCTCACCTAGGGTGGATTGGAGGCCCCGTTTCGTTGCTTTGTTTTGCAATTGTCACCTACATTTCTTCCTTCCTCGTTTCGGATTGTTACCGGTCTCCTGATCCTATCACCGGAACTCGAAACAAATCTTACATGGATGCTGTGAAAGTGAATCTCAATAGTAGAAAAAAGACCTGGTTTTGTGGTTTACTTCTGTATTTCAGCATGTTTGGAACTGGTATTGCTTATGTGATTACCACTGCGACCAGTATGAGAGCCATTCAGAGATCAAACTGCTACCACAAAGAAGGGCATGAGGCTTCATGTGAATATGGAACTAGTCTGTATATGCTGCTATTTGGACTTGTCCAGATTGTTGCGTCACAAATACCAGATTTCCACAACATGGAATGGCTTTCAGTTATCGCAGCAATCATGTCCTTTGCCTACTCTTTCATTGGACTTGGCCTCGGCTTTGCAAAAGTAATAGAAAATGGGAAGATTCAAGGAAGCCTAACAGGAGTTAGAACATCAAATGTTGCTGATAAATTATGGTTATCCTTCCAAGCACTTGGAGACATTGCTTTTGCCTATCCATACTCTATTATTGTTCTTGAAGTTCAGGATACTTTGAAGTCCCCTCCACCAGAAAATCAGACAATGAAGAAGGCCTCAATGATTGCCATATTTGTCACAACCTTCTTCTACCTCTGCTGCGGATGCTTTGGATACGCCGCCTTTGGCGATGACACCCCAGGGAATCTCTTGACAGGATTCGGATTCTACGAACCCTTCTGGCTCATTGATTTTGCCAATGCTTGCATTGCTCTTCATTTGGTGGGAGGATATCAGGTCTACAGTCAACCAGTATTTGCAGTTGCTGAAAGATGGTCCACGAAGAAATACCCGAGCAGTGGATTTGTGAACAACTTTTACACTATCAAGGTCCCATTGTTACCGAGATTTCAAGTGAATCCCTTCAGGCTGTGTTTCCGAACTGTTTACGTTGTGTCAACTACCGCAATTGCGATGATATTTCCTTACTTCAACCAGGTTTTGGGAGTTTTAGGGGCCTTGAACTTTTGGCCTTTGACCATATATTTTCCTGTAGAAATGTACTTTGTACAGAAGAAAATTGGGGCTTGGACAAAAACATGGGTTGTTCTGAGGACATTTAGTTTAGCTTGCTTTCTTGTAACAGTTGTGGGTGTGATTGGATCAGTTGAAGGACTTATAAGTGCCAAACTGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

461

Amino Acids

50.78

Weight (kDa)

8.46

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 24 - 455 1.4e-100 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1364
AccB7I CCANNNNNTGG 2 cut(s) 535, 1186
AccI GTMKAC 1 cut(s) 984
AciI CCGC 3 cut(s) 846, 864, 1149
AclWI GGATC 3 cut(s) 127, 245, 1354
AcoI YGGCCR 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 1093
AfaI GTAC 3 cut(s) 148, 1247, 1254
AfiI CCNNNNNNNGG 5 cut(s) 229, 260, 535, 1043, 1186
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 8 cut(s) 11, 635, 746, 763, 1097, 1177, 1207, 1355
AhlI ACTAGT 1 cut(s) 467
AjnI CCWGG 3 cut(s) 323, 883, 1179
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 3 cut(s) 29, 1313, 1380
AluI AGCT 3 cut(s) 29, 1313, 1380
Alw26I GTCTC 2 cut(s) 249, 699
AlwI GGATC 3 cut(s) 127, 245, 1354
AlwNI CAGNNNCTG 1 cut(s) 1010
Ama87I CYCGRG 1 cut(s) 1036
AoxI GGCC 6 cut(s) 55, 172, 595, 798, 1200, 1214
ApeKI GCWGC 3 cut(s) 481, 557, 843
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AsiGI ACCGGT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 1106
AspA2I CCTAGG 1 cut(s) 157
AspS9I GGNCC 4 cut(s) 173, 1020, 1075, 1200
AsuHPI GGTGA 3 cut(s) 146, 196, 250
AvaI CYCGRG 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 1020, 1075
AvrII CCTAGG 1 cut(s) 157
BalI TGGCCA 1 cut(s) 57
BbvI GCAGC 3 cut(s) 468, 569, 830
BccI CCATC 3 cut(s) 47, 66, 1011
BciT130I CCWGG 3 cut(s) 325, 885, 1181
BciVI GTATCC 2 cut(s) 748, 851
BcoDI GTCTC 2 cut(s) 249, 699
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 4 cut(s) 30, 158, 468, 1381
BfmI CTRYAG 2 cut(s) 985, 1236
BfuI GTATCC 2 cut(s) 748, 851
BisI GCNGC 4 cut(s) 482, 558, 844, 864
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 4 cut(s) 483, 559, 845, 865
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 325, 885, 1181
Bme18I GGWCC 2 cut(s) 1020, 1075
BmeT110I CYCGRG 1 cut(s) 1036
BmgT120I GGNCC 4 cut(s) 173, 1020, 1075, 1200
BmiI GGNNCC 4 cut(s) 40, 175, 1077, 1201
BmrFI CCNGG 3 cut(s) 325, 885, 1181
BmsI GCATC 2 cut(s) 277, 839
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 157, 598, 883, 884
BsaWI WCCGGW 2 cut(s) 240, 260
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 260, 535, 1043, 1186
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 4 cut(s) 76, 370, 396, 995
Bse3DI GCAATG 2 cut(s) 708, 951
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 3 cut(s) 325, 885, 1181
BseDI CCNNGG 4 cut(s) 157, 598, 883, 884
BseGI GGATG 3 cut(s) 58, 292, 854
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 5 cut(s) 229, 260, 535, 1043, 1186
BseMI GCAATG 2 cut(s) 708, 951
BseMII CTCAG 2 cut(s) 1286, 1367
BseNI ACTGG 4 cut(s) 76, 370, 396, 995
BseXI GCAGC 3 cut(s) 468, 569, 830
BshFI GGCC 6 cut(s) 57, 174, 597, 800, 1202, 1216
BshTI ACCGGT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 1036
BsiSI CCGG 2 cut(s) 241, 261
BslFI GGGAC 2 cut(s) 751, 1061
BslI CCNNNNNNNGG 5 cut(s) 229, 260, 535, 1043, 1186
BsmAI GTCTC 2 cut(s) 249, 699
BsmFI GGGAC 2 cut(s) 751, 1061
BsnI GGCC 6 cut(s) 57, 174, 597, 800, 1202, 1216
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 1036
Bsp1407I TGTACA 1 cut(s) 1252
Bsp143I GATC 4 cut(s) 119, 250, 416, 1346
BspACI CCGC 3 cut(s) 846, 864, 1149
BspANI GGCC 6 cut(s) 57, 174, 597, 800, 1202, 1216
BspCNI CTCAG 2 cut(s) 1287, 1368
BspLI GGNNCC 4 cut(s) 40, 175, 1077, 1201
BspPI GGATC 3 cut(s) 127, 245, 1354
BspQI GCTCTTC 1 cut(s) 963
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 2 cut(s) 708, 951
BsrFI RCCGGY 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 1252
BsrI ACTGG 4 cut(s) 76, 370, 396, 995
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 4 cut(s) 157, 598, 883, 884
BssMI GATC 4 cut(s) 119, 250, 416, 1346
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 3 cut(s) 325, 885, 1181
Bst4CI ACNGT 3 cut(s) 989, 1129, 1330
Bst6I CTCTTC 1 cut(s) 963
BstAUI TGTACA 1 cut(s) 1252
BstC8I GCNNGC 2 cut(s) 949, 1315
BstDEI CTNAG 3 cut(s) 65, 1295, 1376
BstF5I GGATG 3 cut(s) 58, 292, 854
BstKTI GATC 4 cut(s) 122, 253, 419, 1349
BstMAI GTCTC 2 cut(s) 249, 699
BstMBI GATC 4 cut(s) 119, 250, 416, 1346
BstMWI GCNNNNNNNGC 2 cut(s) 849, 953
BstNI CCWGG 3 cut(s) 325, 885, 1181
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 3 cut(s) 323, 883, 1179
BstSFI CTRYAG 2 cut(s) 985, 1236
BstV1I GCAGC 3 cut(s) 468, 569, 830
BstX2I RGATCY 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 701
BstYI RGATCY 1 cut(s) 119
BsuI GTATCC 2 cut(s) 748, 851
BsuRI GGCC 6 cut(s) 57, 174, 597, 800, 1202, 1216
BtgZI GCGATG 2 cut(s) 888, 1172
BtsCI GGATG 3 cut(s) 58, 292, 854
BtsI GCAGTG 3 cut(s) 105, 387, 1048
BtsIMutI CAGTG 3 cut(s) 105, 387, 1048
Cac8I GCNNGC 2 cut(s) 949, 1315
CaiI CAGNNNCTG 1 cut(s) 1010
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 4 cut(s) 173, 1020, 1075, 1200
CseI GACGC 1 cut(s) 498
CsiI ACCWGGT 2 cut(s) 323, 1179
Csp6I GTAC 3 cut(s) 147, 1246, 1253
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 8 cut(s) 141, 283, 355, 443, 453, 535, 565, 1284
CviQI GTAC 3 cut(s) 147, 1246, 1253
DdeI CTNAG 3 cut(s) 65, 1295, 1376
DpnI GATC 4 cut(s) 121, 252, 418, 1348
DpnII GATC 4 cut(s) 119, 250, 416, 1346
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Eco130I CCWWGG 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 800
Eco31I GGTCTC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 977
Eco47I GGWCC 2 cut(s) 1020, 1075
Eco57I CTGAAG 1 cut(s) 1093
Eco88I CYCGRG 1 cut(s) 1036
EcoO109I RGGNCCY 3 cut(s) 173, 1075, 1200
EcoRII CCWGG 3 cut(s) 323, 883, 1179
EcoRV GATATC 1 cut(s) 977
EcoT14I CCWWGG 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 8 cut(s) 144, 286, 358, 446, 456, 538, 568, 1287
FaqI GGGAC 2 cut(s) 751, 1061
FatI CATG 8 cut(s) 140, 282, 354, 442, 452, 534, 564, 1283
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 4 cut(s) 482, 558, 844, 864
FokI GGATG 3 cut(s) 45, 299, 861
Fsp4HI GCNGC 4 cut(s) 482, 558, 844, 864
FspBI CTAG 4 cut(s) 30, 158, 468, 1381
GluI GCNGC 4 cut(s) 482, 558, 844, 864
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 6 cut(s) 57, 174, 597, 800, 1202, 1216
HapII CCGG 2 cut(s) 241, 261
HgaI GACGC 1 cut(s) 498
Hin1II CATG 8 cut(s) 144, 286, 358, 446, 456, 538, 568, 1287
HincII GTYRAC 2 cut(s) 992, 1143
HindII GTYRAC 2 cut(s) 992, 1143
HinfI GANTC 7 cut(s) 23, 301, 631, 889, 903, 909, 1102
HpaII CCGG 2 cut(s) 241, 261
HphI GGTGA 3 cut(s) 146, 196, 250
Hpy166II GTNNAC 7 cut(s) 338, 985, 992, 1023, 1054, 1132, 1143
Hpy188I TCNGA 6 cut(s) 232, 414, 786, 908, 1124, 1296
Hpy188III TCNNGA 6 cut(s) 123, 248, 499, 743, 752, 895
Hpy8I GTNNAC 7 cut(s) 338, 985, 992, 1023, 1054, 1132, 1143
HpyAV CCTTC 8 cut(s) 229, 431, 700, 790, 838, 931, 1117, 1349
HpyCH4III ACNGT 3 cut(s) 989, 1129, 1330
HpyCH4IV ACGT 1 cut(s) 1134
HpyCH4V TGCA 4 cut(s) 197, 608, 951, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 849, 953
HpyF3I CTNAG 3 cut(s) 65, 1295, 1376
HpySE526I ACGT 1 cut(s) 1134
Hsp92II CATG 8 cut(s) 144, 286, 358, 446, 456, 538, 568, 1287
Kzo9I GATC 4 cut(s) 119, 250, 416, 1346
LguI GCTCTTC 1 cut(s) 963
Lsp1109I GCAGC 3 cut(s) 468, 569, 830
LweI GCATC 2 cut(s) 277, 839
MabI ACCWGGT 2 cut(s) 323, 1179
MaeI CTAG 4 cut(s) 30, 158, 468, 1381
MaeII ACGT 1 cut(s) 1134
MaeIII GTNAC 7 cut(s) 44, 202, 236, 510, 820, 1083, 1324
MalI GATC 4 cut(s) 121, 252, 418, 1348
MboI GATC 4 cut(s) 119, 250, 416, 1346
MboII GAAGA 7 cut(s) 207, 640, 805, 823, 950, 1039, 1270
MfeI CAATTG 2 cut(s) 198, 1152
MflI RGATCY 1 cut(s) 119
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 5 cut(s) 6, 198, 677, 1152, 1263
MluNI TGGCCA 1 cut(s) 57
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 9 cut(s) 164, 233, 439, 608, 780, 811, 848, 965, 1290
Mox20I TGGCCA 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 1106
MscI TGGCCA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 57
MspI CCGG 2 cut(s) 241, 261
MspR9I CCNGG 3 cut(s) 325, 885, 1181
MunI CAATTG 2 cut(s) 198, 1152
MvaI CCWGG 3 cut(s) 325, 885, 1181
MwoI GCNNNNNNNGC 2 cut(s) 849, 953
NdeII GATC 4 cut(s) 119, 250, 416, 1346
NlaIII CATG 8 cut(s) 144, 286, 358, 446, 456, 538, 568, 1287
NlaIV GGNNCC 4 cut(s) 40, 175, 1077, 1201
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 4 cut(s) 44, 202, 510, 820
NspI RCATGY 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 110
PasI CCCWGGG 1 cut(s) 884
PceI AGGCCT 1 cut(s) 800
PciSI GCTCTTC 1 cut(s) 963
PcsI WCGNNNNNNNCGW 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 6 cut(s) 301, 631, 889, 903, 909, 1102
PflMI CCANNNNNTGG 2 cut(s) 535, 1186
PinAI ACCGGT 1 cut(s) 240
PkrI GCNGC 4 cut(s) 483, 559, 845, 865
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 1075
PsiI TTATAA 1 cut(s) 1364
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 3 cut(s) 323, 883, 1179
PspGI CCWGG 3 cut(s) 323, 883, 1179
PspN4I GGNNCC 4 cut(s) 40, 175, 1077, 1201
PspPI GGNCC 4 cut(s) 173, 1020, 1075, 1200
PspPPI RGGWCCY 1 cut(s) 1075
PsrI GAACNNNNNNTAC 2 cut(s) 1046, 1078
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 3 cut(s) 148, 1247, 1254
RsaNI GTAC 3 cut(s) 147, 1246, 1253
RseI CAYNNNNRTG 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 963
SatI GCNGC 4 cut(s) 482, 558, 844, 864
Sau3AI GATC 4 cut(s) 119, 250, 416, 1346
Sau96I GGNCC 4 cut(s) 173, 1020, 1075, 1200
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 3 cut(s) 325, 885, 1181
SexAI ACCWGGT 2 cut(s) 323, 1179
SfaNI GCATC 2 cut(s) 277, 839
SfcI CTRYAG 2 cut(s) 985, 1236
SinI GGWCC 2 cut(s) 1020, 1075
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 5 cut(s) 6, 198, 677, 1152, 1263
SseBI AGGCCT 1 cut(s) 800
SsiI CCGC 3 cut(s) 846, 864, 1149
SspMI CTAG 4 cut(s) 30, 158, 468, 1381
StuI AGGCCT 1 cut(s) 800
StyD4I CCNGG 3 cut(s) 323, 883, 1179
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 3 cut(s) 989, 1129, 1330
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 1 cut(s) 268
TasI AATT 5 cut(s) 6, 198, 677, 1152, 1263
TatI WGTACW 3 cut(s) 146, 1245, 1252
TauI GCSGC 1 cut(s) 866
TfiI GAWTC 6 cut(s) 301, 631, 889, 903, 909, 1102
TscAI CASTG 3 cut(s) 112, 394, 1048
TseFI GTSAC 4 cut(s) 44, 202, 510, 820
TseI GCWGC 3 cut(s) 481, 557, 843
Tsp45I GTSAC 4 cut(s) 44, 202, 510, 820
TspDTI ATGAA 4 cut(s) 441, 574, 806, 950
TspRI CASTG 3 cut(s) 112, 394, 1048
Van91I CCANNNNNTGG 2 cut(s) 535, 1186
VpaK11BI GGWCC 2 cut(s) 1020, 1075
XceI RCATGY 1 cut(s) 358
XmaJI CCTAGG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 4 cut(s) 30, 158, 468, 1381
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.