pycom11g06010

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005186
Physical Location & Seq
Forward (+)
4629849 .. 4634176
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3129 bp
ATGACGTCGTCGTCGTCGTCGTCCTCCGTCTCGTCGGCACTAGACTCAATCAACACCTCGCTCGCCGAGCTCTGCGCCGATCACTACCGCCACTCTCCCTTCGACCTCCCCCGCCGCTTCTCCGGCTTTGCTGACCGCCTTCAGCTGTCACTCACCCACCTCACCCGCGCCACGCCATCGCTCGACTCCCTCCCTCCCTCCGCCCACACCGCCCTCAAGGGCATCGCCGCCGACCTCGCCTCCGCACTCGAAACTCTGTCGTTCTACAGAACCAAGGGCAAAATCTCCGTCCTCATCAACTGCCTCTCCCTCTGCTCCTCCCTCCGCGACCGCACAGTCGCCATCTCCGGCTGGCTCGCCCTCCTCGACGCTGCGGTTCAGGACCTCAACCTCTCCGATCTTCGCAAGAAGATCGCCGATCTCTCCAGAGACATGAAGCAAGCTCACTTCACAGTTTCGGAGAAGGAAGAACGAGTTCATCACATTCTGCAGAAAGAAGGATTAGCAACTCAATCGAAGACCAGCAAAGCAGTGGAGAGCGCGATAATCATGGACTTAGCCCGAGGGCTAGGGATCGACGCTGAGAATCATGACGAGCTCTCGAAGCAGATCATGCTTCTCAAAAACGACGTCGCAGGGTCCAATTTGGAGTCGGAACGGCGGATTCTGTGGTCGTTGGAGAGGATTCTGAACAACTGGGCCGTGCAGCGTCCCAGTTTCTCGGCTTGGAAGAAGGGAATGGAGTTCGAAGACGACGTTCACATTCAGCCGTTTAAGAACTTTCTCTGTCCGCTGACGAAGGAGGTGATGCGGGACCCGGTAGTGCTCCAGTCGTCGCAGACGTACGAGCGGACCGCCATTAATTACTGGTTCGAGCGGTGTTTGGAAGACGGGCGTGACCCGACTTGTCCGGTCACGGGTCAAGTTCTCGAGTCGCTGGAGGTGAAGCCGAACATTGGATTGTCCGGAGCTATAGAGGAGTGGGTCAATAGGAATGTTGATATTGTGGTGAAAATTTCAGCTCAGCATCTGAGTAAAGAGCCTCCGGCGGTGGATTGTGTGGAGGGAGTGCTGGATAATGTGTATAACATCTCGGAGGAGTATCCGAATTGTCGGTATAGAGTTAGGAATGGTGGCATTCTTGTTCTTATTGTTAAGATGCTCAGGAACAGTTCCAAGAGTATCGGGACATATTTGAGGAGCAAAGCTCTAATGGTTTTGCTTAGCATGGCCAAGGATGAAGAAAGCAAGAAAATCATGCTTCAAGAGGGTATCACTAGATTGGCCATACATAGTCTTACCGGGAGCTCGGAGAGAGAGAGAGAGTATGCTGTGAAACTTTTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGCGCGCTGGTTCTTCTCTCAAGTATGGCAGGAAACCTCGAGCATCCTGGTTTATCCAAATTGGCTGAGGAGGTACTAAAGTGGATGGAGAAGGTGGAGGTTAATGTTCAGCATTTGGCTGCGGCAGGGAGATTCGAACCATTGCTTACTCGACTTTGTGAAGGTTCCGATGGTGCCAAAATTGAAATGGCATCTTTGGTGGGAAGTATGACCTTGACAAATAGCAGCAAGGAACAAATAGCTAGGCGAAGTGCTAAAATACTGGTTGAGATGCTATCTAAGCCAGAAGGAAGAGCAGCAAGCTTGCAAGCGTTGTACAACTTGTCAAGTCTAGATGACAATGCAACCATCCTCGTTGATTCTGCTGTACTTCCCTCTCTTACAGCTATCCTTTTTATAAACCAGGATACTTCACCAGAACTCAAAGAATTGGCTGCAGCAACAATTGCAAATATAGTGTCAAATTCAGGGCACTGGGAATTGGCATATGCTGACAAGGAAGGGCATTCCATGCAGTCAGAAGCATTCATTTATGGTCTTTTGGGATCTTTACCACTTGCATCTCCTCAATGTCAAATTTCTATTCTCCACATCCTATATGGAATTGCATCGTCTCCACAGGCATCACAGTCGGTGGCTCGTCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAACACCCAGAAGTTGAACACAGGATTCATGCTTTTAGGCTTACAAGACTACTCTCAGAAAGGTGTGGTGAAGATATAGCTAACGAGCTAAGGCTTTCGAACAGGATTCCCCTGTTACAAGACAAACTATTAGACCACCAATCAATAGATAGTGAGAGATCTGATGCCGCATGCATACTTGCGAACCTTTCTCTTTCAGAAAATGAAGTGAAAACACTTCTTGGAGTTAGTTTTGTAAATTGGACAGTTACTACTCTTAAAAGCCAGCGCCAAGCTTCCAGCGGGAGGATTTCACGACCTGCATCAAACTTGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAGGAAACCTGACCGGCAAACTCTTGGCACATTCAAAGAGCATAGCCTTATTACTATTTTCCACGATCATCTTATCTATCCGTCAAACCCAAGAGTGAGACAACTAGCTGCCCTTGGATTGAAAAACTTGTCGGAATATGGAGGGTTCCTAGCTGCTGCGGAATCAGAACCACGACCTCCCCATGGATTCTTTTCGTATTTGGTCTTCATGTGTGGAAGCTCTTCTGTAGAACTACCAAGGTGTCCAATACACAATGTTTCATGTGAAGAAGACAGTCAGTTGTGCTTGCTAAAGAGCAACTCCATCACAACCCTCGTTGATCTACTGAACGACGAGGACACCAGTGTTCGGATTGCTGCAGTGGAGGCACTGTCCACTCTTGTAATAGAAACTTCTAGCAGCTTCAAAAGAGCAGTTGACCAGCTCGAAGATTTGGGTGTGATTGAAGCTGTGATTAACCTTTTCAGGGCAGTTCGACCGGGCGAGCTTCAGGAGCGAACAACTTGGATCATAGAGAAAGTGTTGAGAGTCGAAAACCATAGACATTCACTAAACCAGGCTCTGGTTTGGGCATTGGTGGAAGCCTTCAAGCACGGCAATTCGAACACCAAGAGACAGGCTCAGGATGCTCTTACCCATCTGAAGCAGTTATCCGCGGTTAGTGGAATATCGTCGCGTCAAAGTCGGACACATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1043

Amino Acids

115.06

Weight (kDa)

6.23

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 260 - 332 9.6e-16 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1793
AasI GACNNNNNNGTC 2 cut(s) 10, 335
AatII GACGTC 2 cut(s) 8, 633
Acc36I ACCTGC 1 cut(s) 2389
AccB1I GGYRCC 1 cut(s) 1568
AccB7I CCANNNNNTGG 1 cut(s) 2992
AccBSI CCGCTC 2 cut(s) 850, 877
AccII CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
AccIII TCCGGA 1 cut(s) 965
AclWI GGATC 3 cut(s) 581, 1948, 2945
AcoI YGGCCR 2 cut(s) 1230, 1284
AcsI RAATTY 3 cut(s) 1014, 1858, 1971
AcuI CTGAAG 3 cut(s) 125, 2903, 3092
AcyI GRCGYC 2 cut(s) 5, 630
AfaI GTAC 4 cut(s) 845, 1470, 1712, 1764
AfiI CCNNNNNNNGG 7 cut(s) 917, 956, 2367, 2612, 2778, 2896, 2992
AgsI TTSAA 9 cut(s) 1265, 1580, 2084, 2099, 2464, 2551, 2836, 2876, 3019
AjnI CCWGG 3 cut(s) 1441, 1797, 2985
AjuI GAANNNNNNNTTGG 2 cut(s) 2391, 2423
Alw21I GWGCWC 4 cut(s) 72, 600, 828, 1310
Alw26I GTCTC 5 cut(s) 34, 423, 2013, 2521, 3037
AlwI GGATC 3 cut(s) 581, 1948, 2945
AlwNI CAGNNNCTG 2 cut(s) 1030, 2992
Ama87I CYCGRG 3 cut(s) 561, 929, 1433
Aor13HI TCCGGA 1 cut(s) 965
AoxI GGCC 3 cut(s) 699, 1230, 1284
ApoI RAATTY 3 cut(s) 1014, 1858, 1971
ArsI GACNNNNNNTTYG 6 cut(s) 273, 305, 615, 647, 1838, 1870
AseI ATTAAT 1 cut(s) 861
Asp700I GAANNNNTTC 3 cut(s) 474, 2403, 2650
AspLEI GCGC 6 cut(s) 77, 170, 542, 1400, 1402, 2352
AspS9I GGNCC 5 cut(s) 382, 639, 699, 814, 852
AsuC2I CCSGG 3 cut(s) 818, 1303, 2910
AsuHPI GGTGA 7 cut(s) 145, 154, 817, 955, 1021, 1800, 2162
AsuII TTCGAA 4 cut(s) 747, 1530, 2180, 3032
AvaI CYCGRG 3 cut(s) 561, 929, 1433
AvaII GGWCC 4 cut(s) 382, 639, 814, 852
BaeGI GKGCMC 1 cut(s) 1869
BaeI ACNNNNGTAYC 2 cut(s) 1460, 1493
BalI TGGCCA 2 cut(s) 1232, 1286
BanI GGYRCC 1 cut(s) 1568
BanII GRGCYC 3 cut(s) 72, 600, 1310
BarI GAAGNNNNNNTAC 2 cut(s) 2618, 2650
BbsI GAAGAC 6 cut(s) 524, 756, 894, 2396, 2626, 2706
Bbv12I GWGCWC 4 cut(s) 72, 600, 828, 1310
BbvCI CCTCAGC 1 cut(s) 1461
BccI CCATC 7 cut(s) 184, 350, 1474, 1559, 1751, 2741, 3075
BceAI ACGGC 4 cut(s) 674, 686, 754, 3040
BcgI CGANNNNNNTGC 6 cut(s) 394, 428, 495, 529, 2007, 2041
BciT130I CCWGG 3 cut(s) 1443, 1799, 2987
BciVI GTATCC 2 cut(s) 1113, 1795
BcnI CCSGG 3 cut(s) 818, 1303, 2910
BcoDI GTCTC 5 cut(s) 34, 423, 2013, 2521, 3037
BfaI CTAG 9 cut(s) 41, 569, 1278, 1356, 1638, 1727, 2534, 2579, 2826
BfmI CTRYAG 6 cut(s) 265, 488, 972, 1830, 2655, 2787
BfoI RGCGCY 1 cut(s) 2353
BfuAI ACCTGC 1 cut(s) 2389
BfuI GTATCC 2 cut(s) 1113, 1795
BglII AGATCT 1 cut(s) 2240
BlpI GCTNAGC 2 cut(s) 1023, 1223
Bme1390I CCNGG 6 cut(s) 818, 1303, 1443, 1799, 2910, 2987
Bme18I GGWCC 4 cut(s) 382, 639, 814, 852
BmeT110I CYCGRG 3 cut(s) 561, 929, 1433
BmgT120I GGNCC 5 cut(s) 382, 639, 699, 814, 852
BmiI GGNNCC 6 cut(s) 640, 815, 816, 1561, 1570, 2576
BmrFI CCNGG 6 cut(s) 818, 1303, 1443, 1799, 2910, 2987
BmrI ACTGGG 3 cut(s) 706, 708, 1879
BmuI ACTGGG 3 cut(s) 706, 708, 1879
BpiI GAAGAC 6 cut(s) 524, 756, 894, 2396, 2626, 2706
BplI GAGNNNNNCTC 4 cut(s) 1192, 1224, 3034, 3066
BpmI CTGGAG 3 cut(s) 409, 812, 959
Bpu10I CCTNAGC 4 cut(s) 1163, 1461, 2171, 3051
Bpu1102I GCTNAGC 2 cut(s) 1023, 1223
Bpu14I TTCGAA 4 cut(s) 747, 1530, 2180, 3032
BpuEI CTTGAG 3 cut(s) 200, 1367, 1399
BpuMI CCSGG 3 cut(s) 818, 1303, 2910
BsaHI GRCGYC 2 cut(s) 5, 630
BsaJI CCNNGG 7 cut(s) 273, 562, 1233, 2542, 2611, 2666, 3084
BsaWI WCCGGW 2 cut(s) 910, 965
BsaXI ACNNNNNCTCC 6 cut(s) 224, 254, 290, 320, 2786, 2816
Bsc4I CCNNNNNNNGG 7 cut(s) 917, 956, 2367, 2612, 2778, 2896, 2992
Bse118I RCCGGY 1 cut(s) 2442
Bse1I ACTGG 7 cut(s) 701, 714, 829, 872, 1662, 1874, 2772
Bse3DI GCAATG 1 cut(s) 1535
BseAI TCCGGA 1 cut(s) 965
BseBI CCWGG 3 cut(s) 1443, 1799, 2987
BseDI CCNNGG 7 cut(s) 273, 562, 1233, 2542, 2611, 2666, 3084
BseGI GGATG 6 cut(s) 1243, 1438, 1485, 1743, 1986, 3061
BseLI CCNNNNNNNGG 7 cut(s) 917, 956, 2367, 2612, 2778, 2896, 2992
BseMI GCAATG 1 cut(s) 1535
BseMII CTCAG 7 cut(s) 573, 1022, 1037, 1177, 1452, 2151, 3065
BseNI ACTGG 7 cut(s) 701, 714, 829, 872, 1662, 1874, 2772
BsePI GCGCGC 1 cut(s) 1398
BseRI GAGGAG 7 cut(s) 307, 353, 992, 1112, 1213, 1478, 1950
BseSI GKGCMC 1 cut(s) 1869
BsgI GTGCAG 1 cut(s) 725
Bsh1236I CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
Bsh1285I CGRYCG 2 cut(s) 331, 2909
BshFI GGCC 3 cut(s) 701, 1232, 1286
BshNI GGYRCC 1 cut(s) 1568
BsiEI CGRYCG 2 cut(s) 331, 2909
BsiHKAI GWGCWC 4 cut(s) 72, 600, 828, 1310
BsiHKCI CYCGRG 3 cut(s) 561, 929, 1433
BsiSI CCGG 9 cut(s) 123, 348, 818, 911, 966, 1046, 1302, 2443, 2909
BsiWI CGTACG 1 cut(s) 843
BslFI GGGAC 3 cut(s) 696, 827, 1201
BslI CCNNNNNNNGG 7 cut(s) 917, 956, 2367, 2612, 2778, 2896, 2992
BsmAI GTCTC 5 cut(s) 34, 423, 2013, 2521, 3037
BsmBI CGTCTC 2 cut(s) 34, 2013
BsmFI GGGAC 3 cut(s) 696, 827, 1201
BsmI GAATGC 3 cut(s) 1137, 1900, 1919
BsnI GGCC 3 cut(s) 701, 1232, 1286
BsoBI CYCGRG 3 cut(s) 561, 929, 1433
Bsp119I TTCGAA 4 cut(s) 747, 1530, 2180, 3032
Bsp1286I GDGCHC 5 cut(s) 72, 600, 828, 1310, 1869
Bsp13I TCCGGA 1 cut(s) 965
Bsp1407I TGTACA 1 cut(s) 1710
Bsp1720I GCTNAGC 2 cut(s) 1023, 1223
Bsp19I CCATGG 1 cut(s) 2611
BspANI GGCC 3 cut(s) 701, 1232, 1286
BspCNI CTCAG 7 cut(s) 574, 1023, 1036, 1176, 1453, 2150, 3064
BspEI TCCGGA 1 cut(s) 965
BspFNI CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 6 cut(s) 640, 815, 816, 1561, 1570, 2576
BspMAI CTGCAG 3 cut(s) 492, 1834, 2791
BspMI ACCTGC 1 cut(s) 2389
BspPI GGATC 3 cut(s) 581, 1948, 2945
BspQI GCTCTTC 2 cut(s) 1681, 2656
BspT104I TTCGAA 4 cut(s) 747, 1530, 2180, 3032
BspT107I GGYRCC 1 cut(s) 1568
BsrBI CCGCTC 2 cut(s) 850, 877
BsrDI GCAATG 1 cut(s) 1535
BsrFI RCCGGY 1 cut(s) 2442
BsrGI TGTACA 1 cut(s) 1710
BsrI ACTGG 7 cut(s) 701, 714, 829, 872, 1662, 1874, 2772
BssAI RCCGGY 1 cut(s) 2442
BssECI CCNNGG 7 cut(s) 273, 562, 1233, 2542, 2611, 2666, 3084
BssHII GCGCGC 1 cut(s) 1398
BssNI GRCGYC 2 cut(s) 5, 630
BssT1I CCWWGG 5 cut(s) 273, 1233, 2542, 2611, 2666
Bst2UI CCWGG 3 cut(s) 1443, 1799, 2987
Bst4CI ACNGT 7 cut(s) 337, 454, 1172, 2025, 2329, 2705, 2802
Bst6I CTCTTC 2 cut(s) 1681, 2656
BstACI GRCGYC 2 cut(s) 5, 630
BstAPI GCANNNNNTGC 3 cut(s) 613, 1841, 1906
BstAUI TGTACA 1 cut(s) 1710
BstBI TTCGAA 4 cut(s) 747, 1530, 2180, 3032
BstDSI CCRYGG 2 cut(s) 2611, 3084
BstF5I GGATG 6 cut(s) 1243, 1438, 1485, 1743, 1986, 3061
BstFNI CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
BstH2I RGCGCY 1 cut(s) 2353
BstHHI GCGC 6 cut(s) 77, 170, 542, 1400, 1402, 2352
BstMAI GTCTC 5 cut(s) 34, 423, 2013, 2521, 3037
BstMCI CGRYCG 2 cut(s) 331, 2909
BstNI CCWGG 3 cut(s) 1443, 1799, 2987
BstNSI RCATGY 1 cut(s) 2256
BstSCI CCNGG 6 cut(s) 816, 1301, 1441, 1797, 2908, 2985
BstSFI CTRYAG 6 cut(s) 265, 488, 972, 1830, 2655, 2787
BstSLI GKGCMC 1 cut(s) 1869
BstUI CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
BstV2I GAAGAC 6 cut(s) 524, 756, 894, 2396, 2626, 2706
BstX2I RGATCY 2 cut(s) 1940, 2240
BstYI RGATCY 2 cut(s) 1940, 2240
BsuI GTATCC 2 cut(s) 1113, 1795
BsuRI GGCC 3 cut(s) 701, 1232, 1286
BtgI CCRYGG 2 cut(s) 2611, 3084
BtgZI GCGATG 2 cut(s) 162, 208
BtsCI GGATG 6 cut(s) 1243, 1438, 1485, 1743, 1986, 3061
BtsI GCAGTG 2 cut(s) 537, 2796
BtsIMutI CAGTG 5 cut(s) 537, 1867, 2779, 2796, 2798
BveI ACCTGC 1 cut(s) 2389
CaiI CAGNNNCTG 2 cut(s) 1030, 2992
CciI TCATGA 1 cut(s) 589
CfoI GCGC 6 cut(s) 77, 170, 542, 1400, 1402, 2352
Cfr10I RCCGGY 1 cut(s) 2442
Cfr13I GGNCC 5 cut(s) 382, 639, 699, 814, 852
Cfr42I CCGCGG 1 cut(s) 3087
CpoI CGGWCCG 1 cut(s) 852
CseI GACGC 4 cut(s) 377, 587, 698, 3095
Csp6I GTAC 4 cut(s) 844, 1469, 1711, 1763
CspI CGGWCCG 1 cut(s) 852
CviQI GTAC 4 cut(s) 844, 1469, 1711, 1763
DrdI GACNNNNNNGTC 2 cut(s) 10, 335
DseDI GACNNNNNNGTC 2 cut(s) 10, 335
EaeI YGGCCR 2 cut(s) 1230, 1284
Eam1104I CTCTTC 2 cut(s) 1681, 2656
EarI CTCTTC 2 cut(s) 1681, 2656
EciI GGCGGA 2 cut(s) 190, 676
Ecl136II GAGCTC 3 cut(s) 70, 598, 1308
Eco130I CCWWGG 5 cut(s) 273, 1233, 2542, 2611, 2666
Eco24I GRGCYC 3 cut(s) 72, 600, 1310
Eco47I GGWCC 4 cut(s) 382, 639, 814, 852
Eco53kI GAGCTC 3 cut(s) 70, 598, 1308
Eco57I CTGAAG 3 cut(s) 125, 2903, 3092
Eco88I CYCGRG 3 cut(s) 561, 929, 1433
EcoICRI GAGCTC 3 cut(s) 70, 598, 1308
EcoO109I RGGNCCY 2 cut(s) 382, 814
EcoRII CCWGG 3 cut(s) 1441, 1797, 2985
EcoT14I CCWWGG 5 cut(s) 273, 1233, 2542, 2611, 2666
EcoT22I ATGCAT 1 cut(s) 2258
EcoT38I GRGCYC 3 cut(s) 72, 600, 1310
ErhI CCWWGG 5 cut(s) 273, 1233, 2542, 2611, 2666
Esp3I CGTCTC 2 cut(s) 34, 2013
FalI AAGNNNNNCTT 2 cut(s) 2391, 2423
FaqI GGGAC 3 cut(s) 696, 827, 1201
FauI CCCGC 4 cut(s) 119, 173, 804, 2357
FauNDI CATATG 1 cut(s) 1882
FokI GGATG 6 cut(s) 1250, 1425, 1492, 1730, 1973, 3068
FriOI GRGCYC 3 cut(s) 72, 600, 1310
FspBI CTAG 9 cut(s) 41, 569, 1278, 1356, 1638, 1727, 2534, 2579, 2826
GlaI GCGC 6 cut(s) 76, 169, 541, 1399, 1401, 2351
GsuI CTGGAG 3 cut(s) 409, 812, 959
HaeII RGCGCY 1 cut(s) 2353
HaeIII GGCC 3 cut(s) 701, 1232, 1286
HapII CCGG 9 cut(s) 123, 348, 818, 911, 966, 1046, 1302, 2443, 2909
HgaI GACGC 4 cut(s) 377, 587, 698, 3095
HhaI GCGC 6 cut(s) 77, 170, 542, 1400, 1402, 2352
Hin1I GRCGYC 2 cut(s) 5, 630
Hin6I GCGC 6 cut(s) 75, 168, 540, 1398, 1400, 2350
HinP1I GCGC 6 cut(s) 75, 168, 540, 1398, 1400, 2350
HincII GTYRAC 1 cut(s) 2848
HindII GTYRAC 1 cut(s) 2848
HindIII AAGCTT 3 cut(s) 1365, 1696, 2355
HpaII CCGG 9 cut(s) 123, 348, 818, 911, 966, 1046, 1302, 2443, 2909
HphI GGTGA 7 cut(s) 145, 154, 817, 955, 1021, 1800, 2162
Hpy166II GTNNAC 3 cut(s) 760, 2805, 2848
Hpy8I GTNNAC 3 cut(s) 760, 2805, 2848
HpyCH4III ACNGT 7 cut(s) 337, 454, 1172, 2025, 2329, 2705, 2802
HpyCH4IV ACGT 4 cut(s) 5, 630, 756, 842
HpySE526I ACGT 4 cut(s) 5, 630, 756, 842
Hsp92I GRCGYC 2 cut(s) 5, 630
HspAI GCGC 6 cut(s) 75, 168, 540, 1398, 1400, 2350
KflI GGGWCCC 1 cut(s) 814
Kpn2I TCCGGA 1 cut(s) 965
KspI CCGCGG 1 cut(s) 3087
LguI GCTCTTC 2 cut(s) 1681, 2656
LmnI GCTCC 6 cut(s) 320, 831, 968, 1200, 1305, 2923
MaeI CTAG 9 cut(s) 41, 569, 1278, 1356, 1638, 1727, 2534, 2579, 2826
MaeII ACGT 4 cut(s) 5, 630, 756, 842
MaeIII GTNAC 5 cut(s) 147, 896, 913, 2196, 2329
MbiI CCGCTC 2 cut(s) 850, 877
MfeI CAATTG 1 cut(s) 1839
MflI RGATCY 2 cut(s) 1940, 2240
MhlI GDGCHC 5 cut(s) 72, 600, 828, 1310, 1869
MlsI TGGCCA 2 cut(s) 1232, 1286
MluNI TGGCCA 2 cut(s) 1232, 1286
MlyI GAGTC 5 cut(s) 38, 179, 659, 941, 2967
MmeI TCCRAC 5 cut(s) 633, 657, 2376, 2541, 3095
Mox20I TGGCCA 2 cut(s) 1232, 1286
Mph1103I ATGCAT 1 cut(s) 2258
MroI TCCGGA 1 cut(s) 965
MroXI GAANNNNTTC 3 cut(s) 474, 2403, 2650
MscI TGGCCA 2 cut(s) 1232, 1286
MseI TTAA 8 cut(s) 774, 861, 1155, 1377, 1497, 2061, 2340, 2886
Msp20I TGGCCA 2 cut(s) 1232, 1286
MspA1I CMGCKG 4 cut(s) 145, 793, 2364, 3086
MspI CCGG 9 cut(s) 123, 348, 818, 911, 966, 1046, 1302, 2443, 2909
MspR9I CCNGG 6 cut(s) 818, 1303, 1443, 1799, 2910, 2987
MunI CAATTG 1 cut(s) 1839
Mva1269I GAATGC 3 cut(s) 1137, 1900, 1919
MvaI CCWGG 3 cut(s) 1443, 1799, 2987
MvnI CGCG 6 cut(s) 168, 327, 542, 1400, 3086, 3106
NciI CCSGG 3 cut(s) 818, 1303, 2910
NcoI CCATGG 1 cut(s) 2611
NdeI CATATG 1 cut(s) 1882
NlaIV GGNNCC 6 cut(s) 640, 815, 816, 1561, 1570, 2576
NmeAIII GCCGAG 2 cut(s) 91, 701
NmuCI GTSAC 3 cut(s) 147, 896, 913
NsiI ATGCAT 1 cut(s) 2258
NspI RCATGY 1 cut(s) 2256
NspV TTCGAA 4 cut(s) 747, 1530, 2180, 3032
PaeI GCATGC 1 cut(s) 2256
PaeR7I CTCGAG 2 cut(s) 929, 1433
PagI TCATGA 1 cut(s) 589
PauI GCGCGC 1 cut(s) 1398
PciSI GCTCTTC 2 cut(s) 1681, 2656
PcsI WCGNNNNNNNCGW 6 cut(s) 11, 14, 17, 363, 753, 839
PctI GAATGC 3 cut(s) 1137, 1900, 1919
PdmI GAANNNNTTC 3 cut(s) 474, 2403, 2650
PfeI GAWTC 9 cut(s) 586, 664, 685, 1527, 1754, 2107, 2188, 2591, 2616
Pfl23II CGTACG 1 cut(s) 843
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 2992
PleI GAGTC 5 cut(s) 38, 179, 658, 940, 2966
PpsI GAGTC 5 cut(s) 38, 179, 658, 940, 2966
PpuMI RGGWCCY 2 cut(s) 382, 814
PshBI ATTAAT 1 cut(s) 861
PsiI TTATAA 1 cut(s) 1793
Psp124BI GAGCTC 3 cut(s) 72, 600, 1310
Psp5II RGGWCCY 2 cut(s) 382, 814
Psp6I CCWGG 3 cut(s) 1441, 1797, 2985
PspGI CCWGG 3 cut(s) 1441, 1797, 2985
PspLI CGTACG 1 cut(s) 843
PspN4I GGNNCC 6 cut(s) 640, 815, 816, 1561, 1570, 2576
PspPI GGNCC 5 cut(s) 382, 639, 699, 814, 852
PspPPI RGGWCCY 2 cut(s) 382, 814
PspXI VCTCGAGB 1 cut(s) 1433
PstI CTGCAG 3 cut(s) 492, 1834, 2791
PstNI CAGNNNCTG 2 cut(s) 1030, 2992
PsuI RGATCY 2 cut(s) 1940, 2240
PsyI GACNNNGTC 1 cut(s) 7
PteI GCGCGC 1 cut(s) 1398
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 4 cut(s) 845, 1470, 1712, 1764
RsaNI GTAC 4 cut(s) 844, 1469, 1711, 1763
Rsr2I CGGWCCG 1 cut(s) 852
RsrII CGGWCCG 1 cut(s) 852
SacI GAGCTC 3 cut(s) 72, 600, 1310
SacII CCGCGG 1 cut(s) 3087
SapI GCTCTTC 2 cut(s) 1681, 2656
SaqAI TTAA 8 cut(s) 774, 861, 1155, 1377, 1497, 2061, 2340, 2886
Sau96I GGNCC 5 cut(s) 382, 639, 699, 814, 852
SchI GAGTC 5 cut(s) 38, 179, 659, 941, 2967
ScrFI CCNGG 6 cut(s) 818, 1303, 1443, 1799, 2910, 2987
SduI GDGCHC 5 cut(s) 72, 600, 828, 1310, 1869
SfcI CTRYAG 6 cut(s) 265, 488, 972, 1830, 2655, 2787
Sfr274I CTCGAG 2 cut(s) 929, 1433
Sfr303I CCGCGG 1 cut(s) 3087
SfuI TTCGAA 4 cut(s) 747, 1530, 2180, 3032
SgrBI CCGCGG 1 cut(s) 3087
SinI GGWCC 4 cut(s) 382, 639, 814, 852
SlaI CTCGAG 2 cut(s) 929, 1433
SmlI CTYRAG 5 cut(s) 215, 929, 1346, 1414, 1433
SmoI CTYRAG 5 cut(s) 215, 929, 1346, 1414, 1433
SphI GCATGC 1 cut(s) 2256
SspMI CTAG 9 cut(s) 41, 569, 1278, 1356, 1638, 1727, 2534, 2579, 2826
SstI GAGCTC 3 cut(s) 72, 600, 1310
StyD4I CCNGG 6 cut(s) 816, 1301, 1441, 1797, 2908, 2985
StyI CCWWGG 5 cut(s) 273, 1233, 2542, 2611, 2666
TaaI ACNGT 7 cut(s) 337, 454, 1172, 2025, 2329, 2705, 2802
TaiI ACGT 4 cut(s) 8, 633, 759, 845
TatI WGTACW 2 cut(s) 1710, 1762
TauI GCSGC 4 cut(s) 117, 230, 1520, 2252
TfiI GAWTC 9 cut(s) 586, 664, 685, 1527, 1754, 2107, 2188, 2591, 2616
Tru1I TTAA 8 cut(s) 774, 861, 1155, 1377, 1497, 2061, 2340, 2886
Tru9I TTAA 8 cut(s) 774, 861, 1155, 1377, 1497, 2061, 2340, 2886
TscAI CASTG 5 cut(s) 537, 1874, 2779, 2796, 2805
TseFI GTSAC 3 cut(s) 147, 896, 913
Tsp45I GTSAC 3 cut(s) 147, 896, 913
TspDTI ATGAA 9 cut(s) 449, 467, 1254, 1377, 1912, 2099, 2301, 2626, 2679
TspGWI ACGGA 3 cut(s) 16, 277, 2499
TspRI CASTG 5 cut(s) 537, 1874, 2779, 2796, 2805
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 2992
VpaK11BI GGWCC 4 cut(s) 382, 639, 814, 852
VspI ATTAAT 1 cut(s) 861
XapI RAATTY 3 cut(s) 1014, 1858, 1971
XbaI TCTAGA 1 cut(s) 1726
XceI RCATGY 1 cut(s) 2256
XcmI CCANNNNNNNNNTGG 3 cut(s) 529, 1579, 1991
XhoI CTCGAG 2 cut(s) 929, 1433
XmnI GAANNNNTTC 3 cut(s) 474, 2403, 2650
XspI CTAG 9 cut(s) 41, 569, 1278, 1356, 1638, 1727, 2534, 2579, 2826
ZraI GACGTC 2 cut(s) 6, 631
Zsp2I ATGCAT 1 cut(s) 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.