pycom11g13400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006142
Physical Location & Seq
Forward (+)
12695669 .. 12697544
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1698 bp
ATGGTGAAGAGGGATATACCAATCGATTCGTATACTTGCAATACACTAGTTAAAGGGTTTTGTCAAATTGGGTTGGTAGAGTACGCAGCACGGGTTATGGATAATTTGGTGGATGGAAGAATTACACAGGATGTTGTAGGTTTTAACACCTTGATTGCTGGACATTGCAAAGCTGGACAAGTTAGTCAGGCACTTGAATTGATGGACAGAAGGGGTGGGGAGAGTCTGTCTCCTGATATTATTACTTATAATACTCTGATTCATGGGTTTTGCAGCACGGGTGATTTTGCAAGGGCCAAGAGTCTTATAGATACACTGTTGAGATCTCAGACAAATGAAGAATGCCTTAATGATGAAAGAGACGATGGCCACAATCAGACTGAAGATAAGAATTTGAAACCAAACCTTATAACACACACCACACTTATTAGTTCTTATAGTAAGCGGCAAGGACTTGAAGAAGCTCTGTCTTTGTCTGAGGAAATGGTTATGAATGGCATTTATCTGGATGTACTTGCTTATAGCTCCATTATAAATGGCCTTTGCAAGTATGGGAGGCTATCAGAAGCCAAAGTGCTTTTAAGGGAGATGGAGGAAATGGGTGTGGATCCTAATCATATCTCCTATACTACCCTTGTGGATTTCTTGTTTAAGGCAGGCTCTTCCATGGAAGCTCTTGCCCTTCAAAGCCAAATGGTTGTTTGTGGCCTGGTTTTTGATTTGGTAATCTGTACTGCTTTAATTGTTGGGCTCTTTACGGTTGGGAAATCTGATGATAATCTGTACTTAAAAGAAATGGAGAAGCGCCACATTGTCTCAAATATTATTACTTATTCTTCAATGATAAATGGCTTCGTGAAGGAAGGAAAGCTTGGTGAGGCCATGAGTTTATTAAGGGAGATGGTGCAAAAGAATATCCTGCCAAATGCTTTTGTGTATGCAACACGAATTGACGGGTGCTTTAAGGCAGGTAAACAAGAGCTCGCTCTTGATCTTTACAATGAAATGAACATGGGAGGAGTGGAGGATAACAATTTCGTACTTGATACTTTTTTGAACAACATGAAAAGGCGCAGAAGGATGGAGGAAGCTGAAGGATTAATTATGGATATGACTTCTGGGGGTTTGTTTCTTGATCGCGTTAACTACACATCTCCAATGGATGGGTTTGATGTTGTTGCATACAATGTCTTAATGAATGGTCTATTGAGGCTTGGGAAGTATGAAGCAATATCTGTTTGTACTGGAATGAAAAAGTTGGGTTTGGCTCCTGACTATGCCACATACAATACCATGATTAATGCATTCTGCAGAGAGGGTGACGCTGAAAATGCTTTTGAACTCTGGCATGAGATGAAGTGTCAAGGGTTAATTCCAAACTCAAACACTTGTAACATTCTGATCCAAGGACTTTGCGATGCAAATGAGATTGAAAAAACCATAGATGCCTTGAATGGAATGCTGTCTGTTGGTTCTCTCCCTACCTCATTTATTCATAGAATTCTGCTTGATGCGTCTTCAAAGAGTAGAAGAGTTGATTCAATTTTGCAGGTGCATCATAAGCTTGTATGTATGGGGCTTAACCTCAATCGAGATGTTTATAATAATCTCATCACTGTCTTGTGTAGGCTAGGTATGGCCATGAAAGCCACTTTAGTGTTGAAAGAGATGACTGGAGGAGGATTTTTAGCAGATTGA

Protein Analysis

566

Amino Acids

62.72

Weight (kDa)

5.22

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 44 - 69 3.7e-07 PPR repeat
PPR_2 PF13041 44 - 91 8.1e-15 PPR repeat family
PPR_1 PF12854 76 - 104 1e-08 PPR repeat
PPR_2 PF13041 134 - 183 1.9e-10 PPR repeat family
PPR_3 PF13812 158 - 213 2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 166 - 198 3e-09 PPR repeat
PPR_2 PF13041 170 - 216 6.2e-12 PPR repeat family
PPR PF01535 173 - 202 7e-07 PPR repeat
PPR_3 PF13812 231 - 287 4.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 240 - 287 8.4e-06 PPR repeat family
PPR_1 PF12854 271 - 302 1.3e-08 PPR repeat
PPR_2 PF13041 274 - 322 1.8e-12 PPR repeat family
PPR PF01535 276 - 306 7.8e-09 PPR repeat
PPR_2 PF13041 391 - 438 8.1e-12 PPR repeat family
PPR_long PF17177 405 - 506 1.3e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 410 - 519 2.5e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 414 - 469 1.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 421 - 453 7.1e-12 PPR repeat
PPR_2 PF13041 424 - 473 7.4e-19 PPR repeat family
PPR PF01535 428 - 456 1.2e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 249, 410, 533, 1602
AarI CACCTGC 1 cut(s) 1540
AasI GACNNNNNNGTC 1 cut(s) 183
Acc36I ACCTGC 2 cut(s) 959, 1540
AccB7I CCANNNNNTGG 1 cut(s) 1163
AccI GTMKAC 1 cut(s) 32
AccII CGCG 1 cut(s) 1140
AciI CCGC 1 cut(s) 445
AclWI GGATC 3 cut(s) 602, 615, 1396
AcoI YGGCCR 2 cut(s) 367, 1638
AcsI RAATTY 2 cut(s) 391, 1500
AcuI CTGAAG 2 cut(s) 402, 1113
AfaI GTAC 6 cut(s) 83, 513, 733, 785, 1041, 1243
AfiI CCNNNNNNNGG 1 cut(s) 1163
AhlI ACTAGT 1 cut(s) 46
AjnI CCWGG 1 cut(s) 708
AjuI GAANNNNNNNTTGG 2 cut(s) 855, 887
AleI CACNNNNGTG 1 cut(s) 1655
AluBI AGCT 8 cut(s) 173, 464, 525, 674, 871, 982, 1091, 1564
AluI AGCT 8 cut(s) 173, 464, 525, 674, 871, 982, 1091, 1564
Alw21I GWGCWC 1 cut(s) 984
Alw26I GTCTC 3 cut(s) 234, 354, 820
AlwI GGATC 3 cut(s) 602, 615, 1396
AoxI GGCC 6 cut(s) 294, 367, 538, 706, 879, 1638
ApeKI GCWGC 2 cut(s) 86, 273
ApoI RAATTY 2 cut(s) 391, 1500
AseI ATTAAT 2 cut(s) 1100, 1299
AspLEI GCGC 2 cut(s) 807, 1074
AspS9I GGNCC 1 cut(s) 294
AsuHPI GGTGA 4 cut(s) 16, 293, 887, 1331
BalI TGGCCA 2 cut(s) 369, 1640
BamHI GGATCC 1 cut(s) 607
BanII GRGCYC 2 cut(s) 753, 984
BbsI GAAGAC 1 cut(s) 1509
Bbv12I GWGCWC 1 cut(s) 984
BbvI GCAGC 2 cut(s) 98, 285
BccI CCATC 7 cut(s) 107, 196, 359, 583, 895, 1075, 1157
BciT130I CCWGG 1 cut(s) 710
BcoDI GTCTC 3 cut(s) 234, 354, 820
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 2 cut(s) 47, 1631
BfmI CTRYAG 1 cut(s) 1309
BfoI RGCGCY 1 cut(s) 808
BfuAI ACCTGC 2 cut(s) 959, 1540
BglII AGATCT 1 cut(s) 323
BisI GCNGC 3 cut(s) 87, 274, 446
BlsI GCNGC 3 cut(s) 88, 275, 447
Bme1390I CCNGG 1 cut(s) 710
BmgT120I GGNCC 1 cut(s) 294
BmiI GGNNCC 2 cut(s) 609, 1269
BmrFI CCNGG 1 cut(s) 710
BmsI GCATC 4 cut(s) 1408, 1435, 1501, 1564
BpiI GAAGAC 1 cut(s) 1509
BplI GAGNNNNNCTC 2 cut(s) 214, 246
BpmI CTGGAG 1 cut(s) 1695
Bsa29I ATCGAT 1 cut(s) 24
BsaBI GATNNNNATC 2 cut(s) 612, 777
BsaJI CCNNGG 2 cut(s) 666, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 1163
Bse1I ACTGG 2 cut(s) 1249, 1678
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 2 cut(s) 612, 777
BseBI CCWGG 1 cut(s) 710
BseCI ATCGAT 1 cut(s) 24
BseDI CCNNGG 2 cut(s) 666, 1405
BseGI GGATG 5 cut(s) 118, 136, 514, 1086, 1168
BseJI GATNNNNATC 2 cut(s) 612, 777
BseLI CCNNNNNNNGG 1 cut(s) 1163
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 341, 468
BseNI ACTGG 2 cut(s) 1249, 1678
BseRI GAGGAG 2 cut(s) 1032, 1692
BseXI GCAGC 2 cut(s) 98, 285
Bsh1236I CGCG 1 cut(s) 1140
BshFI GGCC 6 cut(s) 296, 369, 540, 708, 881, 1640
BshVI ATCGAT 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 984
BslI CCNNNNNNNGG 1 cut(s) 1163
BsmAI GTCTC 3 cut(s) 234, 354, 820
BsmBI CGTCTC 1 cut(s) 354
BsmI GAATGC 3 cut(s) 347, 1304, 1464
BsnI GGCC 6 cut(s) 296, 369, 540, 708, 881, 1640
Bsp1286I GDGCHC 2 cut(s) 753, 984
Bsp143I GATC 5 cut(s) 323, 607, 991, 1135, 1401
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 1 cut(s) 445
BspANI GGCC 6 cut(s) 296, 369, 540, 708, 881, 1640
BspCNI CTCAG 2 cut(s) 340, 469
BspDI ATCGAT 1 cut(s) 24
BspFNI CGCG 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 609, 1269
BspMAI CTGCAG 1 cut(s) 1313
BspMI ACCTGC 2 cut(s) 959, 1540
BspPI GGATC 3 cut(s) 602, 615, 1396
BspQI GCTCTTC 1 cut(s) 667
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 2 cut(s) 1249, 1678
BssECI CCNNGG 2 cut(s) 666, 1405
BssMI GATC 5 cut(s) 323, 607, 991, 1135, 1401
BssNAI GTATAC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 666, 1405
Bst1107I GTATAC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 318, 760, 1618
Bst6I CTCTTC 3 cut(s) 2, 667, 1525
BstC8I GCNNGC 2 cut(s) 658, 984
BstDEI CTNAG 2 cut(s) 327, 477
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 5 cut(s) 118, 136, 514, 1086, 1168
BstFNI CGCG 1 cut(s) 1140
BstH2I RGCGCY 1 cut(s) 808
BstHHI GCGC 2 cut(s) 807, 1074
BstKTI GATC 5 cut(s) 326, 610, 994, 1138, 1404
BstMAI GTCTC 3 cut(s) 234, 354, 820
BstMBI GATC 5 cut(s) 323, 607, 991, 1135, 1401
BstMWI GCNNNNNNNGC 3 cut(s) 1331, 1561, 1646
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 1 cut(s) 708
BstSFI CTRYAG 1 cut(s) 1309
BstUI CGCG 1 cut(s) 1140
BstV1I GCAGC 2 cut(s) 98, 285
BstV2I GAAGAC 1 cut(s) 1509
BstX2I RGATCY 2 cut(s) 323, 607
BstYI RGATCY 2 cut(s) 323, 607
BstZ17I GTATAC 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 24
BsuRI GGCC 6 cut(s) 296, 369, 540, 708, 881, 1640
BsuTUI ATCGAT 1 cut(s) 24
BtgI CCRYGG 1 cut(s) 666
BtgZI GCGATG 1 cut(s) 1431
BtsCI GGATG 5 cut(s) 118, 136, 514, 1086, 1168
BtsIMutI CAGTG 2 cut(s) 314, 1614
BveI ACCTGC 2 cut(s) 959, 1540
Cac8I GCNNGC 2 cut(s) 658, 984
CfoI GCGC 2 cut(s) 807, 1074
Cfr13I GGNCC 1 cut(s) 294
ClaI ATCGAT 1 cut(s) 24
CseI GACGC 2 cut(s) 1331, 1503
Csp6I GTAC 6 cut(s) 82, 512, 732, 784, 1040, 1242
CviAII CATG 8 cut(s) 263, 667, 883, 1012, 1063, 1294, 1349, 1642
CviQI GTAC 6 cut(s) 82, 512, 732, 784, 1040, 1242
DdeI CTNAG 2 cut(s) 327, 477
DpnI GATC 5 cut(s) 325, 609, 993, 1137, 1403
DpnII GATC 5 cut(s) 323, 607, 991, 1135, 1401
DrdI GACNNNNNNGTC 1 cut(s) 183
DseDI GACNNNNNNGTC 1 cut(s) 183
EaeI YGGCCR 2 cut(s) 367, 1638
Eam1104I CTCTTC 3 cut(s) 2, 667, 1525
EarI CTCTTC 3 cut(s) 2, 667, 1525
Ecl136II GAGCTC 1 cut(s) 982
Eco130I CCWWGG 2 cut(s) 666, 1405
Eco24I GRGCYC 2 cut(s) 753, 984
Eco53kI GAGCTC 1 cut(s) 982
Eco57I CTGAAG 2 cut(s) 402, 1113
EcoICRI GAGCTC 1 cut(s) 982
EcoRI GAATTC 1 cut(s) 1500
EcoRII CCWGG 1 cut(s) 708
EcoT14I CCWWGG 2 cut(s) 666, 1405
EcoT22I ATGCAT 1 cut(s) 1306
EcoT38I GRGCYC 2 cut(s) 753, 984
ErhI CCWWGG 2 cut(s) 666, 1405
Esp3I CGTCTC 1 cut(s) 354
FaeI CATG 8 cut(s) 266, 670, 886, 1015, 1066, 1297, 1352, 1645
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 855, 887
FatI CATG 8 cut(s) 262, 666, 882, 1011, 1062, 1293, 1348, 1641
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 3 cut(s) 87, 274, 446
FokI GGATG 5 cut(s) 125, 143, 521, 1093, 1175
FriOI GRGCYC 2 cut(s) 753, 984
Fsp4HI GCNGC 3 cut(s) 87, 274, 446
FspBI CTAG 2 cut(s) 47, 1631
GlaI GCGC 2 cut(s) 806, 1073
GluI GCNGC 3 cut(s) 87, 274, 446
GsuI CTGGAG 1 cut(s) 1695
HaeII RGCGCY 1 cut(s) 808
HaeIII GGCC 6 cut(s) 296, 369, 540, 708, 881, 1640
HgaI GACGC 2 cut(s) 1331, 1503
HhaI GCGC 2 cut(s) 807, 1074
Hin1II CATG 8 cut(s) 266, 670, 886, 1015, 1066, 1297, 1352, 1645
Hin6I GCGC 2 cut(s) 805, 1072
HinP1I GCGC 2 cut(s) 805, 1072
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 2 cut(s) 869, 1562
HinfI GANTC 5 cut(s) 26, 223, 259, 301, 1538
HpaI GTTAAC 1 cut(s) 1144
HphI GGTGA 4 cut(s) 16, 293, 887, 1331
Hpy166II GTNNAC 3 cut(s) 33, 974, 1144
Hpy188I TCNGA 7 cut(s) 258, 330, 378, 478, 565, 772, 1401
Hpy188III TCNNGA 7 cut(s) 233, 506, 856, 989, 1133, 1271, 1592
Hpy8I GTNNAC 3 cut(s) 33, 974, 1144
HpyAV CCTTC 6 cut(s) 204, 692, 853, 857, 1071, 1088
HpyCH4III ACNGT 3 cut(s) 318, 760, 1618
HpyF10VI GCNNNNNNNGC 3 cut(s) 1331, 1561, 1646
HpyF3I CTNAG 2 cut(s) 327, 477
Hsp92II CATG 8 cut(s) 266, 670, 886, 1015, 1066, 1297, 1352, 1645
HspAI GCGC 2 cut(s) 805, 1072
KspAI GTTAAC 1 cut(s) 1144
Kzo9I GATC 5 cut(s) 323, 607, 991, 1135, 1401
LguI GCTCTTC 1 cut(s) 667
LmnI GCTCC 2 cut(s) 530, 1273
Lsp1109I GCAGC 2 cut(s) 98, 285
LweI GCATC 4 cut(s) 1408, 1435, 1501, 1564
MaeI CTAG 2 cut(s) 47, 1631
MaeIII GTNAC 2 cut(s) 1319, 1391
MalI GATC 5 cut(s) 325, 609, 993, 1137, 1403
MboI GATC 5 cut(s) 323, 607, 991, 1135, 1401
MboII GAAGA 9 cut(s) 19, 129, 350, 395, 470, 654, 828, 1509, 1542
MflI RGATCY 2 cut(s) 323, 607
MhlI GDGCHC 2 cut(s) 753, 984
MlsI TGGCCA 2 cut(s) 369, 1640
MluNI TGGCCA 2 cut(s) 369, 1640
MlyI GAGTC 2 cut(s) 232, 310
Mox20I TGGCCA 2 cut(s) 369, 1640
Mph1103I ATGCAT 1 cut(s) 1306
MscI TGGCCA 2 cut(s) 369, 1640
MslI CAYNNNNRTG 1 cut(s) 1655
Msp20I TGGCCA 2 cut(s) 369, 1640
MspR9I CCNGG 1 cut(s) 710
Mva1269I GAATGC 3 cut(s) 347, 1304, 1464
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 1 cut(s) 1140
MwoI GCNNNNNNNGC 3 cut(s) 1331, 1561, 1646
NcoI CCATGG 1 cut(s) 666
NdeII GATC 5 cut(s) 323, 607, 991, 1135, 1401
NlaIII CATG 8 cut(s) 266, 670, 886, 1015, 1066, 1297, 1352, 1645
NlaIV GGNNCC 2 cut(s) 609, 1269
NmuCI GTSAC 1 cut(s) 1319
NsiI ATGCAT 1 cut(s) 1306
OliI CACNNNNGTG 1 cut(s) 1655
PaqCI CACCTGC 1 cut(s) 1540
PciSI GCTCTTC 1 cut(s) 667
PctI GAATGC 3 cut(s) 347, 1304, 1464
PfeI GAWTC 3 cut(s) 26, 259, 1538
PflMI CCANNNNNTGG 1 cut(s) 1163
PkrI GCNGC 3 cut(s) 88, 275, 447
PleI GAGTC 2 cut(s) 231, 309
PpsI GAGTC 2 cut(s) 231, 309
PshBI ATTAAT 2 cut(s) 1100, 1299
PsiI TTATAA 4 cut(s) 249, 410, 533, 1602
Psp124BI GAGCTC 1 cut(s) 984
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 609, 1269
PspPI GGNCC 1 cut(s) 294
PstI CTGCAG 1 cut(s) 1313
PsuI RGATCY 2 cut(s) 323, 607
RsaI GTAC 6 cut(s) 83, 513, 733, 785, 1041, 1243
RsaNI GTAC 6 cut(s) 82, 512, 732, 784, 1040, 1242
RseI CAYNNNNRTG 1 cut(s) 1655
SacI GAGCTC 1 cut(s) 984
SapI GCTCTTC 1 cut(s) 667
SatI GCNGC 3 cut(s) 87, 274, 446
Sau3AI GATC 5 cut(s) 323, 607, 991, 1135, 1401
Sau96I GGNCC 1 cut(s) 294
SchI GAGTC 2 cut(s) 232, 310
ScrFI CCNGG 1 cut(s) 710
SduI GDGCHC 2 cut(s) 753, 984
SfaNI GCATC 4 cut(s) 1408, 1435, 1501, 1564
SfcI CTRYAG 1 cut(s) 1309
SmiMI CAYNNNNRTG 1 cut(s) 1655
SpeI ACTAGT 1 cut(s) 46
SsiI CCGC 1 cut(s) 445
SspI AATATT 1 cut(s) 823
SspMI CTAG 2 cut(s) 47, 1631
SstI GAGCTC 1 cut(s) 984
StyD4I CCNGG 1 cut(s) 708
StyI CCWWGG 2 cut(s) 666, 1405
TaaI ACNGT 3 cut(s) 318, 760, 1618
TaqI TCGA 2 cut(s) 24, 1591
TatI WGTACW 4 cut(s) 511, 731, 783, 1241
TauI GCSGC 1 cut(s) 448
TfiI GAWTC 3 cut(s) 26, 259, 1538
TscAI CASTG 2 cut(s) 321, 1621
TseFI GTSAC 1 cut(s) 1319
TseI GCWGC 2 cut(s) 86, 273
Tsp45I GTSAC 1 cut(s) 1319
TspRI CASTG 2 cut(s) 321, 1621
Van91I CCANNNNNTGG 1 cut(s) 1163
VspI ATTAAT 2 cut(s) 1100, 1299
XapI RAATTY 2 cut(s) 391, 1500
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 2 cut(s) 47, 1631
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.