pycom12g03180

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004746
Physical Location & Seq
Reverse (-)
2871902 .. 2872473
572 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGCCTACAACAACCAGAAGAAAGCTCCGGTTGAGCAAAGTCACGGTCGACATAGGATGCAGCTGCAGAAAACCTATTAAGCTCTTCCAAATATTCCACCCTAAACCTAAACCCTCCTCCAAAACCCTAAAACAAAACAAGTACAACCACTTCCCTCATCCTTCTTCTTCTTCTTCTTCAACCCCAAGCAACAATAACAACAACAACAAAGTCTACAAAGACGACTGTGCTCCATTTTTAGTCACAAATTCCGACGCAAAAGCCACGAGACCCGTCAGGGGCTTCGGTAGAATCGGCAACGAGAGCGTGGCTGTGGAGAAAGACTCCGAACGAGATATACTCGAAAGACGATCGATCCGGGAGCTTCTCAACTGTTTCCTGCAGCTCAACTCGCCCTGCAACCACGGCATTATTATCAGGGCCTTCACAGTTGATCGATCTGGAATGCTGTTTTCTCTGTCAGGTCTGGACGTTCGACGTTGCTGCACTTTGCGAGCAACAAGTCACATGACTACTAGCTGA

Protein Analysis

174

Amino Acids

19.45

Weight (kDa)

10.1

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 110 - 143 1.4e-08 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 48, 213
AclWI GGATC 1 cut(s) 349
AcsI RAATTY 1 cut(s) 247
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 278
AgsI TTSAA 1 cut(s) 180
AluBI AGCT 6 cut(s) 25, 63, 82, 364, 385, 519
AluI AGCT 6 cut(s) 25, 63, 82, 364, 385, 519
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 1 cut(s) 349
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 4 cut(s) 60, 63, 382, 483
ApoI RAATTY 1 cut(s) 247
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 134, 166
BauI CACGAG 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 232
BbvI GCAGC 4 cut(s) 50, 72, 394, 470
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 2 cut(s) 64, 380
BisI GCNGC 4 cut(s) 61, 64, 383, 484
BlsI GCNGC 4 cut(s) 62, 65, 384, 485
Bme1390I CCNGG 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 420
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 1 cut(s) 47
BplI GAGNNNNNCTC 4 cut(s) 308, 340, 324, 356
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 2 cut(s) 353, 436
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 403
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 278
BseCI ATCGAT 2 cut(s) 353, 436
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 2 cut(s) 62, 157
BseLI CCNNNNNNNGG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 4 cut(s) 50, 72, 394, 470
BsgI GTGCAG 1 cut(s) 469
Bsh1285I CGRYCG 2 cut(s) 48, 353
BshFI GGCC 1 cut(s) 422
BshVI ATCGAT 2 cut(s) 353, 436
BsiEI CGRYCG 2 cut(s) 48, 353
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 28, 358
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 1 cut(s) 422
Bso31I GGTCTC 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 4 cut(s) 350, 354, 433, 437
BspANI GGCC 1 cut(s) 422
BspDI ATCGAT 2 cut(s) 353, 436
BspMAI CTGCAG 2 cut(s) 68, 384
BspPI GGATC 1 cut(s) 349
BspQI GCTCTTC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 4 cut(s) 350, 354, 433, 437
BssSI CACGAG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 265
Bst4CI ACNGT 4 cut(s) 46, 227, 374, 430
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 495
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 2 cut(s) 62, 157
BstKTI GATC 4 cut(s) 353, 357, 436, 440
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 4 cut(s) 350, 354, 433, 437
BstMCI CGRYCG 2 cut(s) 48, 353
BstMWI GCNNNNNNNGC 3 cut(s) 303, 391, 405
BstSCI CCNGG 1 cut(s) 357
BstSFI CTRYAG 2 cut(s) 64, 380
BstV1I GCAGC 4 cut(s) 50, 72, 394, 470
Bsu15I ATCGAT 2 cut(s) 353, 436
BsuRI GGCC 1 cut(s) 422
BsuTUI ATCGAT 2 cut(s) 353, 436
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 2 cut(s) 62, 157
Cac8I GCNNGC 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 420
ClaI ATCGAT 2 cut(s) 353, 436
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 508
CviQI GTAC 1 cut(s) 142
DpnI GATC 4 cut(s) 352, 356, 435, 439
DpnII GATC 4 cut(s) 350, 354, 433, 437
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 420
FaeI CATG 1 cut(s) 511
FaiI YATR 3 cut(s) 53, 338, 509
FatI CATG 1 cut(s) 507
FblI GTMKAC 2 cut(s) 48, 213
Fnu4HI GCNGC 4 cut(s) 61, 64, 383, 484
FokI GGATG 2 cut(s) 69, 144
Fsp4HI GCNGC 4 cut(s) 61, 64, 383, 484
FspBI CTAG 1 cut(s) 516
GluI GCNGC 4 cut(s) 61, 64, 383, 484
HaeIII GGCC 1 cut(s) 422
HapII CCGG 2 cut(s) 28, 358
HgaI GACGC 1 cut(s) 263
Hin1II CATG 1 cut(s) 511
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 291, 323
HpaII CCGG 2 cut(s) 28, 358
Hpy166II GTNNAC 2 cut(s) 49, 214
Hpy188I TCNGA 2 cut(s) 253, 328
Hpy188III TCNNGA 2 cut(s) 441, 467
Hpy8I GTNNAC 2 cut(s) 49, 214
Hpy99I CGWCG 2 cut(s) 257, 480
HpyAV CCTTC 2 cut(s) 171, 433
HpyCH4III ACNGT 4 cut(s) 46, 227, 374, 430
HpyCH4IV ACGT 2 cut(s) 471, 478
HpyCH4V TGCA 5 cut(s) 60, 66, 382, 399, 486
HpyF10VI GCNNNNNNNGC 3 cut(s) 303, 391, 405
HpySE526I ACGT 2 cut(s) 471, 478
Hsp92II CATG 1 cut(s) 511
Kzo9I GATC 4 cut(s) 350, 354, 433, 437
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 3 cut(s) 30, 235, 361
Lsp1109I GCAGC 4 cut(s) 50, 72, 394, 470
LweI GCATC 1 cut(s) 47
MaeI CTAG 1 cut(s) 516
MaeII ACGT 2 cut(s) 471, 478
MaeIII GTNAC 3 cut(s) 40, 241, 503
MalI GATC 4 cut(s) 352, 356, 435, 439
MboI GATC 4 cut(s) 350, 354, 433, 437
MboII GAAGA 7 cut(s) 30, 76, 156, 159, 162, 165, 168
MhlI GDGCHC 1 cut(s) 232
MluCI AATT 1 cut(s) 247
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 3 cut(s) 124, 127, 165
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 2 cut(s) 28, 358
MspR9I CCNGG 1 cut(s) 359
Mva1269I GAATGC 1 cut(s) 450
MwoI GCNNNNNNNGC 3 cut(s) 303, 391, 405
NciI CCSGG 1 cut(s) 359
NdeII GATC 4 cut(s) 350, 354, 433, 437
NlaIII CATG 1 cut(s) 511
NmuCI GTSAC 3 cut(s) 40, 241, 503
PciSI GCTCTTC 1 cut(s) 89
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 4 cut(s) 62, 65, 384, 485
Ple19I CGATCG 1 cut(s) 353
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 420
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PstI CTGCAG 2 cut(s) 68, 384
PvuI CGATCG 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 4 cut(s) 61, 64, 383, 484
Sau3AI GATC 4 cut(s) 350, 354, 433, 437
Sau96I GGNCC 1 cut(s) 420
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 1 cut(s) 232
SfaNI GCATC 1 cut(s) 47
SfcI CTRYAG 2 cut(s) 64, 380
Sse9I AATT 1 cut(s) 247
SspI AATATT 1 cut(s) 93
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 357
TaaI ACNGT 4 cut(s) 46, 227, 374, 430
TaiI ACGT 2 cut(s) 474, 481
TaqI TCGA 5 cut(s) 48, 342, 353, 436, 475
TasI AATT 1 cut(s) 247
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 3 cut(s) 40, 241, 503
TseI GCWGC 4 cut(s) 60, 63, 382, 483
Tsp45I GTSAC 3 cut(s) 40, 241, 503
XapI RAATTY 1 cut(s) 247
XmiI GTMKAC 2 cut(s) 48, 213
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.