pycom12g15750

Starch binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002509
Physical Location & Seq
Forward (+)
18195755 .. 18197673
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGAGAACCCTGTCGAGTCCGAGTTCGAGGGTGGCGGTAGAAAAGAAGTGGGTGGGCAGAGAAAATGTCTTTCCCCGCGAGCTGGAACCTTGTTTCCTCAGGCCAAAGAGAAGTGGGAATCTTCGGTTCTTGAATTTGGTGTGTTTTCTGAAACAGAGCAAGCCCGTGATCAGACCGATCATTTGCCTCTCGTCAAAAACCCAGACAGAATTGGAGATTGCAGATTCCCAAATTCAGGAAACACATCATACAAAAACTATTCATGTGAAATTCCAGTTGCAGAAGGAGTGCTCGTTTGGGCAGGAGTTCCTCATTGTAGGGGATGATCCTATGTTCGGTTTGTGGGATCCTGCAAGCGCAATACCGATGATCTGGTCAAATGGGAATGTGTGGACTGTTGAGCTGGATATACCGGTTGGGAAATCGATTCAGTTCAAGTTCATACTAAAAGAAAGCAGAGGGAATATCTCGTGGCAGCCTGGCCCTGATCGGATTTTCCAGACCTGGGAAACTAAGAACAGGATCACTGTTTCTGAAGATTGGGCAGATGCTGAGCTTCAGAAAATAATAGAGATCGATCGGGTTTCAAATCAAAGTGATGGTTCAAATGTTAACACTGATATGTTGATTATGGATGAGAACCTGACACAGCCGAATGGCGAACTGGCCTTCAATACAACCAATGAACCTAAAATCACCAATAGTAACACTGATTTGGCAGAGAAACCATCAGTAGAACCAAGGAAGGAACGAATTGGTGCTGATAATGGTTCTCCTTCACAAGAGAATATGACCCCTAAATGGCCAAAGGCATACGTGGAACCATATGAGGAACGAGTTGCTGCAAGAACTGCAGTGCTTAATGAAGAGAGAGTTATTTTTCCAAGCGAGGATTCTGCAGCCATCTCAAGTAAGGAGTTGTTGGTGGCAGACAACATTTTCGGAAATAATGGTAGAGCTGCAACAGTAATGAACCTTTCGAGCACTCACATCGAGGGAAGCCTAGTTAACCTTGAAGAAGATCCTGTTCTGATACCTGGGCTGACTCCTTCACCAGCTGTTCCAAATGAAGAAGCGAACAAAGAGGGAATCGAGAAACAGATGTCTTTTGATGGCTCGGTTGGAGCCTTTGAAGCTAAGGATCGCACTATGCCAGAGGTAACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_20

Protein Analysis

390

Amino Acids

43.51

Weight (kDa)

4.95

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 87 - 174 1.5e-20 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AciI CCGC 2 cut(s) 35, 76
AclWI GGATC 6 cut(s) 320, 341, 354, 530, 1016, 1149
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 3 cut(s) 133, 231, 269
AcuI CTGAAG 2 cut(s) 542, 555
AfiI CCNNNNNNNGG 5 cut(s) 82, 235, 335, 505, 801
AgeI ACCGGT 1 cut(s) 412
AgsI TTSAA 7 cut(s) 133, 436, 588, 606, 673, 1016, 1133
AjnI CCWGG 3 cut(s) 478, 503, 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 877, 909
AloI GAACNNNNNNTCC 2 cut(s) 78, 110
AluBI AGCT 6 cut(s) 82, 403, 556, 959, 1058, 1136
AluI AGCT 6 cut(s) 82, 403, 556, 959, 1058, 1136
Alw21I GWGCWC 2 cut(s) 293, 986
AlwI GGATC 6 cut(s) 320, 341, 354, 530, 1016, 1149
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 4 cut(s) 101, 481, 666, 803
ApeKI GCWGC 4 cut(s) 475, 842, 899, 959
ApoI RAATTY 3 cut(s) 133, 231, 269
ArsI GACNNNNNNTTYG 4 cut(s) 165, 197, 923, 955
AsiGI ACCGGT 1 cut(s) 412
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 688, 1044
AxyI CCTNAGG 1 cut(s) 98
BalI TGGCCA 1 cut(s) 805
BamHI GGATCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 469
Bbv12I GWGCWC 2 cut(s) 293, 986
BbvI GCAGC 4 cut(s) 487, 829, 911, 946
BccI CCATC 4 cut(s) 593, 736, 911, 1106
BcgI CGANNNNNNTGC 4 cut(s) 878, 912, 973, 1007
BciT130I CCWGG 3 cut(s) 480, 505, 1038
BclI TGATCA 1 cut(s) 168
BfaI CTAG 1 cut(s) 1004
BfmI CTRYAG 2 cut(s) 852, 897
BisI GCNGC 4 cut(s) 476, 843, 900, 960
BlpI GCTNAGC 1 cut(s) 552
BlsI GCNGC 4 cut(s) 477, 844, 901, 961
Bme1390I CCNGG 3 cut(s) 480, 505, 1038
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 4 cut(s) 87, 348, 822, 1126
BmrFI CCNGG 3 cut(s) 480, 505, 1038
BmsI GCATC 1 cut(s) 538
Bpu10I CCTNAGC 1 cut(s) 1137
Bpu1102I GCTNAGC 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 892
Bsa29I ATCGAT 2 cut(s) 425, 576
BsaAI YACGTR 1 cut(s) 817
BsaJI CCNNGG 3 cut(s) 504, 740, 1037
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 235, 335, 505, 801
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 2 cut(s) 274, 669
Bse21I CCTNAGG 1 cut(s) 98
BseBI CCWGG 3 cut(s) 480, 505, 1038
BseCI ATCGAT 2 cut(s) 425, 576
BseDI CCNNGG 3 cut(s) 504, 740, 1037
BseGI GGATG 2 cut(s) 328, 640
BseLI CCNNNNNNNGG 5 cut(s) 82, 235, 335, 505, 801
BseMII CTCAG 2 cut(s) 112, 543
BseNI ACTGG 2 cut(s) 274, 669
BseXI GCAGC 4 cut(s) 487, 829, 911, 946
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 580
BshFI GGCC 4 cut(s) 103, 483, 668, 805
BshTI ACCGGT 1 cut(s) 412
BshVI ATCGAT 2 cut(s) 425, 576
BsiEI CGRYCG 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 293, 986
BsiSI CCGG 1 cut(s) 413
BslI CCNNNNNNNGG 5 cut(s) 82, 235, 335, 505, 801
BsnI GGCC 4 cut(s) 103, 483, 668, 805
Bsp1286I GDGCHC 2 cut(s) 293, 986
Bsp1720I GCTNAGC 1 cut(s) 552
BspACI CCGC 2 cut(s) 35, 76
BspANI GGCC 4 cut(s) 103, 483, 668, 805
BspCNI CTCAG 2 cut(s) 111, 544
BspDI ATCGAT 2 cut(s) 425, 576
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 87, 348, 822, 1126
BspMAI CTGCAG 2 cut(s) 856, 901
BspPI GGATC 6 cut(s) 320, 341, 354, 530, 1016, 1149
BsrFI RCCGGY 1 cut(s) 412
BsrI ACTGG 2 cut(s) 274, 669
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 3 cut(s) 504, 740, 1037
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 740
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 3 cut(s) 480, 505, 1038
Bst4CI ACNGT 3 cut(s) 397, 529, 967
Bst6I CTCTTC 1 cut(s) 861
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 817
BstC8I GCNNGC 3 cut(s) 80, 161, 355
BstDEI CTNAG 4 cut(s) 98, 513, 552, 1137
BstF5I GGATG 2 cut(s) 328, 640
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 359
BstMCI CGRYCG 1 cut(s) 580
BstMWI GCNNNNNNNGC 2 cut(s) 851, 1133
BstNI CCWGG 3 cut(s) 480, 505, 1038
BstSCI CCNGG 3 cut(s) 478, 503, 1036
BstSFI CTRYAG 2 cut(s) 852, 897
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 4 cut(s) 487, 829, 911, 946
BstX2I RGATCY 2 cut(s) 346, 1021
BstYI RGATCY 2 cut(s) 346, 1021
Bsu15I ATCGAT 2 cut(s) 425, 576
Bsu36I CCTNAGG 1 cut(s) 98
BsuRI GGCC 4 cut(s) 103, 483, 668, 805
BsuTUI ATCGAT 2 cut(s) 425, 576
BtsCI GGATG 2 cut(s) 328, 640
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 4 cut(s) 525, 615, 708, 861
Cac8I GCNNGC 3 cut(s) 80, 161, 355
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 1 cut(s) 482
ClaI ATCGAT 2 cut(s) 425, 576
CspAI ACCGGT 1 cut(s) 412
CviAII CATG 1 cut(s) 263
DdeI CTNAG 4 cut(s) 98, 513, 552, 1137
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 740
Eco57I CTGAAG 2 cut(s) 542, 555
Eco81I CCTNAGG 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 478, 503, 1036
EcoT14I CCWWGG 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 740
FaeI CATG 1 cut(s) 266
FatI CATG 1 cut(s) 262
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 476, 843, 900, 960
FokI GGATG 2 cut(s) 335, 647
Fsp4HI GCNGC 4 cut(s) 476, 843, 900, 960
FspBI CTAG 1 cut(s) 1004
GlaI GCGC 1 cut(s) 358
GluI GCNGC 4 cut(s) 476, 843, 900, 960
HaeIII GGCC 4 cut(s) 103, 483, 668, 805
HapII CCGG 1 cut(s) 413
HhaI GCGC 1 cut(s) 359
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 2 cut(s) 613, 1009
HindII GTYRAC 2 cut(s) 613, 1009
HinfI GANTC 7 cut(s) 16, 118, 224, 427, 893, 1045, 1089
HpaI GTTAAC 2 cut(s) 613, 1009
HpaII CCGG 1 cut(s) 413
HphI GGTGA 2 cut(s) 688, 1044
Hpy166II GTNNAC 3 cut(s) 393, 613, 1009
Hpy188I TCNGA 8 cut(s) 21, 150, 173, 492, 535, 561, 944, 1032
Hpy188III TCNNGA 4 cut(s) 130, 236, 499, 1093
Hpy8I GTNNAC 3 cut(s) 393, 613, 1009
HpyAV CCTTC 5 cut(s) 277, 679, 739, 786, 1059
HpyCH4III ACNGT 3 cut(s) 397, 529, 967
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 7 cut(s) 221, 280, 353, 845, 854, 899, 962
HpyF10VI GCNNNNNNNGC 2 cut(s) 851, 1133
HpyF3I CTNAG 4 cut(s) 98, 513, 552, 1137
HpySE526I ACGT 1 cut(s) 816
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 168
KspAI GTTAAC 2 cut(s) 613, 1009
LmnI GCTCC 1 cut(s) 1124
Lsp1109I GCAGC 4 cut(s) 487, 829, 911, 946
LweI GCATC 1 cut(s) 538
MaeI CTAG 1 cut(s) 1004
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 2 cut(s) 704, 1159
MboII GAAGA 6 cut(s) 113, 548, 878, 1028, 1031, 1082
MflI RGATCY 2 cut(s) 346, 1021
MhlI GDGCHC 2 cut(s) 293, 986
MlsI TGGCCA 1 cut(s) 805
MluCI AATT 5 cut(s) 133, 209, 231, 269, 753
MluNI TGGCCA 1 cut(s) 805
MlyI GAGTC 2 cut(s) 25, 1039
MmeI TCCRAC 1 cut(s) 1102
Mox20I TGGCCA 1 cut(s) 805
MscI TGGCCA 1 cut(s) 805
MseI TTAA 3 cut(s) 612, 861, 1008
MslI CAYNNNNRTG 1 cut(s) 620
Msp20I TGGCCA 1 cut(s) 805
MspA1I CMGCKG 1 cut(s) 1058
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 3 cut(s) 480, 505, 1038
MvaI CCWGG 3 cut(s) 480, 505, 1038
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 851, 1133
NdeI CATATG 1 cut(s) 826
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 4 cut(s) 87, 348, 822, 1126
PfeI GAWTC 5 cut(s) 118, 224, 427, 893, 1089
PinAI ACCGGT 1 cut(s) 412
PkrI GCNGC 4 cut(s) 477, 844, 901, 961
Ple19I CGATCG 1 cut(s) 580
PleI GAGTC 2 cut(s) 24, 1039
PpsI GAGTC 2 cut(s) 24, 1039
Ppu21I YACGTR 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 478, 503, 1036
PspGI CCWGG 3 cut(s) 478, 503, 1036
PspN4I GGNNCC 4 cut(s) 87, 348, 822, 1126
PspPI GGNCC 1 cut(s) 482
PstI CTGCAG 2 cut(s) 856, 901
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 346, 1021
PvuI CGATCG 1 cut(s) 580
PvuII CAGCTG 1 cut(s) 1058
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 3 cut(s) 612, 861, 1008
SatI GCNGC 4 cut(s) 476, 843, 900, 960
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 2 cut(s) 25, 1039
ScrFI CCNGG 3 cut(s) 480, 505, 1038
SduI GDGCHC 2 cut(s) 293, 986
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 2 cut(s) 852, 897
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
Sse9I AATT 5 cut(s) 133, 209, 231, 269, 753
SsiI CCGC 2 cut(s) 35, 76
SspMI CTAG 1 cut(s) 1004
StyD4I CCNGG 3 cut(s) 478, 503, 1036
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 3 cut(s) 397, 529, 967
TaiI ACGT 1 cut(s) 819
TaqI TCGA 7 cut(s) 14, 26, 425, 576, 980, 993, 1092
TaqII GACCGA 1 cut(s) 190
TasI AATT 5 cut(s) 133, 209, 231, 269, 753
TfiI GAWTC 5 cut(s) 118, 224, 427, 893, 1089
Tru1I TTAA 3 cut(s) 612, 861, 1008
Tru9I TTAA 3 cut(s) 612, 861, 1008
TscAI CASTG 4 cut(s) 532, 622, 715, 861
TseI GCWGC 4 cut(s) 475, 842, 899, 959
TspDTI ATGAA 6 cut(s) 251, 430, 699, 879, 986, 1083
TspRI CASTG 4 cut(s) 532, 622, 715, 861
XapI RAATTY 3 cut(s) 133, 231, 269
XspI CTAG 1 cut(s) 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.