pycom12g17220

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002483
Physical Location & Seq
Reverse (-)
19564502 .. 19574398
9897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4029 bp
ATGGCCTCCCGGTTTTCGCAATCCTCGGTGGTGGTGGTCTCCAGCCAGTTGGACGACTGCGCCTCCAGCCACGATGACGGAGATTTGAGCTCGCAGAGAAGGGAATCAGAAACTGCCAGTAGTAGTTATGGAAATGCCACCGCTACCACCGCCACAAGCTTGGCCTACTTGCCCCAAACCACTGTATTGTGCGAACTTCGGCACGATGCTTTCGAGGCTTGCGTGCCCACCGGACCATCGGATAGTGGTTTAGTATCGAAATGGCGGCCTAAGGACCGAATGAAGACGGGATGTGTAGCTCTGGTATTATGTTTAAACATTAGCGTTGATCCGCCCGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCGATTTGCTATGGCTCCACAAAAAGCTCTTGAAACAATTGGTAAAACCTTGAGTCAACAGTACGAAAGGTGGCAACCAAGGGCTCGCTACAAGTTTCAGCTTGATCCTACAGTAGAAGAAGTGAAGAAACTTTGTAATACATGCCGCAAATGTGCCAAGTCTGAGAGAGTTCTTTTCCATTATAATGGACATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTGTTTAATAAGAGTTATACACAGTATATCCCCTTGCCAATTAGTGACCTCGATTCTTGGTTGAAGACCCCTTCAATATATGTGTTTGATTGTTCTGCTGCTGGGATGATAGTCAATTCTTTCATTGAGCTTCATGATTGGAGTAGCTCTAGCTCATCTGGATCTGCAAAGGATTGTATCCTGCTAGCGGCTTGTGAAGCACACGAGACTCTTCCACAGAGTGCTGAATTTCCTGCTGATGTGTTTACTTCTTGCCTCACAACTCCCATCAAGATGGCATTAAGATGGTTCTGCACACGATCATTACTTCATGAGTCTCTTGATTATTCGCTAATAGATAAAATCCCTGGCCGCCAAAATGACCGGAAAACACTTTTGGGCGAGTTGAATTGGATTTTTACTGCAGTCACTGATACGATTGCATGGAATGTTCTCCCTCACGATCTTTTCCAGAGACTGTTCAGACAAGACCTGCTAGTTGCCAGCCTTTTTCGTAATTTTTTACTTGCTGAGCGAATTATGCGATCTGCAAATTGCTCTCCGATCTCTCACCCACAGTTGCCTCCAACCCATCAGCATCATATGTGGGATGCATGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCAGTATTGGTTGAGGATCCTAATGCGGAGTTCCAGCCAAGCCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCATGGGTCTGAAAACAAGAAACCACCAGAGCAATTGCCTATTGTTCTTCAGGTGTTACTTAGTCAATGCCACCGATTTCGAGCTCTGGTTCTTCTGGGAAGATTCCTTGATATGGGACCCTGGGCTGTAGATCTGGCCTTGTCTGTTGGGATATTTCCGTATGTTCTGAAGCTGTTACAAACAATGACACCTGAACTACGACATATTCTTGTATTTATATGGACAAAAATTCTTGCTCTTGATAAGTCATGTCAGGTTGATCTAGTAAAGGATGGGGGTCATACATATTTCATTAGGTTTCTTGATAGTACGGAAGCATATCCAGAACAGCGTGCAATGGCTGCTTTTGTTTTAGCTGTAATTGTTGATGGGCATAGACGTGGGCAGGAGGCTTGTATTGAAGCAGGTTTGATAAATATTTGCTTGAAGCACCTTCAGGGTCCAACTCTAAATGATACACAGACAGAACCACTATTTCTTCAGTGGCTTTGCCTCTGTCTGGGAAAGCTGTGGGAGGATTTTACAGAGGCCCAAATATTTGGTTTGCAGGCACATGCCCCTGCAATATGTGCACCTCTACTTTCTGAGCCCCAACCAGAGGTTAGAGCTTCTGCTGTTTTCGCACTGGGTACCCTTCTTGATGTGGGTTCAGGCTCATGTAGAGATGGTGTTGGTGGAGAAGAAGAATACGATGATGATGAAAAGATTAGAGCTGAAATTAGTCTTATTAGAAGTCTATTAAGTGTTGCTTCAGATGGCAGCCCTCTGGTCAGGGCTGAAGTTGCTGTTGCACTGGGACGCTTTGCCTTTGGCCACAACAAGCACCTCAAGTCAATAGCTGCTGCATATTGGAAACCACAATCTAATTCTCTTTTGAATTCCTTCCCCTCTTTGGCCCATATTAAAGGTTGTGTTGTTTCAACTCAAATTGGTCCACTGTTGAGAGGGACCAATGATAACTCATTGGGTCGAGATGGGAGAGTCTCCACCAGCAGCCCTCTAGCAAGTTCTGGAATTATGCATGGATCACCATTGTCTGACGATTTATCTCATCATTCTGATTCTGGAATATTAAATGATGGTGTGAGCAATGGTGTTGTCAACCACTCAACACCTAAACCCCTGGACAATGCAATGTATTCGCAGTGTGTATTGGCTATGTGTACCTTAGCCAAGGATCCTTCTCCACGCATTGCAAGCATTGGTAGGCGGGTTTTGGCCATCATAGGGATTGAACAAGTGGTGGCCAAACCCGTAAAGTCCACTGGTAACAGTGTTCGACCTGGTGAATCCATTACAGCAACTCCGACTCCCAGTTTTGGTGGATTAGCTCGGTCTTCTTCATGGTTAGATATGAATGGAGGTCACATGCCATTCCGAACTCCTCCTGTTAGCCCTCCTCGGCCAAATCACTTAACTGGAATACGGAGAGTATGCTCTTTAGACTTCAGGCCTCATCTAATGAGTCCAGATTCTGGATTAGCGGATCCACTTTTAGGTTCTGGGGGAACTTCTGGAGCCTCCGAATGCAGTTTTCTTCCTCAATCAGCCATCTACAATTGGGGTTGCGGGCACTTTTCCAAGCCACTTCTCTTTGTGGCGGATGACAGTAAAGAAGTACTAAGTAGAAGAGAGGAGAGAGAAAAATTTTCCTTGGAGCACATTGCTAAATGCCAGCACTCTTCTGTGAGCAAGCTTAATAATCAAATTGCTAGCTGGGACACCAAGTTTGAGACGGGTACAAAAACAATCTTGTTGAAACCATTTTCTCCTATTGTGGTCGGTGCAGACGAGAATGAGCAAATCAGGGTGTGGAATTACCAAGAGCAGAAGGAGGCTACCCTTCTCAACAGCTTCGATAATCATGATTTTCCTGACAAAGGAATCTCCAAGCTCTGCCTTGTCAATGAGCTTGATGACAGCTTGCTTCTTGCTGCTTCAAGTGATGGAAACATTCGAATTTGGAAAGATTACACTTTGAAGGGTCAACAGAAGCTTGTGACTGCATTTTCTTCAATTCAAGGTCATAAACCTGGGGTGCGAAGTTTGAATGCTGTTGTGGATTGGCAACAACAATCTGGATATCTGTATGCTTCTGGTGAACTATCATCCATTATGCTTTGGGATCTGGATAAAGAGCAGCTTATTAATTCTATTCCTTCTTCATCAGATTGTAGCATCTCAGCACTGTCTGCTTCTCAAGTTCACGGGGGTCACTTTGCAGCTGGTTTTGTAGATGGTTCTGTCAGACTTTATGATGTGCGAACTCCTGAGATGCTTGTCTGTTCAACCCAGCCACACACTCAGAAGGTGGAAAGAGTTGTGGGGATCGGCTTTCAACCCGGGATCGATCCTTCCAAGATTGTAAGTGCATCTCAAGCTGGTGACATTCAGTTCCTTGATATCAGAAATGACAGGGATGCCTACCTCACGATTGAAGCTCACCGGGGTTCACTGACTGCTTTAGCTGTTCATAGGCATGCCCCAATCATTGCCAGTGGCTCGGCCAAACAGCTCATAAAAGTCTTCAGTTTGGAGGGTGAACAATTAGGCACAATAAGATACTACCCTTCCTTTATGGCCCAAAAGATTGGTCCTGTTAGTTCCCTAGCCTTCCATCCCTACGAAGTGCTGCTTGCAGCTGGTGCTGCAGATGCTTGCGCATCCATTTATGCCGACGACAACTCTCAAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1343

Amino Acids

147.27

Weight (kDa)

5.98

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 94 - 245 2.4e-70 Raptor N-terminal CASPase like domain
Beta-prop_WDR36-Utp21_1st PF25171 1024 - 1286 4.6e-11 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 1026 - 1173 3.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 1035 - 1208 2.9e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 1036 - 1204 4.8e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 1037 - 1216 5.1e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 1082 - 1335 1.8e-18 THOC3 beta-propeller domain
WDR55 PF24796 1084 - 1288 4.2e-10 WDR55
WD40_CDC20-Fz PF24807 1127 - 1214 4.3e-06 CDC20/Fizzy WD40 domain
WD40_CDC20-Fz PF24807 1129 - 1333 9e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1140 - 1334 7e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 1227 - 1334 6.1e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 567
Acc16I TGCGCA 1 cut(s) 3991
Acc36I ACCTGC 2 cut(s) 1092, 1724
Acc65I GGTACC 1 cut(s) 1958
AccB1I GGYRCC 1 cut(s) 1958
AccB7I CCANNNNNTGG 1 cut(s) 2804
AcoI YGGCCR 6 cut(s) 961, 2140, 2547, 2574, 2732, 3834
AcsI RAATTY 6 cut(s) 606, 839, 1557, 2206, 2975, 3288
AcuI CTGAAG 8 cut(s) 1361, 1517, 1748, 1793, 2064, 2127, 2761, 3841
AdeI CACNNNGTG 1 cut(s) 833
AfaI GTAC 7 cut(s) 446, 582, 1639, 1960, 2494, 2949, 3070
AhdI GACNNNNNGTC 1 cut(s) 1333
AjiI CACGTC 1 cut(s) 1709
AjnI CCWGG 5 cut(s) 958, 1448, 2451, 2611, 3361
AjuI GAANNNNNNNTTGG 2 cut(s) 971, 1003
Alw21I GWGCWC 4 cut(s) 92, 1414, 1903, 2991
Alw26I GTCTC 6 cut(s) 43, 812, 933, 1060, 2317, 3056
Alw44I GTGCAC 1 cut(s) 1899
AlwNI CAGNNNCTG 4 cut(s) 113, 1022, 1069, 2804
Ama87I CYCGRG 1 cut(s) 3671
ApaLI GTGCAC 1 cut(s) 1899
ApoI RAATTY 6 cut(s) 606, 839, 1557, 2206, 2975, 3288
ArsI GACNNNNNNTTYG 4 cut(s) 3041, 3073, 3907, 3939
AseI ATTAAT 1 cut(s) 3477
Asp700I GAANNNNTTC 1 cut(s) 2210
Asp718I GGTACC 1 cut(s) 1958
AspLEI GCGC 2 cut(s) 62, 3992
AsuC2I CCSGG 4 cut(s) 10, 3672, 3673, 3776
AsuHPI GGTGA 8 cut(s) 614, 1154, 2349, 2627, 3440, 3725, 3764, 3881
AsuII TTCGAA 1 cut(s) 3286
AsuNHI GCTAGC 2 cut(s) 796, 3041
AvaI CYCGRG 1 cut(s) 3671
AvaII GGWCC 7 cut(s) 233, 274, 1445, 1769, 2261, 2277, 3923
AxyI CCTNAGG 1 cut(s) 270
BaeGI GKGCMC 3 cut(s) 228, 1903, 2904
BalI TGGCCA 3 cut(s) 2142, 2549, 2576
BamHI GGATCC 3 cut(s) 1258, 2506, 2815
BanI GGYRCC 1 cut(s) 1958
BanII GRGCYC 4 cut(s) 92, 469, 1414, 1920
BauI CACGAG 1 cut(s) 815
BbsI GAAGAC 4 cut(s) 290, 683, 2658, 3847
Bbv12I GWGCWC 4 cut(s) 92, 1414, 1903, 2991
BcgI CGANNNNNNTGC 2 cut(s) 2451, 2485
BciT130I CCWGG 5 cut(s) 960, 1450, 2453, 2613, 3363
BciVI GTATCC 1 cut(s) 800
BcnI CCSGG 4 cut(s) 10, 3672, 3673, 3776
BcoDI GTCTC 6 cut(s) 43, 812, 933, 1060, 2317, 3056
BfaI CTAG 8 cut(s) 762, 797, 1088, 1592, 2330, 3042, 3938, 4023
BfmI CTRYAG 4 cut(s) 492, 1014, 1455, 3978
BfuAI ACCTGC 2 cut(s) 1092, 1724
BfuI GTATCC 1 cut(s) 800
BglII AGATCT 1 cut(s) 1459
BlpI GCTNAGC 1 cut(s) 1122
BmcAI AGTACT 1 cut(s) 2949
Bme1390I CCNGG 9 cut(s) 10, 960, 1450, 2453, 2613, 3363, 3672, 3673, 3776
Bme18I GGWCC 7 cut(s) 233, 274, 1445, 1769, 2261, 2277, 3923
BmeRI GACNNNNNGTC 1 cut(s) 1333
BmeT110I CYCGRG 1 cut(s) 3671
BmgBI CACGTC 1 cut(s) 1709
BmrFI CCNGG 9 cut(s) 10, 960, 1450, 2453, 2613, 3363, 3672, 3673, 3776
BmrI ACTGGG 3 cut(s) 1964, 2132, 2637
BmsI GCATC 9 cut(s) 196, 1192, 1198, 3516, 3594, 3710, 3739, 3973, 4001
BmtI GCTAGC 2 cut(s) 800, 3045
BmuI ACTGGG 3 cut(s) 1964, 2132, 2637
BpiI GAAGAC 4 cut(s) 290, 683, 2658, 3847
BpmI CTGGAG 3 cut(s) 25, 49, 2865
Bpu10I CCTNAGC 1 cut(s) 2497
Bpu1102I GCTNAGC 1 cut(s) 1122
Bpu14I TTCGAA 1 cut(s) 3286
BpuEI CTTGAG 5 cut(s) 454, 2141, 3513, 3690, 4002
BpuMI CCSGG 4 cut(s) 10, 3672, 3673, 3776
Bsa29I ATCGAT 2 cut(s) 385, 3678
BsaI GGTCTC 1 cut(s) 43
BsaWI WCCGGW 2 cut(s) 230, 975
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 2617, 2623, 2647, 2653
Bse1I ACTGG 9 cut(s) 46, 117, 1244, 1959, 2127, 2599, 2643, 2752, 3825
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 6 cut(s) 1671, 2425, 2469, 2520, 2991, 3819
BseBI CCWGG 5 cut(s) 960, 1450, 2453, 2613, 3363
BseCI ATCGAT 2 cut(s) 385, 3678
BseGI GGATG 9 cut(s) 296, 723, 1207, 1606, 2938, 3437, 3754, 3946, 3992
BseMI GCAATG 6 cut(s) 1671, 2425, 2469, 2520, 2991, 3819
BseMII CTCAG 9 cut(s) 537, 1113, 1212, 1250, 1287, 1905, 3525, 3591, 3647
BseNI ACTGG 9 cut(s) 46, 117, 1244, 1959, 2127, 2599, 2643, 2752, 3825
BseRI GAGGAG 3 cut(s) 2703, 2718, 2978
BseSI GKGCMC 3 cut(s) 228, 1903, 2904
BseYI CCCAGC 3 cut(s) 713, 3045, 3621
BsgI GTGCAG 2 cut(s) 889, 3135
BshNI GGYRCC 1 cut(s) 1958
BshVI ATCGAT 2 cut(s) 385, 3678
BsiHKAI GWGCWC 4 cut(s) 92, 1414, 1903, 2991
BsiHKCI CYCGRG 1 cut(s) 3671
BsiSI CCGG 5 cut(s) 10, 231, 976, 3672, 3775
BslFI GGGAC 5 cut(s) 1223, 1458, 2139, 2290, 3062
BsmAI GTCTC 6 cut(s) 43, 812, 933, 1060, 2317, 3056
BsmBI CGTCTC 1 cut(s) 3056
BsmFI GGGAC 5 cut(s) 1223, 1458, 2139, 2290, 3062
BsmI GAATGC 2 cut(s) 2861, 3385
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 3671
Bsp119I TTCGAA 1 cut(s) 3286
Bsp1286I GDGCHC 8 cut(s) 92, 228, 469, 1414, 1903, 1920, 2904, 2991
Bsp1720I GCTNAGC 1 cut(s) 1122
Bsp19I CCATGG 1 cut(s) 1330
BspCNI CTCAG 9 cut(s) 538, 1114, 1213, 1249, 1288, 1906, 3524, 3592, 3646
BspDI ATCGAT 2 cut(s) 385, 3678
BspHI TCATGA 3 cut(s) 745, 922, 3193
BspMAI CTGCAG 2 cut(s) 1018, 3982
BspMI ACCTGC 2 cut(s) 1092, 1724
BspOI GCTAGC 2 cut(s) 800, 3045
BspT104I TTCGAA 1 cut(s) 3286
BspT107I GGYRCC 1 cut(s) 1958
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 6 cut(s) 1671, 2425, 2469, 2520, 2991, 3819
BsrI ACTGG 9 cut(s) 46, 117, 1244, 1959, 2127, 2599, 2643, 2752, 3825
BssSI CACGAG 1 cut(s) 815
BssT1I CCWWGG 4 cut(s) 461, 1330, 2502, 2982
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 5 cut(s) 960, 1450, 2453, 2613, 3363
Bst6I CTCTTC 3 cut(s) 828, 2953, 3016
BstAPI GCANNNNNTGC 4 cut(s) 1670, 1898, 3521, 3974
BstBI TTCGAA 1 cut(s) 3286
BstDSI CCRYGG 1 cut(s) 1330
BstENI CCTNNNNNAGG 1 cut(s) 3207
BstF5I GGATG 9 cut(s) 296, 723, 1207, 1606, 2938, 3437, 3754, 3946, 3992
BstHHI GCGC 2 cut(s) 62, 3992
BstMAI GTCTC 6 cut(s) 43, 812, 933, 1060, 2317, 3056
BstNI CCWGG 5 cut(s) 960, 1450, 2453, 2613, 3363
BstNSI RCATGY 4 cut(s) 528, 1886, 2701, 3812
BstSCI CCNGG 9 cut(s) 8, 958, 1448, 2451, 2611, 3361, 3670, 3671, 3774
BstSFI CTRYAG 4 cut(s) 492, 1014, 1455, 3978
BstSLI GKGCMC 3 cut(s) 228, 1903, 2904
BstV2I GAAGAC 4 cut(s) 290, 683, 2658, 3847
BstX2I RGATCY 6 cut(s) 773, 1258, 1459, 2506, 2815, 3454
BstXI CCANNNNNNTGG 6 cut(s) 49, 160, 569, 886, 1868, 3920
BstYI RGATCY 6 cut(s) 773, 1258, 1459, 2506, 2815, 3454
Bsu15I ATCGAT 2 cut(s) 385, 3678
Bsu36I CCTNAGG 1 cut(s) 270
BsuI GTATCC 1 cut(s) 800
BsuTUI ATCGAT 2 cut(s) 385, 3678
BtgI CCRYGG 1 cut(s) 1330
BtrI CACGTC 1 cut(s) 1709
BtsCI GGATG 9 cut(s) 296, 723, 1207, 1606, 2938, 3437, 3754, 3946, 3992
BtsI GCAGTG 1 cut(s) 2480
BveI ACCTGC 2 cut(s) 1092, 1724
CaiI CAGNNNCTG 4 cut(s) 113, 1022, 1069, 2804
CciI TCATGA 3 cut(s) 745, 922, 3193
CfoI GCGC 2 cut(s) 62, 3992
Cfr9I CCCGGG 1 cut(s) 3671
ClaI ATCGAT 2 cut(s) 385, 3678
CseI GACGC 1 cut(s) 2136
CsiI ACCWGGT 1 cut(s) 2611
Csp6I GTAC 7 cut(s) 445, 581, 1638, 1959, 2493, 2948, 3069
CviQI GTAC 7 cut(s) 445, 581, 1638, 1959, 2493, 2948, 3069
DraI TTTAAA 1 cut(s) 315
DraIII CACNNNGTG 1 cut(s) 833
DriI GACNNNNNGTC 1 cut(s) 1333
EaeI YGGCCR 6 cut(s) 961, 2140, 2547, 2574, 2732, 3834
Eam1104I CTCTTC 3 cut(s) 828, 2953, 3016
Eam1105I GACNNNNNGTC 1 cut(s) 1333
EarI CTCTTC 3 cut(s) 828, 2953, 3016
EciI GGCGGA 2 cut(s) 321, 2945
Ecl136II GAGCTC 2 cut(s) 90, 1412
Eco130I CCWWGG 4 cut(s) 461, 1330, 2502, 2982
Eco147I AGGCCT 1 cut(s) 2782
Eco24I GRGCYC 4 cut(s) 92, 469, 1414, 1920
Eco31I GGTCTC 1 cut(s) 43
Eco32I GATATC 3 cut(s) 351, 3413, 3733
Eco47I GGWCC 7 cut(s) 233, 274, 1445, 1769, 2261, 2277, 3923
Eco53kI GAGCTC 2 cut(s) 90, 1412
Eco57I CTGAAG 8 cut(s) 1361, 1517, 1748, 1793, 2064, 2127, 2761, 3841
Eco81I CCTNAGG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 3671
EcoICRI GAGCTC 2 cut(s) 90, 1412
EcoNI CCTNNNNNAGG 1 cut(s) 3207
EcoO109I RGGNCCY 1 cut(s) 1445
EcoRI GAATTC 1 cut(s) 2206
EcoRII CCWGG 5 cut(s) 958, 1448, 2451, 2611, 3361
EcoRV GATATC 3 cut(s) 351, 3413, 3733
EcoT14I CCWWGG 4 cut(s) 461, 1330, 2502, 2982
EcoT22I ATGCAT 2 cut(s) 1207, 2352
EcoT38I GRGCYC 4 cut(s) 92, 469, 1414, 1920
ErhI CCWWGG 4 cut(s) 461, 1330, 2502, 2982
Esp3I CGTCTC 1 cut(s) 3056
FalI AAGNNNNNCTT 6 cut(s) 2062, 2094, 3456, 3488, 3948, 3980
FaqI GGGAC 5 cut(s) 1223, 1458, 2139, 2290, 3062
FauI CCCGC 2 cut(s) 2532, 2891
FauNDI CATATG 1 cut(s) 1194
FokI GGATG 9 cut(s) 303, 730, 1214, 1613, 2945, 3424, 3761, 3933, 3979
FriOI GRGCYC 4 cut(s) 92, 469, 1414, 1920
FspBI CTAG 8 cut(s) 762, 797, 1088, 1592, 2330, 3042, 3938, 4023
FspI TGCGCA 1 cut(s) 3991
GlaI GCGC 2 cut(s) 61, 3991
GsaI CCCAGC 3 cut(s) 717, 3049, 3625
GsuI CTGGAG 3 cut(s) 25, 49, 2865
HapII CCGG 5 cut(s) 10, 231, 976, 3672, 3775
HgaI GACGC 1 cut(s) 2136
HhaI GCGC 2 cut(s) 62, 3992
Hin6I GCGC 2 cut(s) 60, 3990
HinP1I GCGC 2 cut(s) 60, 3990
HincII GTYRAC 4 cut(s) 440, 1305, 2431, 3317
HindII GTYRAC 4 cut(s) 440, 1305, 2431, 3317
HindIII AAGCTT 3 cut(s) 157, 3023, 3323
HpaII CCGG 5 cut(s) 10, 231, 976, 3672, 3775
HphI GGTGA 8 cut(s) 614, 1154, 2349, 2627, 3440, 3725, 3764, 3881
Hpy99I CGWCG 1 cut(s) 4010
HpyCH4IV ACGT 1 cut(s) 1708
HpySE526I ACGT 1 cut(s) 1708
HspAI GCGC 2 cut(s) 60, 3990
KflI GGGWCCC 1 cut(s) 1445
KpnI GGTACC 1 cut(s) 1962
LmnI GCTCC 3 cut(s) 403, 2846, 2986
LweI GCATC 9 cut(s) 196, 1192, 1198, 3516, 3594, 3710, 3739, 3973, 4001
MabI ACCWGGT 1 cut(s) 2611
MaeI CTAG 8 cut(s) 762, 797, 1088, 1592, 2330, 3042, 3938, 4023
MaeII ACGT 1 cut(s) 1708
MaeIII GTNAC 9 cut(s) 656, 1018, 1383, 1503, 2597, 2693, 3328, 3542, 3713
MfeI CAATTG 3 cut(s) 420, 1361, 2887
MflI RGATCY 6 cut(s) 773, 1258, 1459, 2506, 2815, 3454
MhlI GDGCHC 8 cut(s) 92, 228, 469, 1414, 1903, 1920, 2904, 2991
MlsI TGGCCA 3 cut(s) 2142, 2549, 2576
MluNI TGGCCA 3 cut(s) 2142, 2549, 2576
MlyI GAGTC 6 cut(s) 445, 814, 935, 2319, 2632, 2803
MmeI TCCRAC 4 cut(s) 30, 1202, 1796, 2660
Mox20I TGGCCA 3 cut(s) 2142, 2549, 2576
Mph1103I ATGCAT 2 cut(s) 1207, 2352
MroXI GAANNNNTTC 1 cut(s) 2210
MscI TGGCCA 3 cut(s) 2142, 2549, 2576
MslI CAYNNNNRTG 5 cut(s) 567, 884, 895, 1707, 3807
Msp20I TGGCCA 3 cut(s) 2142, 2549, 2576
MspA1I CMGCKG 2 cut(s) 3554, 3971
MspI CCGG 5 cut(s) 10, 231, 976, 3672, 3775
MspR9I CCNGG 9 cut(s) 10, 960, 1450, 2453, 2613, 3363, 3672, 3673, 3776
MssI GTTTAAAC 1 cut(s) 315
MunI CAATTG 3 cut(s) 420, 1361, 2887
Mva1269I GAATGC 2 cut(s) 2861, 3385
MvaI CCWGG 5 cut(s) 960, 1450, 2453, 2613, 3363
NciI CCSGG 4 cut(s) 10, 3672, 3673, 3776
NcoI CCATGG 1 cut(s) 1330
NdeI CATATG 1 cut(s) 1194
NheI GCTAGC 2 cut(s) 796, 3041
NmeAIII GCCGAG 2 cut(s) 2710, 3812
NmuCI GTSAC 6 cut(s) 656, 1018, 2693, 3328, 3542, 3713
NsbI TGCGCA 1 cut(s) 3991
NsiI ATGCAT 2 cut(s) 1207, 2352
NspI RCATGY 4 cut(s) 528, 1886, 2701, 3812
NspV TTCGAA 1 cut(s) 3286
PaeI GCATGC 1 cut(s) 3812
PagI TCATGA 3 cut(s) 745, 922, 3193
PasI CCCWGGG 1 cut(s) 1449
PceI AGGCCT 1 cut(s) 2782
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 219, 3117
PctI GAATGC 2 cut(s) 2861, 3385
PdmI GAANNNNTTC 1 cut(s) 2210
PfeI GAWTC 7 cut(s) 104, 665, 1431, 2390, 2618, 2801, 3213
PflMI CCANNNNNTGG 1 cut(s) 2804
PleI GAGTC 6 cut(s) 444, 814, 934, 2318, 2632, 2802
PmeI GTTTAAAC 1 cut(s) 315
PpsI GAGTC 6 cut(s) 444, 814, 934, 2318, 2632, 2802
PpuMI RGGWCCY 1 cut(s) 1445
PshBI ATTAAT 1 cut(s) 3477
PsiI TTATAA 1 cut(s) 567
Psp124BI GAGCTC 2 cut(s) 92, 1414
Psp5II RGGWCCY 1 cut(s) 1445
Psp6I CCWGG 5 cut(s) 958, 1448, 2451, 2611, 3361
PspFI CCCAGC 3 cut(s) 713, 3045, 3621
PspGI CCWGG 5 cut(s) 958, 1448, 2451, 2611, 3361
PspPPI RGGWCCY 1 cut(s) 1445
PstI CTGCAG 2 cut(s) 1018, 3982
PstNI CAGNNNCTG 4 cut(s) 113, 1022, 1069, 2804
PsuI RGATCY 6 cut(s) 773, 1258, 1459, 2506, 2815, 3454
PvuII CAGCTG 2 cut(s) 3554, 3971
RsaI GTAC 7 cut(s) 446, 582, 1639, 1960, 2494, 2949, 3070
RsaNI GTAC 7 cut(s) 445, 581, 1638, 1959, 2493, 2948, 3069
RseI CAYNNNNRTG 5 cut(s) 567, 884, 895, 1707, 3807
SacI GAGCTC 2 cut(s) 92, 1414
ScaI AGTACT 1 cut(s) 2949
SchI GAGTC 6 cut(s) 445, 814, 935, 2319, 2632, 2803
ScrFI CCNGG 9 cut(s) 10, 960, 1450, 2453, 2613, 3363, 3672, 3673, 3776
SduI GDGCHC 8 cut(s) 92, 228, 469, 1414, 1903, 1920, 2904, 2991
SexAI ACCWGGT 1 cut(s) 2611
SfaNI GCATC 9 cut(s) 196, 1192, 1198, 3516, 3594, 3710, 3739, 3973, 4001
SfcI CTRYAG 4 cut(s) 492, 1014, 1455, 3978
SfuI TTCGAA 1 cut(s) 3286
SinI GGWCC 7 cut(s) 233, 274, 1445, 1769, 2261, 2277, 3923
SmaI CCCGGG 1 cut(s) 3673
SmiMI CAYNNNNRTG 5 cut(s) 567, 884, 895, 1707, 3807
SmlI CTYRAG 5 cut(s) 433, 2156, 3528, 3705, 4017
SmoI CTYRAG 5 cut(s) 433, 2156, 3528, 3705, 4017
SphI GCATGC 1 cut(s) 3812
SseBI AGGCCT 1 cut(s) 2782
SspI AATATT 3 cut(s) 1747, 1866, 2400
SspMI CTAG 8 cut(s) 762, 797, 1088, 1592, 2330, 3042, 3938, 4023
SstI GAGCTC 2 cut(s) 92, 1414
StuI AGGCCT 1 cut(s) 2782
StyD4I CCNGG 9 cut(s) 8, 958, 1448, 2451, 2611, 3361, 3670, 3671, 3774
StyI CCWWGG 4 cut(s) 461, 1330, 2502, 2982
TaiI ACGT 1 cut(s) 1711
TaqII GACCGA 2 cut(s) 291, 2652
TatI WGTACW 1 cut(s) 2947
TauI GCSGC 4 cut(s) 268, 531, 803, 966
TfiI GAWTC 7 cut(s) 104, 665, 1431, 2390, 2618, 2801, 3213
TseFI GTSAC 6 cut(s) 656, 1018, 2693, 3328, 3542, 3713
Tsp45I GTSAC 6 cut(s) 656, 1018, 2693, 3328, 3542, 3713
TspGWI ACGGA 4 cut(s) 93, 1476, 1655, 2770
TspMI CCCGGG 1 cut(s) 3671
Van91I CCANNNNNTGG 1 cut(s) 2804
VneI GTGCAC 1 cut(s) 1899
VpaK11BI GGWCC 7 cut(s) 233, 274, 1445, 1769, 2261, 2277, 3923
VspI ATTAAT 1 cut(s) 3477
XagI CCTNNNNNAGG 1 cut(s) 3207
XapI RAATTY 6 cut(s) 606, 839, 1557, 2206, 2975, 3288
XceI RCATGY 4 cut(s) 528, 1886, 2701, 3812
XmaI CCCGGG 1 cut(s) 3671
XmnI GAANNNNTTC 1 cut(s) 2210
XspI CTAG 8 cut(s) 762, 797, 1088, 1592, 2330, 3042, 3938, 4023
ZrmI AGTACT 1 cut(s) 2949
Zsp2I ATGCAT 2 cut(s) 1207, 2352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.