pycom12g20950

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002437
Physical Location & Seq
Forward (+)
22265839 .. 22267360
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGCTACCAAAATCTGCATTATCCAAATTCTCACATTGATTTCCATCTTCTTGCCTCTTTCCAAATCCATCCACTTCAACCACCCTGCCGTTTTCAACTTTGGTGATTCGAATTCCGACACTGGCAACCTTGTTTCCGCAGGCATTGAGACCATTATTTCTCCTTATGGACAATCTTATTTCAAAGCCCCATATGGGAGATACTCCGATGGCCGCCTGATCGTCGATTTTCTTATGGATGCAATGGAGCTTCCGTTTCTCAATGCCTACTTGGATTCAGTTGGCCTGCCGAGTTTCAAAAATGGTTGCAACTTTGCCGCGGCGGGGTCAACTATACTTCCTGCAACTGCAACATCGATCAGCCCATTTTCTTTCGGGGTCCAGGTCTCCCAGTTTTTTAGATTCAAAGCCAAGGTTCTTGAATTGCTAGCTGAAGGTAAGAGCTTTCCCAAGTATCTGCCTACGGAAGATTTTTTTGCGAAAGGGCTCTACATGTTCGATATAGGCCAGAATGATCTCGCTGGTGCATTTTATTCGAAATCATTGGATGAGATTCTCGCGTCTATTCCAACGATTTTGACAGAATTCGAAACTGGAGTAAAGAGATTATATGACCAAGGAGGTAGGAACTTTTGGATACACAACACAGGTCCTCTCGGATGCTTGACGCAGAATGTTGCCAAGTTTGGAACTGATCCCTCGAAGCTTGACGAACAAGGATGCGTGAGCGACCACAACCAAGCTGCTAAACTCTTTAACCTGCAGCTTCATGCTCTCACCAAAAAGTTGCAAGGCCAGTACTCAAATGCAAGTGTCATATATGTTGATATCTTTTCGATCAAATTTGAGCTCATTTCAAATTATTCGAAAAATGGATTTGAGCAACCTATTATGGCTTGCTGCGGGTACGGAGGTCCCCCGTTGAACTACGACAGTCACATTACCTGTGGACAAACAAAGACGTTGAACGGAACAAATGTCACGGCCAAAGGATGCACAGATAGTTCTGATTACATTAGTTGGGATGGAATTCATTACACCGAAGCTGCGAATCAGTATGTCTCGTCCAAGATACTCACCGGGAAGTACTCTGATCCGCCCTTCTCGGATAAAATGCCTTTCCTTCTGAAGCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.79

Weight (kDa)

6.17

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 357 5.7e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 768
AccII CGCG 2 cut(s) 320, 560
AciI CCGC 7 cut(s) 138, 214, 318, 320, 323, 903, 1097
AclWI GGATC 2 cut(s) 689, 1088
AcoI YGGCCR 2 cut(s) 211, 984
AcsI RAATTY 5 cut(s) 27, 112, 584, 842, 1029
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 3 cut(s) 800, 908, 1088
AfiI CCNNNNNNNGG 2 cut(s) 195, 324
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 9 cut(s) 79, 97, 184, 298, 406, 422, 858, 925, 967
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 9 cut(s) 250, 431, 444, 706, 743, 766, 850, 1046, 1132
AluI AGCT 9 cut(s) 250, 431, 444, 706, 743, 766, 850, 1046, 1132
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 3 cut(s) 143, 391, 1066
AlwI GGATC 2 cut(s) 689, 1088
AoxI GGCC 5 cut(s) 211, 283, 505, 793, 984
ApeKI GCWGC 4 cut(s) 743, 763, 900, 1046
ApoI RAATTY 5 cut(s) 27, 112, 584, 842, 1029
AspS9I GGNCC 3 cut(s) 379, 650, 914
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 3 cut(s) 116, 769, 1069
AsuII TTCGAA 4 cut(s) 110, 536, 588, 866
AsuNHI GCTAGC 1 cut(s) 427
AvaII GGWCC 3 cut(s) 379, 650, 914
BaeI ACNNNNGTAYC 2 cut(s) 898, 931
BanII GRGCYC 2 cut(s) 489, 852
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 730, 775, 887, 1033
BccI CCATC 4 cut(s) 53, 77, 203, 1019
BceAI ACGGC 2 cut(s) 74, 999
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BciT130I CCWGG 1 cut(s) 383
BciVI GTATCC 1 cut(s) 630
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 3 cut(s) 143, 391, 1066
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 768
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 7 cut(s) 214, 318, 321, 744, 764, 901, 1047
BlsI GCNGC 7 cut(s) 215, 319, 322, 745, 765, 902, 1048
BmcAI AGTACT 2 cut(s) 800, 1088
Bme1390I CCNGG 2 cut(s) 383, 1081
Bme18I GGWCC 3 cut(s) 379, 650, 914
BmgT120I GGNCC 3 cut(s) 379, 650, 914
BmiI GGNNCC 2 cut(s) 380, 916
BmrFI CCNGG 2 cut(s) 383, 1081
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 4 cut(s) 229, 650, 710, 983
BmtI GCTAGC 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 385
BpmI CTGGAG 1 cut(s) 615
Bpu14I TTCGAA 4 cut(s) 110, 536, 588, 866
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 1 cut(s) 1081
Bsa29I ATCGAT 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 2 cut(s) 143, 391
BsaJI CCNNGG 3 cut(s) 318, 411, 616
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 324
Bse1I ACTGG 4 cut(s) 127, 391, 598, 796
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 318, 411, 616
BseGI GGATG 7 cut(s) 69, 244, 553, 665, 725, 998, 1030
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 2 cut(s) 195, 324
BseMI GCAATG 1 cut(s) 249
BseNI ACTGG 4 cut(s) 127, 391, 598, 796
BseXI GCAGC 4 cut(s) 730, 775, 887, 1033
Bsh1236I CGCG 2 cut(s) 320, 560
BshFI GGCC 5 cut(s) 213, 285, 507, 795, 986
BshVI ATCGAT 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 1 cut(s) 1080
BslFI GGGAC 1 cut(s) 900
BslI CCNNNNNNNGG 2 cut(s) 195, 324
BsmAI GTCTC 3 cut(s) 143, 391, 1066
BsmFI GGGAC 1 cut(s) 900
BsnI GGCC 5 cut(s) 213, 285, 507, 795, 986
Bso31I GGTCTC 2 cut(s) 143, 391
Bsp119I TTCGAA 4 cut(s) 110, 536, 588, 866
Bsp1286I GDGCHC 2 cut(s) 489, 852
Bsp143I GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
BspACI CCGC 7 cut(s) 138, 214, 318, 320, 323, 903, 1097
BspANI GGCC 5 cut(s) 213, 285, 507, 795, 986
BspDI ATCGAT 1 cut(s) 356
BspFNI CGCG 2 cut(s) 320, 560
BspLI GGNNCC 2 cut(s) 380, 916
BspMAI CTGCAG 1 cut(s) 765
BspMI ACCTGC 1 cut(s) 768
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 2 cut(s) 689, 1088
BspT104I TTCGAA 4 cut(s) 110, 536, 588, 866
BspTNI GGTCTC 2 cut(s) 143, 391
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 4 cut(s) 127, 391, 598, 796
BssECI CCNNGG 3 cut(s) 318, 411, 616
BssMI GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
BssT1I CCWWGG 2 cut(s) 411, 616
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 935
BstBI TTCGAA 4 cut(s) 110, 536, 588, 866
BstC8I GCNNGC 4 cut(s) 142, 287, 429, 898
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 7 cut(s) 69, 244, 553, 665, 725, 998, 1030
BstFNI CGCG 2 cut(s) 320, 560
BstKTI GATC 6 cut(s) 222, 360, 517, 697, 840, 1096
BstMAI GTCTC 3 cut(s) 143, 391, 1066
BstMBI GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
BstNI CCWGG 1 cut(s) 383
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 381, 1079
BstSFI CTRYAG 1 cut(s) 761
BstUI CGCG 2 cut(s) 320, 560
BstV1I GCAGC 4 cut(s) 730, 775, 887, 1033
Bsu15I ATCGAT 1 cut(s) 356
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 5 cut(s) 213, 285, 507, 795, 986
BsuTUI ATCGAT 1 cut(s) 356
BtgI CCRYGG 1 cut(s) 318
BtsCI GGATG 7 cut(s) 69, 244, 553, 665, 725, 998, 1030
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 768
Cac8I GCNNGC 4 cut(s) 142, 287, 429, 898
Cfr13I GGNCC 3 cut(s) 379, 650, 914
Cfr42I CCGCGG 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 356
CseI GACGC 2 cut(s) 549, 676
Csp6I GTAC 3 cut(s) 799, 907, 1087
CviAII CATG 2 cut(s) 493, 770
CviQI GTAC 3 cut(s) 799, 907, 1087
DpnI GATC 6 cut(s) 221, 359, 516, 696, 839, 1095
DpnII GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
EaeI YGGCCR 2 cut(s) 211, 984
EciI GGCGGA 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 2 cut(s) 411, 616
Eco24I GRGCYC 2 cut(s) 489, 852
Eco31I GGTCTC 2 cut(s) 143, 391
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 3 cut(s) 379, 650, 914
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 2 cut(s) 650, 914
EcoRI GAATTC 3 cut(s) 112, 584, 1029
EcoRII CCWGG 1 cut(s) 381
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 411, 616
EcoT38I GRGCYC 2 cut(s) 489, 852
ErhI CCWWGG 2 cut(s) 411, 616
FaeI CATG 2 cut(s) 496, 773
FaqI GGGAC 1 cut(s) 900
FatI CATG 2 cut(s) 492, 769
FauI CCCGC 2 cut(s) 316, 896
FauNDI CATATG 1 cut(s) 193
Fnu4HI GCNGC 7 cut(s) 214, 318, 321, 744, 764, 901, 1047
FokI GGATG 7 cut(s) 56, 251, 560, 672, 732, 1005, 1037
FriOI GRGCYC 2 cut(s) 489, 852
Fsp4HI GCNGC 7 cut(s) 214, 318, 321, 744, 764, 901, 1047
FspBI CTAG 1 cut(s) 428
GluI GCNGC 7 cut(s) 214, 318, 321, 744, 764, 901, 1047
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 5 cut(s) 213, 285, 507, 795, 986
HapII CCGG 1 cut(s) 1080
HgaI GACGC 2 cut(s) 549, 676
Hin1II CATG 2 cut(s) 496, 773
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 5 cut(s) 107, 275, 402, 553, 1051
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 3 cut(s) 116, 769, 1069
Hpy166II GTNNAC 2 cut(s) 330, 950
Hpy188I TCNGA 8 cut(s) 118, 208, 659, 1009, 1093, 1108, 1128, 1142
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 2 cut(s) 330, 950
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 3 cut(s) 428, 1111, 1133
HpyCH4III ACNGT 1 cut(s) 935
HpyCH4IV ACGT 1 cut(s) 962
HpySE526I ACGT 1 cut(s) 962
Hsp92II CATG 2 cut(s) 496, 773
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 4 cut(s) 730, 775, 887, 1033
LweI GCATC 4 cut(s) 229, 650, 710, 983
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 2 cut(s) 935, 979
MalI GATC 6 cut(s) 221, 359, 516, 696, 839, 1095
MboI GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
MboII GAAGA 2 cut(s) 40, 479
MhlI GDGCHC 2 cut(s) 489, 852
MluCI AATT 7 cut(s) 27, 112, 422, 584, 842, 859, 1029
MmeI TCCRAC 2 cut(s) 141, 593
MnlI CCTC 5 cut(s) 66, 614, 663, 709, 905
MseI TTAA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 2 cut(s) 383, 1081
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 2 cut(s) 320, 560
NciI CCSGG 1 cut(s) 1081
NdeI CATATG 1 cut(s) 193
NdeII GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 2 cut(s) 496, 773
NlaIV GGNNCC 2 cut(s) 380, 916
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 2 cut(s) 935, 979
NspI RCATGY 1 cut(s) 496
NspV TTCGAA 4 cut(s) 110, 536, 588, 866
PciI ACATGT 1 cut(s) 492
PfeI GAWTC 5 cut(s) 107, 275, 402, 553, 1051
PkrI GCNGC 7 cut(s) 215, 319, 322, 745, 765, 902, 1048
PpuMI RGGWCCY 2 cut(s) 650, 914
PscI ACATGT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 852
Psp5II RGGWCCY 2 cut(s) 650, 914
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 2 cut(s) 380, 916
PspPI GGNCC 3 cut(s) 379, 650, 914
PspPPI RGGWCCY 2 cut(s) 650, 914
PstI CTGCAG 1 cut(s) 765
RsaI GTAC 3 cut(s) 800, 908, 1088
RsaNI GTAC 3 cut(s) 799, 907, 1087
SacI GAGCTC 1 cut(s) 852
SacII CCGCGG 1 cut(s) 321
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 7 cut(s) 214, 318, 321, 744, 764, 901, 1047
Sau3AI GATC 6 cut(s) 219, 357, 514, 694, 837, 1093
Sau96I GGNCC 3 cut(s) 379, 650, 914
ScaI AGTACT 2 cut(s) 800, 1088
ScrFI CCNGG 2 cut(s) 383, 1081
SduI GDGCHC 2 cut(s) 489, 852
SfaNI GCATC 4 cut(s) 229, 650, 710, 983
SfcI CTRYAG 1 cut(s) 761
Sfr303I CCGCGG 1 cut(s) 321
SfuI TTCGAA 4 cut(s) 110, 536, 588, 866
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 3 cut(s) 379, 650, 914
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
Sse9I AATT 7 cut(s) 27, 112, 422, 584, 842, 859, 1029
SsiI CCGC 7 cut(s) 138, 214, 318, 320, 323, 903, 1097
SspMI CTAG 1 cut(s) 428
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 381, 1079
StyI CCWWGG 2 cut(s) 411, 616
TaaI ACNGT 1 cut(s) 935
TaiI ACGT 1 cut(s) 965
TaqI TCGA 9 cut(s) 110, 225, 356, 498, 536, 588, 701, 836, 866
TasI AATT 7 cut(s) 27, 112, 422, 584, 842, 859, 1029
TatI WGTACW 2 cut(s) 798, 1086
TauI GCSGC 3 cut(s) 216, 320, 323
TfiI GAWTC 5 cut(s) 107, 275, 402, 553, 1051
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 2 cut(s) 935, 979
TseI GCWGC 4 cut(s) 743, 763, 900, 1046
Tsp45I GTSAC 2 cut(s) 935, 979
TspDTI ATGAA 2 cut(s) 758, 1022
TspGWI ACGGA 4 cut(s) 243, 479, 924, 984
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 3 cut(s) 379, 650, 914
XapI RAATTY 5 cut(s) 27, 112, 584, 842, 1029
XceI RCATGY 1 cut(s) 496
XspI CTAG 1 cut(s) 428
ZrmI AGTACT 2 cut(s) 800, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.