pycom13g02050

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002090
Physical Location & Seq
Reverse (-)
1374724 .. 1377396
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGCGTTTCGCGGACTATTTCGGCCGCGCATTCTCTGCGGTGTCTGCCTCTCAGTTTCCATGGGTGAAGATGTTCAAGGAGTCATCGGTGGCCAAGCTTGCCGATATCCCCCTTTCTCATATTTCTGAAGCTGTTTATAAGACATCAGTTGACTGGATCAACCAGCGTTCCGTTGAGGCCCTTGGGTCTTTTATATTATGGTCCTTAGACAGCATTCTTGCAGACCTAGCGAGCCAAGTTTCTGGCACGAAGGGCAAGAAGGCTGTGCAGAATGTGTCTTCAAAATCTCAGGTTGCAATATTTGTGGTTGTAGCAATGGTATTGCGAAGAAAACCCGATGTCTTGATTACTATATTGCCAACACTGAGAGAAAATTCAAAGTATCAAGGACAAGATAAGCTTCCAATAACTATATGGATGATAGTTCAGGCTAGTCAAGGGGACCTCGCCGTTGGGTTGCATGCATGGGCACGCAATGTGTTGCCTTTAGTCTGTGGAAAAAGTAGTAATCCACAGTCCCGAGACCTTGTTTTGCAGCTAGTGGAGAGAATTCTGTCTATGCCCAAAGCTCGTACTATTTTAGTAAATAGTGCTGTTAGGAAGGGGGAGCGTTTGGTGCCACCTTCTGCATTGGAGATACTTATGGGAGTTACCTTCCCTGCACATTCAGCTAGAGTAAAGGCCACTGAAAGGTTTGAGGCTATATATCCAACCCTAAAAGCGGTCGCTCTAGCTGGCACCCCTGGAAGCAAGGCAATGAAACAAGTTTCACAGCATATATTGAGTTTTGCTGTCAAAGCTGCAGGAGAAAGTATACCAGAGCTATCCGATGAAGCAGCTGGAATTTTCATTTGGTGTTTGACCCAACATTCTGATTGCTTTAAGCAGTGGGACAAGGTCTACGAGGAAAACTTAGAAGCGAGTGTTGCTGTTCTGAAGAAACTTTCTAATCAATGGAAAGAACATTCTGCAAATCTCTCTCCTTTTGATCCTATGAGGGAGGCTCTTAAAAGTTTTAGGCGCAAGAATGAGAAGGCATTGGCTGGTGAGGCAGAGGATGCACGCCAAGAAAAGCTCAACAAGGAAGCAGACAAGTATTGTAAGACATTATTGGGGAAGATATCACGGGGAAGCGGGTGCAAGAAAACTGTGGCCCTTGCAGTTGTTGCCTTGGCTGTTGGTGCTGCTGTCATGTCCCCAAACGTGGAGTCTTGGGAATCGGAGTTGAAGAAACTAACAGTGACTATCAGCTCTTTCTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

422

Amino Acids

46.25

Weight (kDa)

9.45

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 102 - 395 2.4e-19 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 616, 737
AccI GTMKAC 2 cut(s) 814, 900
AccII CGCG 2 cut(s) 11, 27
AciI CCGC 5 cut(s) 11, 25, 38, 722, 1134
AclWI GGATC 2 cut(s) 164, 983
AcoI YGGCCR 2 cut(s) 22, 90
AcsI RAATTY 3 cut(s) 373, 549, 843
AcuI CTGAAG 2 cut(s) 147, 956
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 3 cut(s) 690, 721, 1204
AgsI TTSAA 5 cut(s) 76, 282, 378, 1228, 1262
AjnI CCWGG 1 cut(s) 742
AjuI GAANNNNNNNTTGG 2 cut(s) 835, 867
Alw26I GTCTC 1 cut(s) 516
AlwI GGATC 2 cut(s) 164, 983
Ama87I CYCGRG 1 cut(s) 519
AoxI GGCC 5 cut(s) 22, 90, 177, 681, 1152
ApeKI GCWGC 4 cut(s) 535, 800, 836, 1184
ApoI RAATTY 3 cut(s) 373, 549, 843
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 2 cut(s) 29, 1023
AspS9I GGNCC 4 cut(s) 178, 201, 442, 1153
AsuHPI GGTGA 2 cut(s) 76, 1058
AvaI CYCGRG 1 cut(s) 519
AvaII GGWCC 2 cut(s) 201, 442
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 2 cut(s) 616, 737
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 547, 787, 848, 1171
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 516
BfaI CTAG 5 cut(s) 227, 432, 539, 672, 731
BfmI CTRYAG 1 cut(s) 801
BisI GCNGC 5 cut(s) 25, 536, 801, 837, 1185
BlsI GCNGC 5 cut(s) 26, 537, 802, 838, 1186
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 201, 442
BmeT110I CYCGRG 1 cut(s) 519
BmgT120I GGNCC 4 cut(s) 178, 201, 442, 1153
BmiI GGNNCC 3 cut(s) 443, 618, 739
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 1 cut(s) 1048
BpiI GAAGAC 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 988, 1020
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 4 cut(s) 59, 181, 742, 1170
Bsc4I CCNNNNNNNGG 3 cut(s) 690, 721, 1204
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 3 cut(s) 321, 481, 762
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 4 cut(s) 59, 181, 742, 1170
BseGI GGATG 2 cut(s) 423, 1063
BseLI CCNNNNNNNGG 3 cut(s) 690, 721, 1204
BseMI GCAATG 3 cut(s) 321, 481, 762
BseMII CTCAG 3 cut(s) 65, 302, 356
BseNI ACTGG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 472
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 4 cut(s) 547, 787, 848, 1171
BsgI GTGCAG 2 cut(s) 287, 645
Bsh1236I CGCG 2 cut(s) 11, 27
Bsh1285I CGRYCG 2 cut(s) 25, 726
BshFI GGCC 5 cut(s) 24, 92, 179, 683, 1154
BshNI GGYRCC 2 cut(s) 616, 737
BsiEI CGRYCG 2 cut(s) 25, 726
BsiHKCI CYCGRG 1 cut(s) 519
BslFI GGGAC 4 cut(s) 455, 502, 905, 1180
BslI CCNNNNNNNGG 3 cut(s) 690, 721, 1204
BsmAI GTCTC 1 cut(s) 516
BsmFI GGGAC 4 cut(s) 455, 502, 905, 1180
BsmI GAATGC 2 cut(s) 29, 213
BsnI GGCC 5 cut(s) 24, 92, 179, 683, 1154
Bso31I GGTCTC 1 cut(s) 516
BsoBI CYCGRG 1 cut(s) 519
Bsp1286I GDGCHC 1 cut(s) 472
Bsp143I GATC 2 cut(s) 156, 988
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 5 cut(s) 11, 25, 38, 722, 1134
BspANI GGCC 5 cut(s) 24, 92, 179, 683, 1154
BspCNI CTCAG 3 cut(s) 64, 301, 357
BspFNI CGCG 2 cut(s) 11, 27
BspLI GGNNCC 3 cut(s) 443, 618, 739
BspMAI CTGCAG 1 cut(s) 805
BspPI GGATC 2 cut(s) 164, 983
BspT107I GGYRCC 2 cut(s) 616, 737
BspTNI GGTCTC 1 cut(s) 516
BsrDI GCAATG 3 cut(s) 321, 481, 762
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 4 cut(s) 59, 181, 742, 1170
BssMI GATC 2 cut(s) 156, 988
BssNAI GTATAC 1 cut(s) 815
BssT1I CCWWGG 3 cut(s) 59, 181, 1170
Bst1107I GTATAC 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 3 cut(s) 516, 1150, 1240
BstAPI GCANNNNNTGC 3 cut(s) 35, 1058, 1166
BstC8I GCNNGC 6 cut(s) 99, 232, 462, 472, 736, 1063
BstDEI CTNAG 5 cut(s) 51, 205, 288, 365, 913
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 2 cut(s) 423, 1063
BstFNI CGCG 2 cut(s) 11, 27
BstHHI GCGC 2 cut(s) 29, 1023
BstKTI GATC 2 cut(s) 159, 991
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 2 cut(s) 156, 988
BstMCI CGRYCG 2 cut(s) 25, 726
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 2 cut(s) 11, 27
BstV1I GCAGC 4 cut(s) 547, 787, 848, 1171
BstV2I GAAGAC 1 cut(s) 270
BstXI CCANNNNNNTGG 1 cut(s) 242
BstZ17I GTATAC 1 cut(s) 815
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 5 cut(s) 24, 92, 179, 683, 1154
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 2 cut(s) 423, 1063
BtsI GCAGTG 1 cut(s) 893
BtsIMutI CAGTG 4 cut(s) 362, 684, 893, 1245
Cac8I GCNNGC 6 cut(s) 99, 232, 462, 472, 736, 1063
CfoI GCGC 2 cut(s) 29, 1023
Cfr13I GGNCC 4 cut(s) 178, 201, 442, 1153
Csp6I GTAC 1 cut(s) 573
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CviAII CATG 4 cut(s) 60, 461, 465, 1192
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 5 cut(s) 51, 205, 288, 365, 913
DpnI GATC 2 cut(s) 158, 990
DpnII GATC 2 cut(s) 156, 988
EaeI YGGCCR 2 cut(s) 22, 90
EagI CGGCCG 1 cut(s) 22
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 59, 181, 1170
Eco31I GGTCTC 1 cut(s) 516
Eco32I GATATC 2 cut(s) 106, 1122
Eco47I GGWCC 2 cut(s) 201, 442
Eco52I CGGCCG 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 147, 956
Eco88I CYCGRG 1 cut(s) 519
EcoO109I RGGNCCY 2 cut(s) 178, 442
EcoRI GAATTC 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 742
EcoRV GATATC 2 cut(s) 106, 1122
EcoT14I CCWWGG 3 cut(s) 59, 181, 1170
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 3 cut(s) 59, 181, 1170
FaeI CATG 4 cut(s) 63, 464, 468, 1195
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 4 cut(s) 455, 502, 905, 1180
FatI CATG 4 cut(s) 59, 460, 464, 1191
FauI CCCGC 1 cut(s) 1127
FblI GTMKAC 2 cut(s) 814, 900
Fnu4HI GCNGC 5 cut(s) 25, 536, 801, 837, 1185
FokI GGATG 2 cut(s) 430, 1070
Fsp4HI GCNGC 5 cut(s) 25, 536, 801, 837, 1185
FspBI CTAG 5 cut(s) 227, 432, 539, 672, 731
GlaI GCGC 2 cut(s) 28, 1022
GluI GCNGC 5 cut(s) 25, 536, 801, 837, 1185
HaeIII GGCC 5 cut(s) 24, 92, 179, 683, 1154
HhaI GCGC 2 cut(s) 29, 1023
Hin1II CATG 4 cut(s) 63, 464, 468, 1195
Hin6I GCGC 2 cut(s) 27, 1021
HinP1I GCGC 2 cut(s) 27, 1021
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 2 cut(s) 95, 398
HinfI GANTC 3 cut(s) 80, 1208, 1217
HphI GGTGA 2 cut(s) 76, 1058
Hpy166II GTNNAC 3 cut(s) 151, 815, 901
Hpy188I TCNGA 5 cut(s) 127, 829, 874, 936, 1222
Hpy188III TCNNGA 2 cut(s) 343, 519
Hpy8I GTNNAC 3 cut(s) 151, 815, 901
HpyAV CCTTC 6 cut(s) 244, 253, 595, 633, 664, 1027
HpyCH4III ACNGT 3 cut(s) 516, 1150, 1240
HpyCH4IV ACGT 1 cut(s) 1203
HpyF3I CTNAG 5 cut(s) 51, 205, 288, 365, 913
HpySE526I ACGT 1 cut(s) 1203
Hsp92II CATG 4 cut(s) 63, 464, 468, 1195
HspAI GCGC 2 cut(s) 27, 1021
Kzo9I GATC 2 cut(s) 156, 988
LmnI GCTCC 1 cut(s) 607
Lsp1109I GCAGC 4 cut(s) 547, 787, 848, 1171
LweI GCATC 1 cut(s) 1048
MaeI CTAG 5 cut(s) 227, 432, 539, 672, 731
MaeII ACGT 1 cut(s) 1203
MaeIII GTNAC 2 cut(s) 649, 1240
MalI GATC 2 cut(s) 158, 990
MboI GATC 2 cut(s) 156, 988
MboII GAAGA 6 cut(s) 79, 270, 339, 949, 1129, 1240
MhlI GDGCHC 1 cut(s) 472
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 3 cut(s) 373, 549, 843
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 2 cut(s) 89, 1217
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 9 cut(s) 58, 169, 455, 691, 898, 990, 994, 1042, 1048
Mox20I TGGCCA 1 cut(s) 92
Mph1103I ATGCAT 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 71
MscI TGGCCA 1 cut(s) 92
MseI TTAA 2 cut(s) 882, 1008
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 839
MspR9I CCNGG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 29, 213
MvaI CCWGG 1 cut(s) 744
MvnI CGCG 2 cut(s) 11, 27
NcoI CCATGG 1 cut(s) 59
NdeII GATC 2 cut(s) 156, 988
NlaIII CATG 4 cut(s) 63, 464, 468, 1195
NlaIV GGNNCC 3 cut(s) 443, 618, 739
NmuCI GTSAC 1 cut(s) 1240
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 1 cut(s) 464
PaeI GCATGC 1 cut(s) 464
PctI GAATGC 2 cut(s) 29, 213
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 1217
PflFI GACNNNGTC 1 cut(s) 896
PkrI GCNGC 5 cut(s) 26, 537, 802, 838, 1186
PleI GAGTC 2 cut(s) 88, 1216
PpsI GAGTC 2 cut(s) 88, 1216
PpuMI RGGWCCY 1 cut(s) 442
PsiI TTATAA 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 742
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 3 cut(s) 443, 618, 739
PspPI GGNCC 4 cut(s) 178, 201, 442, 1153
PspPPI RGGWCCY 1 cut(s) 442
PstI CTGCAG 1 cut(s) 805
PsyI GACNNNGTC 1 cut(s) 896
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 882, 1008
SatI GCNGC 5 cut(s) 25, 536, 801, 837, 1185
Sau3AI GATC 2 cut(s) 156, 988
Sau96I GGNCC 4 cut(s) 178, 201, 442, 1153
SchI GAGTC 2 cut(s) 89, 1217
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 1 cut(s) 472
SfaNI GCATC 1 cut(s) 1048
SfcI CTRYAG 1 cut(s) 801
SinI GGWCC 2 cut(s) 201, 442
SphI GCATGC 1 cut(s) 464
Sse9I AATT 3 cut(s) 373, 549, 843
SsiI CCGC 5 cut(s) 11, 25, 38, 722, 1134
SspI AATATT 1 cut(s) 300
SspMI CTAG 5 cut(s) 227, 432, 539, 672, 731
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 3 cut(s) 59, 181, 1170
TaaI ACNGT 3 cut(s) 516, 1150, 1240
TaiI ACGT 1 cut(s) 1206
TasI AATT 3 cut(s) 373, 549, 843
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 1217
Tru1I TTAA 2 cut(s) 882, 1008
Tru9I TTAA 2 cut(s) 882, 1008
TscAI CASTG 4 cut(s) 369, 691, 893, 1245
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 4 cut(s) 535, 800, 836, 1184
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 3 cut(s) 773, 838, 846
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 4 cut(s) 369, 691, 893, 1245
Tth111I GACNNNGTC 1 cut(s) 896
VpaK11BI GGWCC 2 cut(s) 201, 442
XapI RAATTY 3 cut(s) 373, 549, 843
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 2 cut(s) 814, 900
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 5 cut(s) 227, 432, 539, 672, 731
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.