pycom13g02910

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004230
Physical Location & Seq
Forward (+)
1992494 .. 1993233
740 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGAGGGTTCAATGTTCATTTACCACTTCAACTATTATTCTCTTTTCTGCGCTGATTTTTCTAGCTTTCATTCATCGAGCGCAAGCAAGAGATCGTAAACCCGTCAATTGTTCTACTTCATGTGGAGAAGTCCAAAACATCAAATACCCTTTTCGTCTAAAGGGCAATCCATCTGGCTGCGGGGATCCTGACCATGAATTTTCTTGCGTGAACGGAAAAACCATCTTAGAAATTTTCCCGGGAAAATACTACGTGCACAACAGTTCATATGATGATCAAAACCTCCGTTTGATCGACGTCAACTTTGCCAACGGATCCTGCAGCCTTCCGTCTGGATCTGTCAAAACAGCGGACGGGAAAGTCATGGATTTTCGGTTCCGAGGAGCTGTAATCTATTATGCTAGTCATTTTCGATTCGTCAAGTGCTCCAAAAACATCAGCAGCCTGCCAACAGCTGCTAATTATCAAGGGTTCCATGTTTGGCCGCAACAAACGGGAGCTATTTCTATGCTGTTTATGTCCCCGAATTATCATCCGGACATGAAGTTCACTTCTTACAAAGCCGTCATGAAACTATTGCAGGCCGGGTTTGATATTGGATGGTCGGTTAAGTGCAGGGACTGCTCTTTGGCTAAGGGATGCGCAGTAAGTTCATGGGTTAAGCCACTTACCTACGAATGCTACACAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

231

Amino Acids

25.7

Weight (kDa)

9.1

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 99 2.7e-16 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AatII GACGTC 1 cut(s) 300
Acc16I TGCGCA 1 cut(s) 643
AccIII TCCGGA 1 cut(s) 535
AciI CCGC 3 cut(s) 180, 350, 485
AclWI GGATC 5 cut(s) 179, 192, 309, 322, 343
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 197, 231
AcyI GRCGYC 1 cut(s) 297
AgsI TTSAA 2 cut(s) 11, 30
AloI GAACNNNNNNTCC 4 cut(s) 359, 391, 530, 562
AluBI AGCT 4 cut(s) 65, 386, 455, 500
AluI AGCT 4 cut(s) 65, 386, 455, 500
Alw21I GWGCWC 2 cut(s) 258, 428
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 5 cut(s) 179, 192, 309, 322, 343
AlwNI CAGNNNCTG 1 cut(s) 621
Ama87I CYCGRG 1 cut(s) 238
Aor13HI TCCGGA 1 cut(s) 535
AoxI GGCC 2 cut(s) 482, 582
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 4 cut(s) 177, 321, 441, 455
ApoI RAATTY 2 cut(s) 197, 231
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspLEI GCGC 3 cut(s) 52, 82, 644
AsuC2I CCSGG 3 cut(s) 239, 240, 586
AvaI CYCGRG 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 2 cut(s) 184, 314
Bbv12I GWGCWC 2 cut(s) 258, 428
BbvI GCAGC 4 cut(s) 164, 333, 442, 453
BccI CCATC 3 cut(s) 178, 230, 594
BceAI ACGGC 1 cut(s) 548
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 3 cut(s) 239, 240, 586
BfaI CTAG 2 cut(s) 62, 402
BfmI CTRYAG 1 cut(s) 319
BisI GCNGC 5 cut(s) 178, 322, 442, 456, 485
BlsI GCNGC 5 cut(s) 179, 323, 443, 457, 486
Bme1390I CCNGG 3 cut(s) 239, 240, 586
BmeT110I CYCGRG 1 cut(s) 238
BmiI GGNNCC 4 cut(s) 186, 316, 377, 473
BmrFI CCNGG 3 cut(s) 239, 240, 586
BmsI GCATC 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 633
BpuMI CCSGG 3 cut(s) 239, 240, 586
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 238, 379
BsaWI WCCGGW 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 238, 379
BseGI GGATG 3 cut(s) 532, 605, 644
BseRI GAGGAG 1 cut(s) 396
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 4 cut(s) 164, 333, 442, 453
BsgI GTGCAG 1 cut(s) 634
BshFI GGCC 2 cut(s) 484, 584
BsiHKAI GWGCWC 2 cut(s) 258, 428
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 3 cut(s) 239, 536, 585
BslFI GGGAC 2 cut(s) 505, 632
BsmFI GGGAC 2 cut(s) 505, 632
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 2 cut(s) 484, 584
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 2 cut(s) 258, 428
Bsp13I TCCGGA 1 cut(s) 535
Bsp143I GATC 6 cut(s) 91, 184, 274, 291, 314, 335
BspACI CCGC 3 cut(s) 180, 350, 485
BspANI GGCC 2 cut(s) 484, 584
BspEI TCCGGA 1 cut(s) 535
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 4 cut(s) 186, 316, 377, 473
BspMAI CTGCAG 1 cut(s) 323
BspPI GGATC 5 cut(s) 179, 192, 309, 322, 343
BssECI CCNNGG 2 cut(s) 238, 379
BssMI GATC 6 cut(s) 91, 184, 274, 291, 314, 335
BssNI GRCGYC 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 621
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 84, 446, 582
BstDEI CTNAG 2 cut(s) 226, 633
BstF5I GGATG 3 cut(s) 532, 605, 644
BstHHI GCGC 3 cut(s) 52, 82, 644
BstKTI GATC 6 cut(s) 94, 187, 277, 294, 317, 338
BstMBI GATC 6 cut(s) 91, 184, 274, 291, 314, 335
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstSCI CCNGG 3 cut(s) 237, 238, 584
BstSFI CTRYAG 1 cut(s) 319
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 4 cut(s) 164, 333, 442, 453
BstX2I RGATCY 3 cut(s) 184, 314, 335
BstYI RGATCY 3 cut(s) 184, 314, 335
BsuRI GGCC 2 cut(s) 484, 584
BtsCI GGATG 3 cut(s) 532, 605, 644
Cac8I GCNNGC 3 cut(s) 84, 446, 582
CaiI CAGNNNCTG 1 cut(s) 621
CciI TCATGA 1 cut(s) 567
CfoI GCGC 3 cut(s) 52, 82, 644
Cfr9I CCCGGG 1 cut(s) 238
CviAII CATG 7 cut(s) 120, 194, 364, 476, 541, 568, 654
DdeI CTNAG 2 cut(s) 226, 633
DpnI GATC 6 cut(s) 93, 186, 276, 293, 316, 337
DpnII GATC 6 cut(s) 91, 184, 274, 291, 314, 335
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 238
FaeI CATG 7 cut(s) 123, 197, 367, 479, 544, 571, 657
FaqI GGGAC 2 cut(s) 505, 632
FatI CATG 7 cut(s) 119, 193, 363, 475, 540, 567, 653
FauI CCCGC 1 cut(s) 173
FauNDI CATATG 1 cut(s) 268
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 5 cut(s) 178, 322, 442, 456, 485
FokI GGATG 3 cut(s) 519, 612, 651
Fsp4HI GCNGC 5 cut(s) 178, 322, 442, 456, 485
FspBI CTAG 2 cut(s) 62, 402
FspI TGCGCA 1 cut(s) 643
GlaI GCGC 3 cut(s) 51, 81, 643
GluI GCNGC 5 cut(s) 178, 322, 442, 456, 485
HaeIII GGCC 2 cut(s) 484, 584
HapII CCGG 3 cut(s) 239, 536, 585
HhaI GCGC 3 cut(s) 52, 82, 644
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 7 cut(s) 123, 197, 367, 479, 544, 571, 657
Hin6I GCGC 3 cut(s) 50, 80, 642
HinP1I GCGC 3 cut(s) 50, 80, 642
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 1 cut(s) 414
HpaII CCGG 3 cut(s) 239, 536, 585
Hpy166II GTNNAC 5 cut(s) 98, 211, 256, 301, 549
Hpy188I TCNGA 1 cut(s) 380
Hpy188III TCNNGA 4 cut(s) 188, 333, 536, 568
Hpy8I GTNNAC 5 cut(s) 98, 211, 256, 301, 549
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 2 cut(s) 252, 297
HpyCH4V TGCA 4 cut(s) 256, 321, 580, 615
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 2 cut(s) 226, 633
HpySE526I ACGT 2 cut(s) 252, 297
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 7 cut(s) 123, 197, 367, 479, 544, 571, 657
HspAI GCGC 3 cut(s) 50, 80, 642
Kpn2I TCCGGA 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 6 cut(s) 91, 184, 274, 291, 314, 335
LmnI GCTCC 3 cut(s) 383, 431, 497
Lsp1109I GCAGC 4 cut(s) 164, 333, 442, 453
LweI GCATC 1 cut(s) 629
MaeI CTAG 2 cut(s) 62, 402
MaeII ACGT 2 cut(s) 252, 297
MalI GATC 6 cut(s) 93, 186, 276, 293, 316, 337
MboI GATC 6 cut(s) 91, 184, 274, 291, 314, 335
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 3 cut(s) 184, 314, 335
MhlI GDGCHC 2 cut(s) 258, 428
MluCI AATT 5 cut(s) 106, 197, 231, 460, 526
MnlI CCTC 2 cut(s) 293, 374
MroI TCCGGA 1 cut(s) 535
MseI TTAA 2 cut(s) 609, 660
MspA1I CMGCKG 2 cut(s) 350, 455
MspI CCGG 3 cut(s) 239, 536, 585
MspR9I CCNGG 3 cut(s) 239, 240, 586
MunI CAATTG 1 cut(s) 106
Mva1269I GAATGC 1 cut(s) 683
MwoI GCNNNNNNNGC 1 cut(s) 621
NciI CCSGG 3 cut(s) 239, 240, 586
NdeI CATATG 1 cut(s) 268
NdeII GATC 6 cut(s) 91, 184, 274, 291, 314, 335
NlaIII CATG 7 cut(s) 123, 197, 367, 479, 544, 571, 657
NlaIV GGNNCC 4 cut(s) 186, 316, 377, 473
NsbI TGCGCA 1 cut(s) 643
PagI TCATGA 1 cut(s) 567
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 5 cut(s) 179, 323, 443, 457, 486
Ppu21I YACGTR 1 cut(s) 253
PspN4I GGNNCC 4 cut(s) 186, 316, 377, 473
PstI CTGCAG 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 621
PsuI RGATCY 3 cut(s) 184, 314, 335
PvuII CAGCTG 1 cut(s) 455
SaqAI TTAA 2 cut(s) 609, 660
SatI GCNGC 5 cut(s) 178, 322, 442, 456, 485
Sau3AI GATC 6 cut(s) 91, 184, 274, 291, 314, 335
ScrFI CCNGG 3 cut(s) 239, 240, 586
SduI GDGCHC 2 cut(s) 258, 428
SetI ASST 9 cut(s) 67, 255, 285, 300, 388, 457, 502, 674, 691
SfaNI GCATC 1 cut(s) 629
SfcI CTRYAG 1 cut(s) 319
SmaI CCCGGG 1 cut(s) 240
Sse9I AATT 5 cut(s) 106, 197, 231, 460, 526
SsiI CCGC 3 cut(s) 180, 350, 485
SspMI CTAG 2 cut(s) 62, 402
StyD4I CCNGG 3 cut(s) 237, 238, 584
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 2 cut(s) 255, 300
TaqI TCGA 3 cut(s) 76, 294, 412
TasI AATT 5 cut(s) 106, 197, 231, 460, 526
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 2 cut(s) 609, 660
Tru9I TTAA 2 cut(s) 609, 660
TseI GCWGC 4 cut(s) 177, 321, 441, 455
TspDTI ATGAA 9 cut(s) 6, 58, 62, 108, 210, 255, 557, 584, 642
TspGWI ACGGA 4 cut(s) 228, 275, 318, 327
TspMI CCCGGG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 254
XapI RAATTY 2 cut(s) 197, 231
XmaI CCCGGG 1 cut(s) 238
XspI CTAG 2 cut(s) 62, 402
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.