pycom13g02920

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004230
Physical Location & Seq
Forward (+)
1995491 .. 1996861
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
ATGGAGATTTCCTTTAAGAAGATGGGCCATGAGCTTCATCCGAAATTATTTACCCAAAGTTCTGAGATTCATAACTTTTCTGCAAAAGCATATAATATAATCATGAGGACGGTCCAATATACATCTTCTCTTCTCTATTCTCTGCTGATTCTGCCATTTTTCGTTCAAATTCAAGCACAAACCAACTGTTCGTCTTCATGCGGAGAAATAAAAAACATCAGCTACCCTTTTCGCCTCAAGGGCGATCCATCCGGCTGCGGCGACTCCGGTTACGAGCTCTCTTGCGTCGATAACAAACCCATCCTAGAGATTTTCCCCGGGAAATACTACGTGCGCAACATTTCCTACTATGATCACACACTCCGTTTGGTCGACGTCAACCTCGCCAATGGAACCTGCGGCCTTCCGTCTGGTCCTGTCGAAACAACGAACGGGTTTGTCCGGGATGCTCGATTCATGGGATATGGGTGGAACCCAGGAAGTCGTTTCCGGTTCGTCAAGTGCTCAACAAACATCAGCAGCGTGCCGGAAGCTGCTAATCACACAGTGGTTCCTTGCCTGACAAGAAACGGGACTTACGTCTATGCTGTTTATGATGGCGATTACTCGTATTATGAGCCTCAAAAATCATGCTCTGTCATTTCGTTGGCTCCTGTCGATCTTCGTAAAGATGCTACGAAGTTCAATTCTTATGAGGCCGTCATGGAACTATTGAAAGCTGGCTTTGAAGTTGGATGGTCAGTTAAATGCAGAGATTGCTCTTTGGCTGGTAAACAATGCGTAGTAAAATCAATGGATAAGCCACTCACCTATATCTGTCAAAAAGAGTACAAAGAATTGACAAGAGATGAAGGAAACTGGATAATTGCTGCAATCGTTGTGGGAGGTCTGATTGCTCTGGGAATTATCATTGGTATAATTGTATTTGTCATCCGAAGATGTCGGAGAAGACGAAATGTAGCTGATAACACGAACAAGAATCCGCAGAATCGAATTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

335

Amino Acids

37.44

Weight (kDa)

8.93

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 63 - 125 2.7e-19 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 378
Acc16I TGCGCA 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 404
AccI GTMKAC 1 cut(s) 372
AciI CCGC 4 cut(s) 201, 258, 399, 983
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 547
AfaI GTAC 1 cut(s) 830
AgsI TTSAA 5 cut(s) 167, 173, 685, 715, 728
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 6 cut(s) 34, 222, 277, 533, 719, 962
AluI AGCT 6 cut(s) 34, 222, 277, 533, 719, 962
Alw21I GWGCWC 2 cut(s) 279, 506
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 3 cut(s) 25, 400, 696
ApeKI GCWGC 4 cut(s) 255, 519, 533, 869
ApoI RAATTY 1 cut(s) 168
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 3 cut(s) 25, 112, 413
AsuC2I CCSGG 3 cut(s) 318, 319, 443
AsuHPI GGTGA 1 cut(s) 799
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 112, 413
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 2 cut(s) 186, 955
Bbv12I GWGCWC 2 cut(s) 279, 506
BbvI GCAGC 4 cut(s) 242, 520, 531, 856
BccI CCATC 5 cut(s) 16, 256, 308, 590, 729
BceAI ACGGC 1 cut(s) 683
BciT130I CCWGG 1 cut(s) 477
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 3 cut(s) 318, 319, 443
BfaI CTAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 240
BisI GCNGC 6 cut(s) 256, 259, 400, 520, 534, 870
BlsI GCNGC 6 cut(s) 257, 260, 401, 521, 535, 871
Bme1390I CCNGG 4 cut(s) 318, 319, 443, 477
Bme18I GGWCC 2 cut(s) 112, 413
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 25, 112, 413
BmiI GGNNCC 4 cut(s) 394, 473, 552, 651
BmrFI CCNGG 4 cut(s) 318, 319, 443, 477
BmsI GCATC 2 cut(s) 436, 661
BoxI GACNNNNGTC 1 cut(s) 578
BpiI GAAGAC 2 cut(s) 186, 955
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 3 cut(s) 318, 319, 443
BsaAI YACGTR 1 cut(s) 331
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 3 cut(s) 316, 317, 475
BsaWI WCCGGW 2 cut(s) 266, 489
Bse1I ACTGG 1 cut(s) 863
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 3 cut(s) 316, 317, 475
BseGI GGATG 6 cut(s) 37, 248, 300, 451, 740, 930
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 863
BseXI GCAGC 4 cut(s) 242, 520, 531, 856
BshFI GGCC 3 cut(s) 27, 402, 698
BsiHKAI GWGCWC 2 cut(s) 279, 506
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 6 cut(s) 252, 267, 318, 442, 490, 527
BslFI GGGAC 1 cut(s) 586
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 3 cut(s) 27, 402, 698
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 279, 506
Bsp143I GATC 3 cut(s) 244, 352, 658
BspACI CCGC 4 cut(s) 201, 258, 399, 983
BspANI GGCC 3 cut(s) 27, 402, 698
BspCNI CTCAG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 4 cut(s) 394, 473, 552, 651
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 239
BsrI ACTGG 1 cut(s) 863
BssECI CCNNGG 3 cut(s) 316, 317, 475
BssMI GATC 3 cut(s) 244, 352, 658
BssNI GRCGYC 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 112, 188, 547
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 756
BstBAI YACGTR 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 524, 721
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 6 cut(s) 37, 248, 300, 451, 740, 930
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 3 cut(s) 247, 355, 661
BstMBI GATC 3 cut(s) 244, 352, 658
BstMWI GCNNNNNNNGC 3 cut(s) 151, 240, 756
BstNI CCWGG 1 cut(s) 477
BstPAI GACNNNNGTC 1 cut(s) 578
BstSCI CCNGG 4 cut(s) 316, 317, 441, 475
BstV1I GCAGC 4 cut(s) 242, 520, 531, 856
BstV2I GAAGAC 2 cut(s) 186, 955
BsuRI GGCC 3 cut(s) 27, 402, 698
BtsCI GGATG 6 cut(s) 37, 248, 300, 451, 740, 930
BtsIMutI CAGTG 1 cut(s) 552
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 524, 721
CciI TCATGA 1 cut(s) 102
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 3 cut(s) 25, 112, 413
Cfr9I CCCGGG 1 cut(s) 317
CseI GACGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 829
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 6 cut(s) 29, 103, 198, 457, 630, 703
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 1 cut(s) 63
DpnI GATC 3 cut(s) 246, 354, 660
DpnII GATC 3 cut(s) 244, 352, 658
DraIII CACNNNGTG 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 112, 413
Eco53kI GAGCTC 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 6 cut(s) 32, 106, 201, 460, 633, 706
FaqI GGGAC 1 cut(s) 586
FatI CATG 6 cut(s) 28, 102, 197, 456, 629, 702
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 6 cut(s) 256, 259, 400, 520, 534, 870
FokI GGATG 6 cut(s) 24, 235, 287, 458, 747, 917
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 6 cut(s) 256, 259, 400, 520, 534, 870
FspBI CTAG 1 cut(s) 305
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 1 cut(s) 335
GluI GCNGC 6 cut(s) 256, 259, 400, 520, 534, 870
HaeIII GGCC 3 cut(s) 27, 402, 698
HapII CCGG 6 cut(s) 252, 267, 318, 442, 490, 527
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 6 cut(s) 32, 106, 201, 460, 633, 706
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 2 cut(s) 373, 379
HindII GTYRAC 2 cut(s) 373, 379
HinfI GANTC 6 cut(s) 67, 148, 263, 453, 979, 988
HpaII CCGG 6 cut(s) 252, 267, 318, 442, 490, 527
HphI GGTGA 1 cut(s) 799
Hpy166II GTNNAC 3 cut(s) 373, 379, 773
Hpy188I TCNGA 5 cut(s) 42, 64, 891, 935, 945
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 3 cut(s) 373, 379, 773
Hpy99I CGWCG 2 cut(s) 290, 377
HpyAV CCTTC 2 cut(s) 413, 845
HpyCH4III ACNGT 3 cut(s) 112, 188, 547
HpyCH4IV ACGT 3 cut(s) 330, 375, 579
HpyCH4V TGCA 3 cut(s) 83, 750, 872
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 240, 756
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 3 cut(s) 330, 375, 579
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 6 cut(s) 32, 106, 201, 460, 633, 706
HspAI GCGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 3 cut(s) 244, 352, 658
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 4 cut(s) 242, 520, 531, 856
LweI GCATC 2 cut(s) 436, 661
MaeI CTAG 1 cut(s) 305
MaeII ACGT 3 cut(s) 330, 375, 579
MaeIII GTNAC 1 cut(s) 269
MalI GATC 3 cut(s) 246, 354, 660
MboI GATC 3 cut(s) 244, 352, 658
MboII GAAGA 7 cut(s) 31, 117, 122, 186, 653, 948, 960
MhlI GDGCHC 2 cut(s) 279, 506
MluCI AATT 8 cut(s) 44, 168, 685, 836, 864, 903, 918, 993
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 2 cut(s) 712, 923
MnlI CCTC 6 cut(s) 99, 245, 392, 630, 688, 878
MseI TTAA 2 cut(s) 15, 744
MspI CCGG 6 cut(s) 252, 267, 318, 442, 490, 527
MspR9I CCNGG 4 cut(s) 318, 319, 443, 477
MvaI CCWGG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 151, 240, 756
NciI CCSGG 3 cut(s) 318, 319, 443
NdeII GATC 3 cut(s) 244, 352, 658
NlaIII CATG 6 cut(s) 32, 106, 201, 460, 633, 706
NlaIV GGNNCC 4 cut(s) 394, 473, 552, 651
NsbI TGCGCA 1 cut(s) 335
PagI TCATGA 1 cut(s) 102
PcsI WCGNNNNNNNCGW 2 cut(s) 576, 949
PfeI GAWTC 5 cut(s) 67, 148, 453, 979, 988
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 6 cut(s) 257, 260, 401, 521, 535, 871
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 578
Psp124BI GAGCTC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 4 cut(s) 394, 473, 552, 651
PspPI GGNCC 3 cut(s) 25, 112, 413
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
SacI GAGCTC 1 cut(s) 279
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 15, 744
SatI GCNGC 6 cut(s) 256, 259, 400, 520, 534, 870
Sau3AI GATC 3 cut(s) 244, 352, 658
Sau96I GGNCC 3 cut(s) 25, 112, 413
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 4 cut(s) 318, 319, 443, 477
SduI GDGCHC 2 cut(s) 279, 506
SfaNI GCATC 2 cut(s) 436, 661
SinI GGWCC 2 cut(s) 112, 413
SmaI CCCGGG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 8 cut(s) 44, 168, 685, 836, 864, 903, 918, 993
SsiI CCGC 4 cut(s) 201, 258, 399, 983
SspMI CTAG 1 cut(s) 305
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 4 cut(s) 316, 317, 441, 475
TaaI ACNGT 3 cut(s) 112, 188, 547
TaiI ACGT 3 cut(s) 333, 378, 582
TaqI TCGA 6 cut(s) 288, 372, 420, 451, 657, 991
TasI AATT 8 cut(s) 44, 168, 685, 836, 864, 903, 918, 993
TatI WGTACW 1 cut(s) 828
TauI GCSGC 2 cut(s) 261, 402
TfiI GAWTC 5 cut(s) 67, 148, 453, 979, 988
Tru1I TTAA 2 cut(s) 15, 744
Tru9I TTAA 2 cut(s) 15, 744
TscAI CASTG 1 cut(s) 552
TseI GCWGC 4 cut(s) 255, 519, 533, 869
TspDTI ATGAA 5 cut(s) 26, 59, 186, 445, 864
TspGWI ACGGA 2 cut(s) 353, 396
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 1 cut(s) 552
VpaK11BI GGWCC 2 cut(s) 112, 413
XapI RAATTY 1 cut(s) 168
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 1 cut(s) 305
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.