pycom13g03540

Aspartate-semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003893
Physical Location & Seq
Forward (+)
2346739 .. 2351818
5080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGGTGGGGGCCGAGCTTGTGTGCAAGCCAATGAGCTTGTCACCATCGGGAAGGAAGCAAGTCGGAGCTCTTAATATAGAGACGACTGCTAAGTTCCAGGATGGGCACAACTCCACCCAATCGCTCATTGCTAACCCAAATTGCTCCACCATTATTTGTTTAATGGCTGTCACCCCTCTCCATCGCCATTCTAAGGTAAAACGAATGGTGGTTAGCACGTATCAGGCTGCTAGTGGAGCTGGTGCTGCTGCCGTGAGAGAGCTTGAGCTGCAAACTAAGGAGGTCCTGGAAGGGAAGCCGCCGACCACTAAGATATTCTCGCGGCAGATCGGTCTATTTTATGCGTTTAATTTGTTTTCGCACAACTCGGCAGTTCTTTCAAATGGATACAATGAAGAGGAAATGAAACTAGTCAAGGAGACAAGAAAAATATGGAGTGACAATGATGTCAAGGTAACTGCCACATGTATACGAGTTCCTGTCACGCGGGCACATGCTGAGAGTATAAATCTTCAGTTTGAGAACCAACTTGATGAAGACACAGCAAGGGAAATTCTGAAGAGATCGCCTGGACTGGTGGTTATTGATGACCGGACAGCGAATAACTTGTTGAGAATCAATGTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230
Pfam Domains
Protein Families
Semialdhyde_dh Semialdhyde_dhC

Protein Analysis

210

Amino Acids

23.09

Weight (kDa)

9.1

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dhC PF02774 58 - 199 3.1e-32 Semialdehyde dehydrogenase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 469
AccII CGCG 2 cut(s) 322, 487
AciI CCGC 3 cut(s) 299, 322, 487
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 2 cut(s) 497, 578
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 464
AgsI TTSAA 1 cut(s) 381
AhlI ACTAGT 1 cut(s) 409
AjnI CCWGG 3 cut(s) 96, 285, 568
AluBI AGCT 6 cut(s) 16, 36, 68, 239, 262, 268
AluI AGCT 6 cut(s) 16, 36, 68, 239, 262, 268
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 74, 413
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 4 cut(s) 227, 245, 248, 268
ApoI RAATTY 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 9, 283
AsuHPI GGTGA 2 cut(s) 33, 163
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 2 cut(s) 108, 493
BanII GRGCYC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 4 cut(s) 214, 232, 235, 255
BccI CCATC 3 cut(s) 52, 95, 189
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 3 cut(s) 98, 287, 570
BciVI GTATCC 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 74, 413
BcuI ACTAGT 1 cut(s) 409
BfaI CTAG 2 cut(s) 231, 410
BfuI GTATCC 1 cut(s) 380
BisI GCNGC 6 cut(s) 228, 246, 249, 269, 299, 323
BlsI GCNGC 6 cut(s) 229, 247, 250, 270, 300, 324
Bme1390I CCNGG 3 cut(s) 98, 287, 570
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 9, 283
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 3 cut(s) 98, 287, 570
BpiI GAAGAC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 284
BsaAI YACGTR 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 591
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 579
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 3 cut(s) 98, 287, 570
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 579
BseSI GKGCMC 2 cut(s) 108, 493
BseXI GCAGC 4 cut(s) 214, 232, 235, 255
Bsh1236I CGCG 2 cut(s) 322, 487
BshFI GGCC 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 592
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 2 cut(s) 74, 413
BsmBI CGTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 70, 108, 493
Bsp143I GATC 2 cut(s) 327, 563
BspACI CCGC 3 cut(s) 299, 322, 487
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 490
BspFNI CGCG 2 cut(s) 322, 487
BspLI GGNNCC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 1 cut(s) 579
BssMI GATC 2 cut(s) 327, 563
BssNAI GTATAC 1 cut(s) 470
Bst1107I GTATAC 1 cut(s) 470
Bst2UI CCWGG 3 cut(s) 98, 287, 570
Bst6I CTCTTC 2 cut(s) 390, 554
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 26, 489
BstDEI CTNAG 5 cut(s) 90, 192, 276, 309, 498
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 2 cut(s) 322, 487
BstKTI GATC 2 cut(s) 330, 566
BstMAI GTCTC 2 cut(s) 74, 413
BstMBI GATC 2 cut(s) 327, 563
BstMWI GCNNNNNNNGC 3 cut(s) 236, 245, 268
BstNI CCWGG 3 cut(s) 98, 287, 570
BstNSI RCATGY 2 cut(s) 468, 497
BstSCI CCNGG 3 cut(s) 96, 285, 568
BstSLI GKGCMC 2 cut(s) 108, 493
BstUI CGCG 2 cut(s) 322, 487
BstV1I GCAGC 4 cut(s) 214, 232, 235, 255
BstV2I GAAGAC 1 cut(s) 543
BstZ17I GTATAC 1 cut(s) 470
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 1 cut(s) 11
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 26, 489
Cfr13I GGNCC 2 cut(s) 9, 283
CviAII CATG 2 cut(s) 465, 494
DdeI CTNAG 5 cut(s) 90, 192, 276, 309, 498
DpnI GATC 2 cut(s) 329, 565
DpnII GATC 2 cut(s) 327, 563
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
Eam1104I CTCTTC 2 cut(s) 390, 554
EarI CTCTTC 2 cut(s) 390, 554
Ecl136II GAGCTC 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 497, 578
EcoICRI GAGCTC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 3 cut(s) 96, 285, 568
EcoT38I GRGCYC 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 2 cut(s) 468, 497
FaiI YATR 7 cut(s) 77, 342, 433, 466, 470, 495, 506
FatI CATG 2 cut(s) 464, 493
FauI CCCGC 1 cut(s) 480
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 6 cut(s) 228, 246, 249, 269, 299, 323
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 6 cut(s) 228, 246, 249, 269, 299, 323
FspBI CTAG 2 cut(s) 231, 410
GluI GCNGC 6 cut(s) 228, 246, 249, 269, 299, 323
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 592
Hin1II CATG 2 cut(s) 468, 497
HinfI GANTC 1 cut(s) 615
HpaII CCGG 1 cut(s) 592
HphI GGTGA 2 cut(s) 33, 163
Hpy166II GTNNAC 1 cut(s) 470
Hpy188I TCNGA 2 cut(s) 65, 558
Hpy188III TCNNGA 1 cut(s) 48
Hpy8I GTNNAC 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 45, 284
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 2 cut(s) 24, 271
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 245, 268
HpyF3I CTNAG 5 cut(s) 90, 192, 276, 309, 498
HpySE526I ACGT 1 cut(s) 218
Hsp92II CATG 2 cut(s) 468, 497
Kzo9I GATC 2 cut(s) 327, 563
LmnI GCTCC 3 cut(s) 65, 149, 236
Lsp1109I GCAGC 4 cut(s) 214, 232, 235, 255
MaeI CTAG 2 cut(s) 231, 410
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 5 cut(s) 39, 169, 437, 454, 481
MalI GATC 2 cut(s) 329, 565
MboI GATC 2 cut(s) 327, 563
MboII GAAGA 4 cut(s) 407, 503, 548, 571
MhlI GDGCHC 3 cut(s) 70, 108, 493
MluCI AATT 3 cut(s) 139, 349, 552
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 3 cut(s) 186, 274, 391
MseI TTAA 3 cut(s) 72, 161, 348
MspI CCGG 1 cut(s) 592
MspR9I CCNGG 3 cut(s) 98, 287, 570
MvaI CCWGG 3 cut(s) 98, 287, 570
MvnI CGCG 2 cut(s) 322, 487
MwoI GCNNNNNNNGC 3 cut(s) 236, 245, 268
NdeII GATC 2 cut(s) 327, 563
NlaIII CATG 2 cut(s) 468, 497
NlaIV GGNNCC 1 cut(s) 10
NmeAIII GCCGAG 2 cut(s) 37, 347
NmuCI GTSAC 4 cut(s) 39, 169, 437, 481
NspI RCATGY 2 cut(s) 468, 497
PciI ACATGT 1 cut(s) 464
PfeI GAWTC 1 cut(s) 615
PfoI TCCNGGA 2 cut(s) 96, 285
PkrI GCNGC 6 cut(s) 229, 247, 250, 270, 300, 324
Ppu21I YACGTR 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 283
PscI ACATGT 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 3 cut(s) 96, 285, 568
PspGI CCWGG 3 cut(s) 96, 285, 568
PspN4I GGNNCC 1 cut(s) 10
PspPI GGNCC 2 cut(s) 9, 283
PspPPI RGGWCCY 1 cut(s) 283
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 3 cut(s) 72, 161, 348
SatI GCNGC 6 cut(s) 228, 246, 249, 269, 299, 323
Sau3AI GATC 2 cut(s) 327, 563
Sau96I GGNCC 2 cut(s) 9, 283
ScrFI CCNGG 3 cut(s) 98, 287, 570
SduI GDGCHC 3 cut(s) 70, 108, 493
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SpeI ACTAGT 1 cut(s) 409
Sse9I AATT 3 cut(s) 139, 349, 552
SsiI CCGC 3 cut(s) 299, 322, 487
SspMI CTAG 2 cut(s) 231, 410
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 3 cut(s) 96, 285, 568
TaiI ACGT 1 cut(s) 221
TaqII GACCGA 1 cut(s) 320
TasI AATT 3 cut(s) 139, 349, 552
TauI GCSGC 2 cut(s) 301, 325
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 3 cut(s) 72, 161, 348
Tru9I TTAA 3 cut(s) 72, 161, 348
TseFI GTSAC 4 cut(s) 39, 169, 437, 481
TseI GCWGC 4 cut(s) 227, 245, 248, 268
Tsp45I GTSAC 4 cut(s) 39, 169, 437, 481
TspDTI ATGAA 3 cut(s) 408, 419, 549
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 552
XceI RCATGY 2 cut(s) 468, 497
XmiI GTMKAC 1 cut(s) 469
XspI CTAG 2 cut(s) 231, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.