pycom13g08640

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003753
Physical Location & Seq
Forward (+)
5733330 .. 5735085
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1218 bp
ATGGACCGTGCAAGTTTTGGTAGTATCAATGGGTGGTGGAGAGGCCCTTGGGGAGTTAAGAAGAGTGCGGAGGCAGTGGCAGTAGGAGTGATTTTAGTTGGTGCCCTTTCGGTTTTCTTAGGCCTTGCACCACAAAACACATCAAGCAATGGTTGCCAATTCCCAGCAATGTTTAACTTTGGCGATTCTAACTCCGACACGGGTGGTTTATCGGCCACATTTTTTCGAGTTCCTGCCCCCTATGGCAATACATTCTTTGGTAAACCTTCAGGCAGGTTTTCAGACGGGCGTCTCATGATCGATTTTATTGCTCAAAAGTTGGGGTTTCCATTTGTGAGTGCTTATCTGGATGCTGTTGGAACTAATTTTTATCATGGAGCAAGTTTTGCGACCGGAGGATCTACCATTCAGCCATTAGATGGCAAGATGTTTGAAGCGAGATATAGCCCCATCTCTCTTAACGTGCAGCTTGCCCAATTTGCGCAGTTGAAAGCACGTGCGAATGAGGGTTTTGCTGAAGGTAAAAGCTCAAAGGTCAGGGCCAGCCTTCCGAGACCAAGCGACTTCTCCAGGGCCTTGTACACATTAGACATTGGACAAAACGATCTTCATGCAGGATTAAGATTGAAGACTCTGGATGAAGTGCTTGCATCCCTTTCCAATATCACTGCACAGTTTGCTTCCACAATAGAGCAACTCTACCAACAAGGAGCAAGAGTTTTTTGGATTCACAACACAGGTCCCCTTGGCTGCTTGCCTGCAAGTCTTGCATATAAACTGCCAGAACCCGGCAACCTGGACCAAAACGGTTGCTTGAAACCTCACAATGAGGTGGCTCAAGAATTTAACCGGAAGCTCAAGGAAAGAGTGCTTAACTTAAGGACCCAACTTTCTGATGCAGTTCTCACTTATGTCGATATTTACACAGCAAAATACAAGCTTATCAATGAGGCAGAGAAGTACGGTTTTTCTAGTCCTCTAGTTCAATGCTGTGGAAGTTACGGTGATCACCATGTCGTCCATTGTGGGATGAAAGCTATTGTTAATGGCACTGAAGTTGAGGCTCTCTGTAGCAATCCTTCGAAGTACATTAGCTGGGATGGGGTGCATTATTCCGATGCTGCAAATGAATGGATAGCCAACCAAATTATGGACGGCTCATTCTCCGACCCTCCAGTATCAATCACTGAAGCGTGTCACAGTCCTCCGCAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

406

Amino Acids

44.0

Weight (kDa)

6.5

Isoelectric Point (pI)

27.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 383 5.2e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
Acc36I ACCTGC 1 cut(s) 264
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 2 cut(s) 419, 1150
AciI CCGC 2 cut(s) 68, 1208
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 4 cut(s) 252, 537, 1074, 1209
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 289
AfaI GTAC 3 cut(s) 581, 962, 1088
AfiI CCNNNNNNNGG 3 cut(s) 419, 788, 1150
AflII CTTAAG 1 cut(s) 877
AgsI TTSAA 5 cut(s) 434, 490, 628, 817, 986
AjnI CCWGG 2 cut(s) 569, 795
AluBI AGCT 6 cut(s) 469, 528, 856, 940, 1037, 1095
AluI AGCT 6 cut(s) 469, 528, 856, 940, 1037, 1095
Alw26I GTCTC 2 cut(s) 296, 547
AlwI GGATC 1 cut(s) 406
AoxI GGCC 5 cut(s) 43, 121, 213, 540, 573
ApeKI GCWGC 3 cut(s) 466, 750, 1121
ApoI RAATTY 1 cut(s) 842
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 7 cut(s) 4, 44, 540, 573, 740, 799, 882
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 2 cut(s) 1001, 1016
AsuII TTCGAA 1 cut(s) 1082
AvaII GGWCC 4 cut(s) 4, 740, 799, 882
BaeGI GKGCMC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 101
BarI GAAGNNNNNNTAC 2 cut(s) 1063, 1095
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 635
BbvI GCAGC 3 cut(s) 478, 737, 1108
BccI CCATC 3 cut(s) 413, 458, 1094
BceAI ACGGC 1 cut(s) 1171
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 593, 627
BciT130I CCWGG 2 cut(s) 571, 797
BclI TGATCA 1 cut(s) 1006
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 296, 547
BfaI CTAG 2 cut(s) 972, 980
BfmI CTRYAG 1 cut(s) 1069
BfrI CTTAAG 1 cut(s) 877
BfuAI ACCTGC 1 cut(s) 264
BisI GCNGC 3 cut(s) 467, 751, 1122
BlsI GCNGC 3 cut(s) 468, 752, 1123
Bme1390I CCNGG 3 cut(s) 571, 789, 797
Bme18I GGWCC 4 cut(s) 4, 740, 799, 882
BmgT120I GGNCC 7 cut(s) 4, 44, 540, 573, 740, 799, 882
BmiI GGNNCC 3 cut(s) 103, 742, 884
BmrFI CCNGG 3 cut(s) 571, 789, 797
BmsI GCATC 4 cut(s) 340, 659, 886, 1108
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 2 cut(s) 553, 1158
Bpu14I TTCGAA 1 cut(s) 1082
BpuEI CTTGAG 2 cut(s) 822, 842
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 289
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 3 cut(s) 47, 570, 745
BsaWI WCCGGW 2 cut(s) 392, 849
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 788, 1150
Bse1I ACTGG 1 cut(s) 1175
Bse3DI GCAATG 2 cut(s) 154, 174
BseBI CCWGG 2 cut(s) 571, 797
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 47, 570, 745
BseGI GGATG 5 cut(s) 355, 643, 650, 1035, 1105
BseLI CCNNNNNNNGG 3 cut(s) 419, 788, 1150
BseMI GCAATG 2 cut(s) 154, 174
BseNI ACTGG 1 cut(s) 1175
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 3 cut(s) 478, 737, 1108
BseYI CCCAGC 2 cut(s) 163, 1095
BsgI GTGCAG 2 cut(s) 485, 654
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 5 cut(s) 45, 123, 215, 542, 575
BshNI GGYRCC 1 cut(s) 101
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 393
BsiSI CCGG 3 cut(s) 393, 789, 850
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 3 cut(s) 419, 788, 1150
BsmAI GTCTC 2 cut(s) 296, 547
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 5 cut(s) 45, 123, 215, 542, 575
Bso31I GGTCTC 1 cut(s) 547
Bsp119I TTCGAA 1 cut(s) 1082
Bsp1286I GDGCHC 1 cut(s) 106
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 4 cut(s) 297, 398, 604, 1006
BspACI CCGC 2 cut(s) 68, 1208
BspANI GGCC 5 cut(s) 45, 123, 215, 542, 575
BspDI ATCGAT 1 cut(s) 300
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 103, 742, 884
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 1 cut(s) 877
BspTNI GGTCTC 1 cut(s) 547
BsrDI GCAATG 2 cut(s) 154, 174
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 1 cut(s) 1175
BssECI CCNNGG 3 cut(s) 47, 570, 745
BssMI GATC 4 cut(s) 297, 398, 604, 1006
BssNI GRCGYC 1 cut(s) 289
BssT1I CCWWGG 2 cut(s) 47, 745
Bst2UI CCWGG 2 cut(s) 571, 797
Bst4CI ACNGT 6 cut(s) 8, 675, 809, 965, 1004, 1202
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 289
BstAFI CTTAAG 1 cut(s) 877
BstAPI GCANNNNNTGC 4 cut(s) 153, 386, 677, 767
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 5 cut(s) 471, 544, 648, 755, 759
BstDEI CTNAG 1 cut(s) 118
BstF5I GGATG 5 cut(s) 355, 643, 650, 1035, 1105
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 4 cut(s) 300, 401, 607, 1009
BstMAI GTCTC 2 cut(s) 296, 547
BstMBI GATC 4 cut(s) 297, 398, 604, 1006
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 5 cut(s) 153, 386, 479, 677, 767
BstNI CCWGG 2 cut(s) 571, 797
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 569, 787, 795
BstSFI CTRYAG 1 cut(s) 1069
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 478, 737, 1108
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 5 cut(s) 45, 123, 215, 542, 575
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 5 cut(s) 355, 643, 650, 1035, 1105
BtsI GCAGTG 2 cut(s) 81, 666
BtsIMutI CAGTG 4 cut(s) 81, 666, 1050, 1185
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 5 cut(s) 471, 544, 648, 755, 759
CciI TCATGA 1 cut(s) 294
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 7 cut(s) 4, 44, 540, 573, 740, 799, 882
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 1 cut(s) 278
Csp6I GTAC 3 cut(s) 580, 961, 1087
CviAII CATG 4 cut(s) 295, 374, 611, 1013
CviQI GTAC 3 cut(s) 580, 961, 1087
DdeI CTNAG 1 cut(s) 118
DpnI GATC 4 cut(s) 299, 400, 606, 1008
DpnII GATC 4 cut(s) 297, 398, 604, 1006
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 47, 745
Eco147I AGGCCT 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 547
Eco47I GGWCC 4 cut(s) 4, 740, 799, 882
Eco57I CTGAAG 4 cut(s) 252, 537, 1074, 1209
Eco72I CACGTG 1 cut(s) 497
EcoO109I RGGNCCY 4 cut(s) 44, 573, 740, 882
EcoRII CCWGG 2 cut(s) 569, 795
EcoT14I CCWWGG 2 cut(s) 47, 745
ErhI CCWWGG 2 cut(s) 47, 745
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 4 cut(s) 298, 377, 614, 1016
FaqI GGGAC 1 cut(s) 726
FatI CATG 4 cut(s) 294, 373, 610, 1012
FbaI TGATCA 1 cut(s) 1006
Fnu4HI GCNGC 3 cut(s) 467, 751, 1122
FokI GGATG 5 cut(s) 362, 637, 650, 1042, 1112
Fsp4HI GCNGC 3 cut(s) 467, 751, 1122
FspBI CTAG 2 cut(s) 972, 980
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 1 cut(s) 483
GluI GCNGC 3 cut(s) 467, 751, 1122
GsaI CCCAGC 2 cut(s) 167, 1099
GsuI CTGGAG 2 cut(s) 553, 1158
HaeIII GGCC 5 cut(s) 45, 123, 215, 542, 575
HapII CCGG 3 cut(s) 393, 789, 850
HgaI GACGC 1 cut(s) 278
HhaI GCGC 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 289
Hin1II CATG 4 cut(s) 298, 377, 614, 1016
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 3 cut(s) 185, 631, 727
HpaII CCGG 3 cut(s) 393, 789, 850
HphI GGTGA 2 cut(s) 1001, 1016
Hpy166II GTNNAC 2 cut(s) 263, 582
Hpy188I TCNGA 7 cut(s) 196, 283, 552, 895, 1117, 1168, 1217
Hpy188III TCNNGA 4 cut(s) 295, 347, 635, 839
Hpy8I GTNNAC 2 cut(s) 263, 582
HpyAV CCTTC 4 cut(s) 276, 512, 557, 1089
HpyCH4III ACNGT 6 cut(s) 8, 675, 809, 965, 1004, 1202
HpyCH4IV ACGT 2 cut(s) 462, 496
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 386, 479, 677, 767
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 2 cut(s) 462, 496
Hsp92I GRCGYC 1 cut(s) 289
Hsp92II CATG 4 cut(s) 298, 377, 614, 1016
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 4 cut(s) 297, 398, 604, 1006
LmnI GCTCC 2 cut(s) 377, 710
Lsp1109I GCAGC 3 cut(s) 478, 737, 1108
LweI GCATC 4 cut(s) 340, 659, 886, 1108
MaeI CTAG 2 cut(s) 972, 980
MaeII ACGT 2 cut(s) 462, 496
MaeIII GTNAC 2 cut(s) 998, 1196
MalI GATC 4 cut(s) 299, 400, 606, 1008
MboI GATC 4 cut(s) 297, 398, 604, 1006
MboII GAAGA 3 cut(s) 73, 599, 640
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 5 cut(s) 158, 364, 476, 842, 1146
MlyI GAGTC 1 cut(s) 625
MmeI TCCRAC 3 cut(s) 219, 337, 1191
MseI TTAA 8 cut(s) 57, 174, 459, 620, 846, 873, 878, 1044
MspCI CTTAAG 1 cut(s) 877
MspI CCGG 3 cut(s) 393, 789, 850
MspR9I CCNGG 3 cut(s) 571, 789, 797
MvaI CCWGG 2 cut(s) 571, 797
MwoI GCNNNNNNNGC 5 cut(s) 153, 386, 479, 677, 767
NciI CCSGG 1 cut(s) 789
NdeII GATC 4 cut(s) 297, 398, 604, 1006
NlaIII CATG 4 cut(s) 298, 377, 614, 1016
NlaIV GGNNCC 3 cut(s) 103, 742, 884
NmuCI GTSAC 1 cut(s) 1196
NsbI TGCGCA 1 cut(s) 483
NspV TTCGAA 1 cut(s) 1082
PagI TCATGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 123
PfeI GAWTC 2 cut(s) 185, 727
PflMI CCANNNNNTGG 2 cut(s) 419, 1150
PkrI GCNGC 3 cut(s) 468, 752, 1123
PleI GAGTC 1 cut(s) 625
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 625
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 2 cut(s) 740, 882
PshAI GACNNNNGTC 1 cut(s) 288
Psp5II RGGWCCY 2 cut(s) 740, 882
Psp6I CCWGG 2 cut(s) 569, 795
PspCI CACGTG 1 cut(s) 497
PspFI CCCAGC 2 cut(s) 163, 1095
PspGI CCWGG 2 cut(s) 569, 795
PspN4I GGNNCC 3 cut(s) 103, 742, 884
PspPI GGNCC 7 cut(s) 4, 44, 540, 573, 740, 799, 882
PspPPI RGGWCCY 2 cut(s) 740, 882
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 3 cut(s) 581, 962, 1088
RsaNI GTAC 3 cut(s) 580, 961, 1087
SaqAI TTAA 8 cut(s) 57, 174, 459, 620, 846, 873, 878, 1044
SatI GCNGC 3 cut(s) 467, 751, 1122
Sau3AI GATC 4 cut(s) 297, 398, 604, 1006
Sau96I GGNCC 7 cut(s) 4, 44, 540, 573, 740, 799, 882
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 3 cut(s) 571, 789, 797
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 4 cut(s) 340, 659, 886, 1108
SfcI CTRYAG 1 cut(s) 1069
SfuI TTCGAA 1 cut(s) 1082
SinI GGWCC 4 cut(s) 4, 740, 799, 882
SmlI CTYRAG 3 cut(s) 837, 857, 877
SmoI CTYRAG 3 cut(s) 837, 857, 877
Sse9I AATT 5 cut(s) 158, 364, 476, 842, 1146
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 2 cut(s) 68, 1208
SspMI CTAG 2 cut(s) 972, 980
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 569, 787, 795
StyI CCWWGG 2 cut(s) 47, 745
TaaI ACNGT 6 cut(s) 8, 675, 809, 965, 1004, 1202
TaiI ACGT 2 cut(s) 465, 499
TaqI TCGA 4 cut(s) 226, 300, 915, 1082
TasI AATT 5 cut(s) 158, 364, 476, 842, 1146
TatI WGTACW 2 cut(s) 579, 1086
TfiI GAWTC 2 cut(s) 185, 727
Tru1I TTAA 8 cut(s) 57, 174, 459, 620, 846, 873, 878, 1044
Tru9I TTAA 8 cut(s) 57, 174, 459, 620, 846, 873, 878, 1044
TscAI CASTG 4 cut(s) 81, 673, 1057, 1192
TseFI GTSAC 1 cut(s) 1196
TseI GCWGC 3 cut(s) 466, 750, 1121
Tsp45I GTSAC 1 cut(s) 1196
TspDTI ATGAA 4 cut(s) 599, 654, 1046, 1143
TspRI CASTG 4 cut(s) 81, 673, 1057, 1192
Van91I CCANNNNNTGG 2 cut(s) 419, 1150
Vha464I CTTAAG 1 cut(s) 877
VpaK11BI GGWCC 4 cut(s) 4, 740, 799, 882
XapI RAATTY 1 cut(s) 842
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XspI CTAG 2 cut(s) 972, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.