pycom13g14820

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002948
Physical Location & Seq
Forward (+)
10731988 .. 10736004
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGTACCGTAATTACCCTCCAATCGTGTCTACACAAAACGAACAAACTAGTTTTCTCTTTTGCCTCGTCAACTGCTTTTCTTCGGTCTCCCTCTACAATCTTACCGCGTTGCCAACCACCACAGCAAGATCTTCGAAACGGTGCGTCGCGATCATACCACGGTTGCACGGTCGCAAAATCCACCTAAATTCGATAGTCTTAGGAGCTCCGAAGCCGCTGCTGGGTGGGGCCATGGAGAAAAGAAGATGGCTGGTATTGGTAATATTAACGTGGATGTTTGTTTTCTCTGCTGGGAGAGAACTGAAAATTAAGCACAACGATCATGGCCCCATATACAATCATACGCTTGCCACTATACTAGTGCGATATGCTTCTACAGTTTATTTGTCAGATTTGTCAGAGCTGTTTACTTGGACATGCTCATGCTGTAATGATTTGATTAAGGGGTTTGAAATGATAGAGATTATTGTTGATGTCCAGCATTGCTTACAGGCTTTTGTTGGAGTGGCACAAGATCCCAATGCTATTATAATTGCATTTAGAGGGACTCAAGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATCTAGATTACCCTGGCATGCCTGATGCAATGGTGCACCACGGGTTTTATAGTGCTTACCACAACACAACCATACGCCCTGGAATTCTAAATGCTGTGGAAATAGCCAGGGAATATTATGGAGATATTGGCATCATAGTTACAGGACATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATCTAAGGGTCAATCAGAAAGAGAAAAATGTTCAAGTTATGACATTTGGACAACCTCGTATTGGTAATGCAGCTTTTGCATCATACTATAGTACACTTGTACCAAACAGTATACGAGTAACAAATGCACATGATATGGTGCCTCATTTACCTCCATACTATACTTATTTTCCTCAGAAGACATACCACCACTTTCCACGAGAGGTGTGGCTTTATAACCTTGAAGTTGGAAGTCTTGTTTATGAAGTTGAGAAAATCTGTGATGAATCAGGCGAGGACCCAACGTGCAGCAGGTCAGTGAGTGGGAACAGCATTTCGGACCATTTAGTTTATTATGGTGTTGACTTAATGGCCAAGACATGGAGACGGTGCAAAATTGTGATCGATACTGGTATAGCAGACTACAGCAGAACAGATCTTGGAGGAAATTTTATACTGTCCAGAGATCTTGCAACTTCTGTTCTCAAGTTGAACGCAGCGCCTGACACTGGGGCAAAACCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

431

Amino Acids

48.36

Weight (kDa)

6.96

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 177 - 315 7.5e-36 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 530, 1002
Acc36I ACCTGC 1 cut(s) 1068
AccB1I GGYRCC 1 cut(s) 925
AccB7I CCANNNNNTGG 1 cut(s) 1146
AccI GTMKAC 2 cut(s) 29, 898
AccII CGCG 2 cut(s) 107, 149
AciI CCGC 2 cut(s) 105, 215
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 1137
AcsI RAATTY 3 cut(s) 187, 684, 1213
AfaI GTAC 3 cut(s) 5, 880, 888
AfiI CCNNNNNNNGG 4 cut(s) 221, 848, 1146, 1274
AgsI TTSAA 6 cut(s) 452, 578, 753, 821, 1010, 1258
AhlI ACTAGT 2 cut(s) 47, 358
AjnI CCWGG 3 cut(s) 613, 679, 707
AluBI AGCT 3 cut(s) 206, 403, 860
AluI AGCT 3 cut(s) 206, 403, 860
Alw21I GWGCWC 3 cut(s) 208, 639, 784
Alw26I GTCTC 2 cut(s) 91, 1144
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 1 cut(s) 509
AlwNI CAGNNNCTG 1 cut(s) 1268
AoxI GGCC 3 cut(s) 228, 325, 1137
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 4 cut(s) 217, 857, 1074, 1262
ApoI RAATTY 3 cut(s) 187, 684, 1213
AspLEI GCGC 1 cut(s) 1267
AspS9I GGNCC 4 cut(s) 228, 326, 1063, 1105
AsuII TTCGAA 1 cut(s) 134
AvaII GGWCC 2 cut(s) 1063, 1105
BaeGI GKGCMC 1 cut(s) 639
BaeI ACNNNNGTAYC 2 cut(s) 870, 903
BalI TGGCCA 1 cut(s) 1139
BanI GGYRCC 1 cut(s) 925
BanII GRGCYC 1 cut(s) 208
BauI CACGAG 1 cut(s) 984
BbsI GAAGAC 2 cut(s) 585, 971
Bbv12I GWGCWC 3 cut(s) 208, 639, 784
BbvI GCAGC 4 cut(s) 204, 869, 1086, 1274
BccI CCATC 1 cut(s) 240
BcgI CGANNNNNNTGC 4 cut(s) 114, 148, 199, 233
BciT130I CCWGG 3 cut(s) 615, 681, 709
BcoDI GTCTC 2 cut(s) 91, 1144
BcuI ACTAGT 2 cut(s) 47, 358
BfaI CTAG 3 cut(s) 48, 359, 605
BfmI CTRYAG 4 cut(s) 375, 874, 1189, 1289
BfoI RGCGCY 1 cut(s) 1268
BfuAI ACCTGC 1 cut(s) 1068
BglII AGATCT 3 cut(s) 128, 1201, 1231
BisI GCNGC 5 cut(s) 215, 218, 858, 1075, 1263
BlsI GCNGC 5 cut(s) 216, 219, 859, 1076, 1264
Bme1390I CCNGG 3 cut(s) 615, 681, 709
Bme18I GGWCC 2 cut(s) 1063, 1105
BmgT120I GGNCC 4 cut(s) 228, 326, 1063, 1105
BmiI GGNNCC 4 cut(s) 229, 328, 927, 1065
BmrFI CCNGG 3 cut(s) 615, 681, 709
BmrI ACTGGG 1 cut(s) 1284
BmsI GCATC 3 cut(s) 616, 741, 875
BmuI ACTGGG 1 cut(s) 1284
BpiI GAAGAC 2 cut(s) 585, 971
Bpu14I TTCGAA 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 534, 1235
Bsa29I ATCGAT 1 cut(s) 1170
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 6 cut(s) 158, 231, 613, 640, 679, 708
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 848, 1146, 1274
Bse1I ACTGG 2 cut(s) 1180, 1279
Bse3DI GCAATG 3 cut(s) 481, 636, 771
BseBI CCWGG 3 cut(s) 615, 681, 709
BseCI ATCGAT 1 cut(s) 1170
BseDI CCNNGG 6 cut(s) 158, 231, 613, 640, 679, 708
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 4 cut(s) 221, 848, 1146, 1274
BseMI GCAATG 3 cut(s) 481, 636, 771
BseMII CTCAG 1 cut(s) 974
BseNI ACTGG 2 cut(s) 1180, 1279
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 4 cut(s) 204, 869, 1086, 1274
BseYI CCCAGC 2 cut(s) 220, 290
BsgI GTGCAG 1 cut(s) 1093
Bsh1236I CGCG 2 cut(s) 107, 149
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 230, 327, 1139
BshNI GGYRCC 1 cut(s) 925
BshVI ATCGAT 1 cut(s) 1170
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 3 cut(s) 208, 639, 784
BslFI GGGAC 1 cut(s) 559
BslI CCNNNNNNNGG 4 cut(s) 221, 848, 1146, 1274
BsmAI GTCTC 2 cut(s) 91, 1144
BsmBI CGTCTC 1 cut(s) 1144
BsmFI GGGAC 1 cut(s) 559
BsnI GGCC 3 cut(s) 230, 327, 1139
Bso31I GGTCTC 1 cut(s) 91
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 3 cut(s) 208, 639, 784
Bsp143I GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
Bsp19I CCATGG 1 cut(s) 231
Bsp68I TCGCGA 1 cut(s) 149
BspACI CCGC 2 cut(s) 105, 215
BspANI GGCC 3 cut(s) 230, 327, 1139
BspCNI CTCAG 1 cut(s) 973
BspDI ATCGAT 1 cut(s) 1170
BspFNI CGCG 2 cut(s) 107, 149
BspLI GGNNCC 4 cut(s) 229, 328, 927, 1065
BspMI ACCTGC 1 cut(s) 1068
BspPI GGATC 1 cut(s) 509
BspT104I TTCGAA 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 925
BspTNI GGTCTC 1 cut(s) 91
BsrDI GCAATG 3 cut(s) 481, 636, 771
BsrI ACTGG 2 cut(s) 1180, 1279
BssECI CCNNGG 6 cut(s) 158, 231, 613, 640, 679, 708
BssMI GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
BssNAI GTATAC 1 cut(s) 899
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 1 cut(s) 231
Bst1107I GTATAC 1 cut(s) 899
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 3 cut(s) 615, 681, 709
Bst4CI ACNGT 8 cut(s) 8, 141, 162, 170, 379, 896, 1155, 1224
BstAPI GCANNNNNTGC 1 cut(s) 863
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 348, 620
BstDEI CTNAG 3 cut(s) 199, 791, 960
BstDSI CCRYGG 3 cut(s) 158, 231, 640
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 2 cut(s) 107, 149
BstH2I RGCGCY 1 cut(s) 1268
BstHHI GCGC 1 cut(s) 1267
BstKTI GATC 9 cut(s) 131, 153, 322, 517, 604, 790, 1170, 1204, 1234
BstMAI GTCTC 2 cut(s) 91, 1144
BstMBI GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 863
BstNI CCWGG 3 cut(s) 615, 681, 709
BstNSI RCATGY 2 cut(s) 420, 622
BstSCI CCNGG 3 cut(s) 613, 679, 707
BstSFI CTRYAG 4 cut(s) 375, 874, 1189, 1289
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 2 cut(s) 107, 149
BstV1I GCAGC 4 cut(s) 204, 869, 1086, 1274
BstV2I GAAGAC 2 cut(s) 585, 971
BstX2I RGATCY 4 cut(s) 128, 514, 1201, 1231
BstYI RGATCY 4 cut(s) 128, 514, 1201, 1231
BstZ17I GTATAC 1 cut(s) 899
Bsu15I ATCGAT 1 cut(s) 1170
BsuRI GGCC 3 cut(s) 230, 327, 1139
BsuTUI ATCGAT 1 cut(s) 1170
BtgI CCRYGG 3 cut(s) 158, 231, 640
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 1089, 1272
BtuMI TCGCGA 1 cut(s) 149
BveI ACCTGC 1 cut(s) 1068
Cac8I GCNNGC 2 cut(s) 348, 620
CaiI CAGNNNCTG 1 cut(s) 1268
CfoI GCGC 1 cut(s) 1267
Cfr13I GGNCC 4 cut(s) 228, 326, 1063, 1105
ClaI ATCGAT 1 cut(s) 1170
CseI GACGC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 4, 879, 887
CviAII CATG 7 cut(s) 232, 323, 417, 423, 619, 917, 1146
CviQI GTAC 3 cut(s) 4, 879, 887
DdeI CTNAG 3 cut(s) 199, 791, 960
DpnI GATC 9 cut(s) 130, 152, 321, 516, 603, 789, 1169, 1203, 1233
DpnII GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
EaeI YGGCCR 1 cut(s) 1137
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 1063, 1105
Eco53kI GAGCTC 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 1063
EcoRI GAATTC 1 cut(s) 684
EcoRII CCWGG 3 cut(s) 613, 679, 707
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 1144
FaeI CATG 7 cut(s) 235, 326, 420, 426, 622, 920, 1149
FaqI GGGAC 1 cut(s) 559
FatI CATG 7 cut(s) 231, 322, 416, 422, 618, 916, 1145
FblI GTMKAC 2 cut(s) 29, 898
Fnu4HI GCNGC 5 cut(s) 215, 218, 858, 1075, 1263
FokI GGATG 1 cut(s) 286
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 5 cut(s) 215, 218, 858, 1075, 1263
FspBI CTAG 3 cut(s) 48, 359, 605
GlaI GCGC 1 cut(s) 1266
GluI GCNGC 5 cut(s) 215, 218, 858, 1075, 1263
GsaI CCCAGC 2 cut(s) 224, 294
HaeII RGCGCY 1 cut(s) 1268
HaeIII GGCC 3 cut(s) 230, 327, 1139
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 1267
Hin1II CATG 7 cut(s) 235, 326, 420, 426, 622, 920, 1149
Hin6I GCGC 1 cut(s) 1265
HinP1I GCGC 1 cut(s) 1265
HincII GTYRAC 2 cut(s) 70, 1129
HindII GTYRAC 2 cut(s) 70, 1129
HinfI GANTC 2 cut(s) 547, 1052
Hpy166II GTNNAC 7 cut(s) 30, 70, 408, 637, 881, 899, 1129
Hpy188I TCNGA 6 cut(s) 210, 391, 400, 804, 963, 1105
Hpy188III TCNNGA 5 cut(s) 148, 551, 605, 785, 1227
Hpy8I GTNNAC 7 cut(s) 30, 70, 408, 637, 881, 899, 1129
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 8 cut(s) 8, 141, 162, 170, 379, 896, 1155, 1224
HpyCH4IV ACGT 2 cut(s) 269, 1070
HpyF10VI GCNNNNNNNGC 1 cut(s) 863
HpyF3I CTNAG 3 cut(s) 199, 791, 960
HpySE526I ACGT 2 cut(s) 269, 1070
Hsp92II CATG 7 cut(s) 235, 326, 420, 426, 622, 920, 1149
HspAI GCGC 1 cut(s) 1265
Kzo9I GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
LmnI GCTCC 2 cut(s) 203, 211
Lsp1109I GCAGC 4 cut(s) 204, 869, 1086, 1274
LweI GCATC 3 cut(s) 616, 741, 875
MaeI CTAG 3 cut(s) 48, 359, 605
MaeII ACGT 2 cut(s) 269, 1070
MaeIII GTNAC 2 cut(s) 739, 904
MalI GATC 9 cut(s) 130, 152, 321, 516, 603, 789, 1169, 1203, 1233
MboI GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
MboII GAAGA 5 cut(s) 72, 123, 255, 590, 976
MflI RGATCY 4 cut(s) 128, 514, 1201, 1231
MhlI GDGCHC 3 cut(s) 208, 639, 784
MlsI TGGCCA 1 cut(s) 1139
MluCI AATT 8 cut(s) 10, 187, 306, 531, 568, 684, 1161, 1213
MluNI TGGCCA 1 cut(s) 1139
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 2 cut(s) 481, 994
Mox20I TGGCCA 1 cut(s) 1139
MscI TGGCCA 1 cut(s) 1139
MseI TTAA 4 cut(s) 266, 309, 441, 1133
MslI CAYNNNNRTG 1 cut(s) 421
Msp20I TGGCCA 1 cut(s) 1139
MspA1I CMGCKG 1 cut(s) 217
MspR9I CCNGG 3 cut(s) 615, 681, 709
MvaI CCWGG 3 cut(s) 615, 681, 709
MvnI CGCG 2 cut(s) 107, 149
MwoI GCNNNNNNNGC 1 cut(s) 863
NcoI CCATGG 1 cut(s) 231
NdeII GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
NlaIII CATG 7 cut(s) 235, 326, 420, 426, 622, 920, 1149
NlaIV GGNNCC 4 cut(s) 229, 328, 927, 1065
NruI TCGCGA 1 cut(s) 149
NspI RCATGY 2 cut(s) 420, 622
NspV TTCGAA 1 cut(s) 134
PaeI GCATGC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 1052
PflMI CCANNNNNTGG 1 cut(s) 1146
PkrI GCNGC 5 cut(s) 216, 219, 859, 1076, 1264
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
PpuMI RGGWCCY 1 cut(s) 1063
PsiI TTATAA 2 cut(s) 530, 1002
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 1063
Psp6I CCWGG 3 cut(s) 613, 679, 707
PspFI CCCAGC 2 cut(s) 220, 290
PspGI CCWGG 3 cut(s) 613, 679, 707
PspN4I GGNNCC 4 cut(s) 229, 328, 927, 1065
PspPI GGNCC 4 cut(s) 228, 326, 1063, 1105
PspPPI RGGWCCY 1 cut(s) 1063
PsrI GAACNNNNNNTAC 2 cut(s) 546, 578
PstNI CAGNNNCTG 1 cut(s) 1268
PsuI RGATCY 4 cut(s) 128, 514, 1201, 1231
RruI TCGCGA 1 cut(s) 149
RsaI GTAC 3 cut(s) 5, 880, 888
RsaNI GTAC 3 cut(s) 4, 879, 887
RseI CAYNNNNRTG 1 cut(s) 421
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 4 cut(s) 266, 309, 441, 1133
SatI GCNGC 5 cut(s) 215, 218, 858, 1075, 1263
Sau3AI GATC 9 cut(s) 128, 150, 319, 514, 601, 787, 1167, 1201, 1231
Sau96I GGNCC 4 cut(s) 228, 326, 1063, 1105
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 3 cut(s) 615, 681, 709
SduI GDGCHC 3 cut(s) 208, 639, 784
SfaNI GCATC 3 cut(s) 616, 741, 875
SfcI CTRYAG 4 cut(s) 375, 874, 1189, 1289
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 2 cut(s) 1063, 1105
SmiMI CAYNNNNRTG 1 cut(s) 421
SmlI CTYRAG 2 cut(s) 549, 1250
SmoI CTYRAG 2 cut(s) 549, 1250
SpeI ACTAGT 2 cut(s) 47, 358
SphI GCATGC 1 cut(s) 622
Sse9I AATT 8 cut(s) 10, 187, 306, 531, 568, 684, 1161, 1213
SsiI CCGC 2 cut(s) 105, 215
SspI AATATT 2 cut(s) 264, 716
SspMI CTAG 3 cut(s) 48, 359, 605
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 3 cut(s) 613, 679, 707
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 8 cut(s) 8, 141, 162, 170, 379, 896, 1155, 1224
TaiI ACGT 2 cut(s) 272, 1073
TaqI TCGA 3 cut(s) 134, 191, 1170
TaqII GACCGA 1 cut(s) 73
TasI AATT 8 cut(s) 10, 187, 306, 531, 568, 684, 1161, 1213
TatI WGTACW 1 cut(s) 878
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 1 cut(s) 1052
Tru1I TTAA 4 cut(s) 266, 309, 441, 1133
Tru9I TTAA 4 cut(s) 266, 309, 441, 1133
TscAI CASTG 2 cut(s) 1089, 1279
TseI GCWGC 4 cut(s) 217, 857, 1074, 1262
TspDTI ATGAA 3 cut(s) 762, 1044, 1065
TspRI CASTG 2 cut(s) 1089, 1279
Van91I CCANNNNNTGG 1 cut(s) 1146
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 1063, 1105
XapI RAATTY 3 cut(s) 187, 684, 1213
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 2 cut(s) 420, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 990
XmiI GTMKAC 2 cut(s) 29, 898
XspI CTAG 3 cut(s) 48, 359, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.