pycom13g15500

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007530
Physical Location & Seq
Reverse (-)
11303920 .. 11304576
657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
ATGGCTTCCCTGTTTACCGAGTCTTGGTCTAAAGTTGTCATTATCTTCCTACTGATTCTCCCATTTTTGGTTTCAAGGCACCTTCTGATCGTGCGCCGGGCGCATTGTGGTTTACTGGGGCCAAAATGGAGGGAGGGCGCACTAACAGCAACATGTAACACAGGAAGATACCGGATTGTAAACATAGCATTCCTCTCACAGTTTGGCAATGGCCAAACCCCTCAAATCAACTTAGCCGGCCACTGTGATCCGGCGTCTAACGGCTGTCAAAAAGTGAGCACCGGCATCAGAAACTGCCAGAACCAAGGCATTAAGGTCCTGCTTTCGATTGGTGGCAGCTTGGGGAGTTACACGCTATCATCTGCAGATGATGCTAGAGGTGTAGCGGACTACATATGGAATAACTTCTTAGGGGGGCAATCAAACTCGAGGCCTCTAGGAGACGCCGTATTGGATGGCGTGGACTTCGATATCGAGAGAGGCAGAAAAGTGTCCTTAAGTGCAGCACCGCAATGTCCGTTTCCTGACCAGAAACTCAATGGTGCACTAACCACTGGCCTATTTGACTATGTTTGGGTTCAATTCTACAATAATCCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

201

Amino Acids

21.72

Weight (kDa)

8.81

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 52 - 159 1.3e-08 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 78
AciI CCGC 2 cut(s) 386, 509
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 2 cut(s) 211, 238
AcyI GRCGYC 2 cut(s) 254, 444
AfiI CCNNNNNNNGG 2 cut(s) 24, 67
AflII CTTAAG 1 cut(s) 496
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 2 cut(s) 75, 581
AluBI AGCT 1 cut(s) 339
AluI AGCT 1 cut(s) 339
Alw21I GWGCWC 2 cut(s) 281, 547
Alw26I GTCTC 1 cut(s) 435
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 1 cut(s) 294
Ama87I CYCGRG 1 cut(s) 427
AoxI GGCC 5 cut(s) 119, 211, 238, 431, 556
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 2 cut(s) 336, 503
Asp700I GAANNNNTTC 1 cut(s) 404
AspLEI GCGC 3 cut(s) 96, 103, 140
AspS9I GGNCC 2 cut(s) 119, 316
AsuC2I CCSGG 1 cut(s) 98
AvaI CYCGRG 1 cut(s) 427
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 547
BalI TGGCCA 1 cut(s) 213
BanI GGYRCC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 281, 547
BbvI GCAGC 2 cut(s) 348, 515
BccI CCATC 1 cut(s) 449
BceAI ACGGC 2 cut(s) 277, 431
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 375, 437
BfmI CTRYAG 1 cut(s) 363
BfrI CTTAAG 1 cut(s) 496
BisI GCNGC 2 cut(s) 337, 504
BlsI GCNGC 2 cut(s) 338, 505
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 119, 316
BmiI GGNNCC 2 cut(s) 80, 120
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 294, 361
BmuI ACTGGG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 98
BsaHI GRCGYC 2 cut(s) 254, 444
BsaJI CCNNGG 1 cut(s) 304
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 432, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 67
Bse118I RCCGGY 2 cut(s) 236, 281
Bse1I ACTGG 3 cut(s) 120, 559, 596
Bse3DI GCAATG 2 cut(s) 214, 518
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 24, 67
BseMI GCAATG 2 cut(s) 214, 518
BseNI ACTGG 3 cut(s) 120, 559, 596
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 2 cut(s) 348, 515
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 5 cut(s) 121, 213, 240, 433, 558
BshNI GGYRCC 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 281, 547
BsiHKCI CYCGRG 1 cut(s) 427
BsiSI CCGG 5 cut(s) 97, 172, 237, 251, 282
BslI CCNNNNNNNGG 2 cut(s) 24, 67
BsmAI GTCTC 1 cut(s) 435
BsmBI CGTCTC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 5 cut(s) 121, 213, 240, 433, 558
BsoBI CYCGRG 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 281, 547
Bsp143I GATC 2 cut(s) 87, 247
BspACI CCGC 2 cut(s) 386, 509
BspANI GGCC 5 cut(s) 121, 213, 240, 433, 558
BspLI GGNNCC 2 cut(s) 80, 120
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 78
BspTI CTTAAG 1 cut(s) 496
BsrDI GCAATG 2 cut(s) 214, 518
BsrFI RCCGGY 2 cut(s) 236, 281
BsrI ACTGG 3 cut(s) 120, 559, 596
BssAI RCCGGY 2 cut(s) 236, 281
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 2 cut(s) 87, 247
BssNI GRCGYC 2 cut(s) 254, 444
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 201, 245
BstACI GRCGYC 2 cut(s) 254, 444
BstAFI CTTAAG 1 cut(s) 496
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 232, 409
BstF5I GGATG 1 cut(s) 460
BstHHI GCGC 3 cut(s) 96, 103, 140
BstKTI GATC 2 cut(s) 90, 250
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 2 cut(s) 87, 247
BstMWI GCNNNNNNNGC 3 cut(s) 100, 146, 371
BstNSI RCATGY 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 2 cut(s) 348, 515
BsuRI GGCC 5 cut(s) 121, 213, 240, 433, 558
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 3 cut(s) 241, 552, 603
Cac8I GCNNGC 1 cut(s) 238
CaiI CAGNNNCTG 1 cut(s) 294
CfoI GCGC 3 cut(s) 96, 103, 140
Cfr10I RCCGGY 2 cut(s) 236, 281
Cfr13I GGNCC 2 cut(s) 119, 316
CseI GACGC 2 cut(s) 243, 452
CviAII CATG 1 cut(s) 153
CviJI RGCY 9 cut(s) 5, 121, 213, 236, 240, 264, 339, 433, 558
CviKI_1 RGCY 9 cut(s) 5, 121, 213, 236, 240, 264, 339, 433, 558
DdeI CTNAG 2 cut(s) 232, 409
DpnI GATC 2 cut(s) 89, 249
DpnII GATC 2 cut(s) 87, 247
EaeI YGGCCR 2 cut(s) 211, 238
Eco130I CCWWGG 1 cut(s) 304
Eco147I AGGCCT 1 cut(s) 433
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 316
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 304
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 1 cut(s) 156
FaiI YATR 5 cut(s) 154, 185, 395, 397, 570
FatI CATG 1 cut(s) 152
FauNDI CATATG 1 cut(s) 395
Fnu4HI GCNGC 2 cut(s) 337, 504
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 2 cut(s) 337, 504
FspBI CTAG 2 cut(s) 375, 437
GlaI GCGC 3 cut(s) 95, 102, 139
GluI GCNGC 2 cut(s) 337, 504
HaeIII GGCC 5 cut(s) 121, 213, 240, 433, 558
HapII CCGG 5 cut(s) 97, 172, 237, 251, 282
HgaI GACGC 2 cut(s) 243, 452
HhaI GCGC 3 cut(s) 96, 103, 140
Hin1I GRCGYC 2 cut(s) 254, 444
Hin1II CATG 1 cut(s) 156
Hin6I GCGC 3 cut(s) 94, 101, 138
HinP1I GCGC 3 cut(s) 94, 101, 138
HinfI GANTC 2 cut(s) 20, 55
HpaII CCGG 5 cut(s) 97, 172, 237, 251, 282
Hpy166II GTNNAC 5 cut(s) 15, 113, 181, 463, 545
Hpy188I TCNGA 2 cut(s) 87, 290
Hpy188III TCNNGA 2 cut(s) 475, 524
Hpy8I GTNNAC 5 cut(s) 15, 113, 181, 463, 545
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 2 cut(s) 201, 245
HpyCH4V TGCA 3 cut(s) 365, 503, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 146, 371
HpyF3I CTNAG 2 cut(s) 232, 409
Hsp92I GRCGYC 2 cut(s) 254, 444
Hsp92II CATG 1 cut(s) 156
HspAI GCGC 3 cut(s) 94, 101, 138
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 2 cut(s) 87, 247
Lsp1109I GCAGC 2 cut(s) 348, 515
LweI GCATC 2 cut(s) 294, 361
MaeI CTAG 2 cut(s) 375, 437
MaeIII GTNAC 2 cut(s) 155, 347
MalI GATC 2 cut(s) 89, 249
MboI GATC 2 cut(s) 87, 247
MboII GAAGA 2 cut(s) 37, 177
MhlI GDGCHC 2 cut(s) 281, 547
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 1 cut(s) 581
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 8 cut(s) 123, 127, 203, 231, 371, 423, 444, 473
Mox20I TGGCCA 1 cut(s) 213
MroNI GCCGGC 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 404
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 312, 497
MslI CAYNNNNRTG 1 cut(s) 511
Msp20I TGGCCA 1 cut(s) 213
MspCI CTTAAG 1 cut(s) 496
MspI CCGG 5 cut(s) 97, 172, 237, 251, 282
MspR9I CCNGG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 100, 146, 371
NaeI GCCGGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 98
NdeI CATATG 1 cut(s) 395
NdeII GATC 2 cut(s) 87, 247
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 1 cut(s) 156
NlaIV GGNNCC 2 cut(s) 80, 120
NspI RCATGY 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 427
PceI AGGCCT 1 cut(s) 433
PciI ACATGT 1 cut(s) 152
PctI GAATGC 1 cut(s) 188
PdiI GCCGGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 55
PkrI GCNGC 2 cut(s) 338, 505
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 80, 120
PspPI GGNCC 2 cut(s) 119, 316
PspPPI RGGWCCY 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 427
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 294
RseI CAYNNNNRTG 1 cut(s) 511
SaqAI TTAA 2 cut(s) 312, 497
SatI GCNGC 2 cut(s) 337, 504
Sau3AI GATC 2 cut(s) 87, 247
Sau96I GGNCC 2 cut(s) 119, 316
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 2 cut(s) 281, 547
SetI ASST 4 cut(s) 84, 318, 341, 382
SfaNI GCATC 2 cut(s) 294, 361
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 427
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 511
SmlI CTYRAG 2 cut(s) 427, 496
SmoI CTYRAG 2 cut(s) 427, 496
Sse9I AATT 1 cut(s) 581
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 2 cut(s) 386, 509
SspMI CTAG 2 cut(s) 375, 437
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 201, 245
TaqI TCGA 4 cut(s) 326, 428, 468, 474
TasI AATT 1 cut(s) 581
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 2 cut(s) 312, 497
Tru9I TTAA 2 cut(s) 312, 497
TscAI CASTG 3 cut(s) 248, 559, 603
TseI GCWGC 2 cut(s) 336, 503
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 3 cut(s) 248, 559, 603
Vha464I CTTAAG 1 cut(s) 496
VneI GTGCAC 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 1 cut(s) 156
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XhoI CTCGAG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 2 cut(s) 375, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.