pycom13g20610

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004128
Physical Location & Seq
Reverse (-)
16771514 .. 16778447
6934 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1974 bp
ATGCCCATTTTACATTGCGACACATGTCGTTCATTTATATTTACTTATATCGTAACACATTACACACGACTTGCTAATCCACGTGTTAGTGATTGTTATGAGGACTTACTAGCGAGTGTTTTAAGTGCACTTTCACGGCATCGGAACACCCTCTCCTCCGTCAGCCGTCCTCCACTCCTCTTCCTCCCTTCACCGCCCAGCTCGGCCCACACTCCTCCTTCTTCTTCATTGGCAATTTGGCATTGTCAACAGCAACATCAATATTTGGTTTTGTTTATTTGGTGTAACTCAGGTCGCTTTGTTTCGAAAATGGACGCCACAATGCCCATCTGCAAGACTGTCACGCCAACTCCGGGCTTATTTTTTGGGAGAACTAGGGAGATCCGTAGTTCTCAATGTAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGTTCAGGGTGCTCAAGTTAGTGTTAAATCCAGAAAGCATGGGGGAGCTCTTCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAATAGAGGGGAAATTGCTGTTCGCGTTATTCGAACAGCCCATGAGATGGGAATACCTTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTGAAATTGGCTGACGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACTTAGTTGTTCCAAATGTTTTATCTGCTGCCATCAGTTGGAAATGTACAATGTTGCATCCAGGATATGGTTTCCTTTCTGAGAATGCATCATTTGTTGAAATGTGCAAAGAACATGGAATCAATTTTATTGGGCCTAAGCCCGAGAGCATCCGTGTCATGGGTGATAAATCAACTGCCAGAGACACAATGAAGAAAGCAGGTGTCCCAACTGTACCTGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCAGATGAGATTGGTTTTCCTGTAATGATCAAGGCCACAGCAGGAGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGTTGTTGCAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAGTTTCAGGTTCTTGCAGATAAATTTGGTAATGTTATTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTTCTGGAAGAAGCACCTTCCCCTGCATTGACCCCAGAACTGCGGAAAGCTATGGGTGATGCAGCAGTTGCAGCAGCAGCATCTATTGGCTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTGGCTATGGGTGAAAAACTCCGATACACGCAGGAAGATATAGTGCTCAGAGGACATTCAATTGAATGCCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTCGAATGGATAGCCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCGATTGAGCGAATGAAGAGGGCTCTTGACGACACTGTTATAACAGGGGTTCCCACAACCATTGAATACCATAAACTTATCCTTGACGTAGAGGATTTCAAAAATGGCAAGGTTGATACTGCTTTTATTCCAAAGCATGAAGAGGAGCTACAAGCGGTAAGAAACTACCCCAACATTTGGTGCCCGCGGCCGCTGCCAAGAACTAGCTGGTGCATCTGCTTACATGGATGGCTCTCTAATTGCCACTATGTATCTGGTCTAGTCGACCACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

658

Amino Acids

72.96

Weight (kDa)

8.03

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 171 - 278 3e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 283 - 490 2.7e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 305 - 460 1.5e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 314 - 459 3.3e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 504 - 610 2.5e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1739
AarI CACCTGC 1 cut(s) 872
Acc36I ACCTGC 1 cut(s) 872
AccB1I GGYRCC 2 cut(s) 1681, 1878
AccB7I CCANNNNNTGG 4 cut(s) 574, 720, 749, 1875
AccBSI CCGCTC 1 cut(s) 1183
AccI GTMKAC 1 cut(s) 1962
AccII CGCG 2 cut(s) 552, 1885
AciI CCGC 7 cut(s) 194, 1183, 1258, 1853, 1883, 1885, 1889
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 1886
AcsI RAATTY 4 cut(s) 516, 1154, 1328, 1440
AcvI CACGTG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 4 cut(s) 683, 730, 897, 1109
AfiI CCNNNNNNNGG 8 cut(s) 353, 574, 720, 749, 841, 1559, 1589, 1875
AflIII ACRYGT 3 cut(s) 23, 82, 500
AgsI TTSAA 7 cut(s) 782, 1328, 1433, 1506, 1511, 1763, 1798
AjiI CACGTC 1 cut(s) 1001
AjnI CCWGG 4 cut(s) 742, 898, 1552, 1558
AjuI GAANNNNNNNTTGG 2 cut(s) 1636, 1668
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 9 cut(s) 201, 402, 488, 1073, 1216, 1265, 1669, 1846, 1905
AluI AGCT 9 cut(s) 201, 402, 488, 1073, 1216, 1265, 1669, 1846, 1905
Alw21I GWGCWC 5 cut(s) 130, 454, 490, 926, 1494
Alw26I GTCTC 1 cut(s) 858
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 5 cut(s) 204, 815, 978, 1556, 1886
ApaLI GTGCAC 1 cut(s) 126
ApoI RAATTY 4 cut(s) 516, 1154, 1328, 1440
Asp700I GAANNNNTTC 2 cut(s) 516, 1440
AspS9I GGNCC 4 cut(s) 205, 815, 1556, 1592
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 5 cut(s) 183, 671, 857, 1283, 1469
AsuII TTCGAA 3 cut(s) 305, 559, 1603
AvaI CYCGRG 1 cut(s) 824
AvaII GGWCC 1 cut(s) 1592
AxyI CCTNAGG 1 cut(s) 1026
BaeGI GKGCMC 3 cut(s) 130, 1684, 1883
BanI GGYRCC 2 cut(s) 1681, 1878
BanII GRGCYC 2 cut(s) 490, 1723
BarI GAAGNNNNNNTAC 2 cut(s) 1830, 1862
BbrPI CACGTG 1 cut(s) 83
Bbv12I GWGCWC 5 cut(s) 130, 454, 490, 926, 1494
BccI CCATC 7 cut(s) 335, 568, 722, 902, 1082, 1591, 1920
BceAI ACGGC 3 cut(s) 150, 152, 1500
BciT130I CCWGG 4 cut(s) 744, 900, 1554, 1560
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 5 cut(s) 110, 375, 1122, 1902, 1958
BfmI CTRYAG 2 cut(s) 917, 1068
BfuAI ACCTGC 1 cut(s) 872
Bme1390I CCNGG 5 cut(s) 354, 744, 900, 1554, 1560
Bme18I GGWCC 1 cut(s) 1592
BmeT110I CYCGRG 1 cut(s) 824
BmgBI CACGTC 1 cut(s) 1001
BmgT120I GGNCC 4 cut(s) 205, 815, 1556, 1592
BmiI GGNNCC 8 cut(s) 1208, 1321, 1351, 1594, 1638, 1683, 1749, 1880
BmrFI CCNGG 5 cut(s) 354, 744, 900, 1554, 1560
BoxI GACNNNNGTC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 1608
Bpu10I CCTNAGC 1 cut(s) 819
Bpu14I TTCGAA 3 cut(s) 305, 559, 1603
BpuEI CTTGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 1553, 1883
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 334, 364
Bsc4I CCNNNNNNNGG 8 cut(s) 353, 574, 720, 749, 841, 1559, 1589, 1875
Bse21I CCTNAGG 1 cut(s) 1026
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 4 cut(s) 744, 900, 1554, 1560
BseDI CCNNGG 2 cut(s) 1553, 1883
BseGI GGATG 7 cut(s) 622, 739, 831, 1110, 1194, 1393, 1931
BseLI CCNNNNNNNGG 8 cut(s) 353, 574, 720, 749, 841, 1559, 1589, 1875
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 4 cut(s) 303, 753, 1017, 1507
BseRI GAGGAG 5 cut(s) 145, 167, 204, 1043, 1856
BseSI GKGCMC 3 cut(s) 130, 1684, 1883
BseX3I CGGCCG 1 cut(s) 1886
BseYI CCCAGC 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 552, 1885
Bsh1285I CGRYCG 1 cut(s) 1889
BshFI GGCC 5 cut(s) 206, 817, 980, 1558, 1888
BshNI GGYRCC 2 cut(s) 1681, 1878
BsiEI CGRYCG 1 cut(s) 1889
BsiHKAI GWGCWC 5 cut(s) 130, 454, 490, 926, 1494
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 2 cut(s) 872, 1578
BslI CCNNNNNNNGG 8 cut(s) 353, 574, 720, 749, 841, 1559, 1589, 1875
BsmAI GTCTC 1 cut(s) 858
BsmFI GGGAC 2 cut(s) 872, 1578
BsmI GAATGC 2 cut(s) 772, 1517
BsnI GGCC 5 cut(s) 206, 817, 980, 1558, 1888
BsoBI CYCGRG 1 cut(s) 824
Bsp119I TTCGAA 3 cut(s) 305, 559, 1603
Bsp1286I GDGCHC 8 cut(s) 130, 454, 490, 926, 1494, 1684, 1723, 1883
Bsp1407I TGTACA 1 cut(s) 728
Bsp143I GATC 2 cut(s) 381, 972
BspACI CCGC 7 cut(s) 194, 1183, 1258, 1853, 1883, 1885, 1889
BspANI GGCC 5 cut(s) 206, 817, 980, 1558, 1888
BspCNI CTCAG 4 cut(s) 302, 754, 1018, 1506
BspFNI CGCG 2 cut(s) 552, 1885
BspLI GGNNCC 8 cut(s) 1208, 1321, 1351, 1594, 1638, 1683, 1749, 1880
BspMAI CTGCAG 1 cut(s) 1072
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 495
BspT104I TTCGAA 3 cut(s) 305, 559, 1603
BspT107I GGYRCC 2 cut(s) 1681, 1878
BsrBI CCGCTC 1 cut(s) 1183
BsrDI GCAATG 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 728
BssECI CCNNGG 2 cut(s) 1553, 1883
BssMI GATC 2 cut(s) 381, 972
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 4 cut(s) 744, 900, 1554, 1560
Bst4CI ACNGT 4 cut(s) 340, 895, 1324, 1735
Bst6I CTCTTC 4 cut(s) 185, 495, 1709, 1833
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 2 cut(s) 1283, 1532
BstAUI TGTACA 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 83
BstBI TTCGAA 3 cut(s) 305, 559, 1603
BstC8I GCNNGC 5 cut(s) 670, 945, 1007, 1053, 1883
BstDEI CTNAG 6 cut(s) 289, 685, 762, 819, 1026, 1493
BstDSI CCRYGG 1 cut(s) 1883
BstENI CCTNNNNNAGG 1 cut(s) 1557
BstF5I GGATG 7 cut(s) 622, 739, 831, 1110, 1194, 1393, 1931
BstFNI CGCG 2 cut(s) 552, 1885
BstKTI GATC 2 cut(s) 384, 975
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 2 cut(s) 381, 972
BstMCI CGRYCG 1 cut(s) 1889
BstMWI GCNNNNNNNGC 7 cut(s) 494, 1073, 1283, 1286, 1292, 1532, 1891
BstNI CCWGG 4 cut(s) 744, 900, 1554, 1560
BstNSI RCATGY 3 cut(s) 27, 504, 1009
BstPAI GACNNNNGTC 1 cut(s) 24
BstSCI CCNGG 5 cut(s) 352, 742, 898, 1552, 1558
BstSFI CTRYAG 2 cut(s) 917, 1068
BstSLI GKGCMC 3 cut(s) 130, 1684, 1883
BstUI CGCG 2 cut(s) 552, 1885
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BstZI CGGCCG 1 cut(s) 1886
Bsu36I CCTNAGG 1 cut(s) 1026
BsuRI GGCC 5 cut(s) 206, 817, 980, 1558, 1888
BtgI CCRYGG 1 cut(s) 1883
BtrI CACGTC 1 cut(s) 1001
BtsCI GGATG 7 cut(s) 622, 739, 831, 1110, 1194, 1393, 1931
BtsIMutI CAGTG 1 cut(s) 1731
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 5 cut(s) 670, 945, 1007, 1053, 1883
CciNI GCGGCCGC 1 cut(s) 1886
Cfr13I GGNCC 4 cut(s) 205, 815, 1556, 1592
Cfr42I CCGCGG 1 cut(s) 1886
CseI GACGC 2 cut(s) 323, 998
Csp6I GTAC 4 cut(s) 682, 729, 896, 1108
CspCI CAANNNNNGTGG 2 cut(s) 1741, 1776
CviQI GTAC 4 cut(s) 682, 729, 896, 1108
DdeI CTNAG 6 cut(s) 289, 685, 762, 819, 1026, 1493
DpnI GATC 2 cut(s) 383, 974
DpnII GATC 2 cut(s) 381, 972
DraI TTTAAA 1 cut(s) 1540
EaeI YGGCCR 1 cut(s) 1886
EagI CGGCCG 1 cut(s) 1886
Eam1104I CTCTTC 4 cut(s) 185, 495, 1709, 1833
EarI CTCTTC 4 cut(s) 185, 495, 1709, 1833
Ecl136II GAGCTC 1 cut(s) 488
EclXI CGGCCG 1 cut(s) 1886
Eco24I GRGCYC 2 cut(s) 490, 1723
Eco47I GGWCC 1 cut(s) 1592
Eco52I CGGCCG 1 cut(s) 1886
Eco53kI GAGCTC 1 cut(s) 488
Eco72I CACGTG 1 cut(s) 83
Eco81I CCTNAGG 1 cut(s) 1026
Eco88I CYCGRG 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 488
EcoNI CCTNNNNNAGG 1 cut(s) 1557
EcoO109I RGGNCCY 1 cut(s) 1592
EcoRI GAATTC 1 cut(s) 1328
EcoRII CCWGG 4 cut(s) 742, 898, 1552, 1558
EcoT22I ATGCAT 1 cut(s) 772
EcoT38I GRGCYC 2 cut(s) 490, 1723
FaqI GGGAC 2 cut(s) 872, 1578
FauI CCCGC 2 cut(s) 1176, 1890
FbaI TGATCA 1 cut(s) 972
FblI GTMKAC 1 cut(s) 1962
FokI GGATG 7 cut(s) 629, 726, 818, 1097, 1181, 1380, 1938
FriOI GRGCYC 2 cut(s) 490, 1723
FspBI CTAG 5 cut(s) 110, 375, 1122, 1902, 1958
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 1608
HaeIII GGCC 5 cut(s) 206, 817, 980, 1558, 1888
HapII CCGG 1 cut(s) 353
HgaI GACGC 2 cut(s) 323, 998
Hin1I GRCGYC 1 cut(s) 315
HincII GTYRAC 4 cut(s) 248, 424, 1423, 1963
HindII GTYRAC 4 cut(s) 248, 424, 1423, 1963
HindIII AAGCTT 2 cut(s) 1214, 1667
HinfI GANTC 6 cut(s) 644, 801, 936, 1381, 1545, 1652
HpaII CCGG 1 cut(s) 353
HphI GGTGA 5 cut(s) 183, 671, 857, 1283, 1469
Hpy166II GTNNAC 6 cut(s) 128, 248, 424, 598, 1423, 1963
Hpy188I TCNGA 6 cut(s) 144, 763, 1380, 1469, 1496, 1550
Hpy188III TCNNGA 6 cut(s) 471, 1198, 1220, 1587, 1625, 1724
Hpy8I GTNNAC 6 cut(s) 128, 248, 424, 598, 1423, 1963
HpyAV CCTTC 6 cut(s) 198, 228, 926, 1197, 1242, 1667
HpyCH4III ACNGT 4 cut(s) 340, 895, 1324, 1735
HpyCH4IV ACGT 3 cut(s) 82, 1000, 1785
HpyF10VI GCNNNNNNNGC 7 cut(s) 494, 1073, 1283, 1286, 1292, 1532, 1891
HpyF3I CTNAG 6 cut(s) 289, 685, 762, 819, 1026, 1493
HpySE526I ACGT 3 cut(s) 82, 1000, 1785
Hsp92I GRCGYC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 972
KspI CCGCGG 1 cut(s) 1886
Kzo9I GATC 2 cut(s) 381, 972
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 485, 1843
MaeI CTAG 5 cut(s) 110, 375, 1122, 1902, 1958
MaeII ACGT 3 cut(s) 82, 1000, 1785
MaeIII GTNAC 4 cut(s) 52, 284, 340, 1399
MalI GATC 2 cut(s) 383, 974
MbiI CCGCTC 1 cut(s) 1183
MboI GATC 2 cut(s) 381, 972
MfeI CAATTG 1 cut(s) 1506
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 8 cut(s) 130, 454, 490, 926, 1494, 1684, 1723, 1883
MmeI TCCRAC 3 cut(s) 701, 1297, 1573
Mph1103I ATGCAT 1 cut(s) 772
MroXI GAANNNNTTC 2 cut(s) 516, 1440
MseI TTAA 5 cut(s) 122, 431, 465, 1539, 1972
MslI CAYNNNNRTG 1 cut(s) 1367
MspA1I CMGCKG 3 cut(s) 1073, 1885, 1891
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 5 cut(s) 354, 744, 900, 1554, 1560
MunI CAATTG 1 cut(s) 1506
Mva1269I GAATGC 2 cut(s) 772, 1517
MvaI CCWGG 4 cut(s) 744, 900, 1554, 1560
MvnI CGCG 2 cut(s) 552, 1885
MwoI GCNNNNNNNGC 7 cut(s) 494, 1073, 1283, 1286, 1292, 1532, 1891
NciI CCSGG 1 cut(s) 354
NdeII GATC 2 cut(s) 381, 972
NlaIV GGNNCC 8 cut(s) 1208, 1321, 1351, 1594, 1638, 1683, 1749, 1880
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 2 cut(s) 340, 1399
NotI GCGGCCGC 1 cut(s) 1886
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 3 cut(s) 27, 504, 1009
NspV TTCGAA 3 cut(s) 305, 559, 1603
PaeI GCATGC 1 cut(s) 1009
PaqCI CACCTGC 1 cut(s) 872
PciI ACATGT 2 cut(s) 23, 500
PciSI GCTCTTC 1 cut(s) 495
PcsI WCGNNNNNNNCGW 2 cut(s) 556, 1006
PctI GAATGC 2 cut(s) 772, 1517
PdmI GAANNNNTTC 2 cut(s) 516, 1440
PfeI GAWTC 6 cut(s) 644, 801, 936, 1381, 1545, 1652
PflMI CCANNNNNTGG 4 cut(s) 574, 720, 749, 1875
PfoI TCCNGGA 1 cut(s) 742
PmaCI CACGTG 1 cut(s) 83
PmlI CACGTG 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 1592
PscI ACATGT 2 cut(s) 23, 500
PshAI GACNNNNGTC 1 cut(s) 24
PsiI TTATAA 1 cut(s) 1739
Psp124BI GAGCTC 1 cut(s) 490
Psp5II RGGWCCY 1 cut(s) 1592
Psp6I CCWGG 4 cut(s) 742, 898, 1552, 1558
PspCI CACGTG 1 cut(s) 83
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 4 cut(s) 742, 898, 1552, 1558
PspN4I GGNNCC 8 cut(s) 1208, 1321, 1351, 1594, 1638, 1683, 1749, 1880
PspPI GGNCC 4 cut(s) 205, 815, 1556, 1592
PspPPI RGGWCCY 1 cut(s) 1592
PstI CTGCAG 1 cut(s) 1072
PsuI RGATCY 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 4 cut(s) 683, 730, 897, 1109
RsaNI GTAC 4 cut(s) 682, 729, 896, 1108
RseI CAYNNNNRTG 1 cut(s) 1367
SacI GAGCTC 1 cut(s) 490
SacII CCGCGG 1 cut(s) 1886
SalI GTCGAC 1 cut(s) 1961
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 5 cut(s) 122, 431, 465, 1539, 1972
Sau3AI GATC 2 cut(s) 381, 972
Sau96I GGNCC 4 cut(s) 205, 815, 1556, 1592
ScrFI CCNGG 5 cut(s) 354, 744, 900, 1554, 1560
SduI GDGCHC 8 cut(s) 130, 454, 490, 926, 1494, 1684, 1723, 1883
SfcI CTRYAG 2 cut(s) 917, 1068
Sfr303I CCGCGG 1 cut(s) 1886
SfuI TTCGAA 3 cut(s) 305, 559, 1603
SgrBI CCGCGG 1 cut(s) 1886
SinI GGWCC 1 cut(s) 1592
SmiMI CAYNNNNRTG 1 cut(s) 1367
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
SphI GCATGC 1 cut(s) 1009
SsiI CCGC 7 cut(s) 194, 1183, 1258, 1853, 1883, 1885, 1889
SspI AATATT 1 cut(s) 263
SspMI CTAG 5 cut(s) 110, 375, 1122, 1902, 1958
SstI GAGCTC 1 cut(s) 490
StyD4I CCNGG 5 cut(s) 352, 742, 898, 1552, 1558
TaaI ACNGT 4 cut(s) 340, 895, 1324, 1735
TaiI ACGT 3 cut(s) 85, 1003, 1788
TaqI TCGA 4 cut(s) 305, 559, 1603, 1962
TatI WGTACW 2 cut(s) 728, 1107
TauI GCSGC 2 cut(s) 1888, 1891
TfiI GAWTC 6 cut(s) 644, 801, 936, 1381, 1545, 1652
Tru1I TTAA 5 cut(s) 122, 431, 465, 1539, 1972
Tru9I TTAA 5 cut(s) 122, 431, 465, 1539, 1972
TscAI CASTG 1 cut(s) 1738
TseFI GTSAC 2 cut(s) 340, 1399
Tsp45I GTSAC 2 cut(s) 340, 1399
TspDTI ATGAA 9 cut(s) 21, 216, 482, 614, 887, 1351, 1385, 1727, 1851
TspGWI ACGGA 3 cut(s) 148, 374, 824
TspRI CASTG 1 cut(s) 1738
Van91I CCANNNNNTGG 4 cut(s) 574, 720, 749, 1875
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 1592
XagI CCTNNNNNAGG 1 cut(s) 1557
XapI RAATTY 4 cut(s) 516, 1154, 1328, 1440
XceI RCATGY 3 cut(s) 27, 504, 1009
XcmI CCANNNNNNNNNTGG 2 cut(s) 1902, 1949
XmiI GTMKAC 1 cut(s) 1962
XmnI GAANNNNTTC 2 cut(s) 516, 1440
XspI CTAG 5 cut(s) 110, 375, 1122, 1902, 1958
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.