pycom14g01630
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008158
Physical Location & Seq
Reverse (-)
1206907 .. 1208069
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGGTCTTTCAACAGAACAACGGAAGACTAACTATAGCTGTGGAGCTGGTTACTAACCCCTCTATCATTTTCATGGATGAACCGACATCTGGTCTTGATGCCAGGGCTGCTGCTATTGCCATGCGTATTGTGAGAAACACGGTGGATACAGGCAGAACTGTTGTCTGCACAATTCACCAGCCAAGCATAGATATTTTTGAATCTTTCGACGAGTTACTCTTGATGAAACGTGGAGGGCAAGTCATTTATGCTGGACCCCTCGGCCGCCACTCTCACAAGCTTGTAGAATATTTTGAAGCCATCCCGGGTGTGCCCAAGATCGATCTTTTAATCATGACTCAACTTGATGTGGACTTTGCAGACATTTACCAAAACTCCTTGCTTTACCAGAGGAACCAGGAGCTTATCAAAGAGCTTAGTACATCGGCTCCTGGGTCCAAAGATCTCTACTTCCCAACCAAATACTCTCAACCTTTCCCGGTTCAGTGCAAAGCTTGTTTCTGGAAAATGCATTGGTCGTATTGGAGAAATCCTCAGTACAATGCCATCCGATTCTTTATGACCATTGTCATCGGTGTCTTATTCGATCTTTATCTTCTGGCAGAAGGGACAGCAGACAGCACAACAGCAAGATCTGATAAACCTTTTGGGAGCTATGTATGCTGCTGTGCTTTTCCTTGGAACCACGAATGCTTCTGTGGTCCGATTTGTTGTCGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

241

Amino Acids

27.29

Weight (kDa)

6.29

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC2_membrane PF01061 162 - 200 6.8e-06 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 265
AcoI YGGCCR 1 cut(s) 262
AfaI GTAC 2 cut(s) 421, 539
AfiI CCNNNNNNNGG 1 cut(s) 89
AgsI TTSAA 3 cut(s) 11, 200, 296
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 3 cut(s) 101, 396, 430
AluBI AGCT 7 cut(s) 38, 46, 280, 403, 415, 494, 654
AluI AGCT 7 cut(s) 38, 46, 280, 403, 415, 494, 654
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 3 cut(s) 107, 110, 663
AspS9I GGNCC 3 cut(s) 254, 435, 701
AsuC2I CCSGG 3 cut(s) 305, 306, 479
AsuHPI GGTGA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 3 cut(s) 254, 435, 701
BaeGI GKGCMC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 94, 97, 650
BccI CCATC 2 cut(s) 308, 554
BciT130I CCWGG 3 cut(s) 103, 398, 432
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 3 cut(s) 305, 306, 479
BfmI CTRYAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 139
BglII AGATCT 2 cut(s) 442, 632
BisI GCNGC 4 cut(s) 108, 111, 265, 664
BlsI GCNGC 4 cut(s) 109, 112, 266, 665
Bme1390I CCNGG 6 cut(s) 103, 305, 306, 398, 432, 479
Bme18I GGWCC 3 cut(s) 254, 435, 701
BmeRI GACNNNNNGTC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 304
BmgT120I GGNCC 3 cut(s) 254, 435, 701
BmiI GGNNCC 5 cut(s) 256, 395, 429, 436, 683
BmrFI CCNGG 6 cut(s) 103, 305, 306, 398, 432, 479
BmsI GCATC 1 cut(s) 88
BoxI GACNNNNGTC 1 cut(s) 566
BpiI GAAGAC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 517, 549
BpuMI CCSGG 3 cut(s) 305, 306, 479
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 591
BsaJI CCNNGG 5 cut(s) 102, 259, 304, 431, 677
BsaXI ACNNNNNCTCC 6 cut(s) 225, 255, 359, 389, 412, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 3 cut(s) 103, 398, 432
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 5 cut(s) 102, 259, 304, 431, 677
BseGI GGATG 3 cut(s) 82, 300, 546
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMII CTCAG 1 cut(s) 548
BseSI GKGCMC 1 cut(s) 315
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 3 cut(s) 94, 97, 650
BsgI GTGCAG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 2 cut(s) 305, 479
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 622
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 5 cut(s) 318, 322, 442, 586, 632
BspACI CCGC 1 cut(s) 265
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 547
BspDI ATCGAT 1 cut(s) 321
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 5 cut(s) 256, 395, 429, 436, 683
BssECI CCNNGG 5 cut(s) 102, 259, 304, 431, 677
BssMI GATC 5 cut(s) 318, 322, 442, 586, 632
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 3 cut(s) 103, 398, 432
Bst4CI ACNGT 2 cut(s) 142, 160
BstDEI CTNAG 2 cut(s) 416, 534
BstF5I GGATG 3 cut(s) 82, 300, 546
BstKTI GATC 5 cut(s) 321, 325, 445, 589, 635
BstMBI GATC 5 cut(s) 318, 322, 442, 586, 632
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 107, 116, 660
BstNI CCWGG 3 cut(s) 103, 398, 432
BstPAI GACNNNNGTC 1 cut(s) 566
BstSCI CCNGG 6 cut(s) 101, 303, 304, 396, 430, 477
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 94, 97, 650
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 442, 632
BstYI RGATCY 2 cut(s) 442, 632
BstZI CGGCCG 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 3 cut(s) 82, 300, 546
BtsIMutI CAGTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 333
Cfr13I GGNCC 3 cut(s) 254, 435, 701
Cfr9I CCCGGG 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 2 cut(s) 420, 538
CviAII CATG 3 cut(s) 73, 121, 334
CviQI GTAC 2 cut(s) 420, 538
DdeI CTNAG 2 cut(s) 416, 534
DpnI GATC 5 cut(s) 320, 324, 444, 588, 634
DpnII GATC 5 cut(s) 318, 322, 442, 586, 632
DriI GACNNNNNGTC 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
Eam1105I GACNNNNNGTC 1 cut(s) 90
EclXI CGGCCG 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 677
Eco47I GGWCC 3 cut(s) 254, 435, 701
Eco52I CGGCCG 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 304
EcoRII CCWGG 3 cut(s) 101, 396, 430
EcoT14I CCWWGG 1 cut(s) 677
EcoT22I ATGCAT 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 3 cut(s) 76, 124, 337
FaiI YATR 9 cut(s) 35, 74, 122, 188, 249, 335, 560, 657, 661
FaqI GGGAC 1 cut(s) 622
FatI CATG 3 cut(s) 72, 120, 333
Fnu4HI GCNGC 4 cut(s) 108, 111, 265, 664
FokI GGATG 3 cut(s) 89, 287, 533
Fsp4HI GCNGC 4 cut(s) 108, 111, 265, 664
GluI GCNGC 4 cut(s) 108, 111, 265, 664
HaeIII GGCC 1 cut(s) 264
HapII CCGG 2 cut(s) 305, 479
Hin1II CATG 3 cut(s) 76, 124, 337
HindIII AAGCTT 2 cut(s) 278, 492
HinfI GANTC 3 cut(s) 200, 337, 552
HpaII CCGG 2 cut(s) 305, 479
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 551, 637, 705
Hpy188III TCNNGA 4 cut(s) 95, 220, 334, 502
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 599
HpyCH4III ACNGT 2 cut(s) 142, 160
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 4 cut(s) 168, 359, 489, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 116, 660
HpyF3I CTNAG 2 cut(s) 416, 534
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 3 cut(s) 76, 124, 337
Kzo9I GATC 5 cut(s) 318, 322, 442, 586, 632
LmnI GCTCC 4 cut(s) 43, 400, 433, 651
Lsp1109I GCAGC 3 cut(s) 94, 97, 650
LweI GCATC 1 cut(s) 88
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 2 cut(s) 49, 213
MalI GATC 5 cut(s) 320, 324, 444, 588, 634
MboI GATC 5 cut(s) 318, 322, 442, 586, 632
MboII GAAGA 2 cut(s) 36, 587
MflI RGATCY 2 cut(s) 442, 632
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 1 cut(s) 171
MlyI GAGTC 1 cut(s) 331
MnlI CCTC 5 cut(s) 70, 227, 269, 384, 543
Mph1103I ATGCAT 1 cut(s) 513
MseI TTAA 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 2 cut(s) 305, 479
MspR9I CCNGG 6 cut(s) 103, 305, 306, 398, 432, 479
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 3 cut(s) 103, 398, 432
MwoI GCNNNNNNNGC 3 cut(s) 107, 116, 660
NciI CCSGG 3 cut(s) 305, 306, 479
NdeII GATC 5 cut(s) 318, 322, 442, 586, 632
NlaIII CATG 3 cut(s) 76, 124, 337
NlaIV GGNNCC 5 cut(s) 256, 395, 429, 436, 683
NmeAIII GCCGAG 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 513
PagI TCATGA 1 cut(s) 333
PctI GAATGC 1 cut(s) 695
PfeI GAWTC 2 cut(s) 200, 552
PkrI GCNGC 4 cut(s) 109, 112, 266, 665
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 101, 396, 430
PspGI CCWGG 3 cut(s) 101, 396, 430
PspN4I GGNNCC 5 cut(s) 256, 395, 429, 436, 683
PspPI GGNCC 3 cut(s) 254, 435, 701
PsuI RGATCY 2 cut(s) 442, 632
RsaI GTAC 2 cut(s) 421, 539
RsaNI GTAC 2 cut(s) 420, 538
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 4 cut(s) 108, 111, 265, 664
Sau3AI GATC 5 cut(s) 318, 322, 442, 586, 632
Sau96I GGNCC 3 cut(s) 254, 435, 701
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 6 cut(s) 103, 305, 306, 398, 432, 479
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 3 cut(s) 254, 435, 701
SmaI CCCGGG 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 171
SsiI CCGC 1 cut(s) 265
SspI AATATT 1 cut(s) 290
StyD4I CCNGG 6 cut(s) 101, 303, 304, 396, 430, 477
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 2 cut(s) 142, 160
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 207, 321, 585
TasI AATT 1 cut(s) 171
TatI WGTACW 2 cut(s) 419, 537
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 2 cut(s) 200, 552
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 1 cut(s) 491
TseI GCWGC 3 cut(s) 107, 110, 663
TspDTI ATGAA 3 cut(s) 61, 93, 239
TspGWI ACGGA 1 cut(s) 36
TspMI CCCGGG 1 cut(s) 304
TspRI CASTG 1 cut(s) 491
VpaK11BI GGWCC 3 cut(s) 254, 435, 701
XmaI CCCGGG 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.