pycom14g03140

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004746
Physical Location & Seq
Reverse (-)
2435925 .. 2436476
552 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGCCTACAACAACCAGAAGAAAGCTCCGGCTGAGCAAAGTCACAGTCGACATAGGATGCGGCAGCTGCAGAAAACCTAGTAAGCTCTTCCAAATATTCAACCCTAAACCTAAACCCTCCTCCAAAACCCTAAAACACAACCAATTTCATCATCCTGCTTCTTCATCTTCAACCCCACGCAACAATTACAATAAACTCTACAAAGACAACTACCACGCTCCTTTTTTTGTCACAGACTCCGACACGAAGAGCACGCGACCAATCAACGGCTTCGGTAGAATCGGCAATGAGAGCGTGGCCGTGGAGAAAGACTCCGGCGACCCTTACTTGGATTTTCGACACTCCATGCTACAGATGATACTGGAGAACGAGATATACTCCAAAGACGATCGATCCGGGAGCTTCTCAACTGTTTCCTGCAGCTCAACTCTCCCTGCAACCACGGCATTATCATCAGGGTCTTCACTGAGATCTGGAATGCTGTTTTCTCTGTCAGGTCTGGAGGTTCGACGTTGCTGCACTTTTGCGAGCAACAAGTCATGTGACTACTAG

Protein Analysis

184

Amino Acids

20.34

Weight (kDa)

9.55

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 105 - 130 2.4e-06 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccII CGCG 1 cut(s) 256
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 266, 328
AgsI TTSAA 2 cut(s) 100, 171
AluBI AGCT 5 cut(s) 25, 66, 85, 402, 423
AluI AGCT 5 cut(s) 25, 66, 85, 402, 423
Alw21I GWGCWC 1 cut(s) 254
AlwI GGATC 1 cut(s) 387
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 4 cut(s) 63, 66, 420, 516
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsuC2I CCSGG 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 4 cut(s) 53, 75, 432, 503
BceAI ACGGC 3 cut(s) 283, 284, 459
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BcnI CCSGG 1 cut(s) 397
BfaI CTAG 2 cut(s) 78, 550
BfmI CTRYAG 3 cut(s) 67, 350, 418
BglII AGATCT 1 cut(s) 470
BisI GCNGC 5 cut(s) 61, 64, 67, 421, 517
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 5 cut(s) 62, 65, 68, 422, 518
Bme1390I CCNGG 1 cut(s) 397
BmrFI CCNGG 1 cut(s) 397
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 453
BplI GAGNNNNNCTC 4 cut(s) 296, 328, 362, 394
BpmI CTGGAG 2 cut(s) 383, 521
Bpu1102I GCTNAGC 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 397
Bsa29I ATCGAT 1 cut(s) 391
BsaJI CCNNGG 2 cut(s) 300, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 328
Bse1I ACTGG 1 cut(s) 366
Bse3DI GCAATG 1 cut(s) 292
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 300, 441
BseGI GGATG 2 cut(s) 62, 151
BseLI CCNNNNNNNGG 2 cut(s) 266, 328
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 23, 458
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 4 cut(s) 53, 75, 432, 503
BsgI GTGCAG 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 28, 315, 396
BslI CCNNNNNNNGG 2 cut(s) 266, 328
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 3 cut(s) 388, 392, 470
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 24, 459
BspDI ATCGAT 1 cut(s) 391
BspFNI CGCG 1 cut(s) 256
BspMAI CTGCAG 2 cut(s) 71, 422
BspPI GGATC 1 cut(s) 387
BspQI GCTCTTC 2 cut(s) 92, 242
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 2 cut(s) 300, 441
BssMI GATC 3 cut(s) 388, 392, 470
Bst4CI ACNGT 2 cut(s) 46, 412
Bst6I CTCTTC 2 cut(s) 92, 242
BstC8I GCNNGC 2 cut(s) 254, 529
BstDEI CTNAG 2 cut(s) 32, 467
BstDSI CCRYGG 2 cut(s) 300, 441
BstF5I GGATG 2 cut(s) 62, 151
BstFNI CGCG 1 cut(s) 256
BstKTI GATC 3 cut(s) 391, 395, 473
BstMBI GATC 3 cut(s) 388, 392, 470
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 66, 291, 443
BstSCI CCNGG 1 cut(s) 395
BstSFI CTRYAG 3 cut(s) 67, 350, 418
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 4 cut(s) 53, 75, 432, 503
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
Bsu15I ATCGAT 1 cut(s) 391
BsuRI GGCC 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 391
BtgI CCRYGG 2 cut(s) 300, 441
BtsCI GGATG 2 cut(s) 62, 151
BtsIMutI CAGTG 1 cut(s) 464
Cac8I GCNNGC 2 cut(s) 254, 529
ClaI ATCGAT 1 cut(s) 391
CviAII CATG 2 cut(s) 346, 540
CviJI RGCY 8 cut(s) 25, 31, 66, 85, 270, 299, 402, 423
CviKI_1 RGCY 8 cut(s) 25, 31, 66, 85, 270, 299, 402, 423
DdeI CTNAG 2 cut(s) 32, 467
DpnI GATC 3 cut(s) 390, 394, 472
DpnII GATC 3 cut(s) 388, 392, 470
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 92, 242
EarI CTCTTC 2 cut(s) 92, 242
FaeI CATG 2 cut(s) 349, 543
FaiI YATR 4 cut(s) 53, 347, 376, 541
FatI CATG 2 cut(s) 345, 539
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 5 cut(s) 61, 64, 67, 421, 517
FokI GGATG 2 cut(s) 69, 138
Fsp4HI GCNGC 5 cut(s) 61, 64, 67, 421, 517
FspBI CTAG 2 cut(s) 78, 550
GluI GCNGC 5 cut(s) 61, 64, 67, 421, 517
GsuI CTGGAG 2 cut(s) 383, 521
HaeIII GGCC 1 cut(s) 299
HapII CCGG 3 cut(s) 28, 315, 396
Hin1II CATG 2 cut(s) 349, 543
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 3 cut(s) 236, 279, 311
HpaII CCGG 3 cut(s) 28, 315, 396
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 2 cut(s) 474, 500
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 1 cut(s) 513
HpyCH4III ACNGT 2 cut(s) 46, 412
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 4 cut(s) 69, 420, 437, 519
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 291, 443
HpyF3I CTNAG 2 cut(s) 32, 467
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 2 cut(s) 349, 543
Kzo9I GATC 3 cut(s) 388, 392, 470
LguI GCTCTTC 2 cut(s) 92, 242
LmnI GCTCC 3 cut(s) 30, 223, 399
Lsp1109I GCAGC 4 cut(s) 53, 75, 432, 503
LweI GCATC 1 cut(s) 47
MaeI CTAG 2 cut(s) 78, 550
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 3 cut(s) 40, 229, 542
MalI GATC 3 cut(s) 390, 394, 472
MboI GATC 3 cut(s) 388, 392, 470
MboII GAAGA 6 cut(s) 30, 79, 153, 159, 259, 453
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 2 cut(s) 143, 184
MlyI GAGTC 2 cut(s) 230, 305
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 3 cut(s) 127, 130, 496
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 28, 315, 396
MspR9I CCNGG 1 cut(s) 397
Mva1269I GAATGC 1 cut(s) 483
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 3 cut(s) 66, 291, 443
NciI CCSGG 1 cut(s) 397
NdeII GATC 3 cut(s) 388, 392, 470
NlaIII CATG 2 cut(s) 349, 543
NmuCI GTSAC 3 cut(s) 40, 229, 542
PciSI GCTCTTC 2 cut(s) 92, 242
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 279
PfoI TCCNGGA 1 cut(s) 395
PkrI GCNGC 5 cut(s) 62, 65, 68, 422, 518
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 2 cut(s) 230, 305
PpsI GAGTC 2 cut(s) 230, 305
PsrI GAACNNNNNNTAC 2 cut(s) 359, 391
PstI CTGCAG 2 cut(s) 71, 422
PsuI RGATCY 1 cut(s) 470
PvuI CGATCG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 66
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 92, 242
SatI GCNGC 5 cut(s) 61, 64, 67, 421, 517
Sau3AI GATC 3 cut(s) 388, 392, 470
SchI GAGTC 2 cut(s) 230, 305
ScrFI CCNGG 1 cut(s) 397
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 1 cut(s) 47
SfcI CTRYAG 3 cut(s) 67, 350, 418
Sse9I AATT 2 cut(s) 143, 184
SsiI CCGC 1 cut(s) 60
SspI AATATT 1 cut(s) 96
SspMI CTAG 2 cut(s) 78, 550
StyD4I CCNGG 1 cut(s) 395
TaaI ACNGT 2 cut(s) 46, 412
TaiI ACGT 1 cut(s) 514
TaqI TCGA 4 cut(s) 48, 337, 391, 508
TasI AATT 2 cut(s) 143, 184
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 1 cut(s) 279
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 3 cut(s) 40, 229, 542
TseI GCWGC 4 cut(s) 63, 66, 420, 516
Tsp45I GTSAC 3 cut(s) 40, 229, 542
TspDTI ATGAA 2 cut(s) 137, 153
TspRI CASTG 1 cut(s) 471
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 78, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.