pycom14g05590

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003053
Physical Location & Seq
Forward (+)
5390452 .. 5397944
7493 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1941 bp
ATGGAGGGAGGGAAGACGAAGCAGAAATTGCCGGCTCTCACTCTCCAACAGTTCATCTCCACCACCGCCCCTCTTATCGACCTCGAAAAGGAAGCTGAGATATCTGCCTCCATCATAACTGGGTCTTCCAGAAACTTGGACACTGCTCAGAAGAAGGGCTCCACCATTCTCAATTTGAAATGTGTTGATGCTCAGACAGGACTCATGGGGAAGAGTCTTCTTGAGTTTCAATCCACCAAAGCAGATGTTCTTCCTCCCCACAAGTTTAGCACCCATGATGTGGTTGTTCTAAAACAAAATAAGGCTGATCTGGGGGCCCCTGCTCTTGGACAAGGTGTAGTTTATCGGTTAAAGGACTCATCAATCATTGTTGCTTTTGATGATGTTCCTGAAGATGGTTTAAATAGTCCTCTACGGTTGGAGAAACTGGGAAATGAGGTGACATATCAGAGGATGAAGGATGCCTTGATACAATTGAGCAAAGGTGTGCAGAAGGGCCCCGCATCTGATTTAATTCCTGTCTTATTTGGAGAGAGGACACCAACAGTGTCCAAGAAGGATGTCACATTCACCCCATTTAACAAAAACCTTGATCATTCTCAGAGAGATGCCATTTCAAAGGCCCTGTCATCAAAGAATGTATTTTTGCTGCATGGACCTCCTGGCACGGGAAAGACTACAACTGTGGTGGAAATTATCTTGCAAGAAGTTAAACGTGGATCAAAGATTCTTGCTTGTGCTGCTTCAAATATTGCTGTTGACAACATTGTTGAGCGACTTGCACGCCACAAAGTAAAGTTAGTGAGAGTGGGGCATCCTGCACGTTTACTACCTCAAGTGCTGGACAGTGCACTGGATGCACAGGTCTTGCGAGGGGATAACAGTTCTCTGGCAAATGACATTCGGAAGGAAATGAAGGCATTAAATGGGAAGCTCTTAAGGACCAAAGATAAGAACACTAGGAGGGAAATCCAGAAGGAGCTTAGGACTCTTTCTAGGGAAGAGCGTAAAAGGCAGCAGCTAGCTGTAACAGATGTAATAAAAAATGCAGATGTGATTTTGACAACTTTGACTGGGGCATCTTCTCGCAAGCTCGACAATACTTCATTTGATTTGGTGATTATTGATGAAGCTGCTCAGGCACTTGAGATAGCATGCTGGATGGCTTTACTAAAGGGTTCAAGATGTATACTTGCAGGCGACCATCTTCAGCTTCCTCCTACCATTCAGAGTGCTGAAGCTGAGAAGAAAGGTCTAGGAAGGACCCTCTTTGAACGCCTTGCAGACATATATGGAAATGAAGTCATGTCTATGCTCACTGTCCAGTACCGCATGCACGATCGATCATGGATTGGTCATCAAAGGAGCTTTACAACAGTAAGTAAGGCCCATTCAAGTGTTGCTGGGCATATGCTTTTGGACCTGGAGGATGTAAAGACGACCTCTTCTACAGAACCGACCCTTCTTCTAATAGACACAGCTGGGTGTGACATGGAAGAAAAGAAGGATGAAGAAGAAAGCACTTTGAATGAGGGGGAAGCCGATGTTGCTATTGCCCATGCCAAGAGACTTGTTCAAAGCGGTGTTCAGGCTTCTGATATTGGAATTATCACTCCTTATGCTGCACAGGTTGTCGTACTCAGGATGTTGAGAAGCAATGACGACAAGCTAAAGGATTTGGAAATCTCAACAGTTGACGGCTTCCAGGGCCGGGAAAAGGAAGCCATCATTATTTCAATGGTTCGGTCAAATTCAAAAAAAGAGGTAGGGTTTCTGAAGGACCACAGGCGAATGAACGTGGCTGTAACACGTGCAAGAAGGCAATGCTGTCTTGTATGCGACACAGACACGGTGACTAGTGATGCATTCTTGAAGCGATTGATAGAGTATTTTGAGGAGCATGGTGAGTATCTAAGTGCCTCAGAGTATAGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000049 GO:0000122 GO:0000166 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003008 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003690 GO:0003697 GO:0003700 GO:0003723 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007417 GO:0008026 GO:0008094 GO:0008134 GO:0008144 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0021510 GO:0021515 GO:0021517 GO:0021522 GO:0021953 GO:0022008 GO:0022607 GO:0030154 GO:0030182 GO:0030424 GO:0030426 GO:0030427 GO:0030554 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0032553 GO:0032555 GO:0032559 GO:0032574 GO:0032575 GO:0032991 GO:0033267 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0036477 GO:0042623 GO:0042995 GO:0043005 GO:0043021 GO:0043022 GO:0043025 GO:0043043 GO:0043139 GO:0043141 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043603 GO:0043604 GO:0043621 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0044877 GO:0045892 GO:0045934 GO:0048471 GO:0048513 GO:0048519 GO:0048523 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050877 GO:0050905 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051259 GO:0051260 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097367 GO:0097458 GO:0120025 GO:0120038 GO:0140097 GO:0140098 GO:0140110 GO:0150034 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:1990904 GO:1990955 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

647

Amino Acids

71.2

Weight (kDa)

8.23

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMUBP-2_HCS1_1B PF21138 21 - 130 7.6e-18 Helicase SMUBP-2/HCS1, 1B domain
AAA_11 PF13086 196 - 411 8.6e-67 AAA domain
AAA_30 PF13604 197 - 261 5.1e-08 AAA domain
AAA_19 PF13245 201 - 408 5.1e-11 AAA domain
AAA_12 PF13087 420 - 615 1.4e-51 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 280
AccI GTMKAC 1 cut(s) 1189
AciI CCGC 4 cut(s) 66, 501, 1330, 1581
AclWI GGATC 1 cut(s) 727
AcsI RAATTY 1 cut(s) 1750
AcuI CTGAAG 4 cut(s) 411, 1193, 1257, 1796
AcvI CACGTG 1 cut(s) 1811
AfaI GTAC 2 cut(s) 1328, 1638
AfiI CCNNNNNNNGG 7 cut(s) 88, 280, 326, 395, 668, 1711, 1717
AflII CTTAAG 1 cut(s) 937
AflIII ACRYGT 1 cut(s) 1808
AhlI ACTAGT 1 cut(s) 1856
AjnI CCWGG 3 cut(s) 661, 1422, 1704
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 1 cut(s) 1561
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 1 cut(s) 727
AoxI GGCC 5 cut(s) 315, 496, 621, 1386, 1708
ApaI GGGCCC 2 cut(s) 319, 500
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1622
ApoI RAATTY 1 cut(s) 1750
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
AsuC2I CCSGG 1 cut(s) 1712
AsuHPI GGTGA 5 cut(s) 451, 562, 1129, 1864, 1916
AsuNHI GCTAGC 1 cut(s) 1021
AvaII GGWCC 5 cut(s) 656, 942, 1263, 1420, 1780
BaeGI GKGCMC 3 cut(s) 319, 500, 853
BanII GRGCYC 3 cut(s) 161, 319, 500
BbrPI CACGTG 1 cut(s) 1811
BbsI GAAGAC 3 cut(s) 20, 117, 209
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1609
BccI CCATC 5 cut(s) 119, 389, 1156, 1212, 1733
BceAI ACGGC 1 cut(s) 1714
BciT130I CCWGG 3 cut(s) 663, 1424, 1706
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 1712
BcoDI GTCTC 1 cut(s) 1561
BcuI ACTAGT 1 cut(s) 1856
BfaI CTAG 5 cut(s) 960, 996, 1022, 1256, 1857
BfmI CTRYAG 1 cut(s) 1449
BfrI CTTAAG 1 cut(s) 937
BisI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1623
BlsI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1624
Bme1390I CCNGG 4 cut(s) 663, 1424, 1706, 1712
Bme18I GGWCC 5 cut(s) 656, 942, 1263, 1420, 1780
BmiI GGNNCC 7 cut(s) 160, 316, 317, 318, 498, 499, 1265
BmrFI CCNGG 4 cut(s) 663, 1424, 1706, 1712
BmrI ACTGGG 3 cut(s) 129, 437, 1083
BmsI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1852
BmtI GCTAGC 1 cut(s) 1025
BmuI ACTGGG 3 cut(s) 129, 437, 1083
BpiI GAAGAC 3 cut(s) 20, 117, 209
BpmI CTGGAG 1 cut(s) 1445
Bpu10I CCTNAGC 2 cut(s) 983, 1137
BpuEI CTTGAG 3 cut(s) 242, 819, 1166
BpuMI CCSGG 1 cut(s) 1712
Bsa29I ATCGAT 1 cut(s) 1342
BsaAI YACGTR 1 cut(s) 1811
BsaJI CCNNGG 1 cut(s) 1705
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 280, 326, 395, 668, 1711, 1717
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 5 cut(s) 124, 432, 858, 1078, 1324
Bse3DI GCAATG 3 cut(s) 1663, 1829, 1939
BseBI CCWGG 3 cut(s) 663, 1424, 1706
BseCI ATCGAT 1 cut(s) 1342
BseDI CCNNGG 1 cut(s) 1705
BseGI GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1435, 1513, 1650
BseLI CCNNNNNNNGG 7 cut(s) 88, 280, 326, 395, 668, 1711, 1717
BseMI GCAATG 3 cut(s) 1663, 1829, 1939
BseMII CTCAG 8 cut(s) 87, 161, 206, 614, 1151, 1233, 1654, 1935
BseNI ACTGG 5 cut(s) 124, 432, 858, 1078, 1324
BseRI GAGGAG 1 cut(s) 1910
BseSI GKGCMC 3 cut(s) 319, 500, 853
BseXI GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1609
BseYI CCCAGC 2 cut(s) 1403, 1481
BsgI GTGCAG 3 cut(s) 509, 804, 1608
Bsh1285I CGRYCG 1 cut(s) 1342
BshFI GGCC 5 cut(s) 317, 498, 623, 1388, 1710
BshVI ATCGAT 1 cut(s) 1342
BsiEI CGRYCG 1 cut(s) 1342
BsiHKAI GWGCWC 1 cut(s) 853
BsiSI CCGG 2 cut(s) 32, 1711
BslI CCNNNNNNNGG 7 cut(s) 88, 280, 326, 395, 668, 1711, 1717
BsmAI GTCTC 1 cut(s) 1561
BsmI GAATGC 1 cut(s) 1865
BsnI GGCC 5 cut(s) 317, 498, 623, 1388, 1710
Bsp120I GGGCCC 2 cut(s) 315, 496
Bsp1286I GDGCHC 4 cut(s) 161, 319, 500, 853
Bsp143I GATC 5 cut(s) 307, 592, 719, 1339, 1343
BspACI CCGC 4 cut(s) 66, 501, 1330, 1581
BspANI GGCC 5 cut(s) 317, 498, 623, 1388, 1710
BspCNI CTCAG 8 cut(s) 88, 160, 205, 613, 1150, 1234, 1653, 1934
BspDI ATCGAT 1 cut(s) 1342
BspLI GGNNCC 7 cut(s) 160, 316, 317, 318, 498, 499, 1265
BspOI GCTAGC 1 cut(s) 1025
BspPI GGATC 1 cut(s) 727
BspQI GCTCTTC 1 cut(s) 996
BspTI CTTAAG 1 cut(s) 937
BsrDI GCAATG 3 cut(s) 1663, 1829, 1939
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 5 cut(s) 124, 432, 858, 1078, 1324
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 1705
BssMI GATC 5 cut(s) 307, 592, 719, 1339, 1343
BssNAI GTATAC 1 cut(s) 1190
Bst1107I GTATAC 1 cut(s) 1190
Bst2UI CCWGG 3 cut(s) 663, 1424, 1706
Bst6I CTCTTC 3 cut(s) 206, 996, 1450
BstAFI CTTAAG 1 cut(s) 937
BstAPI GCANNNNNTGC 2 cut(s) 28, 857
BstBAI YACGTR 1 cut(s) 1811
BstC8I GCNNGC 7 cut(s) 33, 784, 1023, 1091, 1156, 1198, 1334
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1435, 1513, 1650
BstKTI GATC 5 cut(s) 310, 595, 722, 1342, 1346
BstMAI GTCTC 1 cut(s) 1561
BstMBI GATC 5 cut(s) 307, 592, 719, 1339, 1343
BstMCI CGRYCG 1 cut(s) 1342
BstMWI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1547, 1707
BstNI CCWGG 3 cut(s) 663, 1424, 1706
BstNSI RCATGY 2 cut(s) 1158, 1336
BstSCI CCNGG 4 cut(s) 661, 1422, 1704, 1710
BstSFI CTRYAG 1 cut(s) 1449
BstSLI GKGCMC 3 cut(s) 319, 500, 853
BstV1I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1609
BstV2I GAAGAC 3 cut(s) 20, 117, 209
BstXI CCANNNNNNTGG 1 cut(s) 136
BstZ17I GTATAC 1 cut(s) 1190
Bsu15I ATCGAT 1 cut(s) 1342
BsuRI GGCC 5 cut(s) 317, 498, 623, 1388, 1710
BsuTUI ATCGAT 1 cut(s) 1342
BtsCI GGATG 9 cut(s) 459, 466, 565, 814, 862, 1167, 1435, 1513, 1650
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 5 cut(s) 141, 552, 851, 853, 1317
Cac8I GCNNGC 7 cut(s) 33, 784, 1023, 1091, 1156, 1198, 1334
Cfr10I RCCGGY 1 cut(s) 31
ClaI ATCGAT 1 cut(s) 1342
Csp6I GTAC 2 cut(s) 1327, 1637
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviQI GTAC 2 cut(s) 1327, 1637
DpnI GATC 5 cut(s) 309, 594, 721, 1341, 1345
DpnII GATC 5 cut(s) 307, 592, 719, 1339, 1343
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 206, 996, 1450
EarI CTCTTC 3 cut(s) 206, 996, 1450
Eco24I GRGCYC 3 cut(s) 161, 319, 500
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 5 cut(s) 656, 942, 1263, 1420, 1780
Eco57I CTGAAG 4 cut(s) 411, 1193, 1257, 1796
Eco72I CACGTG 1 cut(s) 1811
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 6 cut(s) 315, 316, 496, 497, 622, 1263
EcoRII CCWGG 3 cut(s) 661, 1422, 1704
EcoRV GATATC 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 1867
EcoT38I GRGCYC 3 cut(s) 161, 319, 500
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 1 cut(s) 1410
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 1189
Fnu4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1623
FokI GGATG 9 cut(s) 466, 473, 572, 801, 869, 1174, 1442, 1520, 1657
FriOI GRGCYC 3 cut(s) 161, 319, 500
Fsp4HI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1623
FspBI CTAG 5 cut(s) 960, 996, 1022, 1256, 1857
GluI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1623
GsaI CCCAGC 2 cut(s) 1407, 1485
GsuI CTGGAG 1 cut(s) 1445
HaeIII GGCC 5 cut(s) 317, 498, 623, 1388, 1710
HapII CCGG 2 cut(s) 32, 1711
HincII GTYRAC 2 cut(s) 760, 1696
HindII GTYRAC 2 cut(s) 760, 1696
HinfI GANTC 5 cut(s) 201, 214, 356, 727, 988
HpaII CCGG 2 cut(s) 32, 1711
HphI GGTGA 5 cut(s) 451, 562, 1129, 1864, 1916
Hpy166II GTNNAC 5 cut(s) 760, 827, 851, 1190, 1696
Hpy188III TCNNGA 7 cut(s) 129, 221, 389, 973, 1182, 1642, 1870
Hpy8I GTNNAC 5 cut(s) 760, 827, 851, 1190, 1696
HpyCH4IV ACGT 4 cut(s) 715, 823, 1797, 1810
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1547, 1707
HpySE526I ACGT 4 cut(s) 715, 823, 1797, 1810
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 5 cut(s) 307, 592, 719, 1339, 1343
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 164, 979, 1365, 1897
Lsp1109I GCAGC 6 cut(s) 636, 727, 1027, 1030, 1120, 1609
LweI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1852
MaeI CTAG 5 cut(s) 960, 996, 1022, 1256, 1857
MaeII ACGT 4 cut(s) 715, 823, 1797, 1810
MaeIII GTNAC 6 cut(s) 439, 562, 1027, 1487, 1804, 1852
MalI GATC 5 cut(s) 309, 594, 721, 1341, 1345
MboI GATC 5 cut(s) 307, 592, 719, 1339, 1343
MfeI CAATTG 1 cut(s) 473
MhlI GDGCHC 4 cut(s) 161, 319, 500, 853
MluCI AATT 7 cut(s) 26, 172, 473, 513, 693, 1605, 1750
MlyI GAGTC 4 cut(s) 195, 223, 350, 982
MmeI TCCRAC 2 cut(s) 70, 399
Mph1103I ATGCAT 1 cut(s) 1867
MroNI GCCGGC 1 cut(s) 31
MseI TTAA 7 cut(s) 350, 401, 512, 579, 711, 923, 938
MslI CAYNNNNRTG 2 cut(s) 1310, 1395
MspA1I CMGCKG 1 cut(s) 1481
MspCI CTTAAG 1 cut(s) 937
MspI CCGG 2 cut(s) 32, 1711
MspR9I CCNGG 4 cut(s) 663, 1424, 1706, 1712
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 1865
MvaI CCWGG 3 cut(s) 663, 1424, 1706
MwoI GCNNNNNNNGC 7 cut(s) 28, 740, 857, 1012, 1139, 1547, 1707
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 1712
NdeI CATATG 1 cut(s) 1410
NdeII GATC 5 cut(s) 307, 592, 719, 1339, 1343
NgoMIV GCCGGC 1 cut(s) 31
NheI GCTAGC 1 cut(s) 1021
NlaIV GGNNCC 7 cut(s) 160, 316, 317, 318, 498, 499, 1265
NmuCI GTSAC 4 cut(s) 439, 562, 1487, 1852
NsiI ATGCAT 1 cut(s) 1867
NspI RCATGY 2 cut(s) 1158, 1336
PaeI GCATGC 2 cut(s) 1158, 1336
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 1 cut(s) 1865
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 727
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 6 cut(s) 651, 742, 1017, 1020, 1135, 1624
Ple19I CGATCG 1 cut(s) 1342
PleI GAGTC 4 cut(s) 195, 222, 350, 982
PmaCI CACGTG 1 cut(s) 1811
PmlI CACGTG 1 cut(s) 1811
PpsI GAGTC 4 cut(s) 195, 222, 350, 982
Ppu21I YACGTR 1 cut(s) 1811
PpuMI RGGWCCY 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 1263
Psp6I CCWGG 3 cut(s) 661, 1422, 1704
PspCI CACGTG 1 cut(s) 1811
PspFI CCCAGC 2 cut(s) 1403, 1481
PspGI CCWGG 3 cut(s) 661, 1422, 1704
PspN4I GGNNCC 7 cut(s) 160, 316, 317, 318, 498, 499, 1265
PspOMI GGGCCC 2 cut(s) 315, 496
PspPPI RGGWCCY 1 cut(s) 1263
PvuI CGATCG 1 cut(s) 1342
PvuII CAGCTG 1 cut(s) 1481
RsaI GTAC 2 cut(s) 1328, 1638
RsaNI GTAC 2 cut(s) 1327, 1637
RseI CAYNNNNRTG 2 cut(s) 1310, 1395
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 7 cut(s) 350, 401, 512, 579, 711, 923, 938
SatI GCNGC 6 cut(s) 650, 741, 1016, 1019, 1134, 1623
Sau3AI GATC 5 cut(s) 307, 592, 719, 1339, 1343
SchI GAGTC 4 cut(s) 195, 223, 350, 982
ScrFI CCNGG 4 cut(s) 663, 1424, 1706, 1712
SduI GDGCHC 4 cut(s) 161, 319, 500, 853
SfaNI GCATC 8 cut(s) 178, 451, 512, 598, 823, 847, 1088, 1852
SfcI CTRYAG 1 cut(s) 1449
SinI GGWCC 5 cut(s) 656, 942, 1263, 1420, 1780
SmiMI CAYNNNNRTG 2 cut(s) 1310, 1395
SmlI CTYRAG 4 cut(s) 221, 834, 937, 1145
SmoI CTYRAG 4 cut(s) 221, 834, 937, 1145
SpeI ACTAGT 1 cut(s) 1856
SphI GCATGC 2 cut(s) 1158, 1336
Sse9I AATT 7 cut(s) 26, 172, 473, 513, 693, 1605, 1750
SsiI CCGC 4 cut(s) 66, 501, 1330, 1581
SspI AATATT 1 cut(s) 751
SspMI CTAG 5 cut(s) 960, 996, 1022, 1256, 1857
StyD4I CCNGG 4 cut(s) 661, 1422, 1704, 1710
TaiI ACGT 4 cut(s) 718, 826, 1800, 1813
TaqI TCGA 4 cut(s) 78, 84, 1095, 1342
TaqII GACCGA 1 cut(s) 1734
TasI AATT 7 cut(s) 26, 172, 473, 513, 693, 1605, 1750
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 7 cut(s) 350, 401, 512, 579, 711, 923, 938
Tru9I TTAA 7 cut(s) 350, 401, 512, 579, 711, 923, 938
TscAI CASTG 5 cut(s) 148, 552, 853, 858, 1324
TseFI GTSAC 4 cut(s) 439, 562, 1487, 1852
TseI GCWGC 6 cut(s) 649, 740, 1015, 1018, 1133, 1622
Tsp45I GTSAC 4 cut(s) 439, 562, 1487, 1852
TspDTI ATGAA 8 cut(s) 43, 470, 929, 1095, 1143, 1314, 1524, 1808
TspRI CASTG 5 cut(s) 148, 552, 853, 858, 1324
Van91I CCANNNNNTGG 1 cut(s) 280
Vha464I CTTAAG 1 cut(s) 937
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 5 cut(s) 656, 942, 1263, 1420, 1780
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 1750
XceI RCATGY 2 cut(s) 1158, 1336
XmiI GTMKAC 1 cut(s) 1189
XspI CTAG 5 cut(s) 960, 996, 1022, 1256, 1857
Zsp2I ATGCAT 1 cut(s) 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.